Article
Microbiology
Megan M. Dubay, Nikki Johnston, Mark Wronkiewicz, Jake Lee, Christian A. Lindensmith, Jay L. Nadeau
Summary: This study presents a system for investigating bacterial motility at high temperatures using a submerged digital holographic microscope. The researchers observed active motility in Bacillus subtilis up to 66 degrees C, with most cells becoming immobilized as temperatures rose. A fraction of cells remained highly motile at distances above the surface. The study also introduced an automated tracking package for quantifying motility parameters.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Marios Nikolaidis, Andrew Hesketh, Dimitris Mossialos, Ioannis Iliopoulos, Stephen G. Oliver, Grigorios D. Amoutzias
Summary: By analyzing 1104 high-quality genome sequences, this study identifies the core proteins and lineage-specific fingerprint proteins of various evolutionary clusters of the Bacillus genus. The majority of Bacillus species show a low number of species-specific fingerprint proteins, suggesting that species-specific adaptations may be attributed to changes at the gene regulation level. The identification of lineage-specific fingerprints has potential applications in the development of specific vaccines, therapeutic molecules, or molecular tests for species identification.
Article
Food Science & Technology
Wenqiang Shen, Yaojun Liu, Xinyue Zhang, Xiong Zhang, Xiaoping Rong, Lihong Zhao, Cheng Ji, Yuanpei Lei, Fengjuan Li, Jing Chen, Qiugang Ma
Summary: In this study, the potential ameliorative effects of probiotic Bacillus subtilis and biodegradable Bacillus subtilis on ZEN toxicosis in gilts were compared. The results showed that the biodegradable Bacillus subtilis had significant protective effects on growth performance, immune function, hormone secretion, and ZEN elimination in gilts compared to the probiotic Bacillus subtilis.
Article
Multidisciplinary Sciences
Sarah Gingichashvili, Doron Steinberg, Ronit Vogt Sionov, Osnat Feuerstein, Noa E. Cohen
Summary: In this study, computational analytics were used to quantify the morphological response of Bacillus subtilis to the proximity of an antimicrobial source. The results revealed that the inner core, characterized by wrinkled formations, is more preserved compared to the periphery. Additionally, a crescent-shaped colony morphology and changes in the growth substrate of macrocolonies exposed to the antimicrobial were observed.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Mei-yan Wu, Wen-wei Li, Graham Christie, Peter Setlow, Yong-qing Li
Summary: Using Raman tweezers and live-cell phase-contrast microscopy, this study characterized the lysis process of individual Bacillus subtilis cells carrying PBSX prophage during spores' germination, outgrowth, and vegetative growth. The findings showed that nucleic acid intensities in induced cultures decreased rapidly after prophage induction, with a delay before cell burst, and the latent time and burst time varied in cell populations under different culture conditions.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Qilin Yu, Haohao Mao, Bowen Yang, Yahui Zhu, Cheng Sun, Zhiqiang Zhao, Yang Li, Yaobin Zhang
Summary: Electrical stimulation was found to enhance microbial extracellular electron transfer (EET), but the underlying reasons were unclear. This study showed that Bacillus subtilis, a gram-positive bacterium capable of extracellular respiration, had a higher EET capacity after electrical domestication. It was discovered that the polarization of amide groups induced by electrical stimulation promoted H-bond recombination and radical generation of protein-like substances, facilitating extracellular electron transfer via proton-coupled mechanism.
Article
Chemistry, Multidisciplinary
Alice Miral, Sylvain Fournet, Catherine Porte, Aurelie Sauvager, Josselin Montarry, Sophie Tomasi, Sylvain Tranchimand
Summary: Healthy food is a major challenge for the development of this century, with plant-parasitic nematodes causing significant damage to global crop yields. The use of chemical nematicides for control is no longer environmentally sustainable, and biocontrol methods, such as biopesticides derived from living organisms, offer a promising alternative. This study focuses on the underexploited ecological niche of lichen microecosystems to develop new plant protection products. The lichen-associated bacterial suspensions from Paenibacillus etheri are investigated for their potential as nematicides against two nematode species, and the effects of volatile organic compounds produced by the bacteria are evaluated.
Article
Biochemistry & Molecular Biology
Zhiwei Tu, Henk L. Dekker, Winfried Roseboom, Bhagyashree N. Swarge, Peter Setlow, Stanley Brul, Gertjan Kramer
Summary: The research investigated the proteome dynamics during sporulation of Bacillus subtilis vegetative cells under nutrient limitation. Protein co-expression analysis identified four modules, with brown and green modules being upregulated during sporulation containing proteins associated with sporulation, while blue module showed negative correlation and contained ribosomal and metabolic proteins. Besides known transcription regulons, some proteins co-expressed with brown and green modules may also play roles during sporulation. Levels of some coat proteins decreased late in sporulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Marine
Vincenzo Zammuto, Antonio Spano, Marco Sebastiano Nicolo, Emanuela Grillo, Maria Teresa Caccamo, Salvatore Magazu, Simone Cappello, Concetta Gugliandolo
Summary: The exploitation of thermophilic hydrocarbon-utilizing bacilli could provide novel environmentally friendly surfactants. In this study, 80 thermophilic bacilli were isolated from shallow hydrothermal vents and 15 strains were found to be able to utilize hydrocarbons and produce biosurfactants. Two of the selected strains produced surfactants with emulsifying activity and the ability to remove different hydrocarbons and vegetable oils.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Editorial Material
Biochemistry & Molecular Biology
Bing Wang, Irina Artsimovitch
Summary: Bacillus subtilis utilizes uncoupled transcription and translation strategies to meet its unique regulatory needs, showing that bacteria employ diverse gene expression strategies.
TRENDS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Vincent Charron-Lamoureux, Lounes Haroune, Maude Pomerleau, Leo Hall, Frederic Orban, Julie Leroux, Adrien Rizzi, Jean-Sebastien Bourassa, Nicolas Fontaine, Elodie V. d'Astous, Philippe Dauphin-Ducharme, Claude Y. Legault, Jean-Philippe Bellenger, Pascale B. Beauregard
Summary: This study shows that a compound produced by Bacillus subtilis plays a complex role in modulating iron availability and providing protection against oxidative stress during inter-species competition. This compound binds to iron and restricts its availability rather than promoting iron acquisition. It also reduces reactive oxygen species (ROS) formation and oxidative stress, and Bacillus subtilis utilizes its known siderophore to retrieve iron from the compound.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Luciana Fernandes Brito, Marta Irla, Ingemar Naerdal, Simone Balzer Le, Baudoin Delepine, Stephanie Heux, Trygve Brautaset
Summary: This study successfully established a method for methanol-based production of 5-aminovalerate (5AVA) using recombinant Bacillus methanolicus strains. Among the five tested biosynthesis pathways, two pathways achieved the highest titer of 0.02 g l(-1) 5AVA production, making it the first report of 5AVA production from methanol in methylotrophic bacteria.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aisling Brady, Nuria Quiles-Puchalt, Francisca Gallego del Sol, Sara Zamora-Caballero, Alonso Felipe-Ruiz, Jorge Val-Calvo, Wilfried J. J. Meijer, Alberto Marina, Jose R. Penades
Summary: Some Bacillus-infecting bacteriophages utilize a peptide-based communication system, called arbitrium, to coordinate the lysis-lysogeny decision. AimR, a key player in this system, is involved in a complex network of regulation. The SP beta phage, possessing a functional arbitrium system, can optimize production of infective particles while preserving the number of surviving cells, which increases phage persistence in nature.
Article
Biochemistry & Molecular Biology
Marta Irla, Sigrid Hakvag, Trygve Brautaset
Summary: Genome-wide transcriptomic data from RNA-seq experiments can help identify novel regulatory elements. Riboswitches, such as the lysine riboswitch in Bacillus methanolicus, are useful tools for regulating gene expression. Promoters are easily identifiable in prokaryotic genomes and can be combined with riboswitches to create novel tools for inducible gene expression, as demonstrated in this study.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Irene Julca, Pablo Vargas, Toni Gabaldon
Summary: The evolutionary history of the Olea europaea complex involves hybridization and polyploidization events, leading to the formation of new subspecies. The genetic diversity of the species is crucial for its management and preservation of genetic resources.
Article
Biotechnology & Applied Microbiology
Noor Hassan, Barbara Geiger, Rosaria Gandini, Bharat K. C. Patel, Roman Kittl, Dietmar Haltrich, Thu-Ha Nguyen, Christina Divne, Tien Chye Tan
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2016)
Article
Public, Environmental & Occupational Health
S. A. Lajhar, A. V. Jennison, B. Patel, L. L. Duffy
EPIDEMIOLOGY AND INFECTION
(2015)
Article
Biochemical Research Methods
Noor Hassan, Lokesh D. Kori, Rosaria Gandini, Bharat K. C. Patel, Christina Divne, Tien Chye Tan
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2015)
Article
Biotechnology & Applied Microbiology
Joseph Adelskov, Bharat K. C. Patel
Correction
Biotechnology & Applied Microbiology
Joseph Adelskov, Bharat K. C. Patel
Correction
Biotechnology & Applied Microbiology
Joseph Adelskov, Bharat K. C. Patel
Article
Biotechnology & Applied Microbiology
Noor Hassan, Thu-Ha Nguyen, Montira Intanon, Lokesh D. Kori, Bharat K. C. Patel, Dietmar Haltrich, Christina Divne, Tien Chye Tan
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2015)
Article
Environmental Sciences
Frederique Reverchon, Kadum M. Abdullah, Shahla Hosseini Bai, Emanuel Villafan, Timothy J. Blumfield, Bharat Patel, Zhihong Xu
JOURNAL OF SOILS AND SEDIMENTS
(2020)
Article
Biotechnology & Applied Microbiology
Louisa F. James-Pearson, Kevin J. Dudley, Valentino Setoa Junior Teo, Bharat K. C. Patel
Summary: This research was funded by the Queensland University of Technology (QUT) Postgraduate Research Award and the Catapult research funding program, and was supported by the Central Analytical Research Facility (CARF) at QUT and the Nectar Research Cloud.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
N. R. Abady, C. J. D. Guglielmino, R. M. Graham, J. Adelskov, H. V. Smith, B. K. C. Patel, A. V. Jennison
CANADIAN JOURNAL OF MICROBIOLOGY
(2017)
Article
Microbiology
Joseph Adelskov, Bharat K. C. Patel
GENOME ANNOUNCEMENTS
(2015)
Article
Microbiology
Joseph Adelskov, Bharat K. C. Patel
GENOME ANNOUNCEMENTS
(2014)
Article
Bharat K. C. Patel, Valentino Setoa Junior Te'o
GENOME ANNOUNCEMENTS
(2016)
Article
Bharat K. C. Patel
GENOME ANNOUNCEMENTS
(2015)