CRISPR/Cas9-Mediated Genome Editing of Herpesviruses Limits Productive and Latent Infections

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Precise gene deletion and replacement using the CRISPR/Cas9 system in human cells

(2016) Qiupeng Zheng et al.   BIOTECHNIQUES

Elimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing

(2016) Rafal Kaminski et al.   Scientific Reports

Efficient Intracellular Delivery of Native Proteins

(2015) Diego S. D’Astolfo et al.   CELL

Cytomegalovirus in immunocompromised children

(2015) Surabhi B. Vora et al.   CURRENT OPINION IN INFECTIOUS DISEASES

Engineering herpes simplex viruses by infection–transfection methods including recombination site targeting by CRISPR/Cas9 nucleases

(2015) Tiffany A. Russell et al.   JOURNAL OF VIROLOGICAL METHODS

Expanding the Biologist’s Toolkit with CRISPR-Cas9

(2015) Samuel H. Sternberg et al.   MOLECULAR CELL

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

Cas9-mediated targeting of viral RNA in eukaryotic cells

(2015) Aryn A. Price et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells

(2015) Hsin-Kai Liao et al.   Nature Communications

CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust reactivation of the HIV-1 latent reservoirs

(2015) Yonggang Zhang et al.   Scientific Reports

CRISPR/Cas9 System as an Agent for Eliminating Polyomavirus JC Infection

(2015) Hassen S. Wollebo et al.   PLoS One

Small molecule inhibition of Epstein–Barr virus nuclear antigen-1 DNA binding activity interferes with replication and persistence of the viral genome

(2014) Eun Kyung Lee et al.   ANTIVIRAL RESEARCH

In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by CRISPR/Cas9

(2014) Shuai Zhen et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9

(2014) Pankaj K. Mandal et al.   Cell Stem Cell

Herpes simplex keratitis

(2014) Geoffrey M. Hill et al.   DM DISEASE-A-MONTH

Gene transfer of integration defective anti-HSV-1 meganuclease to human corneas ex vivo

(2014) H M Elbadawy et al.   GENE THERAPY

CRISPR/Cas9-mediated genome editing of Epstein-Barr virus in human cells

(2014) K.-S. Yuen et al.   JOURNAL OF GENERAL VIROLOGY

Inactivation of the Human Papillomavirus E6 or E7 Gene in Cervical Carcinoma Cells by Using a Bacterial CRISPR/Cas RNA-Guided Endonuclease

(2014) E. M. Kennedy et al.   JOURNAL OF VIROLOGY

Engineering large viral DNA genomes using the CRISPR-Cas9 system

(2014) Tadahiro Suenaga et al.   MICROBIOLOGY AND IMMUNOLOGY

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

(2014) Xuebing Wu et al.   NATURE BIOTECHNOLOGY

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

(2014) John A Zuris et al.   NATURE BIOTECHNOLOGY

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease

(2014) Cem Kuscu et al.   NATURE BIOTECHNOLOGY

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

RNA-guided endonuclease provides a therapeutic strategy to cure latent herpesviridae infection

(2014) J. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection

(2014) W. Hu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Antiviral drug resistance in herpesviruses other than cytomegalovirus

(2014) Jocelyne Piret et al.   REVIEWS IN MEDICAL VIROLOGY

A high-coverage shRNA screen identifies TMEM129 as an E3 ligase involved in ER-associated protein degradation

(2014) Michael L. van de Weijer et al.   Nature Communications

High-Efficiency Targeted Editing of Large Viral Genomes by RNA-Guided Nucleases

(2014) Yanwei Bi et al.   PLoS Pathogens

In vitro Inactivation of Latent HSV by Targeted Mutagenesis Using an HSV-specific Homing Endonuclease

(2014) Martine Aubert et al.   Molecular Therapy-Nucleic Acids

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering

(2013) Hui Yang et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Birth of a New Therapeutic Platform: 47 Years of Adeno-associated Virus Biology From Virus Discovery to Licensed Gene Therapy

(2013) Terence R Flotte   MOLECULAR THERAPY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Heritable gene targeting in the mouse and rat using a CRISPR-Cas system

(2013) Dali Li et al.   NATURE BIOTECHNOLOGY

Cas9 as a versatile tool for engineering biology

(2013) Prashant Mali et al.   NATURE METHODS

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus

(2013) Hirotaka Ebina et al.   Scientific Reports

Using Tablet for visual exploration of second-generation sequencing data

(2012) I. Milne et al.   BRIEFINGS IN BIOINFORMATICS

RNA-guided genetic silencing systems in bacteria and archaea

(2012) Blake Wiedenheft et al.   NATURE

Fast gapped-read alignment with Bowtie 2

(2012) Ben Langmead et al.   NATURE METHODS

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Activities of ICP0 Involved in the Reversal of Silencing of Quiescent Herpes Simplex Virus 1

(2011) M. W. Ferenczy et al.   JOURNAL OF VIROLOGY

Roscovitine Inhibits EBNA1 Serine 393 Phosphorylation, Nuclear Localization, Transcription, and Episome Maintenance

(2011) M.-S. Kang et al.   JOURNAL OF VIROLOGY

CRISPR/Cas, the Immune System of Bacteria and Archaea

(2010) P. Horvath et al.   SCIENCE

UL84-independent replication of human cytomegalovirus strain TB40/E

(2010) David J. Spector et al.   VIROLOGY

The Sequence Alignment/Map format and SAMtools

(2009) H. Li et al.   BIOINFORMATICS

Evolutionary analysis of the dynamics of viral infectious disease

(2009) Oliver G. Pybus et al.   NATURE REVIEWS GENETICS

Histone modifications associated with herpes simplex virus type 1 genomes during quiescence and following ICP0-mediated de-repression

(2007) H. M. Coleman et al.   JOURNAL OF GENERAL VIROLOGY