Article
Biochemistry & Molecular Biology
Manuel Grander, Alexander Hoffmann, Markus Seifert, Egon Demetz, Philipp Grubwieser, Christa Pfeifhofer-Obermair, David Haschka, Guenter Weiss
Summary: Macrophages play a crucial role in innate immunity by controlling iron trafficking and infection. The study shows that DMT1 is essential for iron uptake and storage in macrophages, and its absence leads to impaired control of siderophilic bacteria infection. This highlights the importance of DMT1 in nutritional immunity and iron delivery for infection control.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hyeong Seok An, Jung-Wan Yoo, Jong Hwan Jeong, Manbong Heo, Si Hwan Hwang, Hye Min Jang, Eun Ae Jeong, Jaewoong Lee, Hyun Joo Shin, Kyung Eun Kim, Meong Cheol Shin, Gu Seob Roh
Summary: Lipocalin-2 (LCN2) is an acute-phase protein that regulates inflammatory responses to bacteria or lipopolysaccharide (LPS). This study found that LCN2 plays a key role in inflammation and oxidative stress following acute lung injury and may be a potential therapeutic target for pneumonia or acute lung injury.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Hye Young Kim, Young Hyun Yoo
Summary: Recent studies have shown that exposure to polychlorinated biphenyls (PCB) leads to toxicant-associated steatohepatitis, but the underlying mechanism is not yet understood. In this study, mice were exposed to PCB through intraperitoneal injection and exhibited hepatic injury, steatosis, inflammation, and fibrosis. It was also observed that PCB exposure led to hepatic iron overload. Furthermore, the expression of hepatic lipocalin-2 (LCN2) was significantly increased in the PCB-induced models of nonalcoholic fatty liver disease (NAFLD)/nonalcoholic steatohepatitis (NASH).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dermatology
Jingyi Ma, Jiaoling Chen, Ke Xue, Chen Yu, Erle Dang, Hongjiang Qiao, Hui Fang, Bingyu Pang, Qingyang Li, Zhongbin Sun, Pei Qiao, Lei Wang, Gang Wang, Shuai Shao
Summary: This study reveals the critical role of LCN2 in psoriasis, showing that it activates keratinocytes through the SRBP2-NLRC4 axis and leads to inflammatory responses. This provides a potential therapeutic target for the treatment of psoriasis.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Review
Oncology
Maida Zivalj, Jo A. Van Ginderachter, Benoit Stijlemans
Summary: The aim of this review is to summarize the available information regarding Lipocalin-2 in tumor progression. Lipocalin-2 expression is correlated with tumorigenesis and can be considered as a biomarker and potential drug target in cancer therapy.
Article
Cardiac & Cardiovascular Systems
Yuxue Huang, Ning Zhang, Cuiping Xie, Yayu You, Lei Guo, Feiming Ye, Xiaojie Xie, Jian'an Wang
Summary: This study found that LCN2 deficiency prevented sepsis-induced cardiac dysfunction and ferroptosis, and suggested that neutrophil-derived LCN2 might be a potential therapeutic target for SCD.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Neurosciences
Daejin Lim, Jae-ho Jeong, Juhyun Song
Summary: Dementia accompanied by memory loss is a common neurodegenerative disease with increasing prevalence worldwide. Recent evidence suggests that lipocalin 2 (LCN2) plays a regulatory role in dementia and may be a promising therapeutic target to address the neuropathology of dementia.
CNS NEUROSCIENCE & THERAPEUTICS
(2021)
Article
Cell Biology
Sirinart Kumfu, Natthaphat Siri-Angkul, Siriporn C. Chattipakorn, Nipon Chattipakorn
Summary: This study investigated the roles of LCN-2 and LCN-2 receptors as iron transporters in cardiomyocytes under iron overload conditions. LTCC and TTCC were found to play crucial roles in Fe2+ uptake, while DFP showed beneficial effects in both Fe2+ and Fe3+ overload conditions.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Eun Bee Choi, Jae Hun Jeong, Hye Min Jang, Yu Jeong Ahn, Kyu Hyeon Kim, Hyeong Seok An, Jong Youl Lee, Eun Ae Jeong, Jaewoong Lee, Hyun Joo Shin, Kyung Eun Kim, Gu Seob Roh
Summary: The study revealed that elevated levels of LCN2 in skeletal muscle of obese individuals are associated with inflammation and oxidative stress related to muscle atrophy.
Article
Biochemistry & Molecular Biology
Yi-Chinn Weng, Yu-Ting Huang, I-Chen Chiang, Pei-Ju Tsai, Yu-Wen Su, Wen-Hai Chou
Summary: The study found that LCN2 has neurotoxic effects on engrafted neurons during neural transplantation and can also modulate neuroinflammatory responses. Inhibition of LCN2 may be useful in reducing rejection after neural transplantation.
Article
Engineering, Environmental
Bing Zhao, Lingbo Zhao, Peng Gao, Yuexin Han, Yanjun Li, Zaizheng Dong, Yan Song
Summary: Cyanide detoxification and resource utilization of cyanide tailings were achieved through oxidation-reduction roasting. The content of cyanide was reduced to <0.04 mg/kg with a removal rate of 99.99% under optimal conditions during oxidation roasting. Reduction roasting transformed the main mineral hematite to magnetite and recovered iron concentrate from the cyanide tailings at an iron grade of 59.21% and iron recovery of 89.57%. The migration, transformation paths, and decomposition products of cyanide were investigated through hexacyanoferrate reagents decomposition, real-time CO2 concentration, XRD, and FT-IR analysis.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Neurosciences
Jae-Hong Kim, Osung Kwon, Anup Bhusal, Jiyoun Lee, Eun Mi Hwang, Hoon Ryu, Jae-Yong Park, Kyoungho Suk
Summary: This study reports the generation and characterization of conditional transgenic mice for lipocalin-2 (LCN2) expression. By delivering Cre recombinase under the control of a GFAP promoter, targeted overexpression of LCN2 in astrocytes of the hippocampus was achieved. These mice showed neuroinflammation, enhanced glial activation, increased proinflammatory cytokines, and impaired cognitive functions, providing a new in vivo model for neuroinflammation.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Christina Mertens, Matthias Schnetz, Claudia Rehwald, Stephan Grein, Eiman Elwakeel, Andreas Weigert, Bernhard Bruene, Michaela Jung
Summary: Macrophages in the tumor microenvironment contribute to tumor growth by secreting Lcn-2-bound iron and upregulating FPN expression. Lcn-2 plays a crucial role in tumor iron-management, affecting tumor growth progression.
Article
Infectious Diseases
David Haschka, Manuel Grander, Johannes Eibensteiner, Stefanie Dichtl, Sabine Koppelstaetter, Guenter Weiss
Summary: The calcium channel blocker nifedipine limits intracellular pathogens by inducing cellular iron export. Combined with antibiotics, nifedipine shows an additive effect against Salmonella. Bacteria with reduced access to iron exhibit increased sensitivity to treatment with antibiotics or nifedipine.
Article
Cell Biology
Xin Wen, Bin Su, Mingming Gao, Jiaqi Chen, Donglei Zhou, Hui You, Nannan Li, Shuaikang Chang, Xiaoyun Cheng, Chunhua Qian, Jingyang Gao, Peng Yang, Shen Qu, Le Bu
Summary: Our study reveals that lipocalin 2 (LCN2) plays a protective role in obese individuals, inhibiting cell apoptosis and improving inflammatory state by repressing endoplasmic reticulum stress, thereby alleviating gastric injury associated with obesity.
CELL DEATH & DISEASE
(2021)
Article
Agriculture, Multidisciplinary
Zhi-Wei Hou, Chen-Hui Chen, Jia-Ping Ke, Yuan-Yuan Zhang, Yan Qi, Shi-Yu Liu, Zi Yang, Jing-Ming Ning, Guan-Hu Bao
Summary: Flavoalkaloids, a unique class of compounds in tea, have been isolated from Yingde green tea, with four novel spiro-flavoalkaloids identified. These compounds show potential alpha-glucosidase inhibitory activities.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Fang Hua, Peng Zhou, Guan-hu Bao, Tie-jun Ling
Summary: Current studies have found that plasma trimethylamine N-oxide (TMAO) level is positively correlated with the risk of acute myocardial infarction (AMI). Flavonoids in Lu'an GuaPian tea, especially kaempferol 3-O-rutinoside, have shown potential in inhibiting TMA production and could be used for AMI treatment.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Zi Yang, Li Li, Chen-Hui Chen, Yuan-Yuan Zhang, Yi Yang, Peng Zhang, Guan-Hu Bao
Summary: The study evaluated the antibacterial activities of 12 medicinal plant extracts against six foodborne bacteria, with Toona sinensis shoots and Firmiana simplex barks showing the highest inhibitory activities against various strains. Firmiana simplex barks exhibited strong antibacterial effects against Salmonella typhi. Furthermore, the identified bioactive compounds in the plant extracts were mainly polyphenols, glycosides, and terpenoids.
PHYTOCHEMICAL ANALYSIS
(2022)
Article
Plant Sciences
Shi-Yu Liu, Wei Wang, Jia-Ping Ke, Peng Zhang, Gang-Xiu Chu, Guan-Hu Bao
Summary: This study investigated the inhibitory effect of catechins from tea on 3-chymotrypsin-like cysteine protease (3CLpro). The results showed that catechins interacted with 3CLpro through specific amino acid residues, and some catechins exhibited intracellular inhibitory activity against 3CLpro.
Article
Biochemistry & Molecular Biology
Pu Wang, Yihai Huang, Jiayue Ren, Yuezhao Rong, Lili Fan, Peng Zhang, Xueying Zhang, Junxiao Xi, Shuying Mao, Min Su, Baobao Zhang, Guanhu Bao, Feihua Wu
Summary: The n-butanol fraction of Huoshan large-leaf yellow tea extract (HLYTBE) protects vascular endothelial cells from high glucose-induced injury by upregulating autophagy and inhibiting oxidative stress.
Article
Biotechnology & Applied Microbiology
Jie Wei, Xue Zhou, Mei Dong, Lufan Yang, Cheng Zhao, Ruili Lu, Guanhu Bao, Fenglin Hu
Summary: High-resolution mass spectrometry and nuclear magnetic resonance analysis identified 20 main components in the spores and mycelia extract of Cordyceps fumosorosea strain RCEF 6672, including novel compounds with antibacterial, antifungal, and antiaging activities. These secondary metabolites have potential for pharmaceutical development.
Article
Biochemistry & Molecular Biology
Fang Hua, Jing Ya Li, Meng Zhang, Peng Zhou, Liang Wang, Tie Jun Ling, Guan Hu Bao
Summary: This study demonstrates that Kaempferol-3-O-rutinoside (KR) can prevent and treat ventricular remodeling (VR) after acute myocardial infarction (AMI) through regulating the NE-kappa B/NLRP3/Caspase-1 signaling pathway, resulting in improved cardiac function.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Chemistry, Physical
Jie Wei, Cheng Zhao, Guiying Ji, Mei Dong, Ruili Lu, Bo Huang, Guanhu Bao, Fenglin Hu
Summary: In this study, a pair of novel enantiomers, metarrhiziumoids A and B, were isolated from the extract of Metarhizium flavoviride. The structures of the compounds were identified using various techniques. The compounds showed glycosidase inhibitory activity, with metarrhiziumoids B exhibiting stronger activity than metarrhiziumoids A. Molecular docking results revealed different binding energies between the two enantiomers, which may contribute to the differences in inhibitory activity.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Chemistry, Applied
Yuan -Yuan Zhang, Peng Zhang, Miao-Miao Le, Yan Qi, Zi Yang, Feng-Lin Hu, Tie-Jun Ling, Guan-Hu Bao
Summary: This study aimed to improve the flavor of summer Keemun black tea (KBT) using Cordyceps militaris solid-state fermentation. The analysis of volatile and non-volatile components revealed that the total polyphenol, total flavonoid, and total free amino acid contents were reduced after solid-state fermentation. Additionally, the contents of non-galloylated catechins and D-mannitol increased, while the galloylated catechins and flavonoid glycosides decreased, leading to a decrease in bitterness and astringency of KBT. Aroma compounds such as dihydro-beta-ionone and beta-ionone were identified as the key contributors to the woody and floral odors in the fermented KBT.
Article
Agriculture, Multidisciplinary
Guangjin Liu, Kelin Huang, Jiaping Ke, Chenhui Chen, Guan-Hu Bao, Xiaochun Wan
Summary: This study identified a novel O-methyltransferase (CsOMTL1) responsible for the synthesis of EGCG4''Me, and a transcription factor (CsMADSL1) that activates the transcription of CsOMTL1. It was found that EGCG4''Me is characteristically accumulated in the GZMe4 cultivar.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Cheng Zhao, Jiaojiao Qu, Ruili Lu, Renjie Chen, Quheng Dong, Bo Huang, Guanhu Bao, Fenglin Hu
Summary: Nine undescribed cyclic pentapeptides, named basidiosin D-L, were isolated from mycelia of B. meristosporus RCEF4516 using semi-preparative HPLC. These compounds showed inhibitory effects on NO production in LPS-activated RAW264.7 cells and cytotoxicity against RAW264.7, 293 T, and HepG2 cells. Additionally, they exhibited stronger inhibitory effects on alpha-glucosidase compared to acarbose, except for compound 7.
Article
Respiratory System
Johanna Salonen, Hanna Nurmi, Ulla Hodgson, Hannele Hasala, Maritta Kilpeläinen, Maria Hollmen, Minna Purokivi, Riitta Kaarteenaho
Summary: This study investigated the implementation and key elements of Interstitial Lung Diseases (ILD) Multidisciplinary Meetings (MDMs) in Finnish specialized care. ILD MDMs were organized approximately every two weeks in all academic centers of Finland, with key participants including a radiologist, respiratory physicians, junior staff, pathologist, and a rheumatologist. Virtual communication was commonly used in ILD MDM implementation, particularly in small centers located in Eastern and Northern Finland. Virtual access to ILD MDMs of academic centers was available in most parts of Finland, enabling small centers to benefit from their expertise.
EUROPEAN CLINICAL RESPIRATORY JOURNAL
(2023)
Article
Chemistry, Applied
Chen-Hui Chen, Jing-Ya Yu, Zi Yang, Jia-Ping Ke, Yan Qi, Yi Yang, Biao Gao, Guangmin Yao, Guan-Hu Bao
Summary: Methylation is a common modification of catechins in tea, and this study isolated three new methylated catechins from Echa 1 green tea. Two of the methylated catechins showed strong inhibitory activity against alpha-glucosidase and significantly reduced fat content in Caenorhabditis elegans.
Article
Plant Sciences
Cheng Zhao, Jiaojiao Qu, Fan Peng, Ruili Lu, Guan-Hu Bao, Bo Huang, Fenglin Hu
Summary: Three new cyclic peptides, meristosporins A, B, and C (1-3), with unique amino acid composition, were isolated from Basidiobolus meristosporus, an opportunistic pathogen. The structures of these compounds were determined using advanced analytical techniques. The biosynthetic pathway of meristosporin A (1) was inferred based on genetic analysis. Compounds 1 and 2 exhibited cytotoxicity towards RAW264.7 and 293T cells, respectively, suggesting their potential involvement in fungal-induced cell injury.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Jia-Ping Ke, Jing-Ya Yu, Biao Gao, Feng-Lin Hu, Feng-Qing Xu, Guangmin Yao, Guan-Hu Bao
Summary: Two new catechins, zijuanins E and F, were isolated and identified from Zijuan green tea. These compounds were found to prolong the lifespan of Caenorhabditis elegans, enhance stress resistance, and reduce oxidative stress. They also exhibited strong antioxidant activity and binding ability to human serum albumin.