Thermal proteome profiling: unbiased assessment of protein state through heat-induced stability changes

Monitoring Cell-surfaceN-Glycoproteome Dynamics by Quantitative Proteomics Reveals Mechanistic Insights into Macrophage Differentiation

(2017) Mathias Kalxdorf et al.   MOLECULAR & CELLULAR PROTEOMICS

Cell-wide analysis of protein thermal unfolding reveals determinants of thermostability

(2017) Pascal Leuenberger et al.   SCIENCE

Validation and development of MTH1 inhibitors for treatment of cancer

(2016) U. Warpman Berglund et al.   ANNALS OF ONCOLOGY

Identifying compound efficacy targets in phenotypic drug discovery

(2016) Markus Schirle et al.   DRUG DISCOVERY TODAY

An Unbiased Chemical Proteomics Method Identifies FabI as the Primary Target of 6-OH-BDE-47

(2016) Hui Peng et al.   ENVIRONMENTAL SCIENCE & TECHNOLOGY

Cytotoxic and Proinflammatory Effects of Metal-Based Nanoparticles on THP-1 Monocytes Characterized by Combined Proteomics Approaches

(2016) Nataliya K. Tarasova et al.   JOURNAL OF PROTEOME RESEARCH

Mass-spectrometric exploration of proteome structure and function

(2016) Ruedi Aebersold et al.   NATURE

Thermal profiling reveals phenylalanine hydroxylase as an off-target of panobinostat

(2016) Isabelle Becher et al.   Nature Chemical Biology

The MaxQuant computational platform for mass spectrometry-based shotgun proteomics

(2016) Stefka Tyanova et al.   Nature Protocols

A comprehensive map of molecular drug targets

(2016) Rita Santos et al.   NATURE REVIEWS DRUG DISCOVERY

Label-free technologies for target identification and validation

(2016) Jing Li et al.   MedChemComm

CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil

(2016) Helena Almqvist et al.   Nature Communications

The resurgence of phenotypic screening in drug discovery and development

(2015) Bridget K. Wagner   Expert Opinion on Drug Discovery

Application of Mass Spectrometry Profiling to Establish Brusatol as an Inhibitor of Global Protein Synthesis

(2015) Steffan Vartanian et al.   MOLECULAR & CELLULAR PROTEOMICS

Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling

(2015) Kilian V M Huber et al.   NATURE METHODS

Thermal proteome profiling monitors ligand interactions with cellular membrane proteins

(2015) Friedrich B M Reinhard et al.   NATURE METHODS

Thermal proteome profiling for unbiased identification of direct and indirect drug targets using multiplexed quantitative mass spectrometry

(2015) Holger Franken et al.   Nature Protocols

Multidimensional proteomics for cell biology

(2015) Mark Larance et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Ion Coalescence of Neutron Encoded TMT 10-Plex Reporter Ions

(2014) Thilo Werner et al.   ANALYTICAL CHEMISTRY

MTH1 inhibition eradicates cancer by preventing sanitation of the dNTP pool

(2014) Helge Gad et al.   NATURE

The cellular thermal shift assay for evaluating drug target interactions in cells

(2014) Rozbeh Jafari et al.   Nature Protocols

Phenotypic screening in cancer drug discovery — past, present and future

(2014) John G. Moffat et al.   NATURE REVIEWS DRUG DISCOVERY

Tracking cancer drugs in living cells by thermal profiling of the proteome

(2014) M. M. Savitski et al.   SCIENCE

Orbitrap Mass Spectrometry

(2013) Roman A. Zubarev et al.   ANALYTICAL CHEMISTRY

Phenotypic screens as a renewed approach for drug discovery

(2013) Wei Zheng et al.   DRUG DISCOVERY TODAY

Target identification and mechanism of action in chemical biology and drug discovery

(2013) Monica Schenone et al.   Nature Chemical Biology

Monitoring Drug Target Engagement in Cells and Tissues Using the Cellular Thermal Shift Assay

(2013) D. M. Molina et al.   SCIENCE

Engineering protein thermostability using a generic activity-independent biophysical screen inside the cell

(2013) Ignacio Asial et al.   Nature Communications

High-Resolution Enabled TMT 8-plexing

(2012) Thilo Werner et al.   ANALYTICAL CHEMISTRY

Increasing the Multiplexing Capacity of TMTs Using Reporter Ion Isotopologues with Isobaric Masses

(2012) Graeme C. McAlister et al.   ANALYTICAL CHEMISTRY

Thermodynamic analysis of protein-ligand binding interactions in complex biological mixtures using the stability of proteins from rates of oxidation

(2012) Erin C Strickland et al.   Nature Protocols

How were new medicines discovered?

(2011) David C. Swinney et al.   NATURE REVIEWS DRUG DISCOVERY

Principles of early drug discovery

(2010) JP Hughes et al.   BRITISH JOURNAL OF PHARMACOLOGY

Quantitative proteomics approach for identifying protein-drug interactions in complex mixtures using protein stability measurements

(2010) G. M. West et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Pervanadate stabilizes desmosomes

(2010) David R. Garrod et al.   Cell Adhesion & Migration

Target identification using drug affinity responsive target stability (DARTS)

(2009) B. Lomenick et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Thermodynamic Analysis of Protein Stability and Ligand Binding Using a Chemical Modification- and Mass Spectrometry-Based Strategy

(2008) Graham M. West et al.   ANALYTICAL CHEMISTRY

Relative Quantification of Proteins in Human Cerebrospinal Fluids by MS/MS Using 6-Plex Isobaric Tags

(2008) Loïc Dayon et al.   ANALYTICAL CHEMISTRY

MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification

(2008) Jürgen Cox et al.   NATURE BIOTECHNOLOGY