期刊
MICROBIAL CELL FACTORIES
卷 18, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12934-019-1112-2
关键词
CRISPR-Cas9; Non-conventional yeasts; Genome editing; Guide RNA; Homologous recombination; Non-homologous end joining
资金
- National Natural Science Foundation of China [31700082, 21808216]
- DMTO research grant from Dalian Institute of Chemicals Physics, CAS [DICP DMTO201701]
Non-conventional yeasts are playing important roles as cell factories for bioproduction of biofuels, food additives and proteins with outstanding natural characteristics. However, the precise genome editing is challenging in non-conventional yeasts due to lack of efficient genetic tools. In the past few years, CRISPR-based genome editing worked as a revolutionary tool for genetic engineering and showed great advantages in cellular metabolic engineering. Here, we review the current advances and barriers of CRISPR-Cas9 for genome editing in non-conventional yeasts and propose the possible solutions in enhancing its efficiency for precise genetic engineering.
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