Cellulosomes: bacterial nanomachines for dismantling plant polysaccharides

Nanoscale Engineering of Designer Cellulosomes

(2016) Melissabye Gunnoo et al.   ADVANCED MATERIALS

Insights into the functionality and stability of designer cellulosomes at elevated temperatures

(2016) Anastasia P. Galanopoulou et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enhancement of cellulosome-mediated deconstruction of cellulose by improving enzyme thermostability

(2016) Sarah Moraïs et al.   Biotechnology for Biofuels

Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824

(2016) Benjamin J. Willson et al.   Biotechnology for Biofuels

Cellulosomal expansin: functionality and incorporation into the complex

(2016) Lior Artzi et al.   Biotechnology for Biofuels

Engineering yeast with bifunctional minicellulosome and cellodextrin pathway for co-utilization of cellulose-mixed sugars

(2016) Li-Hai Fan et al.   Biotechnology for Biofuels

Single versus dual-binding conformations in cellulosomal cohesin–dockerin complexes

(2016) Michael A Nash et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Cellulose degradation in the human gut: Ruminococcus champanellensis expands the cellulosome paradigm

(2016) Isaac Cann et al.   ENVIRONMENTAL MICROBIOLOGY

Toward combined delignification and saccharification of wheat straw by a laccase-containing designer cellulosome

(2016) Lital Davidi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature

(2016) Johanna Stern et al.   mBio

Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase

(2015) Tao Xu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation

(2015) Chao Chen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Combining free and aggregated cellulolytic systems in the cellulosome-producing bacterium Ruminiclostridium cellulolyticum

(2015) Julie Ravachol et al.   Biotechnology for Biofuels

Elimination of hydrogenase active site assembly blocks H2 production and increases ethanol yield in Clostridium thermocellum

(2015) Ranjita Biswas et al.   Biotechnology for Biofuels

Enzymatic profiling of cellulosomal enzymes from the human gut bacterium, R uminococcus champanellensis , reveals a fine-tuned system for cohesin-dockerin recognition

(2015) Sarah Moraïs et al.   ENVIRONMENTAL MICROBIOLOGY

Ruminococcal cellulosomes: molecular Lego to deconstruct microcrystalline cellulose in human gut

(2015) Gurvan Michel   ENVIRONMENTAL MICROBIOLOGY

Ruminococcal cellulosome systems from rumen to human

(2015) Yonit Ben David et al.   ENVIRONMENTAL MICROBIOLOGY

Quantitative proteomic analysis of the cellulolytic system of Clostridium termitidis CT1112 reveals distinct protein expression profiles upon growth on α-cellulose and cellobiose

(2015) Riffat I. Munir et al.   Journal of Proteomics

Elimination of metabolic pathways to all traditional fermentation products increases ethanol yields in Clostridium thermocellum

(2015) Beth Papanek et al.   METABOLIC ENGINEERING

Consolidated bioprocessing of cellulose to isobutanol using Clostridium thermocellum

(2015) Paul P. Lin et al.   METABOLIC ENGINEERING

Mapping Mechanical Force Propagation through Biomolecular Complexes

(2015) Constantin Schoeler et al.   NANO LETTERS

Significance of Relative Position of Cellulases in Designer Cellulosomes for Optimized Cellulolysis

(2015) Johanna Stern et al.   PLoS One

Clostridium clariflavum : Key Cellulosome Players Are Revealed by Proteomic Analysis

(2015) Lior Artzi et al.   mBio

Unique Organization of Extracellular Amylases into Amylosomes in the Resistant Starch-Utilizing Human Colonic Firmicutes Bacterium Ruminococcus bromii

(2015) Xiaolei Ze et al.   mBio

Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization

(2015) Chenggang Xu et al.   Nature Communications

Exoproteome analysis of Clostridium cellulovorans in natural soft-biomass degradation

(2015) Kohei Esaka et al.   AMB Express

Driving biomass breakdown through engineered cellulosomes

(2015) Sean P Gilmore et al.   Bioengineered

Soil and Rhizosphere Associated Fungi in Gray Mangroves (Avicennia marina) from the Red Sea — A Metagenomic Approach

(2015) Marta Filipa Simões et al.   GENOMICS PROTEOMICS & BIOINFORMATICS

Signal amplification by a self-assembled biosensor system designed on the principle of dockerin–cohesin interactions in a cellulosome complex

(2014) Jeong Eun Hyeon et al.   ANALYST

Engineered Pentafunctional Minicellulosome for Simultaneous Saccharification and Ethanol Fermentation in Saccharomyces cerevisiae

(2014) Youyun Liang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Xylanase and cellulase systems of Clostridium sp.: An insight on molecular approaches for strain improvement

(2014) Leya Thomas et al.   BIORESOURCE TECHNOLOGY

New insights into enzymatic hydrolysis of heterogeneous cellulose by using carbohydrate-binding module 3 containing GFP and carbohydrate-binding module 17 containing CFP

(2014) Shuhong Gao et al.   Biotechnology for Biofuels

The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons

(2014) Wei Hong et al.   Biotechnology for Biofuels

Challenges and advances in the heterologous expression of cellulolytic enzymes: a review

(2014) Camilla Lambertz et al.   Biotechnology for Biofuels

Comparative analysis of the ability of Clostridium clariflavum strains and Clostridium thermocellumto utilize hemicellulose and unpretreated plant material

(2014) Javier A Izquierdo et al.   Biotechnology for Biofuels

Cellulosomics of the cellulolytic thermophile Clostridium clariflavum

(2014) Lior Artzi et al.   Biotechnology for Biofuels

A combined cell-consortium approach for lignocellulose degradation by specialized Lactobacillus plantarum cells

(2014) Sarah Moraïs et al.   Biotechnology for Biofuels

Characterization of All Family-9 Glycoside Hydrolases Synthesized by the Cellulosome-producing BacteriumClostridium cellulolyticum

(2014) Julie Ravachol et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Incorporation of Nasutitermes takasagoensis Endoglucanase into Cell Surface-Displayed Minicellulosomes in Pichia pastoris X33

(2014) Jingshen Ou et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Revisiting the NMR solution structure of the Cel48S type-I dockerin module from Clostridium thermocellum reveals a cohesin-primed conformation

(2014) Chao Chen et al.   JOURNAL OF STRUCTURAL BIOLOGY

Rumen Cellulosomics: Divergent Fiber-Degrading Strategies Revealed by Comparative Genome-Wide Analysis of Six Ruminococcal Strains

(2014) Bareket Dassa et al.   PLoS One

Integration of bacterial lytic polysaccharide monooxygenases into designer cellulosomes promotes enhanced cellulose degradation

(2014) Y. Arfi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrastable cellulosome-adhesion complex tightens under load

(2014) Constantin Schoeler et al.   Nature Communications

Elaborate cellulosome architecture ofAcetivibrio cellulolyticusrevealed by selective screening of cohesin–dockerin interactions

(2014) Yuval Hamberg et al.   PeerJ

Global transcriptome analysis of Clostridium thermocellum ATCC 27405 during growth on dilute acid pretreated Populus and switchgrass

(2013) Charlotte M Wilson et al.   Biotechnology for Biofuels

A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates

(2013) Yael Vazana et al.   Biotechnology for Biofuels

Structure and regulation of the cellulose degradome in Clostridium cellulolyticum

(2013) Chenggang Xu et al.   Biotechnology for Biofuels

Aligning an endoglucanase Cel5A from Thermobifida fusca on a DNA scaffold: potent design of an artificial cellulosome

(2013) Yutaro Mori et al.   CHEMICAL COMMUNICATIONS

Insights into cellulosome assembly and dynamics: from dissection to reconstruction of the supramolecular enzyme complex

(2013) Steven P Smith et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Fungal cellulases and complexed cellulosomal enzymes exhibit synergistic mechanisms in cellulose deconstruction

(2013) Michael G. Resch et al.   Energy & Environmental Science

Unraveling enzyme discrimination during cellulosome assembly independent of cohesin-dockerin affinity

(2013) Romain Borne et al.   FEBS Journal

Small Angle X-ray Scattering Analysis ofClostridium thermocellumCellulosome N-terminal Complexes Reveals a Highly Dynamic Structure

(2013) Mark A. Currie et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Atypical Cohesin-Dockerin Complex Responsible for Cell Surface Attachment of Cellulosomal Components

(2013) Orly Salama-Alber et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Development of a Multipoint Quantitation Method to Simultaneously Measure Enzymatic and Structural Components of the Clostridium thermocellum Cellulosome Protein Complex

(2013) Andrew B. Dykstra et al.   JOURNAL OF PROTEOME RESEARCH

The carbohydrate-active enzymes database (CAZy) in 2013

(2013) Vincent Lombard et al.   NUCLEIC ACIDS RESEARCH

Crystal Structure of an Uncommon Cellulosome-Related Protein Module from Ruminococcus flavefaciens That Resembles Papain-Like Cysteine Peptidases

(2013) Maly Levy-Assaraf et al.   PLoS One

Mining of Novel Thermo-Stable Cellulolytic Genes from a Thermophilic Cellulose-Degrading Consortium by Metagenomics

(2013) Yu Xia et al.   PLoS One

Expression of Cellulosome Components and Type IV Pili within the Extracellular Proteome of Ruminococcus flavefaciens 007

(2013) Maša Vodovnik et al.   PLoS One

Cellulases for biomass degradation: comparing recombinant cellulase expression platforms

(2013) Megan Garvey et al.   TRENDS IN BIOTECHNOLOGY

Annexation of a High-Activity Enzyme in a Synthetic Three-Enzyme Complex Greatly Decreases the Degree of Substrate Channeling

(2013) Chun You et al.   ACS Synthetic Biology

Use of substructure-specific carbohydrate binding modules to track changes in cellulose accessibility and surface morphology during the amorphogenesis step of enzymatic hydrolysis

(2012) Keith Gourlay et al.   Biotechnology for Biofuels

Genome-wide analysis of Acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system

(2012) Bareket Dassa et al.   BMC GENOMICS

Ruminococcus bromii is a keystone species for the degradation of resistant starch in the human colon

(2012) Xiaolei Ze et al.   ISME Journal

Scaffoldin Conformation and Dynamics Revealed by a Ternary Complex from theClostridium thermocellumCellulosome

(2012) Mark A. Currie et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Enhanced Cellulose Degradation Using Cellulase-Nanosphere Complexes

(2012) Craig Blanchette et al.   PLoS One

Single-molecule dissection of the high-affinity cohesin-dockerin complex

(2012) S. W. Stahl et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enhanced cellulose degradation by targeted integration of a cohesin-fused  -glucosidase into the Clostridium thermocellum cellulosome

(2012) G. Gefen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production of functional agarolytic nano-complex for the synergistic hydrolysis of marine biomass and its potential application in carbohydrate-binding module-utilizing one-step purification

(2012) Jeong Eun Hyeon et al.   PROCESS BIOCHEMISTRY

How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?

(2012) S.-Y. Ding et al.   SCIENCE

A nanocluster design for the construction of artificial cellulosomes

(2012) Do-Myoung Kim et al.   Catalysis Science & Technology

Complete Genome Sequence of Clostridium clariflavum DSM 19732

(2012) Javier A. Izquierdo et al.   Standards in Genomic Sciences

Self-Assembly of Synthetic Metabolons through Synthetic Protein Scaffolds: One-Step Purification, Co-immobilization, and Substrate Channeling

(2012) Chun You et al.   ACS Synthetic Biology

Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources

(2012) Hironobu Morisaka et al.   AMB Express

Assembly of Minicellulosomes on the Surface of Bacillus subtilis

(2011) Timothy D. Anderson et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A celluloytic complex from Clostridium cellulovorans consisting of mannanase B and endoglucanase E has synergistic effects on galactomannan degradation

(2011) Sang Duck Jeon et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Transcriptomic analysis of Clostridium thermocellum ATCC 27405 cellulose fermentation

(2011) Babu Raman et al.   BMC MICROBIOLOGY

Production of minicellulosomes for the enhanced hydrolysis of cellulosic substrates by recombinant Corynebacterium glutamicum

(2011) Jeong Eun Hyeon et al.   ENZYME AND MICROBIAL TECHNOLOGY

Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum

(2011) S. D. Brown et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Metagenomic Discovery of Biomass-Degrading Genes and Genomes from Cow Rumen

(2011) M. Hess et al.   SCIENCE

Microbial enzyme systems for biomass conversion: emerging paradigms

(2011) Michael E Himmel et al.   Biofuels-UK

Using a microorganism consortium for consolidated bioprocessing cellulosic ethanol production

(2011) Ran Du et al.   Biofuels-UK

Enzyme inactivation by ethanol and development of a kinetic model for thermophilic simultaneous saccharification and fermentation at 50 °C withThermoanaerobacterium saccharolyticumALK2

(2010) Kara K. Podkaminer et al.   BIOTECHNOLOGY AND BIOENGINEERING

The cellulose-degrading microbial community of the human gut varies according to the presence or absence of methanogens

(2010) Christophe Chassard et al.   FEMS MICROBIOLOGY ECOLOGY

The unique set of putative membrane-associated anti-σ factors in Clostridium thermocellum suggests a novel extracellular carbohydrate-sensing mechanism involved in gene regulation

(2010) Hamutal Kahel-Raifer et al.   FEMS MICROBIOLOGY LETTERS

Analysis of cohesin-dockerin interactions using mutant dockerin proteins

(2010) Kazutaka Sakka et al.   FEMS MICROBIOLOGY LETTERS

Enhanced cellulose degradation by nano-complexed enzymes: Synergism between a scaffold-linked exoglucanase and a free endoglucanase

(2010) Sarah Moraïs et al.   JOURNAL OF BIOTECHNOLOGY

Synergy, Structure and Conformational Flexibility of Hybrid Cellulosomes Displaying Various Inter-cohesins Linkers

(2010) Anne-Laure Molinier et al.   JOURNAL OF MOLECULAR BIOLOGY

Characterization of a dockerin-based affinity tag: application for purification of a broad variety of target proteins

(2010) Alik Demishtein et al.   JOURNAL OF MOLECULAR RECOGNITION

Abundance and Diversity of Dockerin-Containing Proteins in the Fiber-Degrading Rumen Bacterium, Ruminococcus flavefaciens FD-1

(2010) Marco T. Rincon et al.   PLoS One

Clostridium thermocellum cellulosomal genes are regulated by extracytoplasmic polysaccharides via alternative sigma factors

(2010) Y. Nataf et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Effect of Linker Length and Dockerin Position on Conversion of a Thermobifida fusca Endoglucanase to the Cellulosomal Mode

(2009) J. Caspi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Natural paradigms of plant cell wall degradation

(2009) Hui Wei et al.   CURRENT OPINION IN BIOTECHNOLOGY

Plant biomass degradation by gut microbiomes: more of the same or something new?

(2009) Mark Morrison et al.   CURRENT OPINION IN BIOTECHNOLOGY

The cellulosomes fromClostridium cellulolyticum

(2009) Imen Fendri et al.   FEBS Journal

The rosettazyme: A synthetic cellulosome

(2009) Shigenobu Mitsuzawa et al.   JOURNAL OF BIOTECHNOLOGY

Impact of Pretreated Switchgrass and Biomass Carbohydrates on Clostridium thermocellum ATCC 27405 Cellulosome Composition: A Quantitative Proteomic Analysis

(2009) Babu Raman et al.   PLoS One

Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases

(2009) Jennifer M. Brulc et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

On the remarkable mechanostability of scaffoldins and the mechanical clamp motif

(2009) A. Valbuena et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Modulation of cellulosome composition inClostridium cellulolyticum: Adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses

(2009) Jean-Charles Blouzard et al.   PROTEOMICS

Multivalent Anchoring and Oriented Display of Single-Domain Antibodies on Cellulose

(2009) Greg Hussack et al.   SENSORS

Noncellulosomal cohesin- and dockerin-like modules in the three domains of life

(2008) Ayelet Peer et al.   FEMS MICROBIOLOGY LETTERS

Mutations in the Scaffoldin Gene, cipA, of Clostridium thermocellum with Impaired Cellulosome Formation and Cellulose Hydrolysis: Insertions of a New Transposable Element, IS1447, and Implications for Cellulase Synergism on Crystalline Cellulose

(2008) V. V. Zverlov et al.   JOURNAL OF BACTERIOLOGY

Metagenomics

(2008) Philip Hugenholtz et al.   NATURE

Structural basis of Clostridium perfringens toxin complex formation

(2008) J. J. Adams et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cohesin-dockerin microarray: Diverse specificities between two complementary families of interacting protein modules

(2008) Rachel Haimovitz et al.   PROTEOMICS