Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific intracellular cytokine staining (ICS) and IFN-ɣ enzyme-linked ImmunoSpot (ELISpot) protocols

Influenza vaccines

(2022) Nadim Khalil et al.   CURRENT OPINION IN PEDIATRICS

The economic burden of influenza among adults aged 18 to 64: A systematic literature review

(2022) Caroline Courville et al.   Influenza and Other Respiratory Viruses

2020 White Paper on Recent Issues in Bioanalysis: Vaccine Assay Validation, qPCR Assay Validation, QC for CAR-T Flow Cytometry, NAb Assay Harmonization and ELISpot Validation (Part 3 – Recommendations on Immunogenicity Assay Strategies, NAb Assays, Biosimilars and FDA/EMA Immunogenicity Guidance/Guideline, Gene & Cell Therapy and Vaccine Assays)

(2021) Bart Corsaro et al.   Bioanalysis

Estimates of global seasonal influenza-associated respiratory mortality: a modelling study

(2018) A Danielle Iuliano et al.   LANCET

Effectiveness of influenza vaccines in preventing severe influenza illness among adults: A systematic review and meta-analysis of test-negative design case-control studies

(2017) Marc Rondy et al.   JOURNAL OF INFECTION

Data analysis as a source of variability of the HLA-peptide multimer assay: from manual gating to automated recognition of cell clusters

(2015) Cécile Gouttefangeas et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

SWIFT-scalable clustering for automated identification of rare cell populations in large, high-dimensional flow cytometry datasets, Part 2: Biological evaluation

(2014) Tim R. Mosmann et al.   CYTOMETRY PART A

The External Quality Assurance Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay

(2014) Ana M. Sanchez et al.   JOURNAL OF IMMUNOLOGICAL METHODS

A harmonized approach to intracellular cytokine staining gating: Results from an international multiconsortia proficiency panel conducted by the Cancer Immunotherapy Consortium (CIC/CRI)

(2013) Lisa K. McNeil et al.   CYTOMETRY PART A

Critical assessment of automated flow cytometry data analysis techniques

(2013) Nima Aghaeepour et al.   NATURE METHODS

Hierarchical Modeling for Rare Event Detection and Cell Subset Alignment across Flow Cytometry Samples

(2013) Andrew Cron et al.   PLoS Computational Biology

Harmonization of the intracellular cytokine staining assay

(2012) Marij J. P. Welters et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Harmonization of Immune Biomarker Assays for Clinical Studies

(2011) S. H. van der Burg et al.   Science Translational Medicine

Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: position statement of the T-Cell Workshop Committee of the Immunology of Diabetes Society

(2010) R. Mallone et al.   CLINICAL AND EXPERIMENTAL IMMUNOLOGY

Quality assurance of intracellular cytokine staining assays: Analysis of multiple rounds of proficiency testing

(2010) Maria C. Jaimes et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Equivalence of ELISpot Assays Demonstrated between Major HIV Network Laboratories

(2010) Dilbinder K. Gill et al.   PLoS One

Optimization and Limitations of Use of Cryopreserved Peripheral Blood Mononuclear Cells for Functional and Phenotypic T-Cell Characterization

(2009) A. Weinberg et al.   Clinical and Vaccine Immunology

ELISPOT assays provide reproducible results among different laboratories for T-cell immune monitoring—even in hands of ELISPOT-inexperienced investigators

(2009) W. Zhang et al.   Journal of Immunotoxicology

Concordant Proficiency in Measurement of T-Cell Immunity in Human Immunodeficiency Virus Vaccine Clinical Trials by Peripheral Blood Mononuclear Cell and Enzyme-Linked Immunospot Assays in Laboratories from Three Continents

(2008) M. J. Boaz et al.   Clinical and Vaccine Immunology

Statistical mixture modeling for cell subtype identification in flow cytometry

(2008) Cliburn Chan et al.   CYTOMETRY PART A