Data analysis as a source of variability of the HLA-peptide multimer assay: from manual gating to automated recognition of cell clusters

SWIFT-scalable clustering for automated identification of rare cell populations in large, high-dimensional flow cytometry datasets, Part 2: Biological evaluation

(2014) Tim R. Mosmann et al.   CYTOMETRY PART A

A harmonized approach to intracellular cytokine staining gating: Results from an international multiconsortia proficiency panel conducted by the Cancer Immunotherapy Consortium (CIC/CRI)

(2013) Lisa K. McNeil et al.   CYTOMETRY PART A

Critical assessment of automated flow cytometry data analysis techniques

(2013) Nima Aghaeepour et al.   NATURE METHODS

Hierarchical Modeling for Rare Event Detection and Cell Subset Alignment across Flow Cytometry Samples

(2013) Andrew Cron et al.   PLoS Computational Biology

Serum-free freezing media support high cell quality and excellent ELISPOT assay performance across a wide variety of different assay protocols

(2012) Helene Filbert et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Harmonization of the intracellular cytokine staining assay

(2012) Marij J. P. Welters et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Limited Induction of Tumor Cross-Reactive T Cells without a Measurable Clinical Benefit in Early Melanoma Patients Vaccinated with Human Leukocyte Antigen Class I-Modified Peptides

(2012) P. Filipazzi et al.   CLINICAL CANCER RESEARCH

T Cell Assays and MIATA: The Essential Minimum for Maximum Impact

(2012) C.M. Britten et al.   IMMUNITY

Multipeptide immune response to cancer vaccine IMA901 after single-dose cyclophosphamide associates with longer patient survival

(2012) Steffen Walter et al.   NATURE MEDICINE

Parallel detection of antigen-specific T cell responses by combinatorial encoding of MHC multimers

(2012) Rikke Sick Andersen et al.   Nature Protocols

Quality assurance for polychromatic flow cytometry using a suite of calibration beads

(2012) Stephen P Perfetto et al.   Nature Protocols

Exhaustion of tumor-specific CD8+ T cells in metastases from melanoma patients

(2011) Lukas Baitsch et al.   JOURNAL OF CLINICAL INVESTIGATION

A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols

(2011) Sebastian Attig et al.   Journal of Translational Medicine

Modeling flow cytometry data for cancer vaccine immune monitoring

(2010) Jacob Frelinger et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Serum is not required for ex vivo IFN-γ ELISPOT: a collaborative study of different protocols from the European CIMT Immunoguiding Program

(2010) A. Mander et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Publication of optimized multicolor immunofluorescence panels

(2010) Yolanda Mahnke et al.   CYTOMETRY PART A

Quality assurance of intracellular cytokine staining assays: Analysis of multiple rounds of proficiency testing

(2010) Maria C. Jaimes et al.   JOURNAL OF IMMUNOLOGICAL METHODS

A model for harmonizing flow cytometry in clinical trials

(2010) Holden T Maecker et al.   NATURE IMMUNOLOGY

Induction of complete and molecular remissions in acute myeloid leukemia by Wilms' tumor 1 antigen-targeted dendritic cell vaccination

(2010) V. F. Van Tendeloo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Harmonization guidelines for HLA-peptide multimer assays derived from results of a large scale international proficiency panel of the Cancer Vaccine Consortium

(2009) Cedrik Michael Britten et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Effect of Granulocyte/Macrophage Colony-Stimulating Factor on Circulating CD8+ and CD4+ T-Cell Responses to a Multipeptide Melanoma Vaccine: Outcome of a Multicenter Randomized Trial

(2009) C. L. Slingluff et al.   CLINICAL CANCER RESEARCH

Tricks with tetramers: how to get the most from multimeric peptide-MHC

(2009) Linda Wooldridge et al.   IMMUNOLOGY

Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+T cells with peptide-major histocompatibility complex class I tetramers

(2008) Pratip K. Chattopadhyay et al.   CYTOMETRY PART A

MIFlowCyt: The minimum information about a flow cytometry experiment

(2008) Jamie A. Lee et al.   CYTOMETRY PART A

Statistical mixture modeling for cell subtype identification in flow cytometry

(2008) Cliburn Chan et al.   CYTOMETRY PART A