Article
Chemistry, Multidisciplinary
Bram G. Soliman, Alessia Longoni, Mian Wang, Wanlu Li, Paulina N. Bernal, Alessandro Cianciosi, Gabriella C. J. Lindberg, Jos Malda, Juergen Groll, Tomasz Jungst, Riccardo Levato, Jelena Rnjak-Kovacina, Tim B. F. Woodfield, Yu Shrike Zhang, Khoon S. Lim
Summary: Sacrificial printing involves using 3D-printed sacrificial ink to create open-channels in hydrogel constructs. Traditional sacrificial inks lack the ability to mimic tissue development dynamics. To overcome this, a new class of sacrificial inks was developed with tailorable and programmable delayed dissolution profiles. These inks showed compatibility with various biofabrication technologies and allowed precise control over the introduction of architectural features into cell-laden hydrogel constructs.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Gabriel Grossbacher, Michael Bartolf-Kopp, Csaba Gergely, Paulina Nunez Bernal, Sammy Florczak, Mylene de Ruijter, Nuria Gines Rodriguez, Juergen Groll, Jos Malda, Tomasz Jungst, Riccardo Levato
Summary: The major challenge in biofabrication is capturing the complex composition of native tissues. Current 3D printing techniques have limited capacity to produce composite biomaterials. Volumetric bioprinting, a light-based technique, allows for the fabrication of 3D structures with enhanced design freedom, but the prints have low mechanical stability.
ADVANCED MATERIALS
(2023)
Review
Engineering, Biomedical
Martina Viola, Susanna Piluso, Jurgen Groll, Tina Vermonden, Jos Malda, Miguel Castilho
Summary: Biofabrication uses additive manufacturing techniques to create 3D structures that mimic physiological cellular environments and promote the growth of native tissues. Recent approaches integrate multiple technologies and materials of different length scales to enhance construct functionality. The review critically examines the interfaces between different material phases in fabricated structures, emphasizing their impact on the mechanical and biological properties of the engineered structures.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Engineering, Biomedical
Dishary Banerjee, Yogendra Pratap Singh, Pallab Datta, Veli Ozbolat, Aaron O'Donnell, Miji Yeo, Ibrahim T. Ozbolat
Summary: Biofabricated tissues have versatile applications in tissue engineering and regenerative medicine, as well as disease modeling and drug development and screening. Spheroid bioprinting has emerged as a crucial tool for assembling and organizing multiple types of cells into physiologically and histologically relevant tissues.
Article
Engineering, Biomedical
Margaret E. Prendergast, Jason A. Burdick
Summary: The study examines the characteristics of hydrogel inks in suspension bath printing through rheological, computational, and experimental analyses, emphasizing the importance of key rheological properties and print parameters in the printing process.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Materials Science, Multidisciplinary
Riccardo Rizzo, Dominic Ruetsche, Hao Liu, Parth Chansoria, Anny Wang, Amelia Hasenauer, Marcy Zenobi-Wong
Summary: Multiscale printing of 3D perfusable geometries is achieved by combining volumetric printing (VP) and high-resolution two-photon ablation (2PA), overcoming the challenge of generating freeform designs with features ranging from centimeter to micrometer scales. Optical tuning of the photoresin's refractive index is used to eliminate micrometer-size defects generated during VP, allowing for defect-free printing that can be combined with 2PA. A protein-based photoclick photoresin is introduced to meet the requirements of the 2PA process and facilitate VP. This hybrid strategy enables the generation of complex organotypic 3D vasculature-like constructs with multiscale features.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Engineering, Biomedical
Wei Long Ng, Teck Choon Ayi, Yi-Chun Liu, Swee Leong Sing, Wai Yee Yeong, Boon-Huan Tan
Summary: In this study, human triple-layered alveolar lung models were fabricated using the drop-on-demand (DOD) 3D bioprinting technique, which improved repeatability by achieving consistent cell output over time with different human alveolar cells. The 3D bioprinting platform offers an attractive tool for highly repeatable and scalable fabrication of 3D in-vitro human alveolar lung models.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2021)
Article
Chemistry, Multidisciplinary
Davide Ribezzi, Marieme Gueye, Sammy Florczak, Franziska Dusi, Dieuwke de Vos, Francesca Manente, Andreas Hierholzer, Martin Fussenegger, Massimiliano Caiazzo, Torsten Blunk, Jos Malda, Riccardo Levato
Summary: A novel technique called Embedded Extrusion-Volumetric Printing (EmVP) is introduced, combining extrusion bioprinting and layer-less, ultra-fast volumetric bioprinting. Light-responsive microgels are developed as bioresins for light-based volumetric bioprinting, providing a microporous environment for cell homing and self-organization. EmVP is used to create complex synthetic biology-inspired intercellular communication models, where adipocyte differentiation is regulated by optogenetic-engineered pancreatic cells.
ADVANCED MATERIALS
(2023)
Article
Biophysics
Chuang Gao, Chunxiang Lu, Zhian Jian, Tingrui Zhang, Zhongjian Chen, Quangang Zhu, Zongguang Tai, Yuanyuan Liu
Summary: The skin, as the first line of defense, is susceptible to damage and there is a growing demand for artificial skin in various fields. Traditional skin tissue engineering has made progress, but there are challenges and prospects in 3D bioprinting.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Engineering, Biomedical
Yanshen Yang, Yuanbo Jia, Qingzhen Yang, Feng Xu
Summary: Three-dimensional bioprinting is a promising approach to construct functional biomimetic tissues. Bio-inks, which are essential for cell microenvironment construction, are influenced by mechanical properties. With the advancement of functional biomaterials, engineered bio-inks have allowed the creation of cell mechanical microenvironments in vitro.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Review
Engineering, Biomedical
Kun Liu, Nan Hu, Zhihai Yu, Xinzhou Zhang, Hualin Ma, Huawei Qu, Changshun Ruan
Summary: This article summarizes the development of 3D printing and bioprinting technologies in urology over the past decade. By searching and summarizing the literature, eight common 3D printing technologies and their characteristics are introduced, and the application of 3D printing in urology, as well as the potential applications of 3D bioprinting, are discussed.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Chemistry, Multidisciplinary
Dayi Jeong, Jeong Wook Seo, Hong-Gu Lee, Woo Kyung Jung, Yong Ho Park, Hojae Bae
Summary: The interest in cultured meat is growing due to issues with conventional livestock industry. This study introduces 3D bioprinting for producing large cell aggregates for cultured meat production, and successfully creates scaffolds with living cells and large microchannels using bioinks.
Article
Chemistry, Multidisciplinary
Martina M. De Santis, Hani N. Alsafadi, Sinem Tas, Deniz A. Bolukbas, Sujeethkumar Prithiviraj, Iran A. N. Da Silva, Margareta Mittendorfer, Chiharu Ota, John Stegmayr, Fatima Daoud, Melanie Koenigshoff, Karl Sward, Jeffery A. Wood, Manlio Tassieri, Paul E. Bourgine, Sandra Lindstedt, Sofie Mohlin, Darcy E. Wagner
Summary: The study introduces a hybrid bioink composed of alginate and rECM for 3D bioprinting, showing promising printing properties and biological inductive capabilities for generating human tissue.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Malin Becker, Melvin Gurian, Maik Schot, Jeroen Leijten
Summary: Embedded 3D bioprinting using low-viscosity ink 3D (LoV3D) bioprinting based on aqueous two-phase stabilization allows for the fabrication of living constructs at high speeds with high viability. The liquid/liquid interfaces of LoV3D bioprinting offer unique advantages for fusing structures, creating vasculature, and modifying surfaces. The low interfacial tension of LoV3D bioprinting allows for nozzle-independent control over filament diameter, enabling the printing of a wide range of diameters down to the width of a single cell.
Review
Chemistry, Multidisciplinary
Sarah M. Hull, Lucia G. Brunel, Sarah C. Heilshorn
Summary: Using gel-phase materials as bioinks offers advantages in providing cell protection and biological signals. Challenges remain in terms of cell compatibility and biological functionality of bioink materials.
ADVANCED MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Samson Afewerki, Thiago Domingues Stocco, Andre Diniz Rosa da Silva, Andre Sales Aguiar Furtado, Gustavo Fernandes de Sousa, Guillermo U. Ruiz-Esparza, Thomas J. Webster, Fernanda R. Marciano, Maria Stromme, Yu Shrike Zhang, Anderson Oliveira Lobo
Summary: Precision medicine allows for tailored treatments, improving the efficiency and accuracy of disease prevention, diagnosis, and treatment. The combination of organ-on-a-chip and bioprinting can address precision medicine challenges and be utilized in the current COVID-19 pandemic for efficient diagnostics and treatments. This convergence enables advances in tools and in vitro models for personalized and faster screening of therapeutics.
MOLECULAR ASPECTS OF MEDICINE
(2023)
Article
Engineering, Biomedical
Liwen Zheng, Yaxian Liu, Lin Jiang, Xiaoping Wang, Yuqin Chen, Lan Li, Mingyu Song, Hongmei Zhang, Yu Shrike Zhang, Ximu Zhang
Summary: This study proposes a new strategy for pulp regeneration by using hydrogel microspheres incorporated with decellularized dental pulp matrix-derived bioactive factors to simulate a pulp-specific three-dimensional microenvironment. The constructed microspheres exhibit favorable plasticity, biocompatibility, and biological performances, and hDPSCs cultured on the microspheres demonstrate enhanced pulp-formation ability. In vivo experiments show that the microspheres facilitate the regeneration of pulp-like tissue and new dentin. The decellularized pulp matrix-derived bioactive factors mediate the multi-directional differentiation of hDPSCs to regenerate the pulp tissue.
ACTA BIOMATERIALIA
(2023)
Article
Materials Science, Multidisciplinary
Hossein Ravanbakhsh, Razyeh Behbahani, Hamidreza Yazdani Sarvestani, Elham Kiyani, Meysam Rahmat, Mikko Karttunen, Behnam Ashrafi
Summary: This study evaluates and assists the design of interlocked ceramics under thermal shock loading using finite-element and machine learning methods. The approach successfully finds the optimal designs among over 2 million cases.
ADVANCED ENGINEERING MATERIALS
(2023)
Review
Materials Science, Multidisciplinary
Xuan Mu, Marie Denise Gerhard-Herman, Yu Shrike Zhang
Summary: Blood vessel chips are bioengineered microdevices that mimic essential vascular structure and physiology, allowing for controlled microenvironments and spatial-temporal readouts. They provide promising opportunities to study vascular diseases and offer insights into biological processes and disease pathogenesis. The physiological relevance is crucial and they rely on bioinspired strategies and engineering approaches to replicate vascular physiology.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Review
Chemistry, Multidisciplinary
Carla Spatola Rossi, Frederic Coulon, Shaohua Ma, Yu Shrike Zhang, Zhugen Yang
Summary: Rapid, sensitive, and selective detection of live pathogens is crucial for quality control and risk assessment. Conventional methods are often complex, expensive, time-consuming, and not suitable for field testing or low-resource settings. Microfluidic devices that integrate laboratory functions in a miniaturized manner have emerged as a promising tool for rapid and sensitive pathogen detection.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Editorial Material
Engineering, Biomedical
Yu Shrike Zhang, Mario Moises Alvarez, Grissel Trujillo-de Santiago
Summary: The field of biofabrication has made significant advancements in the past decade, particularly in the generation of biomimetic models of human tissues to study healthy and diseased states. These models have broad applications in fundamental biology research and compound screening, including therapeutic agents. The United States FDA Modernization Act 2.0, which eliminates the requirement for animal tests before human drug trials, is expected to further accelerate the development of this field. This Special Issue emphasizes the latest developments in biofabrication for human disease modeling, including 3D printing, organ-on-a-chip, and their integration.
Review
Chemistry, Analytical
Manna Dai, Gao Xiao, Ming Shao, Yu Shrike Zhang
Summary: Organs-on-chips (OoCs) are advanced in vitro models that are miniature microfluidic systems. Deep learning, an emerging topic in machine learning, can extract hidden statistical relationships from input data. This review discusses the integration of deep learning with OoCs for drug screening and presents successful use cases. The future prospects, perspectives, and potential challenges of combining OoCs and deep learning for image processing and automation designs are also discussed.
Article
Chemistry, Multidisciplinary
Vahid Karamzadeh, Ahmad Sohrabi-Kashani, Molly Shen, David Juncker
Summary: This study introduces the digital manufacturing of functional microfluidic systems in the form of structurally encoded, self-powered capillaric circuits (CC) using new hydrophilic inks. Monolithic CCs are 3D printed with design innovations including circular cross-sections, embedded stop valves, and gyroid capillary pumps. Programmable liquid handling via retention burst valves and microfluidic chain reactions are shown and applied to an immunoassay.
ADVANCED MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Berivan Cecen, Shabir Hassan, Xin Li, Yu Shrike Zhang
Summary: Smart biomaterials that can alter their properties in response to external stimuli or environmental changes have gained significant attention in the biomedical community. Shape-memory materials, in particular, have been extensively studied for their potential biomedical applications, offering unique structural reconfiguration features and enhanced biocompatibility.
MACROMOLECULAR BIOSCIENCE
(2023)
Review
Engineering, Biomedical
Gang Ren, Xia Zhou, Ruimin Long, Maobin Xie, Ranjith Kumar Kankala, Shibin Wang, Yu Shrike Zhang, Yuangang Liu
Summary: In this review, the formation mechanism of magnetosomes and various modification methods are discussed. The biomedical advancements of bacterial magnetosomes in biomedical imaging, drug delivery, anticancer therapy, and biosensors are presented. Future applications and challenges are also discussed. This review summarizes the application of magnetosomes in the biomedical field, highlighting the latest advancements and exploring the future development.
BIOACTIVE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Philipp Weber, Ling Cai, Francisco Javier Aguilar Rojas, Carlos Ezio Garciamendez-Mijares, Maria Celeste Tirelli, Francesco Nalin, Jakub Jaroszewicz, Wojciech Swieszkowski, Marco Costantini, Yu Shrike Zhang
Summary: Three-dimensional (3D) printing has been successful in engineering human tissues, and the integration of a digital light processing (DLP) printing procedure with a microfluidic chip system allows for the production of size-tunable, 3D-printable porosities within a GelMA hydrogel matrix.
Article
Multidisciplinary Sciences
Yunhua Wang, Guoxia Zheng, Nan Jiang, Guoliang Ying, Yiwei Li, Xiaolu Cai, Jiashen Meng, Liqiang Mai, Ming Guo, Yu Shrike Zhang, Xingcai Zhang
Summary: This article discusses the importance of nature inspiration in micropatterned materials and presents the key principles, fabrication methods, and applications of nature-inspired micropatterns. By imitating natural strategies, engineering challenges can be solved and novel functions can be imparted to materials. Nature inspiration is an interdisciplinary field involving various materials and fabrication techniques.
NATURE REVIEWS METHODS PRIMERS
(2023)
Article
Multidisciplinary Sciences
Riccardo Levato, Oksana Dudaryeva, Carlos Ezio Garciamendez-Mijares, Bruce E. Kirkpatrick, Riccardo Rizzo, Jacob Schimelman, Kristi S. Anseth, Shaochen Chen, Marcy Zenobi-Wong, Yu Shrike Zhang
Summary: Light-based vat-polymerization bioprinting allows computer-aided patterning of 3D cell-laden structures using photoactivatable bioresin in vats. This technology, divided into stereolithography, digital light processing and volumetric additive manufacturing, has been widely developed and applied in biomedicine. This Primer introduces the methodology and variations of light-based vat-polymerization 3D bioprinting, as well as discusses key assessments and future directions of this method.
NATURE REVIEWS METHODS PRIMERS
(2023)
Article
Chemistry, Multidisciplinary
Xuan Li, Yin Liu, Li Li, Ran Huo, Farshid Ghezelbash, Zhenwei Ma, Guangyu Bao, Shiyu Liu, Zhen Yang, Michael H. Weber, Nicole Y. K. Li-Jessen, Lisbet Haglund, Jianyu Li
Summary: Introduction of a hybrid bioadhesive that can repair and regenerate intervertebral discs post-nucleotomy. The adhesive fills the cavity and the sealant fixes the defect. It exhibits strong adhesion and survives extreme loading. The adhesive can match the mechanical properties of the native nucleus pulposus, support cell viability and matrix deposition, and restore biomechanics of the discs.
MATERIALS HORIZONS
(2023)
Review
Chemistry, Multidisciplinary
Sajjad Rahmani Dabbagh, Misagh Rezapour Sarabi, Mehmet Tugrul Birtek, Nur Mustafaoglu, Yu Shrike Zhang, Savas Tasoglu
Summary: Organ-on-a-chip (OOC) platforms provide a more realistic environment compared to animal models and conventional cell cultures, offering continuous perfusion of cell cultures, integration of biosensors, and 3D structures fabricated through bioprinting. These platforms have great potential for understanding organ functions, studying disease impacts, and drug screening. This review presents an overview of bioprinting techniques, their advantages and limitations, recent advancements, applications, and future prospects of the 3D-bioprinted OOC platforms, as well as current challenges and future directions.
