Article
Optics
Jianan Fang, Kun Huang, E. Wu, Ming Yan, Heping Zeng
Summary: This article introduces an infrared 3D imaging system capable of detecting three-dimensional structure and reflectivity information. The system operates at single-photon sensitivity and femtosecond timing resolution, enabling imaging under low-light conditions. By nonlinear frequency upconversion and recording with a silicon camera, the presented system achieves high lateral and depth resolutions for 3D reconstruction. Additionally, a numerical denoiser based on spatiotemporal correlation allows the retrieval of object profile and reflectivity even with a detected flux below 0.05 photons/pixel/second. This infrared 3D imager features high detection sensitivity, precise timing resolution, and wide-field operation, offering new possibilities in life and material sciences.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Review
Engineering, Biomedical
Kun Liu, Nan Hu, Zhihai Yu, Xinzhou Zhang, Hualin Ma, Huawei Qu, Changshun Ruan
Summary: This article summarizes the development of 3D printing and bioprinting technologies in urology over the past decade. By searching and summarizing the literature, eight common 3D printing technologies and their characteristics are introduced, and the application of 3D printing in urology, as well as the potential applications of 3D bioprinting, are discussed.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Engineering, Manufacturing
Sophia N. Economidou, Md Jasim Uddin, Manuel J. Marques, Dennis Douroumis, Wan Ting Sow, Huaqiong Li, Andrew Reid, James F. C. Windmill, Adrian Podoleanu
Summary: The 3DMNMEMS is a novel drug delivery device that combines 3D printing, microneedles, and Microelectromechanical Systems (MEMS) to achieve versatile and controllable transdermal drug delivery. By utilizing advanced imaging techniques, the device's ability to monitor the distribution of liquid in skin tissue in real time is demonstrated, with in vivo testing showing improved glycemic control compared to subcutaneous injections. These results highlight the potential of the 3DMNMEMS as a universal personalized care transdermal drug delivery system.
ADDITIVE MANUFACTURING
(2021)
Article
Biotechnology & Applied Microbiology
Marta Merli, Lorenzo Sardelli, Nicolo Baranzini, Annalisa Grimaldi, Emanuela Jacchetti, Manuela Teresa Raimondi, Francesco Briatico-Vangosa, Paola Petrini, Marta Tunesi
Summary: This study proposes a highly controllable method to optimize the printability of internally crosslinked polysaccharides by introducing pH as a key parameter. The results indicate that pectin-based hydrogels could be successfully applied for neural cell culture.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Fatima Garcia-Villen, Amaia Guembe, Jose M. Rey, Teresa Zuniga, Sandra Ruiz-Alonso, Laura Saenz-del-Burgo, Jesus M. Izco, Jose I. Recalde, Jose Luis Pedraz
Summary: Collagen is a key protein for tissue engineering and 3D bioprinting, but it comes with challenges such as complex extraction processes and batch-to-batch reproducibility issues. This study evaluates the use of new, fibrillar type I collagen as a standardized and reproducible collagen source for 3D printing and bioprinting. The native, type I collagen demonstrated excellent performance during 3D printing and enabled cell inclusion without hindering printability.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Chemistry, Multidisciplinary
Daniel S. Reynolds, Irene de Lazaro, Manon L. Blache, Yutong Liu, Nicholas C. Jeffreys, Ramsey M. Doolittle, Estee Grandidier, Jason Olszewski, Mason T. Dacus, David J. Mooney, Jennifer A. Lewis
Summary: Embedded bioprinting using the new microporogen-structured (& mu;POROS) matrix allows for the rapid design and fabrication of complex tissues that mimic in vivo microenvironments. This matrix, composed of a gelatin-chitosan complex and a prepolymer collagen solution, enables good print fidelity while promoting cell viability, proliferation, and migration. The integration of the & mu;POROS matrix with embedded bioprinting opens new possibilities for drug discovery, disease modeling, and tissue engineering for therapeutic use.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Jiawen Li, Simon Thiele, Rodney W. Kirk, Bryden C. Quirk, Ayla Hoogendoorn, Yung Chih Chen, Karlheinz Peter, Stephen J. Nicholls, Johan W. Verjans, Peter J. Psaltis, Christina Bursill, Alois M. Herkommer, Harald Giessen, Robert A. McLaughlin
Summary: Multimodal microendoscopes enable simultaneous structural and molecular measurements in vivo, providing valuable insights into pathological changes associated with diseases. However, different optical imaging modalities have conflicting requirements for lens design, posing a challenge for the fabrication of miniaturized imaging probes. This study demonstrates an optical design using two-photon 3D printing to create a miniaturized lens that is optimized for both fluorescence and optical coherence tomography (OCT) imaging. The lens-in-lens design shows significant improvement in fluorescence sensitivity compared to conventional fiber-optic design approaches, and is successfully integrated into an intravascular catheter probe for simultaneous OCT and fluorescence imaging of a mouse artery in vivo.
Article
Polymer Science
Rory Gibney, Jennifer Patterson, Eleonora Ferraris
Summary: Research has successfully demonstrated the production of bioprinted recombinant collagen films suitable for corneal tissue engineering using aerosol jet printing technology, showing favorable mechanical and optical properties.
Article
Biochemical Research Methods
Zhijian Huang, Delang Hen, Zhichao Iang, Shuangyang Hang, Zhenyang Iu, Wufan Hen, Li Qi
Summary: This study proposes a fully automatic framework for 3D airway reconstruction based on endoscopic airway optical coherence tomography (OCT). It includes automatic detection of the airway lumen contour, correction of rotational distortion, and anatomic bending reconstruction. The proposed framework has the potential to improve the efficiency of endoscopic OCT image analysis.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Biochemical Research Methods
Zhijian Zhuang, Delang Chen, Zhichao Liang, Shuangyang Zhang, Zhenyang Liu, Wufan Chen, Li Qi
Summary: Endoscopic airway optical coherence tomography is a non-invasive and high resolution imaging technique used for the diagnosis and analysis of airway-related diseases. This study presents a fully automatic framework, using a long-range endoscopic OCT imaging system equipped with a magnetic tracker, to reconstruct the 3D upper airway model with correct bending anatomy.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Chemistry, Multidisciplinary
Davide Ribezzi, Marieme Gueye, Sammy Florczak, Franziska Dusi, Dieuwke de Vos, Francesca Manente, Andreas Hierholzer, Martin Fussenegger, Massimiliano Caiazzo, Torsten Blunk, Jos Malda, Riccardo Levato
Summary: A novel technique called Embedded Extrusion-Volumetric Printing (EmVP) is introduced, combining extrusion bioprinting and layer-less, ultra-fast volumetric bioprinting. Light-responsive microgels are developed as bioresins for light-based volumetric bioprinting, providing a microporous environment for cell homing and self-organization. EmVP is used to create complex synthetic biology-inspired intercellular communication models, where adipocyte differentiation is regulated by optogenetic-engineered pancreatic cells.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Sungchul Shin, Lucia G. Brunel, Betty Cai, David Kilian, Julien G. Roth, Alexis J. Seymour, Sarah C. Heilshorn
Summary: The GUIDE-3DP approach is proposed to construct perfusable networks with precise control over branching geometries and vessel sizes. It leverages the predictable diffusion of cross-linking reaction-initiators released from sacrificial inks to achieve user-specified channel dimensions. The versatility of GUIDE-3DP to be adapted for use with diverse physicochemical cross-linking mechanisms is demonstrated by designing seven printable material systems. This platform is uniquely suited for fabricating lumenized structures with complex shapes characteristic of multiple hollow vessels throughout the body.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Zhen Wang, Lei Xiang, Feng Lin, Yunkai Tang, Wenguo Cui
Summary: This review provides an overview of 3D bioprinting and the main strategies used for homeostasis bioprinting. It introduces the principles of 3D bioprinting system applied in homeostasis regulations and summarizes the specific strategies and potential trends using stimuli-response biomaterials. The recent breakthrough in vasculature fabrication shows a promising prospect in hormone regulation. Challenges and future prospects of homeostasis fabrication based on 3D bioprinting in regenerative medicine are also discussed.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Tamara Munoz-Ortiz, Idoia Alayeto, Jose Lifante, Dirk H. Ortgies, Riccardo Marin, Emma Martin Rodriguez, Maria del Carmen Iglesias de la Cruz, Gines Lifante-Pedrola, Jorge Rubio-Retama, Daniel Jaque
Summary: Nanothermometry utilizes nanoparticles as thermal probes, enabling remote and minimally invasive sensing. It has emerged as a powerful tool in biomedicine. However, the lack of 3D thermal imaging capability and readily available tools in clinic hinders its translation to the bedside.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Kun Peng Zhang, Yan Fei Liao, Bin Qiu, Yue Kun Zheng, Ling Ke Yu, Gong Han He, Qin Nan Chen, Dao Heng Sun
Summary: This research uses projection micro-stereolithography 3D printing technology and liquid metal filling method to manufacture conformal and 3D metamaterials, improving transmission properties of the metamaterial devices by optimizing filling channel layout and surface post-treatment process.
Article
Materials Science, Biomaterials
Wenhuan Sun, Joshua W. Tashman, Daniel J. Shiwarski, Adam W. Feinberg, Victoria A. Webster-Wood
Summary: This study demonstrates long-fiber embedded hydrogel 3D printing using a multihead printing platform, achieving significant structural reinforcement. The technique is cost-effective, compatible with open-source software and hardware, and easily adaptable for new research applications.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Daniel J. Shiwarski, Andrew Hudson, Joshua Tashman, Adam Straub, Adam Feinberg
Article
Engineering, Biomedical
Jacqueline Bliley, Joshua Tashman, Maria Stang, Brian Coffin, Daniel Shiwarski, Andrew Lee, Thomas Hinton, Adam Feinberg
Summary: This study demonstrates the 3D bioprinting of a simplified heart model resembling embryonic development. The engineered heart tubes were cellularized and showed synchronous contractions similar to natural heart beats. These findings establish the feasibility of creating 3D bioprinted human heart tubes and open the door for engineering more complex heart muscle structures.
Article
Multidisciplinary Sciences
Kuanren Qian, Aishwarya Pawar, Ashlee Liao, Cosmin Anitescu, Victoria Webster-Wood, Adam W. Feinberg, Timon Rabczuk, Yongjie Jessica Zhang
Summary: We present a new computational framework for neuron growth based on the phase field method, and develop a software package called NeuronGrowth_IGAcollocation. By considering the effect of tubulin, we simulate different stages of neuron growth, including lamellipodia formation, initial neurite outgrowth, axon differentiation, and dendrite formation. The simulation results qualitatively and quantitatively reproduce neuron morphologies and can be extended to the formation of neurite networks.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Jennifer Cable, Paola Arlotta, Kevin Kit Parker, Alex J. Hughes, Katharine Goodwin, Christine L. Mummery, Roger D. Kamm, Sandra J. Engle, Danilo A. Tagle, Sylvia F. Boj, Alice E. Stanton, Yoshihiro Morishita, Melissa L. Kemp, Dennis A. Norfleet, Elebeoba E. May, Aric Lu, Rashid Bashir, Adam W. Feinberg, Sarah M. Hull, Anjelica L. Gonzalez, Michael R. Blatchley, Nuria Montserrat Pulido, Ryuji Morizane, Todd C. McDevitt, Deepak Mishra, Adriana Mulero-Russe
Summary: The ability to engineer complex multicellular systems has great potential for understanding biology and disease, and improving drug development. Engineering living systems requires a detailed understanding of the signals between cells and their environment, and recent advances have focused on cell cooperation, organ-on-a-chip models, biological robots, and organoids.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Materials Science, Multidisciplinary
Maria Stang, Joshua Tashman, Daniel Shiwarski, Humphrey Yang, Lining Yao, Adam Feinberg
Summary: Traditional 3D printing methods are not effective for thermally-cured thermoset elastomers due to their viscosity, gelation kinetics, and surface tension. In this study, the researchers achieved complex 3D printing of thermoset elastomers by modifying the rheological properties of the material and implementing print process controls, opening up new possibilities for the application of these materials.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Multidisciplinary Sciences
Joshua W. Tashman, Daniel J. Shiwarski, Adam W. Feinberg
Summary: The application of 3D printing to biological research has provided a new method for organizing cells and biological materials into complex 3D structures. This study converts a low-cost 3D printer into a bioprinter using affordable components, achieving high accuracy and fidelity in bioprinting. The components and instructions are provided as open-source 3D models, contributing to the accessibility and customization of bioprinting technology.
SCIENTIFIC REPORTS
(2022)
Article
Materials Science, Multidisciplinary
Neeha Dev Arun, Humphrey Yang, Lining Yao, Adam W. W. Feinberg
Summary: Thermally cured thermoset polymers, such as epoxies, are commonly used in industry due to their excellent thermal, chemical, and electrical resistance, as well as their mechanical properties. However, 3D printing these materials without rheological modification can be challenging as they tend to flow and lose shape during extrusion. A solution to this is 3D printing inside a support bath, which allows the liquid polymer to be held in place until fully cured. In this study, Freeform Reversible Embedding (FRE) is used to 3D print off-the-shelf thermoset epoxy into lattice structures using nonplanar extrusion, demonstrating the advantages of this approach.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Multidisciplinary
Wenhuan Sun, Avery S. Williamson, Ravesh Sukhnandan, Carmel Majidi, Lining Yao, Adam W. Feinberg, Victoria A. Webster-Wood
Summary: This study utilizes 3D printing technology to fabricate biodegradable and digestible marine hydraulic actuators using printable thin-wall structures. Sustainable biomaterials sourced from brown seaweed, such as calcium-alginate hydrogels, are used to achieve complex shapes and internal cavities in the components. The structural stiffness and morphology of the alginate actuators are dynamically modified through a reversible chelation-crosslinking mechanism. This research improves the manufacturing process for complex soft devices and expands the design space for biodegradable marine robots using biologically-sourced materials.
ADVANCED FUNCTIONAL MATERIALS
(2023)