Article
Chemistry, Analytical
Takeru Yamazaki, Xiao Liu, Young-Tae Chang, Satoshi Arai
Summary: Fluorescence thermometry is a microscopy technique that records temperature changes through fluorescence signals. We developed small-molecule thermometers for quantitative temperature mapping using fluorescence lifetime imaging (FLIM). However, due to expensive instrumentation, we investigated the applicability and limitations of fluorescence intensity (FI) analysis for thermometry. Comparing FI and FLIM-based thermometry, we concluded that FI-based thermometry can be used qualitatively if there are no critical shape changes or dye leakage from the target organelle.
Article
Chemistry, Multidisciplinary
Xuan Liu, Jian Hou, Jingmei Ou, Manling Yan
Summary: This study reports a series of novel tridurylboron-thermoplastic polyurethane (TPU) polymer ratiometric fluorescence thermometers. From -10 degrees to 60 degrees C, the PPB1-TPU system exhibits ultra-sensitive fluorescence ratio changes, with an absolute sensitivity of 14.5% degrees C-1 (40 degrees C) and a relative sensitivity of 6.3% degrees C-1 (35 degrees C). There are distinct fluorescence color changes of the PPB1-TPU film placed in saltwater of different temperatures, demonstrating great potential for application.
Article
Materials Science, Ceramics
Qianchao Ma, Qiyun Liu, Meihua Wu, Yifei Liu, Rui Wang, Ruxian Zhou, Yanheng Xu, Haichao Wei, Ruiyu Mi, Xin Min, Lefu Mei, Zhaohui Huang, Bin Ma
Summary: In this study, Eu3+ doped La2(MoO4)3 material was synthesized via the high-temperature solid-state method. The phase composition was confirmed by XRD analysis and the microstructure was observed through SEM. The fluorescence properties of Eu3+ in the doped samples were investigated and the prepared phosphors were found to be color tunable. The results imply that Eu3+-doped La2(MoO4)3 is a promising material for high performance non-contact optical thermometers.
CERAMICS INTERNATIONAL
(2023)
Article
Chemistry, Multidisciplinary
Chuanhao Liu, Lin Zhou, Ying Zheng, Huizi Man, Zhiwei Ye, Xinfu Zhang, Lijuan Xie, Yi Xiao
Summary: We proposed to monitor the early stage of alcohol-induced liver injury by quantitatively detecting Golgi viscosity. A Golgi-targeted fluorescent rotor (GA-Vis) was developed for this purpose. Changes in Golgi viscosity during alcohol-induced liver injury were quantitatively evaluated using GA-Vis through fluorescence lifetime imaging in live cells and zebrafish. GA-Vis was qualified as a practical tool for future diagnoses of alcohol-induced liver injury.
CHEMICAL COMMUNICATIONS
(2022)
Article
Optics
Weidi Xia, Li Li, Peixin Yang, Faling Ling, Yongjie Wang, Zhongmin Cao, Sha Jiang, Guotao Xiang, Xianju Zhou, Yongbin Hua
Summary: A series of single and co-doped Sr8MgLa(PO4)(7) phosphors with Tb3+ and Eu3+ ions were successfully prepared, showing color-tunable performances and introducing dual-model optical thermometer techniques. The energy transfer mechanism between Tb3+ and Eu3+ was determined, and the sensing sensitivity was investigated, demonstrating the potential for dual-model thermometers.
JOURNAL OF LUMINESCENCE
(2021)
Article
Chemistry, Multidisciplinary
Elvin Salerno, Albano N. Carneiro Neto, Svetlana Eliseeva, Miguel A. Hernandez-Rodriguez, Jacob C. Lutter, Timothee Lathion, Jeff W. Kampf, Stephane Petoud, Luis D. Carlos, Vincent L. Pecoraro
Summary: This study examines the impact of ligands' energy levels on the thermal dependence of lanthanide emission and successfully creates new molecular nanothermometers. The results show that the difference in energy levels between the triplet state and the excited state of lanthanides is a key factor controlling the thermal dependence of emission intensity. Moreover, the energy gap between ligand-centered states also affects the thermal behavior.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Nicholas Banahene, Dana M. Gepford, Kyle J. Biegas, Daniel H. Swanson, Yen-Pang Hsu, Brennan A. Murphy, Zachary E. Taylor, Irene Lepori, M. Sloan Siegrist, Andres Obregon-Henao, Michael S. Van Nieuwenhze, Benjamin M. Swarts
Summary: This study presents a trehalose-based fluorogenic probe, RMR-Tre, which exploits the enzymes and environment of the mycobacterial outer membrane to achieve fluorescence activation. RMR-Tre enables fast, no-wash, low-background fluorescence detection of live mycobacteria. Compared to existing probes, RMR-Tre exhibits a 100-fold enhancement in M. tuberculosis labeling and has the potential for TB diagnostic tool.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Nanoscience & Nanotechnology
Anqi Zhang, Zhen Sun, Zhiying Wang, Mochen Jia, Byung Chun Choi, Zuoling Fu, Jung Hyun Jeong, Sung Heum Park
Summary: A double-perovskite Ca2LaNbO6:Pr3+ color tunable phosphor was synthesized for optical temperature sensing and anti-counterfeiting applications, showing potential versatility.
SCRIPTA MATERIALIA
(2022)
Article
Chemistry, Applied
Jingxue Zhang, Lefu Mei, Yuanyuan Zhang, Qingfeng Guo, Libing Liao, Haikun Liu
Summary: Tb3+, Dy3+-co-doped phosphors were prepared and their potential application in optical temperature measurements was investigated. Energy transfer between Dy3+ and Tb3+ was confirmed, and the fluorescence intensity showed a trend of first increasing and then decreasing with the increase of Dy3+ concentration.
JOURNAL OF RARE EARTHS
(2021)
Article
Chemistry, Multidisciplinary
Y. Hua, T. Wang, W. Xia, J. S. Yu, L. Li
Summary: Novel red-emitting Ba2Y0.8Eu0.2NbO6:Mn4+ phosphors were designed for multi-type non-contact luminescent thermometers based on the dual-emission states and temperature-dependent lifetime models. The phosphors showed high sensing sensitivities and have the potential for various applications in thermometers.
MATERIALS TODAY CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Aurimas Vysniauskas, Bethan Cornell, Peter S. Sherin, Karolina Maleckaite, Marketa Kubankova, Maria Angeles Izquierdo, Thanh Truc Vu, Yulia A. Volkova, Ekaterina M. Budynina, Carla Molteni, Marina K. Kuimova
Summary: A new cell-permeable fluorescence probe based on BODIPY was developed to respond to environmental temperature changes, with reduced viscosity dependence through functionalization with cyclopropyl substituents. This probe shows potential for applications in fluorescence lifetime imaging microscopy (FLIM) due to its temperature-dependent fluorescence decays.
Article
Materials Science, Multidisciplinary
Benedikt Bendel, Markus Suta
Summary: Luminescent thermometers are potential candidates for remote and non-invasive temperature sensing. Boltzmann thermometers rely on thermal coupling between two excited levels, while other luminescent thermometers rely on interconfigurational crossover between excited state potentials. Guidelines for fitting models and verifying their physical reliability are currently lacking for this class of thermometers.
JOURNAL OF MATERIALS CHEMISTRY C
(2022)
Article
Physics, Applied
Serhii Volosheniuk, Damian Bouwmeester, Chunwei Hsu, H. S. J. van der Zant, Pascal Gehring
Summary: Thermocurrent flowing through a single-molecule device provides valuable information about its quantum properties, electronic and phononic excitation spectra, and entropy. Experimentally measuring the thermoelectric heat-to-charge conversion efficiency can help select suitable molecules for highly efficient energy conversion devices predicted by theoretical studies. A superconductor-normal metal-superconductor Josephson junction thermometer is incorporated into the single-molecule device to accurately determine the temperature bias. This device architecture enables precise thermoelectric experiments on individual molecules at millikelvin temperatures.
APPLIED PHYSICS LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Sofia Zanella, Maxime Aragon-Alberti, Carlos D. S. Brite, Fabrice Salles, Luis D. Carlos, Jerome Long
Summary: Luminescent thermometry enables remote temperature detection and has great potential in future technological applications. We demonstrate for the first time that luminescence thermometry can be combined with a complementary temperature readout related to a different property. By taking advantage of the temperature dependence of magnetic and luminescence features in Single-Molecule Magnets (SMM), we develop original dual magneto-optical molecular thermometers that offer a 10-fold improvement in relative thermal sensitivity over the whole temperature range.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Inorganic & Nuclear
Ling Li, Ji-Yong Zou, Chao Hong, Sheng-Yong You, Li Zhang
Summary: Developing a convenient and accurate temperature detection method is crucial in various fields such as industrial production, biochemical processes, and scientific research. In this study, two zinc-based metal-organic frameworks (MOFs) were synthesized as luminescence thermometers for tunable temperature range. The results showed that the luminescence signals of the MOFs decreased with increasing temperature, allowing for non-destructive temperature detection. Additionally, a linear relationship was observed between the luminescence intensities and low temperatures, indicating high relative sensitivity and small temperature uncertainties.
JOURNAL OF SOLID STATE CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Hong-Guen Lee, Jin Hae Kim, Tumpa Gorai, Young Ho Ko, Haw-Young Kwon, Wooseong Chung, Ilha Hwang, Sungsu Lim, Yun Kyung Kim, Kwanwoo Shin, Young-Tae Chang, Kimoon Kim, Kyeng Min Park
Summary: This study presents a new supramolecular approach using a synthetic cell system to control the initiation of protein aggregation and mimic various conditions of lipid membranes. The approach enables systematic investigations of membrane-dependent effects on protein aggregation by visualization. The concurrent use of synthetic cells and natural cells demonstrates the potential of this approach for studying inter- and intracellularly transmittable protein aggregation.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Min Gao, Sun Hyeok Lee, Raj Kumar Das, Haw-Young Kwon, Heon Seok Kim, Young-Tae Chang
Summary: The development of a live B-cell selective small molecule probe CDyB has been achieved through unbiased fluorescence library screening. The molecular target of CDyB is identified as the SLC35C2 transporter. The fluorescence of CDyB and the expression of SLC35C2 are positively correlated with B-cell maturation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Immunology
Seon-Young Park, HyeJin Yang, Minsook Ye, Xiao Liu, Insop Shim, Young-Tae Chang, Hyunsu Bae
Summary: This study found that adoptive transfer of Tregs can improve neurodegeneration in TMT-intoxicated mice by promoting neurogenesis and modulating microglial activation and polarization.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Biotechnology & Applied Microbiology
Keun-Tae Kim, Ji-Young Oh, Seokwoo Park, Seong-Min Kim, Patterson Benjamin, In-Hyun Park, Kwang-Hoon Chun, Young-Tae Chang, Hyuk-Jin Cha
Summary: The study investigates the distinct traits between naive and primed pluripotent stem cells, revealing the high intracellular glycogen levels and requirement for oxidative phosphorylation in naive cells. Naive cells can be rescued by active oxidative phosphorylation and high levels of intracellular glycogen, providing a potential novel biomarker for metabolic remodeling during the transition of naive pluripotency.
METABOLIC ENGINEERING
(2022)
Review
Chemistry, Multidisciplinary
Yang Zhong, Xueping Zeng, Yuling Zeng, Lulu Yang, Juanjuan Peng, Lingzhi Zhao, Young-tae Chang
Summary: Cardiovascular diseases, including atherosclerosis, thrombosis, myocardial/cerebral infarction, and ischemia-reperfusion injury, are the leading cause of disability and death globally. The development of nanotechnology has provided opportunities for the precise delivery of active agents for the diagnosis and therapy of these diseases. This article summarizes the design strategies and significance of engineered nanomaterials in the diagnosis and treatment of diverse cardiovascular diseases, and prospects the challenges and research targets in this field.
Article
Chemistry, Analytical
Xiao Liu, Eun Gyeong Choi, Songbin Cui, Kapil Kumar, Myongji Cho, Young-Tae Chang
Summary: The article introduces a new fluorescent molecular probe BOS for detecting bad cooking oil, which detects bad oil through monitoring viscosity and pH changes, and constructs a portable platform BOSS for quick and easy field detection of bad oil.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Hong-Guen Lee, Avinash Dhamija, Chandan K. Das, Kyeng Min Park, Young-Tae Chang, Lars V. Schaefer, Kimoon Kim
Summary: This study presents synthetic monosaccharide channels based on shape-persistent organic cages, porphyrin boxes (PBs), for facile transmembrane transport of glucose and fructose. PBs have a higher transport rate for glucose and fructose compared to disaccharides, demonstrated by enzyme assays and molecular dynamics simulations. The transport rate can be controlled by changing the length of the alkyl chains on the cage windows. When the natural glucose transporter GLUT1 is blocked, PBs are able to transport glucose across living-cell membranes and enhance cell viability. Time-dependent live-cell imaging and MTT assays confirm the cyto-compatibility of PBs. The monosaccharide-selective transport ability of PBs resembles that of natural glucose transporters (GLUTs), which play critical roles in biological functions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Applied
Thanawat Chaiprasert, Supphachok Chanmungkalakul, Yujia Liu, Teeraya Bureerug, Komthep Silpcharu, Masafumi Unno, Liu Xiaogang, Vuthichai Ervithayasuporn, Young-Tae Chang, Paitoon Rashatasakhon
Summary: Two new all-cis pyrene-substituted cyclic tetrasiloxanes (JR-01 and JR-02) have been synthesized and studied as selective metal ion sensors. Both compounds exhibit solvent-and concentration-dependent excimer fluorescence emission at 482 nm. They also show highly selective fluorescence quenching by Au(III) ion with detection limits of 0.9 and 0.8 mu M for JR-01 and JR-02, respectively. Data from various experiments indicate a plausible mechanism involving the formation of a non-emissive Au(III) complex.
Article
Chemistry, Multidisciplinary
Heewon Cho, Haw-Young Kwon, Sun Hyeok Lee, Hong-Guen Lee, Nam-Young Kang, Young-Tae Chang
Summary: Macrophages are highly adaptable immune cells that can change their characteristics in response to the environment. They can be broadly categorized into M1 and M2 subsets, with M1 macrophages showing aggressive behavior against invaders while M2 macrophages exhibit anti-inflammatory properties and potentially assist in tumor immunity. However, monitoring M2 macrophages in real time has been challenging due to the lack of suitable detection tools. In this study, researchers developed a novel M2 selective probe, CDg18, which utilizes M2-favored fatty acid transporters for live-cell distinction. By combining this probe with an M1 probe, CDr17, the researchers were able to monitor the dynamic phenotypic changes of macrophages during reprogramming, allowing for simultaneous real-time monitoring of the diminishing M2 characteristics and emerging M1 markers.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Mousumi Baruah, Haw-Young Kwon, Heewon Cho, Young-Tae Chang, Animesh Samanta
Summary: The differentiation of macrophage phenotypes is crucial for accurate diagnosis and treatment of inflammatory diseases. A fluorescent probe called PMS-T has been developed to distinguish M1 from M0 and M2 macrophages by detecting the levels of hypochlorite (OCl-). This probe has the potential to serve as a chemical tool for monitoring inflammatory diseases, such as rheumatoid arthritis, and overcome the limitations of immunohistochemistry.
ANALYTICAL CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Tusar Kanta Acharya, Shamit Kumar, Tejas Pravin Rokade, Young-Tae Chang, Chandan Goswami
Summary: The activation of T cells is influenced by temperature and intracellular calcium signaling. TRPV4, a non-selective calcium channel, is expressed in T cells and located in their mitochondria. Activation of TRPV4 increases mitochondrial calcium levels and affects mitochondrial temperature and metabolism.
Article
Chemistry, Analytical
Seong-Hyeon Park, Hong-Guen Lee, Xiao Liu, Sung Kwang Lee, Young-Tae Chang
Summary: Researchers have developed a multi-organelle prediction model that can accurately predict the destination of fluorescent probes among nine organelles. The model, trained using the Random Forest algorithm, achieved a prediction accuracy of 75% for the target organelles of fluorescent probes. A total of 38 key parameters, including LogD, pKa, hydrophilic-lipophilic balance (HLB), and topological polar surface area (TPSA), have been identified to significantly impact the organelle selectivity of the probes.
Article
Chemistry, Analytical
Songhui Kim, Masahiro Fukuda, Jung Yeol Lee, Young-Tae Chang, H. Shawn Je, Beomsue Kim
Summary: CDr20 is a small-molecule fluorescent chemosensor that can track immune cells expressing UGT1A7C activity and selectively label monocytes/macrophages and neutrophils. It can be exported from cells through a glucuronidation reaction and differentiate cell types within blood.
Article
Chemistry, Analytical
Takeru Yamazaki, Xiao Liu, Young-Tae Chang, Satoshi Arai
Summary: Fluorescence thermometry is a microscopy technique that records temperature changes through fluorescence signals. We developed small-molecule thermometers for quantitative temperature mapping using fluorescence lifetime imaging (FLIM). However, due to expensive instrumentation, we investigated the applicability and limitations of fluorescence intensity (FI) analysis for thermometry. Comparing FI and FLIM-based thermometry, we concluded that FI-based thermometry can be used qualitatively if there are no critical shape changes or dye leakage from the target organelle.
Article
Radiology, Nuclear Medicine & Medical Imaging
Seul-Ki Mun, Hyun Bo Sim, Ji Yeon Han, Hyeongyeong Kim, Dae-Han Park, Dong-Jo Chang, Sung-Tae Yee, Young-Tae Chang, Jong-Jin Kim
Summary: The development of efficient biomarkers and probes for monitoring and treating cancer, specifically metastatic cancer, is crucial for patient outcomes and drug discovery.