期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 62, 期 2, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202213563
关键词
Fluorescence; Molecular Rotor; Mycobacteria; Trehalose; Tuberculosis
This study presents a trehalose-based fluorogenic probe, RMR-Tre, which exploits the enzymes and environment of the mycobacterial outer membrane to achieve fluorescence activation. RMR-Tre enables fast, no-wash, low-background fluorescence detection of live mycobacteria. Compared to existing probes, RMR-Tre exhibits a 100-fold enhancement in M. tuberculosis labeling and has the potential for TB diagnostic tool.
Increasing the speed, specificity, sensitivity, and accessibility of mycobacteria detection tools are important challenges for tuberculosis (TB) research and diagnosis. In this regard, previously reported fluorogenic trehalose analogues have shown potential, but their green-emitting dyes may limit sensitivity and applications in complex settings. Here, we describe a trehalose-based fluorogenic probe featuring a molecular rotor turn-on fluorophore with bright far-red emission (RMR-Tre). RMR-Tre, which exploits the unique biosynthetic enzymes and environment of the mycobacterial outer membrane to achieve fluorescence activation, enables fast, no-wash, low-background fluorescence detection of live mycobacteria. Aided by the red-shifted molecular rotor fluorophore, RMR-Tre exhibited up to a 100-fold enhancement in M. tuberculosis labeling compared to existing fluorogenic trehalose probes. We show that RMR-Tre reports on M. tuberculosis drug resistance in a facile assay, demonstrating its potential as a TB diagnostic tool.
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