Automated, scaled, transposon-based production of CAR T cells

The Past, Present, and Future of Non-Viral CAR T Cells

(2022) Alex Moretti et al.   Frontiers in Immunology

Investigation of product derived lymphoma following infusion of piggyBac modified CD19 chimeric antigen receptor T-cells

(2021) Kenneth Paul Micklethwaite et al.   BLOOD

CARAMBA: a first-in-human clinical trial with SLAMF7 CAR-T cells prepared by virus-free Sleeping Beauty gene transfer to treat multiple myeloma

(2021) Sabrina Prommersberger et al.   GENE THERAPY

Automated production of CCR5-negative CD4+-T cells in a GMP-compatible, clinical scale for treatment of HIV-positive patients

(2021) Lea Isabell Schwarze et al.   GENE THERAPY

Production and Application of CAR T Cells: Current and Future Role of Europe

(2021) Vladan Vucinic et al.   Frontiers in Medicine

Current Limitations and Perspectives of Chimeric Antigen Receptor-T-Cells in Acute Myeloid Leukemia

(2021) Marius Maucher et al.   Cancers

Cost of decentralized CAR T‐cell production in an academic nonprofit setting

(2020) Tao Ran et al.   INTERNATIONAL JOURNAL OF CANCER

Automated Manufacture of Autologous CD19 CAR-T Cells for Treatment of Non-hodgkin Lymphoma

(2020) Zachary Jackson et al.   Frontiers in Immunology

Latest Advances for the Sleeping Beauty Transposon System: 23 Years of Insomnia but Prettier than Ever

(2020) Maximilian Amberger et al.   BIOESSAYS

Lisocabtagene maraleucel for patients with relapsed or refractory large B-cell lymphomas (TRANSCEND NHL 001): a multicentre seamless design study

(2020) Jeremy S Abramson et al.   LANCET

Automated generation of gene-edited CAR T cells at clinical scale

(2020) Jamal Alzubi et al.   Molecular Therapy-Methods & Clinical Development

Closed-system manufacturing of CD19 and dual-targeted CD20/19 chimeric antigen receptor T cells using the CliniMACS Prodigy device at an academic medical center

(2018) Fenlu Zhu et al.   CYTOTHERAPY

Efficient Non-Viral T-Cell Engineering by Sleeping Beauty Minicircles Diminishing DNA Toxicity and miRNAs Silencing the Endogenous T-Cell Receptors

(2018) Julian Clauss et al.   HUMAN GENE THERAPY

Efficient Non-viral Gene Delivery into Human Hematopoietic Stem Cells by Minicircle Sleeping Beauty Transposon Vectors

(2018) Marta Holstein et al.   MOLECULAR THERAPY

Determinants of response and resistance to CD19 chimeric antigen receptor (CAR) T cell therapy of chronic lymphocytic leukemia

(2018) Joseph A. Fraietta et al.   NATURE MEDICINE

Automated Manufacturing of Potent CD20-Directed Chimeric Antigen Receptor T Cells for Clinical Use

(2017) Dominik Lock et al.   HUMAN GENE THERAPY

Precision Tumor Recognition by T Cells With Combinatorial Antigen-Sensing Circuits

(2016) Kole T. Roybal et al.   CELL

Automated manufacturing of chimeric antigen receptor T cells for adoptive immunotherapy using CliniMACS Prodigy

(2016) Ulrike Mock et al.   CYTOTHERAPY

Automated Enrichment, Transduction, and Expansion of Clinical-Scale CD62L+T Cells for Manufacturing of Gene Therapy Medicinal Products

(2016) Christoph Priesner et al.   HUMAN GENE THERAPY

CD19 CAR–T cells of defined CD4+:CD8+ composition in adult B cell ALL patients

(2016) Cameron J. Turtle et al.   JOURNAL OF CLINICAL INVESTIGATION

Phase I trials using Sleeping Beauty to generate CD19-specific CAR T cells

(2016) Partow Kebriaei et al.   JOURNAL OF CLINICAL INVESTIGATION

Enhanced CAR T-cell engineering using non-viral Sleeping Beauty transposition from minicircle vectors

(2016) R Monjezi et al.   LEUKEMIA

Genome-wide Profiling Reveals Remarkable Parallels Between Insertion Site Selection Properties of the MLV Retrovirus and the piggyBac Transposon in Primary Human CD4+ T Cells

(2016) Andreas Gogol-Döring et al.   MOLECULAR THERAPY

Towards a commercial process for the manufacture of genetically modified T cells for therapy

(2015) A D Kaiser et al.   CANCER GENE THERAPY

Chimeric antigen receptor-modified T cells derived from defined CD8+ and CD4+ subsets confer superior antitumor reactivity in vivo

(2015) D Sommermeyer et al.   LEUKEMIA

The Nonsignaling Extracellular Spacer Domain of Chimeric Antigen Receptors Is Decisive for In Vivo Antitumor Activity

(2014) M. Hudecek et al.   Cancer Immunology Research

Integrated Clinical Scale Manufacturing System for Cellular Products Derived by Magnetic Cell Separation, Centrifugation and Cell Culture

(2013) Michael Apel et al.   CHEMIE INGENIEUR TECHNIK

IL-7 and IL-15 instruct the generation of human memory stem T cells from naive precursors

(2012) N. Cieri et al.   BLOOD

A transgene-encoded cell surface polypeptide for selection, in vivo tracking, and ablation of engineered cells

(2011) X. Wang et al.   BLOOD

Analysis of Lentiviral Vector Integration in HIV+ Study Subjects Receiving Autologous Infusions of Gene Modified CD4+ T Cells

(2009) Gary P Wang et al.   MOLECULAR THERAPY

Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates

(2009) Lajos Mátés et al.   NATURE GENETICS