Enhanced CAR T-cell engineering using non-viral Sleeping Beauty transposition from minicircle vectors

CD19 CAR–T cells of defined CD4+:CD8+ composition in adult B cell ALL patients

(2016) Cameron J. Turtle et al.   JOURNAL OF CLINICAL INVESTIGATION

Gene Insertion Into Genomic Safe Harbors for Human Gene Therapy

(2016) Eirini P Papapetrou et al.   MOLECULAR THERAPY

Chemotherapy-Refractory Diffuse Large B-Cell Lymphoma and Indolent B-Cell Malignancies Can Be Effectively Treated With Autologous T Cells Expressing an Anti-CD19 Chimeric Antigen Receptor

(2015) James N. Kochenderfer et al.   JOURNAL OF CLINICAL ONCOLOGY

Adoptive cellular therapy: A race to the finish line

(2015) Carl H. June et al.   Science Translational Medicine

Identification of Chimeric Antigen Receptors That Mediate Constitutive or Inducible Proliferation of T Cells

(2015) M. J. Frigault et al.   Cancer Immunology Research

CD19-CAR Trials

(2014) Carlos A. Ramos et al.   CANCER JOURNAL

Adoptive Therapy With Chimeric Antigen Receptor–Modified T Cells of Defined Subset Composition

(2014) Stanley R. Riddell et al.   CANCER JOURNAL

Chimeric Antigen Receptor T Cells for Sustained Remissions in Leukemia

(2014) Shannon L. Maude et al.   NEW ENGLAND JOURNAL OF MEDICINE

Specific HIV integration sites are linked to clonal expansion and persistence of infected cells

(2014) F. Maldarelli et al.   SCIENCE

Efficacy and Toxicity Management of 19-28z CAR T Cell Therapy in B Cell Acute Lymphoblastic Leukemia

(2014) M. L. Davila et al.   Science Translational Medicine

Chromatin Landscapes of Retroviral and Transposon Integration Profiles

(2014) Johann de Jong et al.   PLoS Genetics

The Nonsignaling Extracellular Spacer Domain of Chimeric Antigen Receptors Is Decisive for In Vivo Antitumor Activity

(2014) M. Hudecek et al.   Cancer Immunology Research

Receptor Affinity and Extracellular Domain Modifications Affect Tumor Recognition by ROR1-Specific Chimeric Antigen Receptor T Cells

(2013) M. Hudecek et al.   CLINICAL CANCER RESEARCH

A new approach to gene therapy usingSleeping Beautyto genetically modify clinical-grade T cells to target CD19

(2013) Harjeet Singh et al.   IMMUNOLOGICAL REVIEWS

Comparison of Lentiviral and Sleeping Beauty Mediated αβ T Cell Receptor Gene Transfer

(2013) Anne-Christine Field et al.   PLoS One

Manufacture of Clinical-Grade CD19-Specific T Cells Stably Expressing Chimeric Antigen Receptor Using Sleeping Beauty System and Artificial Antigen Presenting Cells

(2013) Harjeet Singh et al.   PLoS One

Efficient Sleeping Beauty DNA Transposition From DNA Minicircles

(2013) Nynne Sharma et al.   Molecular Therapy-Nucleic Acids

Minicircle DNA electrotransfer for efficient tissue-targeted gene delivery

(2012) S Chabot et al.   GENE THERAPY

Performance of High Quality Minicircle DNA for In Vitro and In Vivo Gene Transfer

(2012) Dennis Kobelt et al.   MOLECULAR BIOTECHNOLOGY

Retargeting Sleeping Beauty Transposon Insertions by Engineered Zinc Finger DNA-binding Domains

(2012) Katrin Voigt et al.   MOLECULAR THERAPY

A transgene-encoded cell surface polypeptide for selection, in vivo tracking, and ablation of engineered cells

(2011) X. Wang et al.   BLOOD

TheSleeping Beautytransposon system for clinical applications

(2011) Marta Swierczek et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

The hyperactive Sleeping Beauty transposase SB100X improves the genetic modification of T cells to express a chimeric antigen receptor

(2011) Z Jin et al.   GENE THERAPY

Translating Sleeping Beauty transposition into cellular therapies: Victories and challenges

(2010) Zsuzsanna Izsvák et al.   BIOESSAYS

The B-cell tumor-associated antigen ROR1 can be targeted with T cells modified to express a ROR1-specific chimeric antigen receptor

(2010) M. Hudecek et al.   BLOOD

Comparative Analysis of Transposable Element Vector Systems in Human Cells

(2010) Ivana Grabundzija et al.   MOLECULAR THERAPY

Gene Transfer Efficiency and Genome-Wide Integration Profiling of Sleeping Beauty, Tol2, and PiggyBac Transposons in Human Primary T Cells

(2010) Xin Huang et al.   MOLECULAR THERAPY

Genomic safe harbors permit high β-globin transgene expression in thalassemia induced pluripotent stem cells

(2010) Eirini P Papapetrou et al.   NATURE BIOTECHNOLOGY

A robust system for production of minicircle DNA vectors

(2010) Mark A Kay et al.   NATURE BIOTECHNOLOGY

Efficient nonviral Sleeping Beauty transposon-based TCR gene transfer to peripheral blood lymphocytes confers antigen-specific antitumor reactivity

(2009) P D Peng et al.   GENE THERAPY

Analysis of Lentiviral Vector Integration in HIV+ Study Subjects Receiving Autologous Infusions of Gene Modified CD4+ T Cells

(2009) Gary P Wang et al.   MOLECULAR THERAPY

Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates

(2009) Lajos Mátés et al.   NATURE GENETICS

Redirecting Specificity of T-Cell Populations For CD19 Using the Sleeping Beauty System

(2008) H. Singh et al.   CANCER RESEARCH

Dynamic Regulation of Nucleosome Positioning in the Human Genome

(2008) Dustin E. Schones et al.   CELL

Minicircle-DNA production by site specific recombination and protein-DNA interaction chromatography

(2008) Peter Mayrhofer et al.   JOURNAL OF GENE MEDICINE

Sleeping Beauty Transposon‐mediated Engineering of Human Primary T Cells for Therapy of CD19+ Lymphoid Malignancies

(2008) Xin Huang et al.   MOLECULAR THERAPY