CRISPRthripsis: The Risk of CRISPR/Cas9-induced Chromothripsis in Gene Therapy

High-speed fluorescence image–enabled cell sorting

(2022) Daniel Schraivogel et al.   SCIENCE

Current Methods and Pipelines for Image-Based Quantitation of Nuclear Shape and Nuclear Envelope Abnormalities

(2022) Anne F. J. Janssen et al.   Cells

ON-Target Adverse Events of CRISPR-Cas9 Nuclease: More Chaotic than Expected

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Insight into the Molecular Basis Underlying Chromothripsis

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Cas9 exo-endonuclease eliminates chromosomal translocations during genome editing

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Correction of β-thalassemia by CRISPR/Cas9 editing of the α-globin locus in human hematopoietic stem cells

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Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance

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Chromothripsis as an on-target consequence of CRISPR–Cas9 genome editing

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CaBagE: A Cas9-based Background Elimination strategy for targeted, long-read DNA sequencing

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The in vitro micronucleus assay using imaging flow cytometry and deep learning

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Inter-laboratory automation of the in vitro micronucleus assay using imaging flow cytometry and deep learning

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Tissue Specific DNA Repair Outcomes Shape the Landscape of Genome Editing

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Whole chromosome loss and genomic instability in mouse embryos after CRISPR-Cas9 genome editing

(2021) Stamatis Papathanasiou et al.   Nature Communications

Hi-C detects genomic structural variants in peripheral blood of pediatric leukemia patients

(2021) Claire Mallard et al.   Cold Spring Harbor Molecular Case Studies

Targeted nanopore sequencing with Cas9-guided adapter ligation

(2020) Timothy Gilpatrick et al.   NATURE BIOTECHNOLOGY

Comprehensive analysis of chromothripsis in 2,658 human cancers using whole-genome sequencing

(2020) Isidro Cortés-Ciriano et al.   NATURE GENETICS

Strategies to Protect Hematopoietic Stem Cells from Culture-Induced Stress Conditions

(2020) Fatima Aerts-Kaya   Current Stem Cell Research & Therapy

Editing a γ-globin repressor binding site restores fetal hemoglobin synthesis and corrects the sickle cell disease phenotype

(2020) Leslie Weber et al.   Science Advances

An Automated, Single Cell Quantitative Imaging Microscopy Approach to Assess Micronucleus Formation, Genotoxicity and Chromosome Instability

(2020) Chloe C. Lepage et al.   Cells

Variability in Genome Editing Outcomes: Challenges for Research Reproducibility and Clinical Safety

(2020) Lydia Teboul et al.   MOLECULAR THERAPY

Mechanisms generating cancer genome complexity from a single cell division error

(2020) Neil T. Umbreit et al.   SCIENCE

Long-term fate of etoposide-induced micronuclei and micronucleated cells in Hela-H2B-GFP cells

(2020) Hauke Reimann et al.   ARCHIVES OF TOXICOLOGY

Ex vivo editing of human hematopoietic stem cells for erythroid expression of therapeutic proteins

(2020) Giulia Pavani et al.   Nature Communications

A cell cycle-dependent CRISPR-Cas9 activation system based on an anti-CRISPR protein shows improved genome editing accuracy

(2020) Daisuke Matsumoto et al.   Communications Biology

Allele-Specific Chromosome Removal after Cas9 Cleavage in Human Embryos

(2020) Michael V. Zuccaro et al.   CELL

Chromothripsis drives the evolution of gene amplification in cancer

(2020) Ofer Shoshani et al.   NATURE

CRISPR-Cas9 Gene Editing for Sickle Cell Disease and β-Thalassemia

(2020) Haydar Frangoul et al.   NEW ENGLAND JOURNAL OF MEDICINE

Precise Gene Editing Preserves Hematopoietic Stem Cell Function following Transient p53-Mediated DNA Damage Response

(2019) Giulia Schiroli et al.   Cell Stem Cell

Micronuclei and Genome Chaos: Changing the System Inheritance

(2019) Christine J. Ye et al.   Genes

Induction of fetal hemoglobin synthesis by CRISPR/Cas9-mediated editing of the human β-globin locus

(2018) Chiara Antoniani et al.   BLOOD

Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

(2018) Michael Kosicki et al.   NATURE BIOTECHNOLOGY

Altered nuclear envelope structure and proteasome function of micronuclei

(2018) Kendra K. Maass et al.   EXPERIMENTAL CELL RESEARCH

Optimization of CRISPR/Cas9 Delivery to Human Hematopoietic Stem and Progenitor Cells for Therapeutic Genomic Rearrangements

(2018) Annalisa Lattanzi et al.   MOLECULAR THERAPY

Improving Gene Editing Outcomes in Human Hematopoietic Stem and Progenitor Cells by Temporal Control of DNA Repair

(2018) Anastasia Lomova et al.   STEM CELLS

CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome

(2017) Ha Youn Shin et al.   Nature Communications

p53 Prohibits Propagation of Chromosome Segregation Errors that Produce Structural Aneuploidies

(2017) Mar Soto et al.   Cell Reports

Entosis Is Induced by Glucose Starvation

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Efficient Ex Vivo Engineering and Expansion of Highly Purified Human Hematopoietic Stem and Progenitor Cell Populations for Gene Therapy

(2017) Erika Zonari et al.   Stem Cell Reports

Defining and improving the genome-wide specificities of CRISPR–Cas9 nucleases

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Mapping the 3D genome: Aiming for consilience

(2016) Job Dekker   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Post-translational Regulation of Cas9 during G1 Enhances Homology-Directed Repair

(2016) Tony Gutschner et al.   Cell Reports

Enhancing Hematopoietic Stem Cell Transplantation Efficacy by Mitigating Oxygen Shock

(2015) Charlie R. Mantel et al.   CELL

Detailed Phenotypic and Molecular Analyses of Genetically Modified Mice Generated by CRISPR-Cas9-Mediated Editing

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Genome chaos: Survival strategy during crisis

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Punctuated Evolution of Prostate Cancer Genomes

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Chromothripsis in congenital disorders and cancer: similarities and differences

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Karyotype Heterogeneity and Unclassified Chromosomal Abnormalities

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Single-cell Hi-C reveals cell-to-cell variability in chromosome structure

(2013) Takashi Nagano et al.   NATURE

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Massive Genomic Rearrangement Acquired in a Single Catastrophic Event during Cancer Development

(2011) Philip J. Stephens et al.   CELL

Chromosome Catastrophes Involve Replication Mechanisms Generating Complex Genomic Rearrangements

(2011) Pengfei Liu et al.   CELL

Diverse system stresses: common mechanisms of chromosome fragmentation

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A Distinctive DNA Damage Response in Human Hematopoietic Stem Cells Reveals an Apoptosis-Independent Role for p53 in Self-Renewal

(2010) Michael Milyavsky et al.   Cell Stem Cell

Gene amplification as double minutes or homogeneously staining regions in solid tumors: Origin and structure

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An in vitro micronucleus assay with size-classified micronucleus counting to discriminate aneugens from clastogens

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Reversibility of apoptosis in cancer cells

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