Review
Chemistry, Analytical
Lulu Xu, Jiaxin Duan, Junman Chen, Shijia Ding, Wei Cheng
Summary: RCA is an efficient enzymatic isothermal reaction using circular probes to amplify circular templates rapidly, with high specificity and the ability to generate customized functional nucleic acids for in situ detection.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Chao Li, Mengyang Zhou, Haoyu Wang, Jie Wang, Lin Huang
Summary: This study developed a colorimetric biosensor for the visual detection of exosomes, with dual signal amplification using rolling circle amplification (RCA). The method exhibited high sensitivity and specificity, and showed potential for clinical diagnosis in distinguishing healthy individuals from leukemia patients.
Article
Chemistry, Analytical
Yuhan Wang, Jiaxuan Xiao, Xiaona Lin, Amira Waheed, Ayyanu Ravikumar, Zhen Zhang, Yanmin Zou, Chengshui Chen
Summary: A sensitive biosensor based on rolling circle amplification (RCA) was developed for colorimetric quantification of lead ion (Pb2+). The biosensor utilized GR5 DNAzymes modified on immunomagnetic beads to capture Pb2+ and initiate an RCA for improved sensitivity. The biosensor also generated a G4-hemin DNAzyme as a colorimetric signal, which showed potential in rapid detection of heavy metals in the environment.
Article
Chemistry, Analytical
Zezhou Yang, Yu Guo, Jie Zhou, Fang Liu, Wenbin Liang, Yaqin Chai, Zhaohui Li, Ruo Yuan
Summary: In this study, an innovative fluorescent sensor was developed for precise and ultrasensitive detection and imaging of target miRNA-21. The sensor utilized a dextrous target-motivated polymerization/nicking DNA nanomachineries and a hyperbranched rolling circle amplification (HB-RCA)-assisted multiposition strand displacement reaction (SDR) signal amplification approach. The technique achieved target recycling through polymerization/nicking DNA nanomachineries and HB-RCA amplification induced by the released transformation target. HB-RCA was exploited to increase the local concentration and collision efficiency of the templates and primers, generating multiple repeated DNA sequences as initiators for SDR, which improved the signal amplification multiplier. The technique effectively distinguished target miRNA-21 and detected drug-manipulative miRNA expression level abnormities. The proposed cascade nucleic acid amplification strategy could offer a promising pathway for ultrasensitive imaging of diverse biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Chenxin Fang, Ping Ouyang, Yuxing Yang, Yang Qing, Jialun Han, Wenyan Shang, Yubing Chen, Jie Du
Summary: A microRNA detection platform utilizing a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system has been proposed for rapid and efficient detection of miRNA-21. This system achieves signal amplification and fluorescence intensity amplification through different mechanisms, showing broad prospects in nucleic acid analysis and detection.
Article
Biochemical Research Methods
Guang Li, Shengjie Ge, Ping Niu, Jianyou Zhang, Yu Mao, Youwei Wang, Aidong Sun
Summary: A high-throughput surface-enhanced Raman scattering-based lateral flow assay is proposed for accurate quantification of ctDNA associated with head and neck squamous cell carcinoma. The method combines high-performing SERS probes and target-specific signal amplification strategy, enabling fast and parallel detection of multiple samples.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Chemistry, Analytical
Juan Song, Shan Li, Jie Zhou, Qiao Yu, Xue-Jiao Yang, Hong-Yuan Chen, Jing-Juan Xu
Summary: We have developed a triple signal amplification strategy for in situ visualization of telomerase RNA (TR) in living cells. The strategy includes target-initiated strand displacement circuit, multidirectional rolling circle amplification (RCA), and Mg2+ DNAzyme-mediated amplification. The proposed method exhibited ultrasensitive detection of TR mimic with a detection limit at the picomolar level. Moreover, after transfection with the proposed sensing platform, tumor cells can be easily distinguished from normal cells based on TR abundance-related fluorescence signal, providing new insights into initial cancer screening.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Zizhong Yang, Birong Liu, Ting Huang, Mengxu Sun, Tong Li, Wen-Jun Duan, Min-Min Li, Jin-Xiang Chen, Zong Dai, Jun Chen
Summary: This study developed a novel DNA nanowire technology for highly sensitive and highly specific imaging of miRNA in living cells. The technology demonstrated high reaction kinetics and sensitivity, and could detect miRNA as low as 51 pM. Additionally, it could accurately distinguish the miRNA expression difference between normal cells and cancer cells, and simultaneously image the total amount of multiple miRNAs.
Article
Biophysics
Sidan Wang, Haojia Li, Kejun Dong, Wan Shu, Jiarui Zhang, Jun Zhang, Rong Zhao, Sitian Wei, Dilu Feng, Xianjin Xiao, Wei Zhang
Summary: Point of care testing (POCT) plays a crucial role in disease diagnosis and prognosis evaluation. The biosensor based on CRISPR/Cas12a is a powerful diagnostic tool due to its high sensitivity. However, its limited recognition ability and high cost hinder its universal application. Therefore, it is important to develop a CRISPR/Cas12a-based RNA detection system that is easy to use, cheap, specific, and universal.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Linying Yu, Liping Zhu, Yao Peng, Mengting Sheng, Jianshe Huang, Xiurong Yang
Summary: A highly sensitive and versatile dual-amplification electrochemiluminescence (ECL) biosensing platform was developed for rapid and sensitive detection of biomarkers. The platform utilized DNA nanostructures and catalyzed hairpin assembly (CHA) to achieve high amplification efficiency. The biosensor demonstrated a detection limit of 1.09 fM for target DNA and showed selective detection of silver ions. The proposed strategy has potential applications in disease diagnosis and environmental monitoring.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Ju Liu, Guoyang Xie, Sidi Lv, Qin Xiong, Hengyi Xu
Summary: Isothermal nucleic acid amplification techniques are crucial diagnostic tools for clinical applications and food safety inspections. Rolling circle amplification (RCA) is a highly efficient and robust method that meets the convenient amplification requirements for point-of-care testing. This review introduces the principles, species, and optimization strategies of RCA, and summarizes recent achievements in the design, development, and application of RCA biosensors. Additionally, the versatile application of RCA in DNA nanotechnology for bioimaging, drug delivery, and therapeutics is discussed. The current challenges and future perspectives of the RCA technique are also addressed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Bingzhi Li, Anqi Xia, Siying Xie, Lei Lin, Zhirun Ji, Tiying Suo, Xing Zhang, He Huang
Summary: A signal-amplified detection method of FEN1 based on cleavage-induced ligation and rolling circle amplification was developed in this study, achieving highly sensitive and specific detection of FEN1 in real samples, as well as evaluating the inhibitory effects of chemicals on FEN1. This novel biosensor method enriches the tools for FEN1-based diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Georgia Diakoudi, Paolo Capozza, Gianvito Lanave, Francesco Pellegrini, Barbara Di Martino, Gabriella Elia, Nicola Decaro, Michele Camero, Paola Ghergo, Fabio Stasi, Alessandra Cavalli, Maria Tempesta, Vanessa R. Barrs, Julia Beatty, Krisztian Banyai, Cristiana Catella, Maria S. Lucente, Alessio Buonavoglia, Giovanna Fusco, Vito Martella
Summary: Hepadnaviruses can be found in dogs, similar to the hepadnavirus in domestic cats. Canine hepadnavirus can cause altered hepatic markers, but its role in canine health remains unclear.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Xiaoyue Zhang, Xueying Zhu, Yuanfang Li, Xin Hai, Sai Bi
Summary: In this study, a colorimetric/photothermal dual-mode exosomes sensing platform was developed for the detection of human breast cancer cell (MCF-7)-derived exosomes. The platform utilizes the encapsulation of 3,3',5,5'-tetramethylbenzidine-loaded graphene quantum dot nanozymes (TMB-GQDzymes) into DNA flowers (DFs) via rolling circle amplification (RCA). The dual-aptamer recognition strategy enables specific detection, and the platform showed excellent performance in distinguishing breast cancer patients from healthy individuals in serum samples analysis.
Article
Chemistry, Analytical
Luqi Huang, Ning Yuan, Wei Guo, Yunzhe Zhang, Wei Zhang
Summary: A novel electrochemical biosensor based on saltatory rolling circle amplification (SRCA) and CRISPR/Cas12a system was developed for accurate detection of Staphylococcus aureus. The biosensor showed high sensitivity, specificity, and accuracy, providing a potential powerful platform for the detection of S. aureus.
Article
Chemistry, Analytical
Xiaonan Wang, Ying Liu, Juan Zhang, Genxi Li
SENSORS AND ACTUATORS B-CHEMICAL
(2017)
Article
Electrochemistry
Jing Zhao, Yingying Tang, Ya Cao, Tianshu Chen, Xiaoxia Chen, Xiaoxia Mao, Yongmei Yin, Guifang Chen
ELECTROCHIMICA ACTA
(2018)
Article
Chemistry, Analytical
Xiaoxia Mao, Yifei Li, Peng Han, Xiaohong Wang, Shiqi Yang, Fan Zhang, Xiuqing Gong, Ya Cao
SENSORS AND ACTUATORS B-CHEMICAL
(2018)
Article
Chemistry, Multidisciplinary
Chao Li, Xiaolu Hu, Jianyang Lu, Xiaoxia Mao, Yang Xiang, Yongqian Shu, Genxi Li
Article
Chemistry, Multidisciplinary
Xiaoxia Mao, Guifang Chen, Zihan Wang, Yuanguang Zhang, Xiaoli Zhu, Genxi Li
Article
Medicine, Research & Experimental
Xiaoxia Chen, Jing Zhao, Tianshu Chen, Tao Gao, Xiaoli Zhu, Genxi Li
Article
Chemistry, Analytical
Xiaoxia Mao, Shaobing Pan, Duoqi Zhou, Xiaowu He, Yuanguang Zhang
SENSORS AND ACTUATORS B-CHEMICAL
(2019)
Article
Nanoscience & Nanotechnology
Xiaoxia Mao, Dongsheng Mao, Tianshu Chen, Mohammed Jalalah, Mohammed S. Al-Assiri, Farid A. Harraz, Xiaoli Zhu, Genxi Li
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Chemistry, Analytical
Xiaoxia Mao, Shaowei Liu, Benyue Su, Dejin Wang, Zhan Huang, Jian Li, Yuanguang Zhang
Article
Chemistry, Multidisciplinary
Shaowei Liu, Yumeng Yang, Mengqin Shi, Hai Shi, Dongsheng Mao, Xiaoxia Mao, Yuanguang Zhang
Summary: In this study, a portable smartphone-based DNAzyme hydrogel platform was developed for the detection of cfDNA. The platform showed high sensitivity and visibility, making it a promising tool for testing other nucleic acid biomarkers in the future.
Article
Chemistry, Analytical
Yuxin Lin, Beibei Nie, Xinyu Qu, Minghui Wang, Jie Yang, Genxi Li
Summary: Covalent organic frameworks (COFs) have attracted much attention for their unique physical-chemical characteristics and potential applications in biosensing. In this study, spherical COFs with regular shape and good dispersion were synthesized, and a novel nanoprobe was developed by modifying Histostar on the COFs surface. The nanoprobe was then applied to fabricate an electrochemical biosensor for exosomes detection, achieving a low detection limit and wide linear detection range. This work offers a promising platform for ultrasensitive detection of exosomes.
Article
Biochemical Research Methods
Xiaoxia Mao, Dongsheng Mao, Juanjuan Jiang, Benyue Su, Guifang Chen, Xiaoli Zhu
Summary: In this study, a semi-dry smart biosensing platform was developed for the sensitive detection of metal ions using a hydrogel and intelligent image recognition. The platform demonstrated favorable operability and performance, with detection limits of 0.34 mM for potassium ions and 5.6 nM for mercury ions. It was also capable of detecting ions in serum and lake water, showing promising potential for on-site testing.
Article
Nanoscience & Nanotechnology
Tao Gao, Chaoli Mu, Hai Shi, Liu Shi, Xiaoxia Mao, Genxi Li
ACS APPLIED MATERIALS & INTERFACES
(2018)
Article
Chemistry, Analytical
Xiaoli Zhu, Yaoyao Chen, Chang Feng, Wei Wang, Bing Bo, Ruixin Ren, Genxi Li
ANALYTICAL CHEMISTRY
(2017)
Article
Biophysics
Jinlong Li, Tao Gao, Shiyu Gu, Jun Zhi, Jie Yang, Genxi Li
BIOSENSORS & BIOELECTRONICS
(2017)