Recent applications of rolling circle amplification in biosensors and DNA nanotechnology

Isothermal nucleic acid amplification techniques have emerged as an important diagnostic tool for clinical applications and food safety inspection. Rolling circle amplification (RCA) is well known for its simple, highly efficient and robust nucleic acid amplification. Besides, RCA excels through its convenient and accessible amplification requirements, making it a promising candidate for point of care testing (POCT). In this review, we firstly introduce the basic principles of RCA cyclization methods, RCA species and RCA optimization strategies. Based on these principles and strategies, we summarize the recent achievements on the design, development and application of RCA biosensors for biosensing. Moreover, we discuss the versatile application of RCA in DNA nanotechnology, where RCA acts as a programable DNA nanomaterials construction tool for bioimaging, drug delivery and therapeutics. Finally, we discuss the current challenges and future perspectives of the RCA technique.(c) 2023 Elsevier B.V. All rights reserved.

Automated summary

Isothermal nucleic acid amplification techniques are crucial diagnostic tools for clinical applications and food safety inspections. Rolling circle amplification (RCA) is a highly efficient and robust method that meets the convenient amplification requirements for point-of-care testing. This review introduces the principles, species, and optimization strategies of RCA, and summarizes recent achievements in the design, development, and application of RCA biosensors. Additionally, the versatile application of RCA in DNA nanotechnology for bioimaging, drug delivery, and therapeutics is discussed. The current challenges and future perspectives of the RCA technique are also addressed.