Phase separation in viral infections

14-3-3 Proteins are Potential Regulators of Liquid–Liquid Phase Separation

(2022) Xianlong Huang et al.   CELL BIOCHEMISTRY AND BIOPHYSICS

O-GlcNAcylation modulates liquid–liquid phase separation of SynGAP/PSD-95

(2022) Pinou Lv et al.   Nature Chemistry

Temporal control of the integrated stress response by a stochastic molecular switch

(2022) Philipp Klein et al.   Science Advances

1,6-Hexanediol, commonly used to dissolve liquid-liquid phase separated condensates, directly impairs kinase and phosphatase activities

(2021) Robert Düster et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

(2021) Shan Lu et al.   Nature Communications

ATP biphasically modulates LLPS of SARS-CoV-2 nucleocapsid protein and specifically binds its RNA-binding domain

(2021) Mei Dang et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Liquid Biomolecular Condensates and Viral Lifecycles: Review and Perspectives

(2021) Temitope Etibor et al.   Viruses-Basel

The Mechanism of SARS-CoV-2 Nucleocapsid Protein Recognition by the Human 14-3-3 Proteins

(2021) Kristina V. Tugaeva et al.   JOURNAL OF MOLECULAR BIOLOGY

Viral tegument proteins restrict cGAS-DNA phase separation to mediate immune evasion

(2021) Guangjun Xu et al.   MOLECULAR CELL

GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein

(2021) Ming Zhao et al.   Nature Communications

Liquid–liquid phase separation underpins the formation of replication factories in rotaviruses

(2021) Florian Geiger et al.   EMBO JOURNAL

Role of Heat Shock Proteins (HSP70 and HSP90) in Viral Infection

(2021) Anna Lubkowska et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

A condensate-hardening drug blocks RSV replication in vivo

(2021) Jennifer Risso-Ballester et al.   NATURE

Targeting liquid–liquid phase separation of SARS-CoV-2 nucleocapsid protein promotes innate antiviral immunity by elevating MAVS activity

(2021) Shuai Wang et al.   NATURE CELL BIOLOGY

Targeted modulation of protein liquid–liquid phase separation by evolution of amino-acid sequence

(2021) Simon M. Lichtinger et al.   PLoS Computational Biology

New Perspectives on the Biogenesis of Viral Inclusion Bodies in Negative-Sense RNA Virus Infections

(2021) Olga Dolnik et al.   Cells

Nucleocapsid mutations R203K/G204R increase the infectivity, fitness, and virulence of SARS-CoV-2

(2021) Haibo Wu et al.   Cell Host & Microbe

Liquid-liquid phase separation: Orchestrating cell signaling through time and space

(2021) Qi Su et al.   MOLECULAR CELL

Deconstructing virus condensation

(2021) Nora Lopez et al.   PLoS Pathogens

SARS-CoV-2 nucleocapsid protein binds host mRNAs and attenuates stress granules to impair host stress response

(2021) Syed Nabeel-Shah et al.   iScience

Metabolic host response and therapeutic approaches to influenza infection

(2020) Mohsen Keshavarz et al.   CELLULAR & MOLECULAR BIOLOGY LETTERS

A SARS-CoV-2 protein interaction map reveals targets for drug repurposing

(2020) David E. Gordon et al.   NATURE

The Global Phosphorylation Landscape of SARS-CoV-2 Infection

(2020) Mehdi Bouhaddou et al.   CELL

Biomolecular Condensates in the Nucleus

(2020) Benjamin R. Sabari et al.   TRENDS IN BIOCHEMICAL SCIENCES

HIV-1 replication complexes accumulate in nuclear speckles and integrate into speckle-associated genomic domains

(2020) Ashwanth C. Francis et al.   Nature Communications

Relevance of Electrostatic Charges in Compactness, Aggregation, and Phase Separation of Intrinsically Disordered Proteins

(2020) Greta Bianchi et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Respiratory Syncytial Virus Sequesters NF-κB Subunit p65 to Cytoplasmic Inclusion Bodies To Inhibit Innate Immune Signaling

(2020) Fatoumatta Jobe et al.   JOURNAL OF VIROLOGY

RNA-binding and prion domains: the Yin and Yang of phase separation

(2020) Nieves Lorenzo Gotor et al.   NUCLEIC ACIDS RESEARCH

Molecular mechanisms of stress granule assembly and disassembly

(2020) Sarah Hofmann et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Negri bodies and other virus membrane-less replication compartments

(2020) Quentin Nevers et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

SARS‐CoV‐2 nucleocapsid protein phase‐separates with RNA and with human hnRNPs

(2020) Theodora Myrto Perdikari et al.   EMBO JOURNAL

Clustering and reverse transcription of HIV‐1 genomes in nuclear niches of macrophages

(2020) Elena Rensen et al.   EMBO JOURNAL

Liquid–Liquid Phase Separation by Intrinsically Disordered Protein Regions of Viruses: Roles in Viral Life Cycle and Control of Virus–Host Interactions

(2020) Stefania Brocca et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Genomic RNA Elements Drive Phase Separation of the SARS-CoV-2 Nucleocapsid

(2020) Christiane Iserman et al.   MOLECULAR CELL

RNA-binding proteins in human genetic disease

(2020) Fátima Gebauer et al.   NATURE REVIEWS GENETICS

Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates

(2020) Adriana Savastano et al.   Nature Communications

Influenza virus uses transportin 1 for vRNP debundling during cell entry

(2019) Yasuyuki Miyake et al.   Nature Microbiology

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

A lipid-based partitioning mechanism for selective incorporation of proteins into membranes of HIV particles

(2019) Prabuddha Sengupta et al.   NATURE CELL BIOLOGY

Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties

(2019) Steven Boeynaems et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nipah virus induces two inclusion body populations: Identification of novel inclusions at the plasma membrane

(2019) Marc Ringel et al.   PLoS Pathogens

m6A enhances the phase separation potential of mRNA

(2019) Ryan J. Ries et al.   NATURE

Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites

(2019) Marta Alenquer et al.   Nature Communications

Measles Virus Forms Inclusion Bodies with Properties of Liquid Organelles

(2019) Yuqin Zhou et al.   JOURNAL OF VIROLOGY

Phase Separation of FUS Is Suppressed by Its Nuclear Import Receptor and Arginine Methylation

(2018) Mario Hofweber et al.   CELL

Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains

(2018) Lin Guo et al.   CELL

Tau protein liquid–liquid phase separation can initiate tau aggregation

(2018) Susanne Wegmann et al.   EMBO JOURNAL

Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, Interactions, and Phase Separation Altered by Mutation and Arginine Methylation

(2018) Veronica H. Ryan et al.   MOLECULAR CELL

RNA buffers the phase separation behavior of prion-like RNA binding proteins

(2018) Shovamayee Maharana et al.   SCIENCE

DNA-induced liquid phase condensation of cGAS activates innate immune signaling

(2018) Mingjian Du et al.   SCIENCE

Coactivator condensation at super-enhancers links phase separation and gene control

(2018) Benjamin R. Sabari et al.   SCIENCE

Phase separation of a yeast prion protein promotes cellular fitness

(2018) Titus M. Franzmann et al.   SCIENCE

Importance of the 1+7 configuration of ribonucleoprotein complexes for influenza A virus genome packaging

(2018) Takeshi Noda et al.   Nature Communications

Intrinsically Disordered Regions Can Contribute Promiscuous Interactions to RNP Granule Assembly

(2018) David S.W. Protter et al.   Cell Reports

The molecular language of membraneless organelles

(2018) Edward Gomes et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Acetylation of intrinsically disordered regions regulates phase separation

(2018) Makoto Saito et al.   Nature Chemical Biology

The Stress Granule Transcriptome Reveals Principles of mRNA Accumulation in Stress Granules

(2017) Anthony Khong et al.   MOLECULAR CELL

HIV-1 NC-induced stress granule assembly and translation arrest are inhibited by the dsRNA binding protein Staufen1

(2017) Shringar Rao et al.   RNA

ATP as a biological hydrotrope

(2017) Avinash Patel et al.   SCIENCE

The innate immune response to RSV: Advances in our understanding of critical viral and host factors

(2017) Yan Sun et al.   VACCINE

Negri bodies are viral factories with properties of liquid organelles

(2017) Jovan Nikolic et al.   Nature Communications

Functional organization of cytoplasmic inclusion bodies in cells infected by respiratory syncytial virus

(2017) Vincent Rincheval et al.   Nature Communications

Synchronized HIV assembly by tunable PIP2 changes reveals PIP2 requirement for stable Gag anchoring

(2017) Frauke Mücksch et al.   eLife

Compositional Control of Phase-Separated Cellular Bodies

(2016) Salman F. Banani et al.   CELL

Phasing in and out

(2016) James Shorter   Nature Chemistry

Membraneless organelles can melt nucleic acid duplexes and act as biomolecular filters

(2016) Timothy J. Nott et al.   Nature Chemistry

HIV-1 Gag Blocks Selenite-Induced Stress Granule Assembly by Altering the mRNA Cap-Binding Complex

(2016) Alessandro Cinti et al.   mBio

Defining Hsp70 Subnetworks in Dengue Virus Replication Reveals Key Vulnerability in Flavivirus Infection

(2015) Shuhei Taguwa et al.   CELL

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

Intrinsically disordered proteins in cellular signalling and regulation

(2015) Peter E. Wright et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

An acetylation switch controls TDP-43 function and aggregation propensity

(2015) Todd J. Cohen et al.   Nature Communications

Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

Structural Disorder in Viral Proteins

(2014) Bin Xue et al.   CHEMICAL REVIEWS

Influenza A virus uses the aggresome processing machinery for host cell entry

(2014) Indranil Banerjee et al.   SCIENCE

Alternative splicing of intrinsically disordered regions and rewiring of protein interactions

(2013) Marija Buljan et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Cyclic GMP-AMP Containing Mixed Phosphodiester Linkages Is An Endogenous High-Affinity Ligand for STING

(2013) Xu Zhang et al.   MOLECULAR CELL

cGAS produces a 2′-5′-linked cyclic dinucleotide second messenger that activates STING

(2013) Andrea Ablasser et al.   NATURE

p38 and OGT Sequestration into Viral Inclusion Bodies in Cells Infected with Human Respiratory Syncytial Virus Suppresses MK2 Activities and Stress Granule Assembly

(2012) Jens Fricke et al.   JOURNAL OF VIROLOGY

Hsp70 Protein Positively Regulates Rabies Virus Infection

(2012) X. Lahaye et al.   JOURNAL OF VIROLOGY

Human Respiratory Syncytial Virus Nucleoprotein and Inclusion Bodies Antagonize the Innate Immune Response Mediated by MDA5 and MAVS

(2012) A. W. Lifland et al.   JOURNAL OF VIROLOGY

The Molecular Interactions That Stabilize RNA Tertiary Structure: RNA Motifs, Patterns, and Networks

(2011) Samuel E. Butcher et al.   ACCOUNTS OF CHEMICAL RESEARCH

A Rab11- and Microtubule-Dependent Mechanism for Cytoplasmic Transport of Influenza A Virus Viral RNA

(2011) M. J. Amorim et al.   JOURNAL OF VIROLOGY

Soft active aggregates: mechanics, dynamics and self-assembly of liquid-like intracellular protein bodies

(2011) Clifford P. Brangwynne   Soft Matter

Assembly and disassembly of the nucleolus during the cell cycle

(2011) Danièle Hernandez-Verdun   Nucleus

Respiratory Syncytial Virus Induces Host RNA Stress Granules To Facilitate Viral Replication

(2010) M. E. Lindquist et al.   JOURNAL OF VIROLOGY

The Rab11 Pathway Is Required for Influenza A Virus Budding and Filament Formation

(2010) E. A. Bruce et al.   JOURNAL OF VIROLOGY

Formation of Antiviral Cytoplasmic Granules during Orthopoxvirus Infection

(2010) M. Simpson-Holley et al.   JOURNAL OF VIROLOGY

Biophysical characterization of intrinsically disordered proteins

(2009) David Eliezer   CURRENT OPINION IN STRUCTURAL BIOLOGY

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

LEDGF/p75 Proteins with Alternative Chromatin Tethers Are Functional HIV-1 Cofactors

(2009) Anne M. Meehan et al.   PLoS Pathogens

A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly

(2008) Takbum Ohn et al.   NATURE CELL BIOLOGY

Do viral proteins possess unique biophysical features?

(2008) Nobuhiko Tokuriki et al.   TRENDS IN BIOCHEMICAL SCIENCES