Liquid–liquid phase separation underpins the formation of replication factories in rotaviruses

Designer Condensates: A Toolkit for the Biomolecular Architect

(2021) Renee L. Hastings et al.   JOURNAL OF MOLECULAR BIOLOGY

Sequence-encoded and composition-dependent protein-RNA interactions control multiphasic condensate morphologies

(2021) Taranpreet Kaur et al.   Nature Communications

Learning the molecular grammar of protein condensates from sequence determinants and embeddings

(2021) Kadi L. Saar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A condensate-hardening drug blocks RSV replication in vivo

(2021) Jennifer Risso-Ballester et al.   NATURE

Highly accurate protein structure prediction with AlphaFold

(2021) John Jumper et al.   NATURE

Viroplasms: Assembly and Functions of Rotavirus Replication Factories

(2021) Guido Papa et al.   Viruses-Basel

flDPnn: Accurate intrinsic disorder prediction with putative propensities of disorder functions

(2021) Gang Hu et al.   Nature Communications

RNA Droplets

(2020) Kevin Rhine et al.   Annual Review of Biophysics

Physical Principles Underlying the Complex Biology of Intracellular Phase Transitions

(2020) Jeong-Mo Choi et al.   Annual Review of Biophysics

Modulation of RNA Condensation by the DEAD-Box Protein eIF4A

(2020) Devin Tauber et al.   CELL

Conserved rotavirus NSP5 and VP2 domains interact and affect viroplasm

(2020) Antonino Buttafuoco et al.   JOURNAL OF VIROLOGY

TDP-43 α-helical structure tunes liquid–liquid phase separation and function

(2020) Alexander E. Conicella et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Digital Sensing and Molecular Computation by an Enzyme-Free DNA Circuit

(2020) William E. Arter et al.   ACS Nano

Discrete virus factories form in the cytoplasm of cells co-infected with two replication competent tagged reporter birnaviruses, that subsequently coalesce over time.

(2020) Elle A. Campbell et al.   JOURNAL OF VIROLOGY

Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking

(2020) Anne Monette et al.   Cell Reports

Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly

(2020) Serafima Guseva et al.   Science Advances

α-Synuclein aggregation nucleates through liquid–liquid phase separation

(2020) Soumik Ray et al.   Nature Chemistry

RNA contributions to the form and function of biomolecular condensates

(2020) Christine Roden et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

CRISPR-Csy4-Mediated Editing of Rotavirus Double-Stranded RNA Genome

(2020) Guido Papa et al.   Cell Reports

Genomic RNA Elements Drive Phase Separation of the SARS-CoV-2 Nucleocapsid

(2020) Christiane Iserman et al.   MOLECULAR CELL

Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates

(2020) Adriana Savastano et al.   Nature Communications

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties

(2019) Steven Boeynaems et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites

(2019) Marta Alenquer et al.   Nature Communications

RNA is a critical element for the sizing and the composition of phase-separated RNA–protein condensates

(2019) Marina Garcia-Jove Navarro et al.   Nature Communications

Nanoscale organization of rotavirus replication machineries

(2019) Yasel Garcés Suárez et al.   eLife

Formation of biological condensates via phase separation: Characteristics, analytical methods, and physiological implications

(2019) Zhe Feng et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

(2019) Daniel J. Nieves et al.   Royal Society Open Science

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

Microtubules as platforms for probing liquid–liquid phase separation in cells – application to RNA-binding proteins

(2018) Alexandre Maucuer et al.   JOURNAL OF CELL SCIENCE

Mechanics, Structure and Function of Biopolymer Condensates

(2018) Louis-Philippe Bergeron-Sandoval et al.   JOURNAL OF MOLECULAR BIOLOGY

Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation

(2018) Tomohiro Yamazaki et al.   MOLECULAR CELL

Stability of local secondary structure determines selectivity of viral RNA chaperones

(2018) Jack P K Bravo et al.   NUCLEIC ACIDS RESEARCH

BeStSel: a web server for accurate protein secondary structure prediction and fold recognition from the circular dichroism spectra

(2018) András Micsonai et al.   NUCLEIC ACIDS RESEARCH

A New Lens for RNA Localization: Liquid-Liquid Phase Separation

(2018) Erin M. Langdon et al.   Annual Review of Microbiology

Emerging Roles for Intermolecular RNA-RNA Interactions in RNP Assemblies

(2018) Briana Van Treeck et al.   CELL

Who's In and Who's Out—Compositional Control of Biomolecular Condensates

(2018) Jonathon A. Ditlev et al.   JOURNAL OF MOLECULAR BIOLOGY

Phase Transitions Drive the Formation of Vesicular Stomatitis Virus Replication Compartments

(2018) Bianca S. Heinrich et al.   mBio

Genome packaging in multi-segmented dsRNA viruses: distinct mechanisms with similar outcomes

(2018) Alexander Borodavka et al.   Current Opinion in Virology

An ultraweak interaction in the intrinsically disordered replication machinery is essential for measles virus function

(2018) Sigrid Milles et al.   Science Advances

Phosphorylation cascade regulates the formation and maturation of rotaviral replication factories

(2018) Jeanette M. Criglar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Quantitative analysis of multilayer organization of proteins and RNA in nuclear speckles at super resolution

(2017) Jingyi Fei et al.   JOURNAL OF CELL SCIENCE

Identification of a Small Molecule That Compromises the Structural Integrity of Viroplasms and Rotavirus Double-Layered Particles

(2017) Catherine Eichwald et al.   JOURNAL OF VIROLOGY

The Stress Granule Transcriptome Reveals Principles of mRNA Accumulation in Stress Granules

(2017) Anthony Khong et al.   MOLECULAR CELL

Phase behaviour of disordered proteins underlying low density and high permeability of liquid organelles

(2017) Ming-Tzo Wei et al.   Nature Chemistry

Super-resolution microscopy with DNA-PAINT

(2017) Joerg Schnitzbauer et al.   Nature Protocols

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Negri bodies are viral factories with properties of liquid organelles

(2017) Jovan Nikolic et al.   Nature Communications

Protein-mediated RNA folding governs sequence-specific interactions between rotavirus genome segments

(2017) Alexander Borodavka et al.   eLife

Mechanisms and Consequences of Macromolecular Phase Separation

(2016) Louis-Philippe Bergeron-Sandoval et al.   CELL

Toxic PR Poly-Dipeptides Encoded by the C9orf72 Repeat Expansion Target LC Domain Polymers

(2016) Yi Lin et al.   CELL

Coexisting Liquid Phases Underlie Nucleolar Subcompartments

(2016) Marina Feric et al.   CELL

Membraneless organelles can melt nucleic acid duplexes and act as biomolecular filters

(2016) Timothy J. Nott et al.   Nature Chemistry

Optical imaging of individual biomolecules in densely packed clusters

(2016) Mingjie Dai et al.   Nature Nanotechnology

Lipid droplets form complexes with viroplasms and are crucial for rotavirus replication

(2016) Sue E Crawford et al.   Current Opinion in Virology

Distinct stages in stress granule assembly and disassembly

(2016) Joshua R Wheeler et al.   eLife

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

(2015) Avinash Patel et al.   CELL

Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins

(2015) Yuan Lin et al.   MOLECULAR CELL

Phosphorylation-mediated RNA/peptide complex coacervation as a model for intracellular liquid organelles

(2015) William M. Aumiller et al.   Nature Chemistry

Polymer physics of intracellular phase transitions

(2015) Clifford P. Brangwynne et al.   Nature Physics

Floating Droplet Array: An Ultrahigh-Throughput Device for Droplet Trapping, Real-time Analysisand Recovery

(2015) Louai Labanieh et al.   Micromachines

Probing the Sites of Interactions of Rotaviral Proteins Involved in Replication

(2014) M. Viskovska et al.   JOURNAL OF VIROLOGY

Folding of an intrinsically disordered protein by phosphorylation as a regulatory switch

(2014) Alaji Bah et al.   NATURE

Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT

(2014) Ralf Jungmann et al.   NATURE METHODS

The amyloid state and its association with protein misfolding diseases

(2014) Tuomas P. J. Knowles et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Virus-Mediated Compartmentalization of the Host Translational Machinery

(2014) E. A. Desmet et al.   mBio

A Novel Form of Rotavirus NSP2 and Phosphorylation-Dependent NSP2-NSP5 Interactions Are Associated with Viroplasm Assembly

(2013) J. M. Criglar et al.   JOURNAL OF VIROLOGY

Single-cell analysis and sorting using droplet-based microfluidics

(2013) Linas Mazutis et al.   Nature Protocols

Further characterisation of rotavirus cores: Ss(+)RNAs can be packaged in vitro but packaging lacks sequence specificity

(2013) Ulrich Desselberger et al.   VIRUS RESEARCH

Crystallographic Analysis of Rotavirus NSP2-RNA Complex Reveals Specific Recognition of 5' GG Sequence for RTPase Activity

(2012) L. Hu et al.   JOURNAL OF VIROLOGY

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Structural insights into the coupling of virion assembly and rotavirus replication

(2012) Shane D. Trask et al.   NATURE REVIEWS MICROBIOLOGY

Rotavirus Viroplasm Fusion and Perinuclear Localization Are Dynamic Processes Requiring Stabilized Microtubules

(2012) Catherine Eichwald et al.   PLoS One

Structural Organisation of the Rotavirus Nonstructural Protein NSP5

(2011) Davy Martin et al.   JOURNAL OF MOLECULAR BIOLOGY

Soft active aggregates: mechanics, dynamics and self-assembly of liquid-like intracellular protein bodies

(2011) Clifford P. Brangwynne   Soft Matter

Rotavirus NSP5 orchestrates recruitment of viroplasmic proteins

(2010) R. Contin et al.   JOURNAL OF GENERAL VIROLOGY

Rotaviruses Associate with Cellular Lipid Droplet Components To Replicate in Viroplasms, and Compounds Disrupting or Blocking Lipid Droplets Inhibit Viroplasm Formation and Viral Replication

(2010) W. Cheung et al.   JOURNAL OF VIROLOGY

Characterization of viroplasm formation during the early stages of rotavirus infection

(2010) José J Carreño-Torres et al.   Virology Journal

Influence of the Partitioning of Osmolytes by the Cytoplasm on the Passive Response of Cells to Osmotic Loading

(2009) Michael B. Albro et al.   BIOPHYSICAL JOURNAL

Analysis of rotavirus non-structural protein NSP5 by mass spectrometry reveals a complex phosphorylation pattern

(2009) Pablo H. Sotelo et al.   VIRUS RESEARCH

Mechanism for Coordinated RNA Packaging and Genome Replication by Rotavirus Polymerase VP1

(2008) Xiaohui Lu et al.   STRUCTURE