Article
Chemistry, Multidisciplinary
Maria Jose Garcia-Jimenez, Sergio Gil-Caballero, Susana Maza, Francisco Corzana, Francisco Juarez-Vicente, Jonathan R. Miles, Kazuma Sakamoto, Kenji Kadomatsu, Mario Garcia-Dominguez, Jose L. de Paz, Pedro M. Nieto
Summary: This study illustrates the interactions between Midkine and chondroitin sulfate, demonstrating that their basic shape is controlled by the geometry of the glycosidic linkages. The ligand orientation within the complex is shown to be highly variable, with Docking analysis confirming the participation of aromatic residues from Midkine. Additionally, the study tested the biological activity of increasing MK expression using CS tetrasaccharides, enhancing the growth stimulation effect of MK in NIH3T3 cells.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Eathen O. Ryan, Zhoumai Jiang, Hoa Nguyen, Xu Wang
Summary: This study investigated the interaction between PTN and heparin hexasaccharide using NMR and found that PTN's arginines, lysines, and tryptophans have strong interactions with the 2-O-sulfated uronate protons in heparin hexasaccharide.
Article
Plant Sciences
Shuang Yang, Guang-Yan Zhang, Jin-Yue Zhang, Tian-Qi Li, Zhe-Hui Zhao, Ying-Hong Wang, Ping-Sheng Lei
Summary: A total synthesis approach of CS-E oligosaccharides was established and a series of derivatives were synthesized. These oligosaccharides were evaluated for their interactions with cytokines, midkine, and pleiotrophin. The binding epitopes of the oligosaccharides to midkine were mapped, and it was found that the groups on the reducing end contributed to the binding affinity.
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Cristina Di Carluccio, Maria Concetta Forgione, Silvia Martini, Francesco Berti, Antonio Molinaro, Roberta Marchetti, Alba Silipo
Summary: Molecular recognition is crucial in biology, and the combination of NMR techniques with computational methods allows for the accurate determination of protein-ligand complex 3D models, providing key structural insights into ligand-receptor interactions.
CARBOHYDRATE RESEARCH
(2021)
Article
Cell Biology
Yi-Fan Huang, Shuji Mizumoto, Morihisa Fujita
Summary: The study developed a comprehensive glycosylation mapping tool, GlycoMaple, and analyzed the expression levels of GAG biosynthetic genes in various human tissues and tumor tissues using this tool.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Bin Zhang, Lianli Chi
Summary: Chondroitin sulfate and dermatan sulfate are linear anionic polysaccharides that play important roles in physiological and pathological processes through their interactions with various proteins.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Adeola E. Awofiranye, Jon Hudson, Aditi Dey Tithi, Robert J. Linhardt, Wanwipa Vongsangnak, Mattheos A. G. Koffas
Summary: This review discusses the structure, medical significance, and limitations of current extraction methods of chondroitin sulfate (CS). It explores alternative production strategies for CS, including chemical synthesis and chemoenzymatic synthesis, with a focus on the successful metabolic engineering of strains. Microbial engineering is presented as the optimal option for modern production of chondroitin and CS, and the biosynthetic pathway for chondroitin production in various microbes is also discussed. Manipulation of pathway genes has led to the biosynthesis of chondroitin derivatives.
FERMENTATION-BASEL
(2022)
Article
Chemistry, Analytical
Qiang Ren, Jian Wang, Chao Liu, Ling-xin Meng, Rui-kun Qian, Hui-jie Gao, Wei Qin, Cai-ju Zhou, Sen Qiao, Hui-yun Wang, Li-tao Zhang, Yun-tao Zhang
Summary: This study found that pancreatic tumor tissues exhibit increased sulfation levels, particularly Delta Di-4S and Delta Di-6S, while the changes in KS were not significant. The expression levels of CHST1 and CHST4 were significantly increased and could be used to differentiate between healthy and cancerous tissues. The up-regulation of CHST3, CHST12, CHST13, and CHST15 was directly correlated with sulfation.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Huimin Pang, Jinwen Li, Zhiwei Miao, Shu Jie Li
Summary: The study found that chondroitin sulfates from shark and pig exhibit significant inhibitory effects on alpha-amylase activity and effectively reduce blood glucose levels, making them suitable as novel functional food ingredients for managing Type-II diabetes mellitus.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemical Research Methods
Salome Poyer, Ilham Seffouh, Chrystel Lopin-Bon, Jean-Claude Jacquinet, Jose L. Neira, Jean-Yves Salpin, Regis Daniel
Summary: The study developed a new analytical method for efficient differentiation and sensitive detection of different structures of chondroitin sulfate glycosaminoglycans, which was applied to analyze the disaccharide composition of biological reaction media.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Hisatoshi Hanamatsu, Satoshi Makino, Masatsugu Ohara, Goki Suda, Ikuko Yokota, Shoko Nishihara, Naoya Sakamoto, Jun-ichi Furukawa
Summary: In this study, the analysis of 2-aminobenzamide (2-AB) labeled GAG disaccharides using HPLC with a RP-column with adamantyl groups was examined. The method allowed for the separation and quantification of 17 types of GAG disaccharides from various biological samples, with fast analysis time and high retention time reproducibility.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Food Science & Technology
Ruchira Ghosh, Donald S. S. Thomas, Jayashree Arcot
Summary: This study investigates the molecular recognition patterns between cyanocobalamin and different plant and animal proteins using STD-NMR and molecular docking. Results show that myoglobin has the highest binding affinity while gluten has the lowest. Casein also exhibits higher binding affinity compared to plant-based proteins. Computer simulation validates the experimental results. This work is important for understanding the binding affinity and biological action of cyanocobalamin and encourages the use of NMR technology in studying the chemical and physiological properties of nutrients.
Article
Chemistry, Applied
Laura Di Muzio, Patrizia Paolicelli, Jordan Trilli, Stefania Petralito, Vito Cosimo Carriero, Chiara Brandelli, Mattia Spano, Anatoly Petrovich Sobolev, Luisa Mannina, Maria Antonietta Casadei
Summary: In this study, the methacrylation reaction of chondroitin sulfate (CS) was optimized to achieve tunable and reproducible degrees of methacrylation. NMR spectroscopy analysis revealed that the reaction proceeds via both epoxy ring-opening and transesterification, preserving the functional groups of CS.
CARBOHYDRATE POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
Annamaria Sandomenico, Lorenzo Di Rienzo, Luisa Calvanese, Emanuela Iaccarino, Gabriella D'Auria, Lucia Falcigno, Angela Chambery, Rosita Russo, Guido Franzoso, Laura Tornatore, Marco D'Abramo, Menotti Ruvo, Edoardo Milanetti, Domenico Raimondo
Summary: The GADD45 beta/MKK7 complex plays a crucial role in multiple myeloma, and the inhibitor DTP3 can effectively kill cancer cells without harming normal cells. Research has identified the N-terminal region of MKK7 as the optimal binding site explored by DTP3.
Article
Chemistry, Multidisciplinary
Thorben Maass, George Ssebyatika, Marlene Brueckner, Lea Breckwoldt, Thomas Krey, Alvaro Mallagaray, Thomas Peters, Martin Frank, Robert Creutznacher
Summary: This study used NMR experiments to investigate the binding of selected glycans and glycomimetics to the SARS CoV-2 spike glycoprotein and its receptor binding domain. The results revealed strong binding between sialoglycans and the Wuhan strain S-protein, weak binding between sialoglycans and the Omicron/BA.1 S-protein, and no binding between sialoglycans and the Delta 143-145 deletion mutant of the Wuhan S-protein. Additionally, the glycomimetics Oseltamivir and Zanamivir exhibited weak binding to the S-protein of both virus strains.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Maria Jose Garcia-Jimenez, Sergio Gil-Caballero, Susana Maza, Francisco Corzana, Francisco Juarez-Vicente, Jonathan R. Miles, Kazuma Sakamoto, Kenji Kadomatsu, Mario Garcia-Dominguez, Jose L. de Paz, Pedro M. Nieto
Summary: This study illustrates the interactions between Midkine and chondroitin sulfate, demonstrating that their basic shape is controlled by the geometry of the glycosidic linkages. The ligand orientation within the complex is shown to be highly variable, with Docking analysis confirming the participation of aromatic residues from Midkine. Additionally, the study tested the biological activity of increasing MK expression using CS tetrasaccharides, enhancing the growth stimulation effect of MK in NIH3T3 cells.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Javier Rojo, Pedro M. Nieto, Jose L. de Paz
Summary: Langerin is a C-type Lectin expressed on Langerhans cells that plays a crucial role in immune defense. It has the ability to recognize various carbohydrate ligands, including sulfated glycosaminoglycans (GAGs). Although there have been advancements in the synthesis of carbohydrate multivalent systems mimicking GAGs, there is limited research on their interaction with Langerin.
CURRENT MEDICINAL CHEMISTRY
(2022)
Book Review
Humanities, Multidisciplinary
Jose Luis Castro de Paz
SIGNA-REVISTA DE LA ASOCIACION ESPANOLA DE SEMIOTICA
(2022)
Article
Chemistry, Medicinal
Jonathan R. Miles, Xu Wang, Jose L. de Paz, Pedro M. Nieto
Summary: This study investigates the interaction between chondroitin sulfate and pleiotrophin, and finds that a synthetic tetrasaccharide is sufficient for full interaction with pleiotrophin. It is also hypothesized that the central region of pleiotrophin is intrinsically disordered and may undergo changes in properties upon binding.
Article
Art
Imanol Zumalde, Jose Luis Castro de Paz
Summary: This article examines the role played by the forest motif in Basque and Galician cinematography. Through a selection of contemporary films, the meaning of the forest and tree in each film is explored, followed by a comparative study on how these films update the symbolic heritage of the forest motif in the traditional imaginary of the region.
QUINTANA-REVISTA DO DEPARTAMENTO DE HISTORIA DA ARTE
(2022)
Article
Chemistry, Multidisciplinary
Serge Perez, Olga Makshakova, Jesus Angulo, Emiliano Bedini, Antonella Bisio, Jose Luis de Paz, Elisa Fadda, Marco Guerrini, Michal Hricovini, Milos Hricovini, Frederique Lisacek, Pedro M. Nieto, Kevin Pagel, Giulia Pairardi, Ralf Richter, Sergey A. . Samsonov, Romain A. . Vives, Dragana Nikitovic, Sylvie Ricard Blum
Summary: Glycosaminoglycans (GAGs) are complex poly-saccharides with diverse structures that play various functions in the extracellular matrix, cell surface, and nucleus. The chemical groups attached to GAGs form glycocodes that are still not fully understood. The influence of the structure and functions of proteoglycan core proteins on sulfated GAGs and vice versa needs further investigation. The lack of bioinformatic tools for mining GAG data sets limits our understanding of their structures and functions.
Article
Chemistry, Multidisciplinary
Irene Herrera-Gonzalez, Manuel Gonzalez-Cuesta, Michel Thepaut, Eugenie Laigre, David Goyard, Javier Rojo, Jose M. Garcia Fernandez, Franck Fieschi, Olivier Renaudet, Pedro M. Nieto, Carmen Ortiz Mellet
Summary: The carbohydrate chemical mimicry of sp2-iminosugars enables the synthesis of high-mannose-type oligosaccharide analogs whose solution and lectin bound conformations match those of the natural partners. The ability to finely modulate the binding affinity and selectivity towards specific lectins by incorporating the OMJ sp2-iminosugar caps offers potential applications in various fields.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Art
Jose Luis Castro de Paz, Hector Paz Otero
Summary: Since the end of the last century, a new historiography based on film analysis has reevaluated Republican Spanish cinema and that of the first post-war decades. This trend provides a starting point to delve into the trajectories of two notable Hispanic cinema masters, Luis Garcia Berlanga and Fernando Fernan Gomez. The article analyzes their career paths from comic to grotesque, with a focus on the literary works of Carlos Arniches and Wenceslao Fernandez Florez, which contribute to a realistic portrayal of the struggles and backwardness of Spanish society.
FOTOCINEMA-REVISTA CIENTIFICA DE CINE Y FOTOGRAFIA
(2023)
Article
Chemistry, Multidisciplinary
Irene Herrera-Gonzalez, Michel Thepaut, Elena M. Sanchez-Fernandez, Antonio di Maio, Corinne Vives, Javier Rojo, Jose M. Garcia Fernandez, Franck Fieschi, Pedro M. Nieto, Carmen Ortiz Mellet
Summary: Selective targeting of DC-SIGN and langerin may lead to the development of anti-infective agents. This study demonstrates that multivalent sp(2)-iminosugar-containing mannobioside analogs can achieve total DC-SIGN selectivity by leveraging the canonical binding mode towards high-mannose oligosaccharide ligands, acting as real biomimics.
CHEMICAL COMMUNICATIONS
(2022)
Article
Film, Radio, Television
Jose Luis Castro de Paz, Hector Paz Otero
Summary: Correspondence cinema, with its flaws and amateurish quality, is able to trigger strong memories of home and the past for emigrants. Through the subjective gaze of the spectator and the filmmaking techniques used, these films capture local landscapes, faces, rituals, and work, creating a powerful evocative experience.
ATALANTE-REVISTA DE ESTUDIOS CINEMATOGRAFICOS
(2022)