Impact of Specific N-Glycan Modifications on the Use of Plant-Produced SARS-CoV-2 Antigens in Serological Assays

N- and O-Glycosylation of the SARS-CoV-2 Spike Protein

(2021) Miloslav Sanda et al.   ANALYTICAL CHEMISTRY

Generation of enzymatically competent SARS‐CoV‐2 decoy receptor ACE2‐Fc in glycoengineered Nicotiana benthamiana

(2021) Alexandra Castilho et al.   Biotechnology Journal

Inactivation of N-Acetylglucosaminyltransferase I and α1,3-Fucosyltransferase Genes in Nicotiana tabacum BY-2 Cells Results in Glycoproteins With Highly Homogeneous, High-Mannose N-Glycans

(2021) Xavier Herman et al.   Frontiers in Plant Science

Carbohydrate Epitopes Currently Recognized as Targets for IgE Antibodies

(2021) Thomas A. Platts‐Mills et al.   ALLERGY

Synthetic Homogeneous Glycoforms of the SARS‐CoV‐2 Spike Receptor‐Binding Domain Reveals Different Binding Profiles of Monoclonal Antibodies

(2021) Ping Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Production of high‐quality SARS‐CoV‐2 antigens: impact of bioprocess and storage on glycosylation, biophysical attributes, and ELISA serologic tests performance

(2021) Rute Castro et al.   BIOTECHNOLOGY AND BIOENGINEERING

Nanoluciferase complementation-based bioreporter reveals the importance of N-linked glycosylation of SARS-CoV-2 S for viral entry

(2021) Taha Azad et al.   MOLECULAR THERAPY

Lewis A Glycans Are Present on Proteins Involved in Cell Wall Biosynthesis and Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions

(2021) Gernot Beihammer et al.   Frontiers in Plant Science

SARS-CoV-2 Antigens Expressed in Plants Detect Antibody Responses in COVID-19 Patients

(2021) Mohau S. Makatsa et al.   Frontiers in Plant Science

A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests

(2021) Miriam Klausberger et al.   EBioMedicine

Site-Specific Steric Control of SARS-CoV-2 Spike Glycosylation

(2021) Joel D. Allen et al.   BIOCHEMISTRY

Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants

(2021) Naphatsamon Uthailak et al.   Frontiers in Plant Science

N-Glycosylation of the SARS-CoV-2 Receptor Binding Domain Is Important for Functional Expression in Plants

(2021) Yun-Ji Shin et al.   Frontiers in Plant Science

Glycosylated Biotherapeutics: Immunological Effects of N-Glycolylneuraminic Acid

(2020) Sharon Yehuda et al.   Frontiers in Immunology

Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody

(2020) Dora Pinto et al.   NATURE

A serological assay to detect SARS-CoV-2 seroconversion in humans

(2020) Fatima Amanat et al.   NATURE MEDICINE

Site-specific glycan analysis of the SARS-CoV-2 spike

(2020) Yasunori Watanabe et al.   SCIENCE

Potential Applications of Plant Biotechnology against SARS-CoV-2

(2020) Teresa Capell et al.   TRENDS IN PLANT SCIENCE

Longitudinal Isolation of Potent Near-Germline SARS-CoV-2-Neutralizing Antibodies from COVID-19 Patients

(2020) Christoph Kreer et al.   CELL

Individuals with IgE antibodies to α‐Gal and CCD show specific IgG subclass responses different from subjects non‐sensitized to oligosaccharides

(2020) Patricia Román‐Carrasco et al.   CLINICAL AND EXPERIMENTAL ALLERGY

A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity

(2020) Jingyun Yang et al.   NATURE

Potent neutralizing antibodies against multiple epitopes on SARS-CoV-2 spike

(2020) Lihong Liu et al.   NATURE

A SARS-CoV-2 surrogate virus neutralization test based on antibody-mediated blockage of ACE2–spike protein–protein interaction

(2020) Chee Wah Tan et al.   NATURE BIOTECHNOLOGY

Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor

(2020) Peng Zhao et al.   Cell Host & Microbe

Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins

(2020) Yong Zhang et al.   MOLECULAR & CELLULAR PROTEOMICS

Rapid production of SARS-CoV-2 receptor binding domain (RBD) and spike specific monoclonal antibody CR3022 in Nicotiana benthamiana

(2020) Kaewta Rattanapisit et al.   Scientific Reports

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

(2020) Borja Diego-Martin et al.   Frontiers in Plant Science

In Vivo Glycan Engineering via the Mannosidase I Inhibitor (Kifunensine) Improves Efficacy of Rituximab Manufactured in Nicotiana benthamiana Plants

(2019) Vally Kommineni et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Distinct Fc alpha receptor N-glycans modulate the binding affinity to immunoglobulin A (IgA) antibodies

(2019) Kathrin Göritzer et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

CRISPR/Cas9-mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β-1,2-xylose and core α-1,3-fucose

(2018) Julia Jansing et al.   PLANT BIOTECHNOLOGY JOURNAL

Glycan shield and fusion activation of a deltacoronavirus spike glycoprotein fine-tuned for enteric infections

(2017) Xiaoli Xiong et al.   JOURNAL OF VIROLOGY

Establishment of a tobacco BY2 cell line devoid of plant-specific xylose and fucose as a platform for the production of biotherapeutic proteins

(2017) Uri Hanania et al.   PLANT BIOTECHNOLOGY JOURNAL

Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans

(2017) Sébastien Mercx et al.   Frontiers in Plant Science

Immunogenicity of glycans on biotherapeutic drugs produced in plant expression systems—The taliglucerase alfa story

(2017) Bonita Rup et al.   PLoS One

Using glyco-engineering to produce therapeutic proteins

(2015) Martina Dicker et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Plant Molecular Pharming for the Treatment of Chronic and Infectious Diseases

(2014) Eva Stoger et al.   Annual Review of Plant Biology

The choice of mammalian cell host and possibilities for glycosylation engineering

(2014) Michael Butler et al.   CURRENT OPINION IN BIOTECHNOLOGY

Inhibition of IgE binding to cross-reactive carbohydrate determinants enhances diagnostic selectivity

(2013) F. Holzweber et al.   ALLERGY

Generation of Biologically Active Multi-Sialylated Recombinant Human EPOFc in Plants

(2013) Alexandra Castilho et al.   PLoS One

Moss-based production of asialo-erythropoietin devoid of Lewis A and other plant-typical carbohydrate determinants

(2012) Juliana Parsons et al.   PLANT BIOTECHNOLOGY JOURNAL

N-Glycosylation engineering of plants for the biosynthesis of glycoproteins with bisected and branched complex N-glycans

(2011) Alexandra Castilho et al.   GLYCOBIOLOGY

Reduced Immunogenicity ofArabidopsis hgl1MutantN-Glycans Caused by Altered Accessibility of Xylose andcoreFucose Epitopes

(2011) Heidi Kaulfürst-Soboll et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

β-N-Acetylhexosaminidases HEXO1 and HEXO3 Are Responsible for the Formation of PaucimannosidicN-Glycans inArabidopsis thaliana

(2011) Eva Liebminger et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure

(2008) Richard Strasser et al.   PLANT BIOTECHNOLOGY JOURNAL