Article
Biochemistry & Molecular Biology
Emilija Brdaric, Dusanka Popovic, Svetlana Sokovic Bajic, Dina Tucovic, Jelena Mutic, Maja Cakic-Milosevic, Sladana Durdic, Maja Tolinacki, Aleksandra Popov Aleksandrov, Natasa Golic, Ivana Mirkov, Milica Zivkovic
Summary: Through experiments on rats, it was found that food supplemented with EPS-AN8 can reduce the levels of cadmium in the kidneys, liver, and blood, while enriching it in the feces. In addition, EPS-AN8 can reverse the effects of cadmium on oxidative stress, inflammation, and gut microbiota. Therefore, EPS-AN8 may be extremely significant in combating this toxic environmental pollutant.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Xingtao Zhou, Duoduo Zhang, Wucheng Qi, Tao Hong, Tao Xiong, Tingqin Wu, Fang Geng, Mingyong Xie, Shaoping Nie
Summary: EPS116 promotes intestinal epithelial regeneration by increasing the number of intestinal stem cells and modulating the gut microbiota profile, thereby maintaining intestinal barrier and homeostasis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Microbiology
Yi Zhou, Ling Duan, Yan Zeng, Xu Song, Kangcheng Pan, Lili Niu, Yang Pu, Jiakun Li, Abdul Khalique, Jing Fang, Bo Jing, Dong Zeng, Bairong Shen, Xueqin Ni
Summary: The intestinal microbiota of captive pandas, characterized by a high abundance of Enterobacteriaceae, has led to intestinal infection. Probiotic supplement Lactiplantibacillus plantarum BSG201683 (L. plantarum G83) has shown effectiveness in improving intestinal barrier function and inflammation. In vitro studies demonstrated that L. plantarum G83 reduced paracellular permeability, decreased proinflammatory cytokines, and enhanced anti-inflammatory responses through Toll-like receptor 4/ nuclear factor kappa-B (TLR4/NF-?B) signaling pathways.
Article
Food Science & Technology
Zheng Cao, Jinsong Gao, Wanyue Huang, Juli Yan, Anshan Shan, Xiang Gao
Summary: Curcumin protects against Deoxynivalenol-induced disruption of intestinal epithelial barrier by regulating apoptotic pathway and TJs pathway.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Nutrition & Dietetics
Longyan Li, Lei Wu, Tong Jiang, Tingting Liang, Lingshuang Yang, Ying Li, He Gao, Jumei Zhang, Xinqiang Xie, Qingping Wu
Summary: Sleep deprivation can result in intestinal diseases, and L. plantarum 124, a probiotic, has been found to attenuate intestinal damage caused by sleep deprivation. This is achieved by modulating the gut microbiota to restore intestinal health.
Review
Food Science & Technology
Jie Xiong, Dong-mei Liu, Yan-yan Huang
Summary: This review provides an overview of the isolation, purification, characterization, and yield optimization of exopolysaccharides from Lactiplantibacillus plantarum. The structural characterization and bioactivities of these exopolysaccharides are described, and the structure-bioactivity relationship is discussed. Additionally, the biosynthetic pathways of exopolysaccharides in Lactiplantibacillus plantarum are reviewed, considering the potential of synthetic biology in producing bioactive compounds.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jarukan Lamubol, Nobuaki Ohto, Hiroshige Kuwahara, Masashi Mizuno
Summary: In this study, the anti-inflammatory effects of LP22A3 on DSS-induced colitis were evaluated, and it was found that LP22A3 administration led to increased production of TGF-beta and IL-10, as well as an increase in CD103(+) DCs and Foxp3(+) Treg cells in the small intestine. These cells secreted high levels of anti-inflammatory cytokines, contributing to protection against inflammation. Overall, oral administration of LP22A3 may serve as an alternative therapeutic strategy for inflammatory bowel disease.
Article
Food Science & Technology
Bo Peng, Qingyu Cui, Chao Ma, Huaxi Yi, Pimin Gong, Kai Lin, Tongjie Liu, Lanwei Zhang
Summary: Probiotics, specifically Lactiplantibacillus plantarum YZX28, have shown protective effects on intestinal barrier dysfunction caused by enterotoxigenic Escherichia coli K80 (ETEC K80), and may serve as potential therapeutic agents for intestinal-related problems.
Article
Microbiology
Yuqi Gao, Mingze Niu, Xiaohui Yu, Tingting Bao, Zhaowei Wu, Xin Zhao
Summary: A newly identified EPS-synthetic gene cluster in Lactiplantibacillus plantarum plays a crucial role in enhancing biofilm formation ability, tolerance to harsh environmental stresses, adhesion to intestinal epithelial cells, as well as attenuating inflammation and inhibiting pathogenic bacteria adhesion. This finding highlights the significance of mobile EPS-synthetic elements in the probiotic activity of L. plantarum and provides insights for probiotic bacteria engineering.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Zifei Kan, Shujuan Zhang, Guisong Liao, Zheng Niu, Xiangyang Liu, Zhiwei Sun, Xia Hu, Yiling Zhang, Shasha Xu, Jingyi Zhang, Hong Zou, Xingcui Zhang, Zhenhui Song
Summary: Porcine epidemic diarrhea virus (PEDV) infection poses a serious threat to the economic development of pig farming. However, probiotics show potential therapeutic value in inhibiting PEDV proliferation by modulating Ca2+.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xuefang Zhao, Qi Liang
Summary: This study optimized the production of exopolysaccharides (EPS) from Lactiplantibacillus plantarum MC5 (Lp. plantarum MC5), evaluated its resistance to digestive juices, antioxidant activity, and rheological properties. The results showed that the maximum EPS production was achieved under specific conditions. The EPS-MC5 exhibited high resistance to digestive enzymes and antioxidant activity, as well as favorable rheological properties. These findings contribute to the understanding and potential applications of EPS in human health and functional foods.
Article
Microbiology
Liansheng Yu, Guangbin Ye, Xintong Qi, Yi Yang, Bosen Zhou, Yunye Zhang, Renpeng Du, Jingping Ge, Wenxiang Ping
Summary: In this study, an EPS-producing strain of Lactiplantibacillus plantarum HDC-01 was isolated from sauerkraut, and the structure, properties, and biological activity of the EPS were analyzed. The EPS had a molecular weight of 2.505 x 10(6) Da and was composed of glucose/glucopyranose subunits connected by α-(1→6) glycosidic bonds. It exhibited thermal stability, water holding capacity, and milk coagulation ability, and also promoted the growth of probiotics. Therefore, L. plantarum EPS shows potential as a prebiotic in the food and medicine industries.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Medicinal
Xiaozhen Diao, Katsuhisa Yamada, Yuji Shibata, Chiaki Imada
Summary: This study found that the metabolites produced by a Lactiplantibacillus plantarum strain BF1-13 isolated from deep seawater have a protective effect on the intestinal epithelial barrier function. The metabolites enhance the expression of the tight junction protein Claudin-4 and suppress the expression of aquaporin3. Lactic acid is one of the components in the metabolites that contribute to the enhancement of tight junctions and the reduction of hydrogen peroxide invasion into intestinal cells.
Article
Biochemistry & Molecular Biology
Lei Tian, Yu Liu, Xiaofang Xu, Pengrui Jiao, Gaofei Hu, Yaqian Cui, Jixiang Chen, Yucheng Ma, Xi Jin, Kunjie Wang, Qun Sun
Summary: This study investigated the effect of Lactiplantibacillus plantarum J-15 on kidney stone formation and found that it reduces the formation of renal crystallization and urinary oxalic acid. It restores intestinal microflora and metabolic disorder, protects intestinal barrier function, and alleviates inflammation, suggesting that it could be a potential therapy for renal stones.
Article
Biotechnology & Applied Microbiology
Wei Liang, Yansong Gao, Yujuan Zhao, Lei Gao, Zijian Zhao, Zhongmei He, Shengyu Li
Summary: This study demonstrated the preventive effect of Lactiplantibacillus plantarum ELF051 on antibiotic-associated diarrhea, as well as its modulation of gut microbiota and suppression of inflammatory response.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Pharmacology & Pharmacy
Miodrag Colic, Marina Bekic, Sergej Tomic, Jelena Dokic, Dusan Radojevic, Katarina Savikin, Natasa Miljus, Milan Markovic, Ranko Skrbic
Summary: Pomegranate peel extract (PoPEx) has complex immunoregulatory effects on T cells, with antioxidant and anti-inflammatory properties, which could assist in the suppression of chronic inflammatory and autoimmune diseases.
Article
Biochemistry & Molecular Biology
Jovana Jagodic, Sladan Pavlovic, Slavica Borkovic-Mitic, Milan Perovic, Zeljko Mikovic, Sladana Durdic, Dragan Manojlovic, Aleksandar Stojsavljevic
Summary: This study investigated the levels of toxic and essential trace metals in the umbilical cord sera, maternal sera, and placental tissue samples of healthy pregnant women. It was found that all trace metals can cross the placental barrier and reach the fetus. Statistical analysis revealed significant differences in levels of toxic and essential trace metals between different clinical samples. Principal component analysis highlighted the most influential trace metals in distinguishing placenta from maternal and umbilical cord serum samples. Overall, these findings contribute to our understanding of transplacental transfer of trace metals and metal exposure in healthy pregnant women and their fetuses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Milos Mitrovic, Verica Stankovic-Popovic, Maja Tolinacki, Natasa Golic, Svetlana Sokovic Bajic, Katarina Veljovic, Branislav Nastasijevic, Ivan Soldatovic, Petar Svorcan, Nada Dimkovic
Summary: This study aimed to assess the efficacy and safety of altering dysbiotic gut flora through synbiotic supplementation in reducing gut-derived uremic toxins and inflammation. The results showed that synbiotic treatment significantly modified the gut microbiome, reduced serum levels of indoxyl sulfate, improved estimated glomerular filtration rate, and decreased high-sensitivity C-reactive protein levels in treated patients. The treatment was found to be safe and effective.
JOURNAL OF RENAL NUTRITION
(2023)
Article
Chemistry, Analytical
Sladana Durdic, Milos Ognjanovic, Maja Krstic Ristivojevic, Bratislav Antic, Tanja Cirkovic Velickovic, Jelena Mutic, Zoltan Konya, Dalibor Stankovic
Summary: In this study, an electrochemical approach based on multiwall carbon nanotubes and neodymium hydroxide nanoflakes was developed for the detection of bovine serum albumin. The method showed a linear voltammetric response and exhibited low detection limit and high precision. The approach also demonstrated satisfactory selectivity towards interfering compounds and showed potential for application in cow milk samples.
Article
Cell Biology
Tobias Tertel, Sergej Tomic, Jelena Dokic, Dusan Radojevic, Dejan Stevanovic, Natasa Ilic, Bernd Giebel, Maja Kosanovic
Summary: This study aimed to explore the characteristics of serum extracellular vesicles (EVs) in COVID-19 patients and investigate their potential as biomarkers. The study found that severe COVID-19 patients had increased levels of CD13(+) and CD82(+) EVs in their serum, while mild COVID-19 patients had increased levels of HLA-ABC(+) EVs and CD24(+) EVs. The abundance of these EVs was correlated with disease severity, cytokine concentration, and immunopathogenesis.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Gastroenterology & Hepatology
Dusan Radojevic, Marina Bekic, Alisa Gruden-Movsesijan, Natasa Ilic, Miroslav Dinic, Aleksandar Bisenic, Natasa Golic, Dragana Vucevic, Jelena Dokic, Sergej Tomic
Summary: This study shows that activated myeloid-derived suppressor cells (MDSCs), especially those differentiated with prostaglandin E2 (MDSC-PGE2), can effectively ameliorate symptoms in experimental autoimmune encephalomyelitis (EAE) and prevent extensive changes in gut microbiota composition. The findings suggest the therapeutic potential of MDSCs in the acute phases of multiple sclerosis (MS) and the importance of gut microbiota in MS therapy.
Article
Biochemistry & Molecular Biology
Aleksandar Stojsavljevic, Nebojsa Zecevic, Mladen Mihailovic, Jovana Jagodic, Sladana Durdic, Milan Perovic, Dragan Manojlovic
Summary: This study aimed to determine the levels of 39 (ultra)trace elements and 5 macroelements in human semen samples with confirmed normozoospermia using ICP-based techniques. The findings indicated that Zn is the most prominent and Tm is the least prominent among the analyzed (ultra)trace elements in semen samples.
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2022)
Article
Chemistry, Analytical
Aleksandar Mijajlovic, Milos Ognjanovic, Dragan Manojlovic, Filip Vlahovic, Sladana Durdic, Vesna Stankovic, Dalibor Stankovic
Summary: There are 10 million people with Parkinson's disease worldwide. The most effective medication for the disease is levodopa (L-DOPA), but long-term consumption leads to side effects, necessitating the monitoring of its concentrations. This study developed a new electrochemical sensor using Eu2O3@Cr2O3 composite nanoparticles to detect L-DOPA.
Article
Chemistry, Analytical
Sladana Durdic, Filip Vlahovic, Milan Markicevic, Jelena Mutic, Dragan Manojlovic, Vesna Stankovic, L'ubomir Svorc, Dalibor Stankovic
Summary: A screen-printed diamond electrode coupled with a point-of-care platform was used for the electrochemical determination of pterostilbene. The oxidation of pterostilbene was found to be pH-dependent, and the most probable reactive site of the analyte was determined using DFT-based reactivity descriptors. The optimized differential pulse voltammetry method showed excellent selectivity and nanomolar-level detection limits for pterostilbene.
Article
Biochemistry & Molecular Biology
Emilija Brdaric, Dusanka Popovic, Svetlana Sokovic Bajic, Dina Tucovic, Jelena Mutic, Maja Cakic-Milosevic, Sladana Durdic, Maja Tolinacki, Aleksandra Popov Aleksandrov, Natasa Golic, Ivana Mirkov, Milica Zivkovic
Summary: Through experiments on rats, it was found that food supplemented with EPS-AN8 can reduce the levels of cadmium in the kidneys, liver, and blood, while enriching it in the feces. In addition, EPS-AN8 can reverse the effects of cadmium on oxidative stress, inflammation, and gut microbiota. Therefore, EPS-AN8 may be extremely significant in combating this toxic environmental pollutant.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Nina Tomic, Tamara Matic, Nenad Filipovic, Dragana Mitic Culafic, Aldo R. Boccacccini, Magdalena M. Stevanovic
Summary: Recent studies have shown that resveratrol, a polyphenolic compound, has various beneficial effects on human health including cardioprotection, neuroprotection, anti-cancer, anti-inflammatory, osteoinductive, and antimicrobial effects. However, its poor water solubility, sensitivity to oxygen, light, heat, rapid metabolism, and low bioavailability limit its potential for in vivo applications. The synthesis of resveratrol in nanoparticle form could be a possible solution to overcome these limitations.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2023)
Article
Polymer Science
Michaela Pavlickova, Sladana Durdic, Michal Hatala, Dalibor Stankovic, Lubomir Svorc, Peter Veteska, Pavol Gemeiner
Summary: This study demonstrates the preparation of pad-printable inks containing reduced graphene oxide (rGO) and cellulose-based polymers (ethylcellulose and cellulose acetate butyrate) for pad printing. The most suitable polymer concentrations for pad printing are determined based on rheological parameters and printability analysis. The resulting rGO electrodes, printed with ethylcellulose as a binder, show good performance in detecting ascorbic acid.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Miroslav Dinic, Stefan Jakovljevic, Nikola Popovic, Dusan Radojevic, Katarina Veljovic, Natasa Golic, Amarela Terzic-Vidojevic
Summary: This study investigated the probiotic features of two strains (S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21) isolated from artisanal sour milk and yogurt, and demonstrated their ability to upregulate autophagy-related genes and immune defense genes in Caenorhabditis elegans. The study also revealed that yogurt prepared with these strains is rich in vitamin B2 and dominant amino acids known for their pro-longevity properties. These findings highlight the potential of these starter cultures and yogurt as fermented foods with added-value properties.
JOURNAL OF DAIRY SCIENCE
(2023)