CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l-proline exporter for l-proline hyperproduction
出版年份 2022 全文链接
标题
CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l-proline exporter for l-proline hyperproduction
作者
关键词
-
出版物
Nature Communications
Volume 13, Issue 1, Pages -
出版商
Springer Science and Business Media LLC
发表日期
2022-02-16
DOI
10.1038/s41467-022-28501-7
参考文献
相关参考文献
注意:仅列出部分参考文献,下载原文获取全部文献信息。- Advances in metabolic engineering of Corynebacterium glutamicum to produce high-value active ingredients for food, feed, human health, and well-being
- (2021) Sabrina Wolf et al. Essays in Biochemistry
- Cascaded valorization of brown seaweed to produce l-lysine and value-added products using Corynebacterium glutamicum streamlined by systems metabolic engineering
- (2021) Sarah Lisa Hoffmann et al. METABOLIC ENGINEERING
- Metabolic engineering of carbohydrate metabolism systems in Corynebacterium glutamicum for improving the efficiency of l-lysine production from mixed sugar
- (2020) Jian-Zhong Xu et al. Microbial Cell Factories
- Significantly enhancing production of trans-4-hydroxy-l-proline by integrated system engineering in Escherichia coli
- (2020) Mengfei Long et al. Science Advances
- Efficient Multiplex Gene Repression by CRISPR-dCpf1 in Corynebacterium glutamicum
- (2020) Mingyue Li et al. Frontiers in Bioengineering and Biotechnology
- De Novo Engineering of Corynebacterium glutamicum for l-Proline Production
- (2020) Jiao Zhang et al. ACS Synthetic Biology
- Fermentative N-Methylanthranilate Production by Engineered Corynebacterium glutamicum
- (2020) Tatjana Walter et al. Microorganisms
- Multiplex gene editing and large DNA fragment deletion by the CRISPR/Cpf1-RecE/T system in Corynebacterium glutamicum
- (2020) Nannan Zhao et al. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
- Isoleucyl-tRNA synthetase mutant based whole-cell biosensor for high-throughput selection of isoleucine overproducers
- (2020) Xue Sun et al. BIOSENSORS & BIOELECTRONICS
- CRISPR-derived genome editing technologies for metabolic engineering
- (2020) Keiji Nishida et al. METABOLIC ENGINEERING
- Glutaric acid production by systems metabolic engineering of an l-lysine–overproducingCorynebacterium glutamicum
- (2020) Taehee Han et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Systems Metabolic Engineering Strategies: Integrating Systems and Synthetic Biology with Metabolic Engineering
- (2019) Kyeong Rok Choi et al. TRENDS IN BIOTECHNOLOGY
- Optimizing a CRISPR-Cpf1-based genome engineering system for Corynebacterium glutamicum
- (2019) Jiao Zhang et al. Microbial Cell Factories
- Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping
- (2019) Jiazhang Lian et al. Nature Communications
- CRISPR/Cas12a Mediated Genome Editing To Introduce Amino Acid Substitutions into the Mechanosensitive Channel MscCG of Corynebacterium glutamicum
- (2019) Karin Krumbach et al. ACS Synthetic Biology
- Beyond the semi-synthetic artemisinin: metabolic engineering of plant-derived anti-cancer drugs
- (2019) Ines Carqueijeiro et al. CURRENT OPINION IN BIOTECHNOLOGY
- MACBETH: Multiplex automated Corynebacterium glutamicum base editing method
- (2018) Yu Wang et al. METABOLIC ENGINEERING
- Metabolically engineered Corynebacterium glutamicum for bio-based production of chemicals, fuels, materials, and healthcare products
- (2018) Judith Becker et al. METABOLIC ENGINEERING
- A RecET-assisted CRISPR–Cas9 genome editing in Corynebacterium glutamicum
- (2018) Bo Wang et al. Microbial Cell Factories
- Integrated design, execution, and analysis of arrayed and pooled CRISPR genome-editing experiments
- (2018) Matthew C Canver et al. Nature Protocols
- Recombinant Protein Expression System in Corynebacterium glutamicum and Its Application
- (2018) Min Ju Lee et al. Frontiers in Microbiology
- Switch of metabolic status: redirecting metabolic flux for acetoin production from glycerol by activating a silent glycerol catabolism pathway
- (2017) Yu Wang et al. METABOLIC ENGINEERING
- Metabolic engineering of Corynebacterium glutamicum for the production of 3-hydroxypropionic acid from glucose and xylose
- (2017) Zhen Chen et al. METABOLIC ENGINEERING
- CRISPR/Cas9-coupled recombineering for metabolic engineering of Corynebacterium glutamicum
- (2017) Jae Sung Cho et al. METABOLIC ENGINEERING
- Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum
- (2017) Jiao Liu et al. Microbial Cell Factories
- Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
- (2017) Feng Peng et al. Microbial Cell Factories
- High‐throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniae
- (2017) Xue Liu et al. Molecular Systems Biology
- Sort-Seq Approach to Engineering a Formaldehyde-Inducible Promoter for Dynamically Regulated Escherichia coli Growth on Methanol
- (2017) Julia Rohlhill et al. ACS Synthetic Biology
- A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria
- (2016) Jason M. Peters et al. CELL
- Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems
- (2016) Ryan T. Leenay et al. MOLECULAR CELL
- Rewriting yeast central carbon metabolism for industrial isoprenoid production
- (2016) Adam L. Meadows et al. NATURE
- Consequences of Cas9 cleavage in the chromosome ofEscherichia coli
- (2016) Lun Cui et al. NUCLEIC ACIDS RESEARCH
- TransportDB 2.0: a database for exploring membrane transporters in sequenced genomes from all domains of life
- (2016) Liam D. H. Elbourne et al. NUCLEIC ACIDS RESEARCH
- Metabolic Engineering of an ATP-Neutral Embden-Meyerhof-Parnas Pathway in Corynebacterium glutamicum: Growth Restoration by an Adaptive Point Mutation in NADH Dehydrogenase
- (2015) Gajendar Komati Reddy et al. APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- Improving the electro-transformation efficiency of Corynebacterium glutamicum by weakening its cell wall and increasing the cytoplasmic membrane fluidity
- (2015) Yili Ruan et al. BIOTECHNOLOGY LETTERS
- The many roles of glutamate in metabolism
- (2015) Mark C. Walker et al. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
- Systems strategies for developing industrial microbial strains
- (2015) Sang Yup Lee et al. NATURE BIOTECHNOLOGY
- Identification of a plastidial phenylalanine exporter that influences flux distribution through the phenylalanine biosynthetic network
- (2015) Joshua R. Widhalm et al. Nature Communications
- Microbial production of amino acids and derived chemicals: Synthetic biology approaches to strain development
- (2014) Volker F Wendisch CURRENT OPINION IN BIOTECHNOLOGY
- sgRNAcas9: A Software Package for Designing CRISPR sgRNA and Evaluating Potential Off-Target Cleavage Sites
- (2014) Shengsong Xie et al. PLoS One
- Comprehensive analysis of the Corynebacterium glutamicum transcriptome using an improved RNAseq technique
- (2013) Katharina Pfeifer-Sancar et al. BMC GENOMICS
- COBRApy: COnstraints-Based Reconstruction and Analysis for Python
- (2013) Ali Ebrahim et al. BMC Systems Biology
- Precise and reliable gene expression via standard transcription and translation initiation elements
- (2013) Vivek K Mutalik et al. NATURE METHODS
- Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay
- (2013) Michael E. Lee et al. NUCLEIC ACIDS RESEARCH
- Recombineering in Corynebacterium glutamicum combined with optical nanosensors: a general strategy for fast producer strain generation
- (2013) Stephan Binder et al. NUCLEIC ACIDS RESEARCH
- Multiplex Genome Engineering Using CRISPR/Cas Systems
- (2013) L. Cong et al. SCIENCE
- Sugar transport systems in Corynebacterium glutamicum: features and applications to strain development
- (2012) Masato Ikeda APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
- RNA processing enables predictable programming of gene expression
- (2012) Lei Qi et al. NATURE BIOTECHNOLOGY
- Systems metabolic engineering of microorganisms for natural and non-natural chemicals
- (2012) Jeong Wook Lee et al. Nature Chemical Biology
- Rapid Reaction Kinetics of Proline Dehydrogenase in the Multifunctional Proline Utilization A Protein
- (2011) Michael A. Moxley et al. BIOCHEMISTRY
- Engineering of Corynebacterium glutamicum with an NADPH-Generating Glycolytic Pathway for L-Lysine Production
- (2010) S. Takeno et al. APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- The properties and contribution of the Corynebacterium glutamicum MscS variant to fine-tuning of osmotic adaptation
- (2010) Kirsten Börngen et al. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
- The Protein Encoded byNCgl1221inCorynebacterium glutamicumFunctions as a Mechanosensitive Channel
- (2010) Ken-ichi HASHIMOTO et al. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- Molecular Mechanisms Modulating Glutamate Kinase Activity. Identification of the Proline Feedback Inhibitor Binding Site
- (2010) Isabel Pérez-Arellano et al. JOURNAL OF MOLECULAR BIOLOGY
- What is flux balance analysis?
- (2010) Jeffrey D Orth et al. NATURE BIOTECHNOLOGY
- Coding-Sequence Determinants of Gene Expression in Escherichia coli
- (2009) G. Kudla et al. SCIENCE
Find Funding. Review Successful Grants.
Explore over 25,000 new funding opportunities and over 6,000,000 successful grants.
ExploreBecome a Peeref-certified reviewer
The Peeref Institute provides free reviewer training that teaches the core competencies of the academic peer review process.
Get Started