期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 74, 期 12, 页码 2546-2549出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.100636
关键词
Corynebacterium glutamicum; glutamate overproduction; mechanosensitive channel; electrophysiological study; patch-clamp method
类别
资金
- Ministry of Education, Culture, Sport, Science, and Technology of Japan (MEXT) [S0801023]
The function of the NCgl1221-encoded protein of Corynebacterium glutamicum was analyzed using Bacillus subtilis as host because a method for preparing the giant provacuole required for electrophysiological studies has been established. Expression of NCgl1221 in a strain deficient in mscL and ykuT, both of which encode mechanosensitive channels, resulted in an 8.9-fold higher cell survival rate upon osmotic downshock than the control. Electrophysiological investigation showed that the giant provacuole prepared from this strain, expressing NCgl1221, exhibited significantly higher pressure-dependent conductance than the control. These findings show that the NCgl1221-encoded protein functions as a mechanosensitive channel.
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