Using continuous directed evolution to improve enzymes for plant applications

The number of catalytic cycles in an enzyme’s lifetime and why it matters to metabolic engineering

(2021) Andrew D. Hanson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure and function of aerotolerant, multiple-turnover THI4 thiazole synthases

(2021) Jaya Joshi et al.   BIOCHEMICAL JOURNAL

Directed Evolution: Methodologies and Applications

(2021) Yajie Wang et al.   CHEMICAL REVIEWS

Rapid generation of potent antibodies by autonomous hypermutation in yeast

(2021) Alon Wellner et al.   Nature Chemical Biology

The Development of Herbicide Resistance Crop Plants Using CRISPR/Cas9-Mediated Gene Editing

(2021) Huirong Dong et al.   Genes

Systems for in vivo hypermutation: a quest for scale and depth in directed evolution

(2021) Gordon Rix et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Bioinformatic and experimental evidence for suicidal and catalytic plant THI4s

(2020) Jaya Joshi et al.   BIOCHEMICAL JOURNAL

The evolving landscape around genome editing in agriculture

(2020) Sarah M Schmidt et al.   EMBO REPORTS

Prime genome editing in rice and wheat

(2020) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

The developing toolkit of continuous directed evolution

(2020) Mary S. Morrison et al.   Nature Chemical Biology

Automated Continuous Evolution of Proteins in Vivo

(2020) Ziwei Zhong et al.   ACS Synthetic Biology

Targeted Diversification in the S. cerevisiae Genome with CRISPR-Guided DNA Polymerase I

(2020) Connor J. Tou et al.   ACS Synthetic Biology

Gene-specific mutagenesis enables rapid continuous evolution of enzymes in vivo

(2020) Hyojin Park et al.   NUCLEIC ACIDS RESEARCH

In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9

(2020) Beatriz Álvarez et al.   Nature Communications

Applications of protein engineering and directed evolution in plant research

(2019) Martin KM Engqvist et al.   PLANT PHYSIOLOGY

N-degron and C-degron pathways of protein degradation

(2019) Alexander Varshavsky   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Innovation by Evolution: Bringing New Chemistry to Life (Nobel Lecture)

(2019) Frances H. Arnold   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Processive Protein Chimera Introduces Mutations across Defined DNA Regions In Vivo

(2018) Christopher L. Moore et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Construction of synthetic T7 RNA polymerase expression systems

(2018) Shaunak Kar et al.   METHODS

Redesigning thiamin synthesis: Prospects and potential payoffs

(2018) Andrew D. Hanson et al.   PLANT SCIENCE

Daring metabolic designs for enhanced plant carbon fixation

(2018) Arren Bar-Even   PLANT SCIENCE

CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window

(2018) Shakked O. Halperin et al.   NATURE

Scalable, Continuous Evolution of Genes at Mutation Rates above Genomic Error Thresholds

(2018) Arjun Ravikumar et al.   CELL

Parts-prospecting For a High-efficiency Thiamin Thiazole Biosynthesis Pathway

(2018) Jiayi Sun et al.   PLANT PHYSIOLOGY

Tunable Expression Systems for Orthogonal DNA Replication

(2018) Ziwei Zhong et al.   ACS Synthetic Biology

Dissecting Redox Biology Using Fluorescent Protein Sensors

(2016) Markus Schwarzländer et al.   ANTIOXIDANTS & REDOX SIGNALING

Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases

(2016) Xuan Zhang et al.   BIOCHEMISTRY

What drives parallel evolution?

(2016) Susan F. Bailey et al.   BIOESSAYS

From Suicide Enzyme to Catalyst: The Iron-Dependent Sulfide Transfer in Methanococcus jannaschii Thiamin Thiazole Biosynthesis

(2016) Bekir E. Eser et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Does Abiotic Stress Cause Functional B Vitamin Deficiency in Plants?

(2016) Andrew D. Hanson et al.   PLANT PHYSIOLOGY

A synthetic pathway for the fixation of carbon dioxide in vitro

(2016) Thomas Schwander et al.   SCIENCE

The Yeast Deletion Collection: A Decade of Functional Genomics

(2014) Guri Giaever et al.   GENETICS

An orthogonal DNA replication system in yeast

(2014) Arjun Ravikumar et al.   Nature Chemical Biology

Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase

(2011) Abhishek Chatterjee et al.   NATURE

Removing allosteric feedback inhibition of tomato 4-coumarate:CoA ligase by directed evolution

(2011) Moti Alberstein et al.   PLANT JOURNAL

Chaperonins: two rings for folding

(2011) Hugo Yébenes et al.   TRENDS IN BIOCHEMICAL SCIENCES

BioNumbers—the database of key numbers in molecular and cell biology

(2009) Ron Milo et al.   NUCLEIC ACIDS RESEARCH

Advances in laboratory evolution of enzymes

(2008) Shimon Bershtein et al.   CURRENT OPINION IN CHEMICAL BIOLOGY