Review
Chemistry, Multidisciplinary
Faranak Mankavi, Rana Ibrahim, Hongjun Wang
Summary: This article summarizes the recent advances in nerve guidance conduits (NGCs) for peripheral nerve regeneration and repair, including biomaterial innovations, structural design, advanced fabrication technologies, and cellular responses to biomimetic cues.
Article
Engineering, Multidisciplinary
Renliang Zhao, Lianghua Jiang, Juan Du, Bitong Xu, Ang Li, Wei Wang, Shichang Zhao, Xiaolin Li
Summary: The biomimetic electrospun conduit Gel@PLGA/Col showed outstanding nerve regeneration performance in a sciatic nerve defect implantation model, inducing axon growth and facilitating nerve continuity recovery. This structure holds great potential for peripheral nerve repair and functional recovery after injury.
COMPOSITES PART B-ENGINEERING
(2022)
Review
Biotechnology & Applied Microbiology
Zhiwen Yan, Cheng Chen, Gonzalo Rosso, Yun Qian, Cunyi Fan
Summary: This review examines the role of two-dimensional nanomaterials in promoting peripheral nerve regeneration by facilitating angiogenesis and supporting new vessel formation. The potential of two-dimensional nanomaterials as a solution for long-range peripheral nerve defects is highlighted, with strategies aimed at addressing energy deficiency post-injury being proposed to enhance therapeutic effects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell & Tissue Engineering
Zheng Yang, Yang Yang, Yichi Xu, Weiqian Jiang, Yan Shao, Jiahua Xing, Youbai Chen, Yan Han
Summary: In this study, engineered exosomes were used as carriers to efficiently deliver NT-3 mRNA to recipient cells and promote nerve regeneration in a rat sciatic nerve defect model, demonstrating the potential for improving nerve regeneration.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Liwei Yan, Ali Entezari, Zhongpu Zhang, Jingxiao Zhong, Jing Liang, Qing Li, Jian Qi
Summary: This study used micro-CT to reconstruct a three-dimensional eECM model sourced from mixed nerves. The microstructural and biomechanical characteristics of eECM were analyzed experimentally and computationally. The findings showed that the eECM model had relatively low permeability and that key microstructural and biomechanical parameters played an important role in scaffold design.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Trevor Poitras, Douglas W. Zochodne
Summary: Common mechanisms of peripheral axon regeneration are recruited following diverse forms of damage to peripheral nerve axons. Supporting peripheral axon regrowth could be achieved through direct axon support or manipulation of the inflammatory milieu. Understanding the molecular barriers to regrowth and exploiting the downstream signaling pathways that impact neuron growth are promising approaches for promoting adult peripheral axon regeneration. This review emphasizes intrinsic aspects of adult peripheral axon regeneration and highlights several molecular barriers to regrowth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Lorena R. Lizarraga-Valderrama, Giulia Ronchi, Rinat Nigmatullin, Federica Fregnan, Pooja Basnett, Alexandra Paxinou, Stefano Geuna, Ipsita Roy
Summary: The study demonstrated that PHA-NGCs are highly promising in promoting peripheral nerve regeneration, showing comparable outcomes to autografts in functional evaluation and nerve morphology.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Gang Wang, Panjian Lu, Pingping Qiao, Ping Zhang, Xiaodong Cai, Leili Tang, Tianmei Qian, Hongkui Wang
Summary: Rapid and functional vascularization is a bottleneck in tissue engineering for regenerative medicine. Micro-CT scanning was used to depict the perfused vascular network reconstruction during peripheral nerve regeneration, revealing the stronger ability of autologous nerve for sprouting angiogenesis and vascular remodeling compared to tissue engineered nerve. Vascular remodeling was found to be crucial for nerve regeneration and its blockage inhibited axons and myelin sheaths regeneration.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Neurosciences
Leyang Li, Su Chen, Hiroyuki Yokoyama, Hidetoshi Kaburagi, Takashi Hirai, Kunikazu Tsuji, Mitsuhiro Enomoto, Yoshiaki Wakabayashi, Atsushi Okawa
Summary: Peripheral nerve injury (PNI) leads to severe functional loss in extremities, and delayed nerve repair results in muscle denervation and atrophy. To better understand NMJ degeneration and regeneration after PNI, we established two models of nerve repair in mice and compared their outcomes. Our results suggest that allogeneic nerve grafting is more effective than end-to-end neurorrhaphy in promoting functional recovery and increasing reinnervated NMJs and Schwann cells in the target muscle. Further investigation is needed to explore the relationship between NMJ and Schwann cells in the target muscle.
Review
Biochemistry & Molecular Biology
Dong Keon Yon, Yong Jun Kim, Dong Choon Park, Su Young Jung, Sung Soo Kim, Joon Hyung Yeo, Jeongmin Lee, Jae Min Lee, Seung Geun Yeo
Summary: A literature review on the role of autophagy in peripheral nerve injury and regeneration was conducted. The review identified several factors, such as LC3II/I, PI3K, mTOR, Beclin-1, ATG3, ATG5, ATG7, ATG9, and ATG12, that are involved in the process of nerve degeneration and regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Poornima Ramburrun, Pradeep Kumar, Elias Ndobe, Yahya E. Choonara
Summary: This study evaluated a biosimulated nerve repair system providing three types of regenerative cues in a peripheral nerve injury model. The nanofibers supported PC12 proliferation, preserved muscle mass, and gradually improved functional recovery. The combination of physical guidance, chemical cues, and therapeutic cues offers a promising strategy for the regeneration of severed peripheral nerves.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Luke Juckett, Tiam Mana Saffari, Benjamin Ormseth, Jenna-Lynn Senger, Amy M. M. Moore
Summary: Peripheral nerve injuries (PNI) often result in lifelong disability with rare complete functional recovery. Intraoperative electrical stimulation (ES) is a promising surgical adjunct that can augment the intrinsic molecular pathways of nerve regeneration. Clinical trials have shown that ES can be an efficacious therapy to improve patient outcomes following PNIs.
Article
Neurosciences
P. Komirishetty, K. Zubkow, A. Areti, H. Ong, D. W. Zochodne
Summary: The study found that local delivery of Rb1 siRNA can promote nerve regeneration at the outset, enhancing reinnervation of epidermal axons. Combining intrinsic Rb1 knockdown with exogenous insulin can significantly improve growth patterns of adult sensory neurons in vitro and enhance regeneration outcomes in vivo. Overall, the findings highlight the responsiveness of regrowing peripheral neurons to both intrinsic and exogenous approaches that improve their recovery, as well as a novel local approach to manipulate gene expression and outcome in regrowing axons.
NEUROBIOLOGY OF DISEASE
(2021)
Review
Cell Biology
Jingxin Zhang, Binjing Zhang, Jinglan Zhang, Weimin Lin, Shiwen Zhang
Summary: Magnesium as a suitable material for promoting peripheral nerve regeneration has both challenges and potentials, but there is currently limited research on this topic, requiring further exploration to address the shortcomings and challenges identified.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Neurosciences
Wenqian Zhao, Hui Tu, Jianxiao Chen, Jing Wang, Haoting Liu, Fengshou Zhang, Jing Li
Summary: Repairing injuries to the nervous system has always been a prominent topic in clinical research. With the emergence of tissue engineering, hydrogel materials have been identified as a promising technology for repairing nervous system injuries. By controlling their composition and structure, hydrogels can be functionalized and almost fully matched with nerve tissue, making them suitable for repairing injuries to both the central and peripheral nervous systems.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Engineering, Biomedical
Ruizhi Hu, Zhixin Chen, Chen Dai, Xiang Guo, Wei Feng, Zhuang Liu, Han Lin, Yu Chen, Rong Wu
Summary: The study presents a novel silicene-based silicene@Pt composite nanosheets that function as contrast agents for CT imaging and dual radio-sensitizing agents for radiotherapy through functionalization of Pt components, as well as realizing photonic tumor hyperthermia with strong photothermal-conversion performance. This approach broadens the biomedical applications of silicene and develops functionalization strategies for versatile biomedical applications.
Article
Chemistry, Multidisciplinary
Chuang Yang, Yao Luo, Han Lin, Min Ge, Jianlin Shi, Xianlong Zhang
Summary: 2D niobium carbide (Nb2C) MXene titanium plate Nb2C@TP)-based clinical implant offers practical multimodal anti-infection functions, capable of destroying biofilms for direct bacteria elimination through down-regulating bacterial energy metabolism pathways, suppressing biofilm formation, and enhancing biofilm detachment via activating the accessory gene regulator.
Review
Chemistry, Multidisciplinary
Xiangyu Lu, Shanshan Gao, Han Lin, Jianlin Shi
Summary: This article reviews the latest advances in nanocatalytic tumor therapy, focusing on the remarkable achievements of single-atom catalysts (SACs) in several different treatment modalities, as well as the attention on catalytic nanoplatform construction and catalytic mechanism. Challenges still remain for the future design and engineering of SACs for tumor therapy.
Article
Chemistry, Multidisciplinary
Ruizhi Hu, Chen Dai, Chunmei Wang, Jie Lin, Hui Hu, Zhifang Li, Han Lin, Li Ding, Yu Chen, Bo Zhang
Summary: By developing 2D arsenic-phosphorus (AsP) nanosheets, toxic arsenic is confined into AsP crystals, improving the biosafety and biocompatibility of arsenic-based chemotherapeutic drugs. AsP nanosheets exhibit high light absorption and strong photothermal-conversion efficiency in the second near infrared biowindow (NIR-II), enabling contrast-enhanced photoacoustic imaging and local tumor hyperthermia, leading to a synergistic arsenic-mediated antineoplastic effect.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Engineering, Biomedical
Wencheng Wu, Yinying Pu, Xiangyu Lu, Han Lin, Jianlin Shi
Summary: Nanocatalytic medicine is a promising strategy for cancer therapeutics that generates toxic agents within tumors through catalytic reactions. Transitional metal-based nanocatalytic medicines show significant catalytic activity and clinical potential, but face obstacles and challenges in design and clinical application.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Min Ge, Deliang Xu, Zhixin Chen, Chengyang Wei, Yanxia Zhang, Chuang Yang, Yu Chen, Han Lin, Jianlin Shi
Summary: A novel magneto-responsive nanocatalytic modality for cancer therapy, utilizing magnetostrictive-piezoelectric nanoparticles to catalyze the generation of cytotoxic reactive oxygen species in tumor cells, demonstrates promising prospects for future clinical translation due to its excellent catalytic dynamic responsiveness, high therapeutic efficacy, and biosafety in vivo.
Article
Engineering, Biomedical
Xiang Guo, Mintao Xue, Fei Chen, Qunfeng Guo, Xin Zhou, Han Lin, Yu Chen
Summary: In this study, a HA/MONs@miR-34a composite coating was successfully engineered to deliver miR-34a into osteoclasts and inhibit their differentiation and resorptive activity by regulating downstream gene expression in the early stage of fracture healing. The composite coating also showed potential for bone regeneration in a rat tibial fracture model. RNA-seq and functional enrichment analysis revealed the molecular mechanism underlying the regulation of osteoclast physiological functions by miR-34a.
Article
Chemistry, Multidisciplinary
Wencheng Wu, Yinying Pu, Heliang Yao, Han Lin, Jianlin Shi
Summary: This study reports a novel microbiotic nanomedicine LOD/TPZ@Lips-LA, which combines lactate oxidase (LOD) and prodrug tirapazamine (TPZ) loaded liposomes anchored on lactobacillus (LA) surface for chemotherapy-synergized antitumor immunity. The nanomedicine induces tumor cell apoptosis through in situ ROS generation and TPZ activation based on LA metabolism, resulting in strong immunogenic cell death (ICD). By regulating biomarker expression, it promotes anti-tumorigenic M1 polarization of innate tumor-associated macrophages and infiltration of adaptive cytotoxic CD8+ T cells, synergistically enhancing the tumor inhibition effects.
Article
Chemistry, Multidisciplinary
Han Lin, Chuang Yang, Yao Luo, Min Ge, Hao Shen, Xianlong Zhang, Jianlin Shi
Summary: In this study, we developed a biomimetic nanomedicine called HMMP for in situ vaccination against bacterial infection, focusing on osteomyelitis. The HMMP showed a burst release of bacterial antigens, triggering both cellular and humoral adaptive immune responses. Furthermore, it generated robust systemic antibacterial immunity and long-lasting bacteria-specific immune memory responses.
Article
Engineering, Environmental
Yuanhe Sun, Yuanxin Zhao, Zhaofeng Liang, Han Lin, Zhiguo Ren, Zeying Yao, Yaru Yin, Ping Huai, Ke Yang, Jinyou Lin, Yaobo Huang, Wen Wen, Xiaolong Li, Renzhong Tai, Daming Zhu
Summary: This study demonstrates the potential of silicene as an anode material for potassium-ion batteries. Through synthesis and testing, it is shown that silicene possesses efficient potassium-ion insertion/extraction properties and cycle stability.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Yanling You, Ya-Xuan Zhu, Junjie Jiang, Min Wang, Zhixin Chen, Chenyao Wu, Jie Wang, Wujie Qiu, Deliang Xu, Han Lin, Jianlin Shi
Summary: In this study, hydrogen-terminated silicon nanosheets (H-silicene) were synthesized through a simple wet-chemical exfoliation method. These nanosheets can react with environmental water to rapidly and continuously generate H-2 gas, without the need for external energy input. The study also revealed the mechanism behind the enhanced H-2 generation efficacy of H-silicene nanosheets. The synthesized H-silicene was demonstrated as a flexible hydrogen gas generator, with potential applications in acute inflammatory treatments by scavenging reactive oxygen species through on-demand H-2 production.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Nanoscience & Nanotechnology
Bo Yuan, Xin Zhou, Yingke Li, Yin Zhao, Mintao Xue, Qunfeng Guo, Gang Zheng, Xiongsheng Chen, Han Lin, Xiang Guo
Summary: This study demonstrates the excellent performance of a BPs@HA composite coating in eliminating bacterial biofilms and accelerating bone fracture healing. Through in vitro and in vivo studies on bacterial biofilms and bone fracture healing, the BPs@HA coating was shown to regulate the expression of core markers related to bone mesenchymal stem cell differentiation and bone formation through metabolism-related pathways.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Multidisciplinary Sciences
Chuang Yang, Yao Luo, Hao Shen, Min Ge, Jin Tang, Qiaojie Wang, Han Lin, Jianlin Shi, Xianlong Zhang
Summary: By modulating antigen presentation and co-stimulatory molecule expression, the use of PVP-modified MPS nanosheets can trigger a strong immune response against bacterial biofilm infections, reducing residual and recurrent infections.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Yanling You, Ya-Xuan Zhu, Junjie Jiang, Zhixin Chen, Chenyao Wu, Zhimin Zhang, Han Lin, Jianlin Shi
Summary: In this study, a new two-dimensional iodine nanomaterial called iodinene was proposed for the treatment of infectious diseases in vivo. Iodinene demonstrated enhanced antibacterial efficacy against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa by in situ production of active HIO and I2 molecules via allotropic transformation. The in vivo results further confirmed the desirable antibacterial efficacy of iodinene in treating bacterial wound infection and pneumonia.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Ya-Xuan Zhu, Yanling You, Zhixin Chen, Deliang Xu, Wenwen Yue, Xinxin Ma, Junjie Jiang, Wencheng Wu, Han Lin, Jianlin Shi
Summary: In this study, a copolymer-modified two-dimensional H-silicene nanomaterial was developed to help probiotics adapt to different gastrointestinal microenvironments. The material can resist acidic destruction in the stomach and generate anti-inflammatory hydrogen gas in the intestine, thus alleviating colitis. This strategy holds great significance for the development of intelligent self-adaptive materials.