4.7 Article

Sweet-flavored peptides with biological activities from mulberry seed protein treated by multifrequency countercurrent ultrasonic technology

期刊

FOOD CHEMISTRY
卷 367, 期 -, 页码 -

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ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.130647

关键词

Mulberry seed meal protein; Multifrequency countercurrent ultrasonic; treatment; Molecular diffusion dynamics model; Sweet-flavored peptide; Biological activity

资金

  1. Key Research and Development Program (Modern Agriculture) of Jiangsu Province [BE2019358]
  2. Jiangsu Agricultural Science and Technology Inno-vation Fund [CX (20) 2029]

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The study significantly increased the extraction yield of defatted mulberry seed protein (DMSP) using multifrequency countercurrent ultrasonic treatment, and obtained sweet-flavored peptides for the first time. A molecular diffusion dynamics model was developed to predict pretreatment conditions.
To increase the reuse of food residues, multifrequency countercurrent ultrasonic treatment was used to enhance the extraction yield of defatted mulberry seed protein (DMSP), and sweet-flavored peptides from DMSP hydrolysates (DMSPHs) were obtained for the first time. Here, the DMSP yield was increased by 16.2% (p < 0.05) while the power density was halved compared with single-frequency ultrasonic treatment. According to Fick's second law, a molecular diffusion dynamics model was developed to be suitable for predicting the pretreatment conditions (R2 = 0.9785). After that, the sweet-flavored peptides were purified and the main amino acid sequences were identified, i.e., FEGGSIE, KDFPEAHSQAT, and GSQPAEGAK. Moreover, the antioxidant activities of DMSPHs prepared with tri-frequency treatment was higher than 60%. The DMSPHs retarded the growth of HepG2 cells in vitro, increased the necrotic quadrant (Q1-UL), and extended the S phase. Therefore, the sweetflavored peptides prepared from DMSPHs using the multifrequency-ultrasonic treatment have significant biological activities.

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