Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate

Primary Cilia Formation Does Not Rely on WNT/β-Catenin Signaling

(2021) Ondrej Bernatik et al.   Frontiers in Cell and Developmental Biology

Patched 1 reduces the accessibility of cholesterol in the outer leaflet of membranes

(2021) Maia Kinnebrew et al.   eLife

Sterols in an intramolecular channel of Smoothened mediate Hedgehog signaling

(2020) Xiaofeng Qi et al.   Nature Chemical Biology

Cryo-EM structure of oxysterol-bound human Smoothened coupled to a heterotrimeric Gi

(2019) Xiaofeng Qi et al.   NATURE

Smoothened stimulation by membrane sterols drives Hedgehog pathway activity

(2019) Ishan Deshpande et al.   NATURE

Cholesterol accessibility at the ciliary membrane controls hedgehog signaling

(2019) Maia Kinnebrew et al.   eLife

The interplay of Patched, Smoothened and cholesterol in Hedgehog signaling

(2019) Ao Hu et al.   CURRENT OPINION IN CELL BIOLOGY

Mechanisms and regulation of cholesterol homeostasis

(2019) Jie Luo et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling

(2018) Dana Klatt Shaw et al.   DEVELOPMENTAL CELL

Structural basis for the recognition of Sonic Hedgehog by human Patched1

(2018) Xin Gong et al.   SCIENCE

Structures of human Patched and its complex with native palmitoylated sonic hedgehog

(2018) Xiaofeng Qi et al.   NATURE

Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex

(2018) Xiaofeng Qi et al.   SCIENCE

Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched

(2018) Yunxiao Zhang et al.   CELL

FlashPack: Fast and simple preparation of ultra-high performance capillary columns for LC-MS

(2018) Sergey I. Kovalchuk et al.   MOLECULAR & CELLULAR PROTEOMICS

Cholesterol Modification of Smoothened Is Required for Hedgehog Signaling

(2017) Xu Xiao et al.   MOLECULAR CELL

Rapid, direct activity assays for Smoothened reveal Hedgehog pathway regulation by membrane cholesterol and extracellular sodium

(2017) Benjamin R. Myers et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cellular Cholesterol Directly Activates Smoothened in Hedgehog Signaling

(2016) Pengxiang Huang et al.   CELL

Structural basis of Smoothened regulation by its extracellular domains

(2016) Eamon F. X. Byrne et al.   NATURE

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Cilia have high cAMP levels that are inhibited by Sonic Hedgehog-regulated calcium dynamics

(2016) Bryn S. Moore et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling

(2016) Giovanni Luchetti et al.   eLife

Fluorescent biosensors illuminate calcium levels within defined beta-cell endosome subpopulations

(2015) Tobias Albrecht et al.   CELL CALCIUM

High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells

(2014) Yuexin Zhou et al.   NATURE

Arborization of Dendrites by Developing Neocortical Neurons Is Dependent on Primary Cilia and Type 3 Adenylyl Cyclase

(2013) S. M. Guadiana et al.   JOURNAL OF NEUROSCIENCE

Scube/You activity mediates release of dually lipid-modified Hedgehog signal in soluble form

(2012) A. Creanga et al.   GENES & DEVELOPMENT

Oxysterols are allosteric activators of the oncoprotein Smoothened

(2012) Sigrid Nachtergaele et al.   Nature Chemical Biology

Dispatched and Scube Mediate the Efficient Secretion of the Cholesterol-Modified Hedgehog Ligand

(2012) Hanna Tukachinsky et al.   Cell Reports

Hedgehog-Regulated Ubiquitination Controls Smoothened Trafficking and Cell Surface Expression in Drosophila

(2012) Shuang Li et al.   PLOS BIOLOGY

Calcium Signaling in Live Cells on Elastic Gels under Mechanical Vibration at Subcellular Levels

(2011) Wagner Shin Nishitani et al.   PLoS One

Sonic hedgehog signaling is decoded by calcium spike activity in the developing spinal cord

(2011) Y. H. Belgacem et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Store-operated CRAC channels: function in health and disease

(2010) Anant B. Parekh   NATURE REVIEWS DRUG DISCOVERY

Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium

(2009) Ljiljana Milenkovic et al.   JOURNAL OF CELL BIOLOGY

G protein Gαi functions immediately downstream of Smoothened in Hedgehog signalling

(2008) Stacey K. Ogden et al.   NATURE

Oligomerization of STIM1 couples ER calcium depletion to CRAC channel activation

(2008) Riina M. Luik et al.   NATURE