Article
Chemistry, Analytical
Zahra Khoshbin, Elham Sameiyan, Hamed Zahraee, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: In this study, a simple aptasensing approach was used to detect ochratoxin A (OTA), a dangerous mycotoxin with severe health effects. The aptasensor utilized the alteration of liquid crystal (LC) molecules at the surfactant-arranged interface to sensitively detect OTA. By perturbing the alignment of LCs, the presence of OTA induced a noticeable change in the polarized view of the aptasensor substrate. The aptasensor demonstrated high sensitivity and could detect OTA in various real samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Applied
Zahra Khoshbin, Khalil Abnous, Seyed Mohammad Taghdisi, Asma Verdian, Elham Sameiyan, Mohammad Ramezani, Mona Alibolandi
Summary: A label-free aptasensor was developed for ultrasensitive quantification of OTA, with the ability to detect extremely low concentrations and monitor its presence in coffee and grape drink samples. This sensor provides promising insight for the manufacturing of real-time, cost-effective, and portable sensing devices for food control usage.
Article
Biophysics
Asma Verdian, Zahra Khoshbin, Chih-Hsin Chen
Summary: This article describes a liquid crystal-based aptasensor that detects OTA by changing the P-shaped DNA structure to trigger liquid crystal rearrangement. The developed sensor has a wide linear range, ultra-low detection limit, and high quantitative recovery, making it suitable for rapid, sensitive, and accurate detection of hazardous analytes in real samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Chemistry, Analytical
Zahra Khoshbin, Khalil Abnous, Seyed Mohammad Taghdisi, Asma Verdian
Summary: Advancements in liquid crystal technology have led to impressive developments in sensing platforms, enabling high sensitivity and excellent selectivity in biosensors for a wide range of applications. Liquid crystal-based biosensors show promise for label-free, cost-effective, and real-time detection, with the potential to be used on portable smartphone devices for on-site monitoring.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Optics
Mingce Chen, Zhexun Li, Taige Liu, Zhe Wang, Zhaomin Chen, Kewei Liu, Chai Hu, Mao Ye, Jiashuo Shi, Xinyu Zhang
Summary: Based on the electrically controlled birefringence effect in liquid crystal materials, a method for spatially separating azimuthally and radially polarized beams from non-polarized incident light waves is proposed. This method provides advantages such as low cost, miniaturization, and easy fabrication and integration, and can be applied over a wide wavelength range.
Article
Chemistry, Analytical
Zahra Khoshbin, Hamed Zahraee, Javad Zamanian, Asma Verdian, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: We developed an aptasensing array based on liquid crystal for monitoring tobramycin antibiotic. The direction of liquid crystal molecules changed from vertical to random due to the specific aptamer's conformational changes, indicating the presence of tobramycin. The aptasensor has the advantages of a wide linear concentration range, low limit of detection, and successful determination of tobramycin in milk and chicken egg samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Environmental Sciences
Zahra Zareshahrabadi, Mahdieh Karimirad, Keyvan Pakshir, Robab Bahmyari, Marjan Motamedi, Hasti Nouraei, Kamiar Zomorodian
Summary: Spices are essential in the diets of millions worldwide, but can be contaminated by fungi and mycotoxins. This study analyzed AFTs and OTA contamination in 80 spice samples, with the majority showing contamination, especially in curry samples. Only a small percentage of samples exceeded acceptable limits for AFTs and OTA.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Qi Guo, Mingjiang Zhang, Shanshan Zhao, Yajie Zhou, Yaxin Wang, Shan Jin, Jie Zhang, Hong-Bin Yao, Manzhou Zhu, Taotao Zhuang
Summary: In order to combat the increasing global counterfeiting, it is necessary to develop advanced anti-counterfeiting tags with unusual optical characters and complex security features, which has not yet been achieved. This study designs a stable circularly polarized luminescence (CPL) material that achieves multimodal-responsive, interactive/changeable information encryption-decryption, resembling a proof-of-principle blockchain-like integrated anticounterfeiting system. The obtained security materials are further encapsulated into a fiber to expand their functionality to flexible fabrics, creating an intelligent textile with color-adaptable function along with environmental change.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Materials Science, Multidisciplinary
Yong Li, Yanjun Liu, Dan Luo
Summary: This study demonstrates a photo-responsive liquid crystal elastomer (LCE) actuator driven by circularly polarized light. The actuator is composed of a cholesteric liquid crystal (CLC) film capable of reflecting and transmitting circularly polarized light, and a photothermal agent doped LCE film in a bilayer structure. An electrically controlled optical system with fast switching ability of circularly polarized light's handedness was developed to investigate the bending property of the proposed actuator under different periodic voltage schemes.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Xiao-Kang Liang, Qiang Zhu, Min Luo, Da-Quan Li
Summary: This paper employs optical microscopy and polarized light microscopy to characterize the microstructure of semisolid slurry and compares their reliability and accuracy. The effects of pouring temperature and mass of molten liquid on particle size and morphology are investigated. Results indicate significant influences of both factors on grain size and morphology.
Article
Materials Science, Multidisciplinary
Hamed Safaie, Mark Coleman, Richard Johnston, Amit Das, James Russell, Cameron Pleydell-Pearce
Summary: The link between microstructure, crystallographic texture, and mechanical properties is crucial for understanding the behavior of aluminum alloys. Electron backscatter diffraction (EBSD) has been widely used to determine these relationships but its cost and time-intensive nature limit its applicability in industrial quality assurance. This study explores the suitability of reflected circular polarized light microscopy (CPLM) as a rapid low-cost alternative to EBSD for cubic systems. The results demonstrate that CPLM can provide quantitative texture information for aluminum and verify orientation relationships between the substrate and surface oxide.
MATERIALS CHARACTERIZATION
(2022)
Article
Medical Laboratory Technology
Keyvan Pakshir, Andishe Dehghani, Hasti Nouraei, Zahra Zareshahrabadi, Kamiar Zomorodian
Summary: This study examined the presence of ochratoxin A and fungal contamination in coffee samples, finding that most samples were contaminated with ochratoxin A but only half had toxin levels exceeding safety thresholds. Predominant fungi isolated were Aspergillus species, highlighting the importance of carefully monitoring mycotoxins in coffee samples for quality improvement in the future.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Article
Materials Science, Multidisciplinary
Rodney J. McCabe, Daniel J. Savage, Abhishek Bhattacharyya, Renuka Gogusetti, David Alexander
Summary: Grain size and shape are important microstructure attributes that affect material properties. In this study, automated tools were developed to segment multi-angle polarized light microscopy (PLM) images for accurate measurement of grain size and shape statistics. The segmentation technique showed high accuracy in defining grain boundaries and twin features, making it a valuable tool for material analysis.
MATERIALS CHARACTERIZATION
(2022)
Article
Chemistry, Analytical
Qianjin Li, Baowen Liang, Wei Li, Weiwei Li, Jialong Sun, Saisai Jiao, Siwei Wang, Yu Jin, Tiesong Zheng, Jianlin Li
Summary: A portable analytical device based on SPCMs was developed for rapid and accurate detection of OTA, featuring automatic target extraction, point-of-care detection, and repeated testing capabilities.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Food Science & Technology
Maozhen Qu, Shijie Tian, Hengjie Yu, Da Liu, Chao Zhang, Yingchao He, Fang Cheng
Summary: Deoxynivalenol and zearalenone contamination in maize poses a threat to food safety. This study developed a cost-effective and non-destructive method for classifying contaminated maize. Texture and color features were extracted using visible light imaging and polarized light imaging, and six classification models were established. The results showed that the optimal detection surface was the endosperm side, and the SVM model achieved a classification accuracy of 88.1%. Further experiments verified the effectiveness of this method in identifying different contamination levels and reducing testing costs.
Article
Chemistry, Analytical
Elham Sameiyan, Parirokh Lavaee, Mohammad Ramezani, Mona Alibolandi, Zahra Khoshbin, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: A simple electrochemical sensing platform has been developed for the detection of aflatoxin B-1. This method showed enhanced sensitivity and selectivity, and can be applied to measure various toxins by substituting the proper aptamers and complementary strands.
Article
Chemistry, Analytical
Sedighe Khaksari, Amir Reza Ameri, Seyed Mohammad Taghdisi, Mahya Sabet, Seyed Mohammad Javad Ghaani Bami, Khalil Abnous, Seyed Ali Mousavi Shaegh
Summary: This paper presents a novel microfluidic electrochemical aptasensor for the determination of A549 cells. The sensor utilizes a target-induced structure switching mode to selectively and sensitively detect A549 cells. It has a wide linear dynamic range and a low detection limit, making it suitable for analysis in complex matrices.
Article
Pharmacology & Pharmacy
Tahoora Ghasemzadeh, Maliheh Hasannia, Khalil Abnous, Seyed Mohammad Taghdisi, Sirous Nekooei, Negar Nekooei, Mohammad Ramezani, Mona Alibolandi
Summary: The study designed and fabricated targeted nanobubbles with high encapsulation efficiency and sustained drug release. The decorated nanobubbles showed enhanced cellular uptake and cytotoxicity in MUC1-overexpressing cells. In vivo experiments demonstrated superior therapeutic efficacy and survival rate of the modified nanobubbles, and the echogenic capability was demonstrated through ultrasonic imaging.
EXPERT OPINION ON DRUG DELIVERY
(2023)
Article
Chemistry, Applied
Zahra Khoshbin, Marzieh Moeenfard, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: A simple aptasensor embedded in a micropipette tip is developed for label-free monitoring of acrylamide. The aptasensor utilizes the formation of a triple-helix molecular switch structure to induce the aggregation of gold nanoparticles. A smartphone imaging readout strategy enables the quantification of acrylamide based on the color change of the solution inside the micropipette tip. This aptasensor shows good selectivity and can successfully quantify acrylamide in various food samples.
Review
Chemistry, Multidisciplinary
Masoud Nejabat, Ali Samie, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: Gold nanorods (GNRs/AuNRs) are versatile gold nanoparticles that can be easily modified for biocompatible and functional nano-agents in cancer therapy. Their specific properties, such as absorption in the near-infrared (NIR) region, make them suitable for thermal ablation of cancer cells and induction of various therapeutic approaches. This review comprehensively summarizes the anticancer therapeutic capacities of AuNRs and their roles in the fabrication of different nano-constructs.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Spectroscopy
Hamed Zahraee, Zahra Khoshbin, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: In this study, a sensitive fluorescent method was developed using a hybrid structure of three aptamer strands and SYBR Green I (SGI) fluorescent dye for the detection of tobramycin (TOB) drug. The collapse of the hybridized aptamer skeleton to single strands due to preferential binding of aptamers to TOB resulted in the reduction of SGI molecules and quenching of the fluorescence response. The developed aptasensor showed superior target specificity with a detection limit of 0.153 pM and a wide linear dynamic range.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Sedighe Khaksari, Khalil Abnous, Farzin Hadizadeh, Mohammad Ramezani, Seyed Mohammad Taghdisi, Seyed Ali Mousavi Shaegh
Summary: Extracellular vesicles (EVs) are membrane-enclosed vesicles secreted from mammalian cells, which act as multi-component delivery vehicles for intercellular communications. Probing circulating EVs has great potential for early diagnostics of diseases, and biosensors have attracted attention in the identification of EVs due to their simplicity and portability. Amplification strategies, including various detection modalities and microfluidic systems, are essential for detecting the extremely low concentrations of EVs in bodily fluids at early stages of a disease.
Article
Chemistry, Analytical
Zahra Khoshbin, Elham Sameiyan, Hamed Zahraee, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: In this study, a simple aptasensing approach was used to detect ochratoxin A (OTA), a dangerous mycotoxin with severe health effects. The aptasensor utilized the alteration of liquid crystal (LC) molecules at the surfactant-arranged interface to sensitively detect OTA. By perturbing the alignment of LCs, the presence of OTA induced a noticeable change in the polarized view of the aptasensor substrate. The aptasensor demonstrated high sensitivity and could detect OTA in various real samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Ghasem Anbiaee, Zahra Khoshbin, Javad Zamanian, Ali Samie, Mohammad Ramezani, Mona Alibolandi, Noor Mohammd Danesh, Seyed Mohammad Taghdisi, Khalil Abnous
Summary: To rapidly and easily determine the detrimental effects of drugs such as cocaine on the human body, the development of sensing methods is highly necessary. We designed a cost-effective fluorescent aptasensor for cocaine detection using the signal enhancement capability of the UiO-66/AuNPs nanocomposite. The aptasensor achieved a linear response range of 0.5 & mu;M-20 & mu;M with a low detection limit of 0.178 & mu;M. This method has substantial benefits for the development of a low-cost and facile tool in medicine and forensic science.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Chemistry, Analytical
Masoomeh Esmaelpourfarkhani, Noor Mohammad Danesh, Mohammad Ramezani, Mona Alibolandi, Ali Khakshour Abdolabadi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: To combat drug trafficking and abuse, it is necessary to develop a sensing method for cocaine detection. This study proposes a label-free and simple split aptamer-based fluorescent aptasensor, which utilizes the N-methyl mesoporphyrin IX (NMM)/G-quadruplex system as a fluorescent indicator for cocaine detection. The working principle of the aptasensor is based on the formation of a split G-quadruplex structure in the presence of cocaine, leading to an increase in the emission intensity of fluorescence. Under optimized conditions, the designed fluorescent aptasensor exhibits a useful linear concentration range (50 pM to 25 nM) and a limit of detection (16 pM). The potential application of the designed aptasensor is demonstrated by successfully detecting cocaine in spiked human serum samples, with satisfying recoveries.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Ghasem Anbiaee, Zahra Khoshbin, Hamed Zahraee, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: Developing cost-effective smart sensing tools with ultrahigh sensitivity for food safety insurance is highly important. Aptamer-based biosensors powered by recycling signal amplification strategies, such as target recycling supported by enzymes, have shown great potential in achieving signal amplification. Exonucleases, as superior biocatalysts, simplify the amplification process and exhibit outstanding selectivity and sensitivity, making them promising sensing tools for label-free, ease-of-use, cost-effective, and real-time diagnosis of diverse targets. This article summarizes the achievements and perspectives in aptasensor design for qualitative monitoring of diverse targets using exonucleases for signal amplification.
Review
Chemistry, Analytical
Hamed Zahraee, Zahra Khoshbin, Fatemeh Mohammadi, Mansour Mashreghi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: Nucleic acids can form highly regular and complex structures at the nanoscale through self-assembly and programmability. The smallest type of junction, known as three-way junction (TWJ), can be formed by hybridizing three DNA or RNA strands. TWJ nanostructures provide multifunctional sites for biomolecular nanoarchitectures. This study discusses the achievements and future potential of TWJ-based biosensors, specifically aptasensors, for monitoring different targets.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Monireh Bolourinezhad, Majid Rezayi, Zahra Meshkat, Saman Soleimanpour, Majid Mojarrad, Farkhonde Zibadi, Seyed Hamid Aghaee-Bakhtiari, Seyed Mohammad Taghdisi
Summary: This study developed a rapid and accurate biosensor for detecting SARS-CoV-2. It utilized MXene nanosheets and carbon platinum to amplify the signal upon hybridization reaction. The biosensor showed a limit of detection of 0.4 aM and demonstrated high accuracy and sensitivity in clinical samples.
Review
Materials Science, Biomaterials
Ali Aliabadi, Maliheh Hasannia, Masoume Vakili-Azghandi, Fatemeh Araste, Khalil Abnous, Seyed Mohammad Taghdisi, Mohammad Ramezani, Mona Alibolandi
Summary: The formation of polymeric micelles via self-assembly is a versatile approach for improving the solubility and permeability of hydrophobic drugs. Polymer-based micelles have advantages in terms of size, morphology, and scalability. Various techniques have been employed to synthesize copolymers that can self-assemble into well-defined micellar structures with tunable drug release patterns. This review summarizes important polymerization techniques and recent achievements in the field of drug delivery using micellar systems, and proposes new visions for designing and synthesizing potent amphiphilic polymers.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Materials Science, Biomaterials
Ali Aliabadi, Maliheh Hasannia, Masoume Vakili-Azghandi, Fatemeh Araste, Khalil Abnous, Seyed Mohammad Taghdisi, Mohammad Ramezani, Mona Alibolandi
Summary: Polymeric micelles formed through self-assembly of amphiphilic polymers in aqueous environments have been explored as a versatile platform for enhancing the solubility and permeability of hydrophobic drugs. Compared to other self-assembly-based vehicles, polymeric micelles commonly have smaller size, spherical morphology, and simpler scale-up process. The synthesis of various amphiphilic polymers using different polymerization techniques has led to the development of smart copolymers that can form micelles, load therapeutics, and respond to external stimuli. This review summarizes important polymerization techniques and recent achievements in the field of drug delivery using micellar systems, and proposes new visions for the design and synthesis of innovative amphiphilic polymers for drug delivery applications. ROP, ATRP, RAFT, and combined methods were employed for copolymer synthesis, resulting in copolymers with proficiency of self-assembly into micellar structures.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)