4.7 Article

An ultra-sensitive dual-responsive aptasensor with combination of liquid crystal and intercalating dye molecules: A food toxin case study

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FOOD CHEMISTRY
卷 381, 期 -, 页码 -

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ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.132265

关键词

Aptasensor; Liquid crystal; Ochratoxin A; Mycotoxin; Fluorescence; Polarized light microscopy

资金

  1. National Institute for Medical Research Development (NIMAD) [996505]

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A label-free aptasensor was developed for ultrasensitive quantification of OTA, with the ability to detect extremely low concentrations and monitor its presence in coffee and grape drink samples. This sensor provides promising insight for the manufacturing of real-time, cost-effective, and portable sensing devices for food control usage.
Herein, a label-free aptasensor was designed through forming a double-stranded DNA skeleton on the glass substrate for ultrasensitive quantification of ochratoxin A (OTA) as a case study. The function fundament of the dual-responsive aptasensor was the perturbation of the vertical alignment of the liquid crystals (LCs) and intercalation of the SYBR Green I (SGI) dye molecules between the base pairs of the double-stranded DNA structure. The presence of OTA decomposed the double-stranded structure of DNA by releasing the OTA-specific aptamer from the sensing platform that induced an apparent alteration of the optical and fluorescent responses. The aptasensor specifically detected the ultra-low levels of OTA as 47.0E-9 pM (0.047 aM) and 34.0E-3 pM (34 fM) based on the polarized and fluorescent responses, respectively. The aptasensor monitored OTA in the coffee and grape drink samples. The aptasensor provides promising insight for manufacturing real-time, cost-effective, and portable sensing devices for food control usage.

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