Cryo-electron tomography provides topological insights into mutant huntingtin exon 1 and polyQ aggregates

TBK1 phosphorylates mutant Huntingtin and suppresses its aggregation and toxicity in Huntington's disease models

(2020) Ramanath Narayana Hegde et al.   EMBO JOURNAL

Protofilament Structure and Supramolecular Polymorphism of Aggregated Mutant Huntingtin Exon 1

(2020) Jennifer C. Boatz et al.   JOURNAL OF MOLECULAR BIOLOGY

Cryo-EM structures of tau filaments

(2020) Sjors HW Scheres et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Multi-scale 3D Cryo-Correlative Microscopy for Vitrified Cells

(2020) Gong-Her Wu et al.   STRUCTURE

Huntingtin Lowering Strategies for Disease Modification in Huntington’s Disease

(2019) Sarah J. Tabrizi et al.   NEURON

Advances in cryo-electron tomography and subtomogram averaging and classification

(2019) Peijun Zhang   CURRENT OPINION IN STRUCTURAL BIOLOGY

Cryo-EM of amyloid fibrils and cellular aggregates

(2019) Anthony WP Fitzpatrick et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

A complete data processing workflow for cryo-ET and subtomogram averaging

(2019) Muyuan Chen et al.   NATURE METHODS

Self-assembly of Mutant Huntingtin Exon-1 Fragments into Large Complex Fibrillar Structures Involves Nucleated Branching

(2018) Anne S. Wagner et al.   JOURNAL OF MOLECULAR BIOLOGY

Molecular and structural architecture of polyQ aggregates in yeast

(2018) Anselm Gruber et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cryo-EM structure of alpha-synuclein fibrils

(2018) Ricardo Guerrero-Ferreira et al.   eLife

Neutralizing Antibodies Inhibit Chikungunya Virus Budding at the Plasma Membrane

(2018) Jing Jin et al.   Cell Host & Microbe

Amyloid fibril structure of α-synuclein determined by cryo-electron microscopy

(2018) Yaowang Li et al.   CELL RESEARCH

Huntington disease: A quarter century of progress since the gene discovery

(2018) Claudia M. Testa et al.   JOURNAL OF THE NEUROLOGICAL SCIENCES

Reducing effects of particle adsorption to the air–water interface in cryo-EM

(2018) Alex J. Noble et al.   NATURE METHODS

A new era for understanding amyloid structures and disease

(2018) Matthew G. Iadanza et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Cryo-EM of full-length α-synuclein reveals fibril polymorphs with a common structural kernel

(2018) Binsen Li et al.   Nature Communications

Monomeric Huntingtin Exon 1 Has Similar Overall Structural Features for Wild-Type and Pathological Polyglutamine Lengths

(2017) John B. Warner et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Therapies targeting DNA and RNA in Huntington's disease

(2017) Edward J Wild et al.   LANCET NEUROLOGY

Polyglutamine inclusion body toxicity

(2017) Roger L. Albin   MOVEMENT DISORDERS

Cryo-EM structures of tau filaments from Alzheimer’s disease

(2017) Anthony W. P. Fitzpatrick et al.   NATURE

Convolutional neural networks for automated annotation of cellular cryo-electron tomograms

(2017) Muyuan Chen et al.   NATURE METHODS

Fibril structure of amyloid-β(1–42) by cryo–electron microscopy

(2017) Lothar Gremer et al.   SCIENCE

Fibril polymorphism affects immobilized non-amyloid flanking domains of huntingtin exon1 rather than its polyglutamine core

(2017) Hsiang-Kai Lin et al.   Nature Communications

An Intein-based Strategy for the Production of Tag-free Huntingtin Exon 1 Proteins Enables New Insights into the Polyglutamine Dependence of Httex1 Aggregation and Fibril Formation

(2016) Sophie Vieweg et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

ICON: 3D reconstruction with ‘missing-information’ restoration in biological electron tomography

(2016) Yuchen Deng et al.   JOURNAL OF STRUCTURAL BIOLOGY

Alignment algorithms and per-particle CTF correction for single particle cryo-electron tomography

(2016) Jesús G. Galaz-Montoya et al.   JOURNAL OF STRUCTURAL BIOLOGY

Quantifying Variability of Manual Annotation in Cryo-Electron Tomograms

(2016) Corey W. Hecksel et al.   MICROSCOPY AND MICROANALYSIS

Huntingtin exon 1 fibrils feature an interdigitated β-hairpin–based polyglutamine core

(2016) Cody L. Hoop et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nanoscale studies link amyloid maturity with polyglutamine diseases onset

(2016) F. S. Ruggeri et al.   Scientific Reports

Control of the structural landscape and neuronal proteotoxicity of mutant Huntingtin by domains flanking the polyQ tract

(2016) Koning Shen et al.   eLife

Structural Mechanisms of Mutant Huntingtin Aggregation Suppression by the Synthetic Chaperonin-like CCT5 Complex Explained by Cryoelectron Tomography

(2015) Michele C. Darrow et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Single particle tomography in EMAN2

(2015) Jesús G. Galaz-Montoya et al.   JOURNAL OF STRUCTURAL BIOLOGY

Conformational switch of polyglutamine-expanded huntingtin into benign aggregates leads to neuroprotective effect

(2015) Chia-Sui Sun et al.   Scientific Reports

Super-Resolution Fluorescence of Huntingtin Reveals Growth of Globular Species into Short Fibers and Coexistence of Distinct Aggregates

(2014) Whitney C. Duim et al.   ACS Chemical Biology

Polyglutamine Amyloid Core Boundaries and Flanking Domain Dynamics in Huntingtin Fragment Fibrils Determined by Solid-State Nuclear Magnetic Resonance

(2014) Cody L. Hoop et al.   BIOCHEMISTRY

Polyglutamine (PolyQ) Diseases: Genetics to Treatments

(2014) Hueng-Chuen Fan et al.   CELL TRANSPLANTATION

M-free: Scoring the reference bias in sub-tomogram averaging and template matching

(2014) Zhou Yu et al.   JOURNAL OF STRUCTURAL BIOLOGY

The huntingtin N17 domain is a multifunctional CRM1 and Ran-dependent nuclear and cilial export signal

(2013) T. Maiuri et al.   HUMAN MOLECULAR GENETICS

Unmasking the roles of N- and C-terminal flanking sequences from exon 1 of huntingtin as modulators of polyglutamine aggregation

(2013) S. L. Crick et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

TRiC’s tricks inhibit huntingtin aggregation

(2013) Sarah H Shahmoradian et al.   eLife

Use of Fluorescent Probes: Their Effect on Cell Biology and Limitations

(2012) Ellen C. Jensen   Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology

Structural Features and Domain Organization of Huntingtin Fibrils

(2012) Charles W. Bugg et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Physical Chemistry of Polyglutamine: Intriguing Tales of a Monotonous Sequence

(2012) Ronald Wetzel   JOURNAL OF MOLECULAR BIOLOGY

Automated tracing of filaments in 3D electron tomography reconstructions using Sculptor and Situs

(2012) Mirabela Rusu et al.   JOURNAL OF STRUCTURAL BIOLOGY

IPET and FETR: Experimental Approach for Studying Molecular Structure Dynamics by Cryo-Electron Tomography of a Single-Molecule Structure

(2012) Lei Zhang et al.   PLoS One

Sub-Diffraction Imaging of Huntingtin Protein Aggregates by Fluorescence Blink-Microscopy and Atomic Force Microscopy

(2011) Whitney C. Duim et al.   CHEMPHYSCHEM

Structural Characterization of Polyglutamine Fibrils by Solid-State NMR Spectroscopy

(2011) Robert Schneider et al.   JOURNAL OF MOLECULAR BIOLOGY

Automated segmentation of electron tomograms for a quantitative description of actin filament networks

(2011) Alexander Rigort et al.   JOURNAL OF STRUCTURAL BIOLOGY

The Aggregation-Enhancing Huntingtin N-Terminus Is Helical in Amyloid Fibrils

(2011) V. N. Sivanandam et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Identical oligomeric and fibrillar structures captured from the brains of R6/2 and knock-in mouse models of Huntington's disease

(2009) Kirupa Sathasivam et al.   HUMAN MOLECULAR GENETICS

Distinct conformations of in vitro and in vivo amyloids of huntingtin-exon1 show different cytotoxicity

(2009) Y. Nekooki-Machida et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Exploring the structure and function paradigm

(2008) Oliver C. Redfern et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Classification and 3D averaging with missing wedge correction in biological electron tomography☆

(2008) A. Bartesaghi et al.   JOURNAL OF STRUCTURAL BIOLOGY

Towards a transgenic model of Huntington’s disease in a non-human primate

(2008) Shang-Hsun Yang et al.   NATURE