Synthetic auxotrophy remains stable after continuous evolution and in coculture with mammalian cells

TrkA undergoes a tetramer-to-dimer conversion to open TrkH which enables changes in membrane potential

(2020) Hanzhi Zhang et al.   Nature Communications

Immunotherapy with engineered bacteria by targeting the STING pathway for anti-tumor immunity

(2020) Daniel S. Leventhal et al.   Nature Communications

Developing a new class of engineered live bacterial therapeutics to treat human diseases

(2020) Mark R. Charbonneau et al.   Nature Communications

Synthetic Biology Approaches to Engineer Probiotics and Members of the Human Microbiota for Biomedical Applications

(2018) Josef R. Bober et al.   Annual Review of Biomedical Engineering

Precise, automated control of conditions for high-throughput growth of yeast and bacteria with eVOLVER

(2018) Brandon G Wong et al.   NATURE BIOTECHNOLOGY

The social network of microorganisms — how auxotrophies shape complex communities

(2018) Karsten Zengler et al.   NATURE REVIEWS MICROBIOLOGY

Adaptive evolution of genomically recodedEscherichia coli

(2018) Timothy M. Wannier et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering posttranslational proofreading to discriminate nonstandard amino acids

(2018) Aditya M. Kunjapur et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Essential Genome of Escherichia coli K-12

(2018) Emily C. A. Goodall et al.   mBio

Multiple entry pathways within the efflux transporter AcrB contribute to multidrug recognition

(2018) Martijn Zwama et al.   Nature Communications

Resolving host–microbe interactions in the gut: the promise of in vitro models to complement in vivo research

(2018) Wilmes Paul et al.   CURRENT OPINION IN MICROBIOLOGY

Synthetic Biology and Engineered Live Biotherapeutics: Toward Increasing System Complexity

(2018) Tanel Ozdemir et al.   Cell Systems

Switch Loop Flexibility Affects Substrate Transport of the AcrB Efflux Pump

(2017) Reinke T. Müller et al.   JOURNAL OF MOLECULAR BIOLOGY

Design of live attenuated bacterial vaccines based on D-glutamate auxotrophy

(2017) Maria P. Cabral et al.   Nature Communications

A primary human macrophage-enteroid co-culture model to investigate mucosal gut physiology and host-pathogen interactions

(2017) Gaelle Noel et al.   Scientific Reports

Substrate transport pathway inside outward open conformation of EmrD: a molecular dynamics simulation study

(2016) Tan Xianwei et al.   Molecular BioSystems

A microfluidics-based in vitro model of the gastrointestinal human–microbe interface

(2016) Pranjul Shah et al.   Nature Communications

Protein Synthesis with Ribosomes Selected for the Incorporation of β-Amino Acids

(2015) Rumit Maini et al.   BIOCHEMISTRY

How informative is the mouse for human gut microbiota research?

(2015) T. L. A. Nguyen et al.   Disease Models & Mechanisms

Recoded organisms engineered to depend on synthetic amino acids

(2015) Alexis J. Rovner et al.   NATURE

Biocontainment of genetically modified organisms by synthetic protein design

(2015) Daniel J. Mandell et al.   NATURE

Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip

(2015) Hyun Jung Kim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Metabolic cross-feeding via intercellular nanotubes among bacteria

(2015) Samay Pande et al.   Nature Communications

Inexpensive Multiplexed Library Preparation for Megabase-Sized Genomes

(2015) Michael Baym et al.   PLoS One

Recent progress on the structure and function of the TrkH/KtrB ion channel

(2014) Elena J Levin et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Co-culture systems and technologies: taking synthetic biology to the next level

(2014) L. Goers et al.   Journal of the Royal Society Interface

Building a morbidostat: an automated continuous-culture device for studying bacterial drug resistance under dynamically sustained drug inhibition

(2013) Erdal Toprak et al.   Nature Protocols

Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow

(2012) Hyun Jung Kim et al.   LAB ON A CHIP

Identification and Analysis of the Putative Pentose Sugar Efflux Transporters in Escherichia coli

(2012) Khushnuma Koita et al.   PLoS One

Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop

(2012) T. Eicher et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Sequential Mechanism of Assembly of Multidrug Efflux Pump AcrAB-TolC

(2011) Elena B. Tikhonova et al.   CHEMISTRY & BIOLOGY

Structures of the multidrug exporter AcrB reveal a proximal multisite drug-binding pocket

(2011) Ryosuke Nakashima et al.   NATURE

Lysine Auxotrophy Combined with Deletion of the SecA2 Gene Results in a Safe and Highly Immunogenic Candidate Live Attenuated Vaccine for Tuberculosis

(2011) Joseph Hinchey et al.   PLoS One

Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

(2011) Farren J. Isaacs et al.   SCIENCE

Co-culture of epithelial cells and bacteria for investigating host–pathogen interactions

(2009) Jeongyun Kim et al.   LAB ON A CHIP

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE