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Biochemical Research Methods
Aseer Intisar, Seung Joon Lee, Yu-Gyeong Kim, Woon-Hae Kim, Hyun Young Shin, Min Young Kim, Jong Man Kim, Jungmin Lee, Yun Jeoung Mo, Yu Seon Kim, Seung-Hoon Kim, Yun-Il Lee, Minseok S. Kim
Summary: This study introduces a rapid method for enriching neurons from the mouse embryonic dorsal root ganglion using fully-automated continuous centrifugal microfluidics, achieving effective isolation within 13 minutes. The purified neurons showed improved viability and purity compared to conventional chemical purification methods, highlighting the potential of this automated system to advance neurological research.
Review
Pharmacology & Pharmacy
Andrei Sofia Barros, Ana Costa, Bruno Sarmento
Summary: Drug development is a critical step in the pharmaceutical industry pipeline, and traditional cell culture and animal models have gaps in predicting and translating human pharmacokinetic and pharmacodynamic parameters. Advances in tissue engineering have allowed the combination of cell biology with microengineering techniques, providing alternatives to traditional preclinical models. Organ-on-a-chips and 3D bioprinting models have the potential to simulate the physiological and pathological microenvironment of living organs and tissues, offering more realistic models for drug absorption, distribution, metabolism, and excretion.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Biochemical Research Methods
Ian C. Harding, Nicholas R. O'Hare, Mark Vigliotti, Alex Caraballo, Claire Lee, Karina Millican, Ira M. Herman, Eno E. Ebong
Summary: The function of blood-brain barrier endothelial cells is influenced by flow conditions and supportive cells, necessitating the use of physiologically relevant multicellular models to investigate and understand their role.
Article
Chemistry, Analytical
Xin Shi, Beibei Ma, Hongyu Chen, Wei Tan, Shiqing Ma, Guorui Zhu
Summary: This paper presents the development of a microfluidic/chitosan device for drug release to inhibit infections and guide bone regeneration. The device offers controlled drug release, flexible clinical use, low cost, and excellent antibacterial performance and biocompatibility.
Article
Chemistry, Analytical
Martin Ruzicka, Mihkel Kaljurand, Jelena Gorbatsova, Merike Vaher, Jekaterina Mazina-Sinkar
Summary: Currently used methods for in-field determination of illegal drugs often suffer from unreliable results. In this study, a fully automated sample pretreatment prototype was developed, which could determine illegal drugs in oral fluid and be used for confirmatory analysis. The developed extraction device was easy to use and suitable for field conditions.
Article
Chemistry, Multidisciplinary
Subbarao Yalamanchili, Tu-Anh Nguyen, Alexander Zsikla, Gavin Stamper, Ashley E. DeYong, John Florek, Olivea Vasquez, Nicola L. B. Pohl, Clay S. Bennett
Summary: An automated continuous flow system capable of producing protected deoxy-sugar donors from commercial material is described. The system achieved synthesis of multiple donors in parallel through modular design, with reactions able to be concatenated into a continuous process. The fully automated continuous flow processes were demonstrated using an open-source Python-controlled automation platform.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Multidisciplinary Sciences
Mohammad S. Imtiaz, Charles Bandoian, Thomas J. Santoro
Summary: Opioid use disorder is a worsening epidemic with over 100,000 deaths recorded in 2021. Current antidotes must be administered within a critical time window to be effective, leading to many deaths due to lack of timely intervention. A wearable, on-demand system has been designed to automatically detect, report, and treat opioid overdoses, potentially saving lives lost to this increasing epidemic.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, General & Internal
Pei-Chin Lin, Chia-Ling Chang, Yi-Hua Chung, Chih-Chun Chang, Fang-Yeh Chu
Summary: Blood volume and number of culture sets are important factors affecting blood culture positivity rate. The study finds that total blood culture volume and diagnosis of sepsis are critical factors influencing positivity rate. However, the proportion of blood culture bottles with optimal blood volume is low.
Review
Biotechnology & Applied Microbiology
Sandra Perez-Rodriguez, Jose Manuel Garcia-Aznar, Jesus Gonzalo-Asensio
Summary: Understanding bacterial responses in different microenvironments is crucial for studying diseases, biofilm formation, and antimicrobial resistance. Microfluidics technology allows precise control of test conditions at a micrometric scale, enabling the simulation of physiological 3D environments.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Medicine, Research & Experimental
Haofan Liu, Bingwen Zhang, Yanna Chen, Zhe Cui, Liandong Hu
Summary: The objective of this study was to explore an innovative sustained release technology and design a new microporous resin-based polymer device (RPD) for controlled release of glipizide (GZ). The results showed that the resin layer prepared by photocurable resin can effectively control the release of GZ and exhibit excellent in vitro release and in vivo pharmacokinetic properties.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemical Research Methods
David Ross, Peter D. Tonner, Olga B. Vasilyeva
Summary: In this study, the authors describe a method for reproducible microbial culture using laboratory automation systems and provide statistical analysis of data from 150 automated microbial growth experiments.
Article
Biochemical Research Methods
Ayomikun Esan, Frederique Vanholsbeeck, Simon Swift, Cushla M. M. McGoverin
Summary: With the global increase in food exchange, it has become crucial to efficiently separate bacterial cells from debris in order to protect consumers from bacterial contamination. In this study, passive-based inertial microfluidics was used to separate bacterial cells from debris extracted from ground meat and meat swabs, achieving average recovery efficiencies of approximately 80% and 70%, respectively.
Article
Chemistry, Multidisciplinary
Kehua Xu, Jingjing Zhang, Wenrui Ma, Hui Huang, Shiqiang Yan, Li Wang, Weijia Zhang
Summary: This study aimed to develop a simple device that could simulate different pressure environments and reveal the cellular responses to pressure stimulus. Biological experiments using human umbilical vein endothelial cells showed that high hydrostatic pressure led to more stereoscopic nuclear morphology and redistribution of lamin A/C. Proteomics analysis showed significant changes in mitochondria-related pathways, indicating alterations in mitochondrial behaviors. This device can be widely applied in biological research and enhance our understanding of cellular mechano-sensation.
CHINESE CHEMICAL LETTERS
(2023)
Article
Immunology
Yan Zhou, Shuping Zhou, Jun Peng, Liang Min, Qiang Chen, Jiangwei Ke
Summary: This study investigated the distribution and drug resistance of bacteria in clinical blood culture specimens from children in Jiangxi province. A total of 7,977 bacterial strains were isolated, with Gram-positive cocci being the most commonly isolated pathogens. The study highlights the importance of closely monitoring antimicrobial resistance and using antimicrobial agents with caution in the treatment of bloodstream infections in children.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Medicinal
Michael Flanagan, Qi Gan, Saahil Sheth, Rachel Schafer, Samuel Ruesing, Linda E. Winter, Karoly Toth, Silviya P. Zustiak, Adriana M. Montano
Summary: Morquio A disease is a genetic disorder caused by GALNS deficiency, and patients are treated with enzyme replacement therapy. This study explored PEG hydrogels as a drug delivery system for sustained release of rhGALNS. The optimized hydrogel formulation achieved sustained release of active rhGALNS for up to 28 days in vitro and up to 20 days in vivo, suggesting that PEG hydrogels could be a promising method for improving patient quality of life.
Article
Biochemistry & Molecular Biology
Allon M. Klein, Linas Mazutis, Ilke Akartuna, Naren Tallapragada, Adrian Veres, Victor Li, Leonid Peshkin, David A. Weitz, Marc W. Kirschner
Article
Multidisciplinary Sciences
Adam C. Palmer, Erdal Toprak, Michael Baym, Seungsoo Kim, Adrian Veres, Shimon Bershtein, Roy Kishony
NATURE COMMUNICATIONS
(2015)
Article
Biochemistry & Molecular Biology
Laura K. Stone, Michael Baym, Tami D. Lieberman, Remy Chait, Jon Clardy, Roy Kishony
NATURE CHEMICAL BIOLOGY
(2016)
Article
Biochemical Research Methods
Rapolas Zilionis, Juozas Nainys, Adrian Veres, Virginia Savova, David Zemmour, Allon M. Klein, Linas Mazutis
Article
Multidisciplinary Sciences
Michael Baym, Tami D. Lieberman, Eric D. Kelsic, Remy Chait, Rotem Gross, Idan Yelin, Roy Kishony
Article
Multidisciplinary Sciences
Remy Chait, Adam C. Palmer, Idan Yelin, Roy Kishony
NATURE COMMUNICATIONS
(2016)
Article
Biochemistry & Molecular Biology
Maayan Baron, Adrian Veres, Samuel L. Wolock, Aubrey L. Faust, Renaud Gaujoux, Amedeo Vetere, Jennifer Hyoje Ryu, Bridget K. Wagner, Shai S. Shen-Orr, Allon M. Klein, Douglas A. Melton, Itai Yanai
Article
Multidisciplinary Sciences
Remy Chait, Jakob Ruess, Tobias Bergmiller, Gasper Tkacik, Calin C. Guet
NATURE COMMUNICATIONS
(2017)
Article
Cell & Tissue Engineering
Adrian Veres, Bridget S. Gosis, Qiurong Ding, Ryan Collins, Ashok Ragavendran, Harrison Brand, Serkan Erdin, Chad A. Cowan, Michael E. Talkowski, Kiran Musunuru
Article
Multidisciplinary Sciences
Silvia J. Canas-Duarte, Silvia Restrepo, Juan Manuel Pedraza
Article
Multidisciplinary Sciences
Scott Dryden-Peterson, Kara Bennett, Michael D. Hughes, Adrian Veres, Oaitse John, Rosina Pradhananga, Matthew Boyer, Carolyn Brown, Bright Sakyi, Erik van Widenfelt, Koona Keapoletswe, Madisa Mine, Sikhulile Moyo, Aida Asmelash, Mark Siedner, Mompati Mmalane, Roger L. Shapiro, Shahin Lockman
Article
Multidisciplinary Sciences
Adrian Veres, Aubrey L. Faust, Henry L. Bushnell, Elise N. Engquist, Jennifer Hyoje-Ryu Kenty, George Harb, Yeh-Chuin Poh, Elad Sintov, Mads Gurtler, Felicia W. Pagliuca, Quinn P. Peterson, Douglas A. Melton
Article
Multidisciplinary Sciences
Quinn P. Peterson, Adrian Veres, Lihua Chen, Michael Q. Slama, Jennifer H. R. Kenty, Shaimaa Hassoun, Matthew R. Brown, Haiqiang Dou, Caden D. Duffy, Quan Zhou, Aleksey V. Matveyenko, Bjoern Tyrberg, Maria Soerhede-Winzell, Patrik Rorsman, Douglas A. Melton
NATURE COMMUNICATIONS
(2020)
Article
Chemistry, Analytical
David C. Duran, Cesar A. Hernandez, Elizabeth Suesca, Ruben Acevedo, Ivon M. Acosta, Diana A. Forero, Francisco E. Rozo, Juan M. Pedraza
Summary: Researchers have designed and fabricated a device that uses the slipstreaming effect to study the growth and aging of yeast cells under minimal mechanical pressure influence. This device is relatively inexpensive and easy to manufacture, helping to eliminate pressure bias in future studies.
Article
Biochemical Research Methods
Andela Davidovic, Remy Chait, Gregory Batt, Jakob Ruess
Summary: Researchers investigate different approaches for calculating parameter likelihoods of single-cell longitudinal data based on approximations of the chemical master equation (CME), with a focus on coupling linear noise approximation (LNA) or moment closure methods to a Kalman filter. They show that coupling LNA to a Kalman filter allows accurate approximation of likelihoods and inference of model parameters from data, even in cases where LNA provides poor approximations of CME. The computational cost of filtering-based iterative likelihood evaluation scales advantageously in terms of measurement times and different input perturbations, making it well-suited for data obtained from modern experimental platforms.
PLOS COMPUTATIONAL BIOLOGY
(2022)