Structural basis of substrate recognition and thermal protection by a small heat shock protein

Conditional Disorder in Small Heat-shock Proteins

(2020) T. Reid Alderson et al.   JOURNAL OF MOLECULAR BIOLOGY

Release of a disordered domain enhances HspB1 chaperone activity toward tau

(2020) Hannah E. R. Baughman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plant small heat shock proteins – evolutionary and functional diversity

(2020) Elizabeth R. Waters et al.   NEW PHYTOLOGIST

Dodecameric structure of a small Heat Shock Protein from Mycobacterium marinum M

(2019) Spraha Bhandari et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Local unfolding of the HSP27 monomer regulates chaperone activity

(2019) T. Reid Alderson et al.   Nature Communications

The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation–suppressing activity

(2019) Di Wu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

HspB1 phosphorylation regulates its intramolecular dynamics and mechanosensitive molecular chaperone interaction with filamin C

(2019) Miranda P. Collier et al.   Science Advances

X-ray crystallography–based structural elucidation of enzyme-bound intermediates along the 1‑deoxy‑d‑xylulose 5-phosphate synthase reaction coordinate

(2019) Percival Yang-Ting Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

cryo_fit: Democratization of Flexible Fitting for Cryo-EM

(2019) Doo Nam Kim et al.   JOURNAL OF STRUCTURAL BIOLOGY

Current Developments in Coot for Macromolecular Model Building of Electron Cryo‐microscopy and Crystallographic Data

(2019) Ana Casañal et al.   PROTEIN SCIENCE

The influence of the N-terminal region proximal to the core domain on the assembly and chaperone activity of αB-crystallin

(2018) Blagojce Jovcevski et al.   CELL STRESS & CHAPERONES

SWISS-MODEL: homology modelling of protein structures and complexes

(2018) Andrew Waterhouse et al.   NUCLEIC ACIDS RESEARCH

Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions

(2018) Georg K. A. Hochberg et al.   SCIENCE

It takes a dimer to tango: Oligomeric small heat shock proteins dissociate to capture substrate

(2018) Indu Santhanagopalan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Small heat shock proteins: Simplicity meets complexity

(2018) Martin Haslbeck et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

New tools for automated high-resolution cryo-EM structure determination in RELION-3

(2018) Jasenko Zivanov et al.   eLife

Structural model of dodecameric heat-shock protein Hsp21: Flexible N-terminal arms interact with client proteins while C-terminal tails maintain the dodecamer and chaperone activity

(2017) Gudrun Rutsdottir et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Addressing preferred specimen orientation in single-particle cryo-EM through tilting

(2017) Yong Zi Tan et al.   NATURE METHODS

Identification of core subunits of photosystem II as action sites of HSP21, which is activated by the GUN5-mediated retrograde pathway in Arabidopsis

(2017) Si-Ting Chen et al.   PLANT JOURNAL

Conformational dynamics of 1-deoxy-d-xylulose 5-phosphate synthase on ligand binding revealed by H/D exchange MS

(2017) Jieyu Zhou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

UCSF ChimeraX: Meeting modern challenges in visualization and analysis

(2017) Thomas D. Goddard et al.   PROTEIN SCIENCE

Chaperone-client interactions between Hsp21 and client proteins monitored in solution by small angle X-ray scattering and captured by crosslinking mass spectrometry

(2017) Gudrun Rutsdottir et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Structural Basis for the Interaction of a Human Small Heat Shock Protein with the 14-3-3 Universal Signaling Regulator

(2017) Nikolai N. Sluchanko et al.   STRUCTURE

Interference with plastome gene expression and Clp protease activity in Arabidopsis triggers a chloroplast unfolded protein response to restore protein homeostasis

(2017) Ernesto Llamas et al.   PLoS Genetics

Model-based local density sharpening of cryo-EM maps

(2017) Arjen J Jakobi et al.   eLife

Small Heat-shock Proteins Prevent α-Synuclein Aggregation via Transient Interactions and Their Efficacy Is Affected by the Rate of Aggregation

(2016) Dezerae Cox et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

The plastid metalloprotease FtsH6 and small heat shock protein HSP21 jointly regulate thermomemory in Arabidopsis

(2016) Mastoureh Sedaghatmehr et al.   Nature Communications

Small heat shock proteins sequester misfolding proteins in near-native conformation for cellular protection and efficient refolding

(2016) Sophia Ungelenk et al.   Nature Communications

Specific Hsp100 Chaperones Determine the Fate of the First Enzyme of the Plastidial Isoprenoid Pathway for Either Refolding or Degradation by the Stromal Clp Protease in Arabidopsis

(2016) Pablo Pulido et al.   PLoS Genetics

Differential Subplastidial Localization and Turnover of Enzymes Involved in Isoprenoid Biosynthesis in Chloroplasts

(2016) Catalina Perello et al.   PLoS One

Compartment-specific aggregases direct distinct nuclear and cytoplasmic aggregate deposition

(2015) S. B. Miller et al.   EMBO JOURNAL

The Phyre2 web portal for protein modeling, prediction and analysis

(2015) Lawrence A Kelley et al.   Nature Protocols

iMODFIT: Efficient and robust flexible fitting based on vibrational analysis in internal coordinates

(2013) José Ramón Lopéz-Blanco et al.   JOURNAL OF STRUCTURAL BIOLOGY

Spatial sequestration of misfolded proteins by a dynamic chaperone pathway enhances cellular fitness during stress

(2013) Stéphanie Escusa-Toret et al.   NATURE CELL BIOLOGY

Chloroplast Small Heat Shock Protein HSP21 Interacts with Plastid Nucleoid Protein pTAC5 and Is Essential for Chloroplast Development in Arabidopsis under Heat Stress

(2013) L. Zhong et al.   PLANT CELL

Arabidopsis J-Protein J20 Delivers the First Enzyme of the Plastidial Isoprenoid Pathway to Protein Quality Control

(2013) P. Pulido et al.   PLANT CELL

The evolution, function, structure, and expression of the plant sHSPs

(2012) Elizabeth R. Waters   JOURNAL OF EXPERIMENTAL BOTANY

Prevention of overfitting in cryo-EM structure determination

(2012) Sjors H W Scheres et al.   NATURE METHODS

NIH Image to ImageJ: 25 years of image analysis

(2012) Caroline A Schneider et al.   NATURE METHODS

Hsp42 is required for sequestration of protein aggregates into deposition sites inSaccharomyces cerevisiae

(2011) Sebastian Specht et al.   JOURNAL OF CELL BIOLOGY

Small heat shock proteins and α-crystallins: dynamic proteins with flexible functions

(2011) Eman Basha et al.   TRENDS IN BIOCHEMICAL SCIENCES

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Independent evolution of the core domain and its flanking sequences in small heat shock proteins

(2010) Thomas Kriehuber et al.   FASEB JOURNAL

Solid-state NMR and SAXS studies provide a structural basis for the activation of αB-crystallin oligomers

(2010) Stefan Jehle et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Quaternary dynamics and plasticity underlie small heat shock protein chaperone function

(2010) F. Stengel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

(2009) Roberto Fernandez-Lafuente   ENZYME AND MICROBIAL TECHNOLOGY

Crystal Structures of α-Crystallin Domain Dimers of αB-Crystallin and Hsp20

(2009) C. Bagnéris et al.   JOURNAL OF MOLECULAR BIOLOGY

Real-Time Monitoring of Protein Complexes Reveals their Quaternary Organization and Dynamics

(2008) Alexander J. Painter et al.   CHEMISTRY & BIOLOGY

Insights into Small Heat Shock Protein and Substrate Structure during Chaperone Action Derived from Hydrogen/Deuterium Exchange and Mass Spectrometry

(2008) Guilong Cheng et al.   JOURNAL OF BIOLOGICAL CHEMISTRY