HERC5 and the ISGylation Pathway: Critical Modulators of the Antiviral Immune Response

Statins attenuate antiviral IFN‐β and ISG expression via inhibition of IRF3 and JAK/STAT signaling in poly(I:C)‐treated hyperlipidemic mice and macrophages

(2021) Atsushi Koike et al.   FEBS Journal

Viral Evasion of RIG-I-Like Receptor-Mediated Immunity through Dysregulation of Ubiquitination and ISGylation

(2021) Cindy Chiang et al.   Viruses-Basel

Targeting XPO1 enhances innate immune response and inhibits KSHV lytic replication during primary infection by nuclear stabilization of the p62 autophagy adaptor protein

(2021) Wen Meng et al.   Cell Death & Disease

The complex structure of GRL0617 and SARS-CoV-2 PLpro reveals a hot spot for antiviral drug discovery

(2021) Ziyang Fu et al.   Nature Communications

EGCG, a green tea polyphenol, inhibits human coronavirus replication in vitro

(2021) Minsu Jang et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Inhibition of interferon-stimulated gene 15 and lysine 48-linked ubiquitin binding to the SARS-CoV-2 papain-like protease by small molecules: In silico studies

(2021) Eleni Pitsillou et al.   CHEMICAL PHYSICS LETTERS

High-throughput screening identifies established drugs as SARS-CoV-2 PLpro inhibitors

(2021) Yao Zhao et al.   Protein & Cell

ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity

(2021) GuanQun Liu et al.   Nature Microbiology

Quantification of Viral and Host Biomarkers in the Liver of Rhesus Macaques

(2020) Alexandra Greenberg et al.   AMERICAN JOURNAL OF PATHOLOGY

Deubiquitinating Enzymes in Coronaviruses and Possible Therapeutic Opportunities for COVID-19

(2020) Valentino Clemente et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

HECT E3 ubiquitin ligases – emerging insights into their biological roles and disease relevance

(2020) Yaya Wang et al.   JOURNAL OF CELL SCIENCE

Impairment of the deISGylation activity of FMDV Lpro causes attenuation in vitro and in vivo

(2020) Gisselle N. Medina et al.   JOURNAL OF VIROLOGY

Systemic Type I IFN Inflammation in Human ISG15 Deficiency Leads to Necrotizing Skin Lesions

(2020) Marta Martin-Fernandez et al.   Cell Reports

ISGylation of hepatitis C virus NS5A protein promotes viral RNA replication via the recruitment of cyclophilin A

(2020) Takayuki Abe et al.   JOURNAL OF VIROLOGY

Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity

(2020) Donghyuk Shin et al.   NATURE

Modulation of Extracellular ISG15 Signaling by Pathogens and Viral Effector Proteins

(2020) Caleb D. Swaim et al.   Cell Reports

Interferon stimulated gene 15 promotes Zika virus replication through regulating Jak/STAT and ISGylation pathways

(2020) Yancui Wang et al.   VIRUS RESEARCH

ISG15 Connects Autophagy and IFN-γ-Dependent Control of Toxoplasma gondii Infection in Human Cells

(2020) Jaya Bhushan et al.   mBio

ISG15 overexpression compensates the defect of Crimean-Congo hemorrhagic fever virus polymerase bearing a protease-inactive ovarian tumor domain

(2020) Stephanie Devignot et al.   PLoS Neglected Tropical Diseases

Flipping the substrate preference of Hazara virus ovarian tumour domain protease through structure-based mutagenesis

(2020) John V. Dzimianski et al.   Acta Crystallographica Section D-Structural Biology

Probing the impact of nairovirus genomic diversity on viral ovarian tumor domain protease (vOTU) structure and deubiquitinase activity

(2019) John V. Dzimianski et al.   PLoS Pathogens

Interferon-stimulated gene 15 (ISG15) restricts Zika virus replication in primary human corneal epithelial cells

(2019) Pawan Kumar Singh et al.   Ocular Surface

E3 ubiquitin ligases, the powerful modulator of innate antiviral immunity

(2019) Yi Zheng et al.   CELLULAR IMMUNOLOGY

HECT E3 Ligases: A Tale With Multiple Facets

(2019) Janine Weber et al.   Frontiers in Physiology

Stable Occupancy of the Crimean-Congo Hemorrhagic Fever Virus-Encoded Deubiquitinase Blocks Viral Infection

(2019) Florine E. M. Scholte et al.   mBio

Phosphorylation of MAVS/VISA by Nemo-like kinase (NLK) for degradation regulates the antiviral innate immune response

(2019) Shang-Ze Li et al.   Nature Communications

Decoupling deISGylating and deubiquitinating activities of the MERS virus papain-like protease

(2019) Jozlyn R. Clasman et al.   ANTIVIRAL RESEARCH

The in vivo ISGylome links ISG15 to metabolic pathways and autophagy upon Listeria monocytogenes infection

(2019) Yifeng Zhang et al.   Nature Communications

Determining the molecular drivers of species-specific interferon-stimulated gene product 15 interactions with nairovirus ovarian tumor domain proteases

(2019) John V. Dzimianski et al.   PLoS One

Structural mechanisms of HECT-type ubiquitin ligases

(2018) Sonja Lorenz   BIOLOGICAL CHEMISTRY

Evolution-Guided Structural and Functional Analyses of the HERC Family Reveal an Ancient Marine Origin and Determinants of Antiviral Activity

(2018) Ermela Paparisto et al.   JOURNAL OF VIROLOGY

Transmembrane Protein pUL50 of Human Cytomegalovirus Inhibits ISGylation by Downregulating UBE1L

(2018) Myoung Kyu Lee et al.   JOURNAL OF VIROLOGY

ISG15 in antiviral immunity and beyond

(2018) Yi-Chieh Perng et al.   NATURE REVIEWS MICROBIOLOGY

ISG'ylation increases stability of numerous proteins including Stat1, which prevents premature termination of immune response in LPS-stimulated microglia

(2018) Piotr Przanowski et al.   NEUROCHEMISTRY INTERNATIONAL

Irreversible inactivation of ISG15 by a viral leader protease enables alternative infection detection strategies

(2018) Kirby N. Swatek et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

BRCA1 Mutation Status and Follicular Fluid Exposure Alters NFκB Signaling and ISGylation in Human Fallopian Tube Epithelial Cells

(2018) Julia Hollingsworth et al.   NEOPLASIA

Overexpression of cellular telomerase RNA enhances virus-induced cancer formation

(2018) Ahmed Kheimar et al.   ONCOGENE

Restriction of Human Cytomegalovirus Replication by ISG15, a Host Effector Regulated by cGAS-STING Double-Stranded-DNA Sensing

(2017) Christopher Bianco et al.   JOURNAL OF VIROLOGY

Crimean-Congo Hemorrhagic Fever Virus Suppresses Innate Immune Responses via a Ubiquitin and ISG15 Specific Protease

(2017) Florine E.M. Scholte et al.   Cell Reports

Potent and selective inhibition of pathogenic viruses by engineered ubiquitin variants

(2017) Wei Zhang et al.   PLoS Pathogens

ISG15 governs mitochondrial function in macrophages following vaccinia virus infection

(2017) Sara Baldanta et al.   PLoS Pathogens

Chaperone-Mediated Autophagy Protein BAG3 Negatively Regulates Ebola and Marburg VP40-Mediated Egress

(2017) Jingjing Liang et al.   PLoS Pathogens

RLR-mediated antiviral innate immunity requires oxidative phosphorylation activity

(2017) Takuma Yoshizumi et al.   Scientific Reports

In vivo Ebola virus infection leads to a strong innate response in circulating immune cells

(2016) Ignacio S. Caballero et al.   BMC GENOMICS

Biochemical and Structural Insights into the Preference of Nairoviral DeISGylases for Interferon-Stimulated Gene Product 15 Originating from Certain Species

(2016) M. K. Deaton et al.   JOURNAL OF VIROLOGY

Mechanism of Lysine 48 Selectivity during Polyubiquitin Chain Formation by the Ube2R1/2 Ubiquitin-Conjugating Enzyme

(2016) Spencer Hill et al.   MOLECULAR AND CELLULAR BIOLOGY

TAX1BP1 Restrains Virus-Induced Apoptosis by Facilitating Itch-Mediated Degradation of the Mitochondrial Adaptor MAVS

(2016) Young Bong Choi et al.   MOLECULAR AND CELLULAR BIOLOGY

ISG15 deficiency and increased viral resistance in humans but not mice

(2016) Scott D. Speer et al.   Nature Communications

Influenza B virus non-structural protein 1 counteracts ISG15 antiviral activity by sequestering ISGylated viral proteins

(2016) Chen Zhao et al.   Nature Communications

Ubc13: the Lys63 ubiquitin chain building machine

(2016) Curtis D. Hodge et al.   Oncotarget

Transcriptomic analysis of KSHV-infected primary oral fibroblasts: The role of interferon-induced genes in the latency of oncogenic virus

(2016) Lu Dai et al.   Oncotarget

Consecutive Inhibition of ISG15 Expression and ISGylation by Cytomegalovirus Regulators

(2016) Ye Ji Kim et al.   PLoS Pathogens

The Role of HPV in Head and Neck Cancer Stem Cell Formation and Tumorigenesis

(2016) Mark Swanson et al.   Cancers

Structural and mutational analysis of the interaction between the Middle-East respiratory syndrome coronavirus (MERS-CoV) papain-like protease and human ubiquitin

(2016) Jian Lei et al.   VIROLOGICA SINICA

The SARS-coronavirus papain-like protease: Structure, function and inhibition by designed antiviral compounds

(2015) Yahira M. Báez-Santos et al.   ANTIVIRAL RESEARCH

Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon

(2015) Daichao Xu et al.   Autophagy

A comparative analysis of host responses to avian influenza infection in ducks and chickens highlights a role for the interferon-induced transmembrane proteins in viral resistance

(2015) Jacqueline Smith et al.   BMC GENOMICS

Inhibition of hepatitis C virus RNA replication by ISG15 does not require its conjugation to protein substrates by the HERC5 E3 ligase

(2015) Patricia Domingues et al.   JOURNAL OF GENERAL VIROLOGY

Kaposi's Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor 1 Interacts with a Member of the Interferon-Stimulated Gene 15 Pathway

(2015) Sarah R. Jacobs et al.   JOURNAL OF VIROLOGY

Biology of Zika Virus Infection in Human Skin Cells

(2015) Rodolphe Hamel et al.   JOURNAL OF VIROLOGY

Endothelial Cell Proteomic Response toRickettsia conoriiInfection Reveals Activation of the Janus Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT)-Inferferon Stimulated Gene (ISG)15 Pathway and Reprogramming Plasma Membrane Integrin/Cadherin Signaling

(2015) Yingxin Zhao et al.   MOLECULAR & CELLULAR PROTEOMICS

The HIV-1 Rev response element (RRE) adopts alternative conformations that promote different rates of virus replication

(2015) Chringma Sherpa et al.   NUCLEIC ACIDS RESEARCH

Phosphorylation of innate immune adaptor proteins MAVS, STING, and TRIF induces IRF3 activation

(2015) S. Liu et al.   SCIENCE

Identification and characterization of a novel ISG15-ubiquitin mixed chain and its role in regulating protein homeostasis

(2015) Jun-Bao Fan et al.   Scientific Reports

Isg15 controls p53 stability and functions

(2014) Yi-Fu Huang et al.   CELL CYCLE

A Novel Mitochondrial MAVS/Caspase-8 Platform Links RNA Virus-Induced Innate Antiviral Signaling to Bax/Bak-Independent Apoptosis

(2014) S. El Maadidi et al.   JOURNAL OF IMMUNOLOGY

Human intracellular ISG15 prevents interferon-α/β over-amplification and auto-inflammation

(2014) Xianqin Zhang et al.   NATURE

Interferon-induced HERC5 is evolving under positive selection and inhibits HIV-1 particle production by a novel mechanism targeting Rev/RRE-dependent RNA nuclear export

(2014) Matthew Woods et al.   Retrovirology

Viral OTU Deubiquitinases: A Structural and Functional Comparison

(2014) Ben A. Bailey-Elkin et al.   PLoS Pathogens

Structural Basis for the Ubiquitin-Linkage Specificity and deISGylating Activity of SARS-CoV Papain-Like Protease

(2014) Kiira Ratia et al.   PLoS Pathogens

IFNβ-dependent increases in STAT1, STAT2, and IRF9 mediate resistance to viruses and DNA damage

(2013) HyeonJoo Cheon et al.   EMBO JOURNAL

ISG15 Is Counteracted by Vaccinia Virus E3 Protein and Controls the Proinflammatory Response against Viral Infection

(2013) B. Eduardo-Correia et al.   JOURNAL OF VIROLOGY

Analysis of the Human Tissue-specific Expression by Genome-wide Integration of Transcriptomics and Antibody-based Proteomics

(2013) Linn Fagerberg et al.   MOLECULAR & CELLULAR PROTEOMICS

Structure of a ubiquitin-loaded HECT ligase reveals the molecular basis for catalytic priming

(2013) Elena Maspero et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Interferon-induced ISG15 pathway: an ongoing virus–host battle

(2013) Chen Zhao et al.   TRENDS IN MICROBIOLOGY

Correction: IRF-3, IRF-5, and IRF-7 Coordinately Regulate the Type I IFN Response in Myeloid Dendritic Cells Downstream of MAVS Signaling

(2013) Helen M. Lazear et al.   PLoS Pathogens

Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3

(2013) Hari B Kamadurai et al.   eLife

The RIG-I/MAVS signaling pathway in cancer cell-selective apoptosis

(2013) Yasufumi Kaneda   OncoImmunology

The mitochondrial pathway and reactive oxygen species are critical contributors to interferon-α/β-mediated apoptosis in Ubp43-deficient hematopoietic cells

(2012) Hwa Young Yim et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Differential Responses of Immune Cells to Type I Interferon Contribute to Host Resistance to Viral Infection

(2012) Thomas Baranek et al.   Cell Host & Microbe

K48-linked ubiquitination and protein degradation regulate 53BP1 recruitment at DNA damage sites

(2012) Frédérick A Mallette et al.   CELL RESEARCH

HERC6 Is the Main E3 Ligase for Global ISG15 Conjugation in Mouse Cells

(2012) Diede Oudshoorn et al.   PLoS One

Over-expression of mitochondrial antiviral signaling protein inhibits coxsackievirus B3 infection by enhancing type-I interferons production

(2012) Qing-Meng Zhang et al.   Virology Journal

Structure of the HECT:ubiquitin complex and its role in ubiquitin chain elongation

(2011) Elena Maspero et al.   EMBO REPORTS

Human dendritic cells promote an antiviral immune response when stimulated by CVT-E002

(2011) Ramses Ilarraza et al.   JOURNAL OF PHARMACY AND PHARMACOLOGY

Systematic and Quantitative Assessment of the Ubiquitin-Modified Proteome

(2011) Woong Kim et al.   MOLECULAR CELL

Molecular basis for ubiquitin and ISG15 cross-reactivity in viral ovarian tumor domains

(2011) M. Akutsu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease

(2011) T. W. James et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Human HERC5 restricts an early stage of HIV-1 assembly by a mechanism correlating with the ISGylation of Gag

(2011) Matthew W Woods et al.   Retrovirology

ISG15 facilitates cellular antiviral response to dengue and west nile virus infection in vitro

(2011) Jianfeng Dai et al.   Virology Journal

Inhibition of Hepatitis C Virus Replication by IFN-Mediated ISGylation of HCV-NS5A

(2010) M.-J. Kim et al.   JOURNAL OF IMMUNOLOGY

Herc5 Attenuates Influenza A Virus by Catalyzing ISGylation of Viral NS1 Protein

(2010) Y. Tang et al.   JOURNAL OF IMMUNOLOGY

Species-Specific Antagonism of Host ISGylation by the Influenza B Virus NS1 Protein

(2010) G. A. Versteeg et al.   JOURNAL OF VIROLOGY

The PB2 Subunit of the Influenza Virus RNA Polymerase Affects Virulence by Interacting with the Mitochondrial Antiviral Signaling Protein and Inhibiting Expression of Beta Interferon

(2010) K. M. Graef et al.   JOURNAL OF VIROLOGY

Positive Regulation of Interferon Regulatory Factor 3 Activation by Herc5 via ISG15 Modification

(2010) H. X. Shi et al.   MOLECULAR AND CELLULAR BIOLOGY

The ISG15 Conjugation System Broadly Targets Newly Synthesized Proteins: Implications for the Antiviral Function of ISG15

(2010) Larissa A. Durfee et al.   MOLECULAR CELL

ISG15 conjugation system targets the viral NS1 protein in influenza A virus-infected cells

(2010) C. Zhao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Antiviral Properties of ISG15

(2010) Deborah J. Lenschow   Viruses-Basel

Molecular discrimination of structurally equivalent Lys 63-linked and linear polyubiquitin chains

(2009) David Komander et al.   EMBO REPORTS

A Structural Element within the HUWE1 HECT Domain Modulates Self-ubiquitination and Substrate Ubiquitination Activities

(2009) Renuka K. Pandya et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Antiviral Activity of Innate Immune Protein ISG15

(2009) Ronald N. Harty et al.   Journal of Innate Immunity

Insights into Ubiquitin Transfer Cascades from a Structure of a UbcH5B∼Ubiquitin-HECTNEDD4L Complex

(2009) Hari B. Kamadurai et al.   MOLECULAR CELL

Unphosphorylated STAT1 prolongs the expression of interferon-induced immune regulatory genes

(2009) H. Cheon et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Acute induction of cell death-related IFN stimulated genes (ISG) differentiates highly from moderately virulent CSFV strains

(2009) Patricia Renson et al.   VETERINARY RESEARCH

Characterization of Polyubiquitin Chain Structure by Middle-down Mass Spectrometry

(2008) Ping Xu et al.   ANALYTICAL CHEMISTRY

The RCC1 superfamily: From genes, to function, to disease

(2008) Ouadah Hadjebi et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Regulation of the Nuclear Factor (NF)-κB Pathway by ISGylation

(2008) Miki Minakawa et al.   BIOLOGICAL & PHARMACEUTICAL BULLETIN

ISG15 Inhibits Nedd4 Ubiquitin E3 Activity and Enhances the Innate Antiviral Response

(2008) Oxana A. Malakhova et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Whole-Genome Expression Profiling Reveals That Inhibition of Host Innate Immune Response Pathways by Ebola Virus Can Be Reversed by a Single Amino Acid Change in the VP35 Protein

(2008) A. L. Hartman et al.   JOURNAL OF VIROLOGY

ISG15 inhibits Ebola VP40 VLP budding in an L-domain-dependent manner by blocking Nedd4 ligase activity

(2008) A. Okumura et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Vaccinia Virus E3 Protein Prevents the Antiviral Action of ISG15

(2008) Susana Guerra et al.   PLoS Pathogens

UbcH8 regulates ubiquitin and ISG15 conjugation to RIG-I

(2007) Kei-Ichiro Arimoto et al.   MOLECULAR IMMUNOLOGY