Article
Oncology
Zhuo Zhang, Max Costa
Summary: Metals can be toxic and carcinogenic to humans, with ROS playing a key role in metal carcinogenesis. Autophagy is crucial for maintaining cellular homeostasis, with p62 serving as a pivotal molecule in various cellular functions related to cancer growth.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Huizhen Lv, Ding Ai
Summary: Mechanical signals are translated into cell-specific transcriptional processes through cellular mechanic transducers, assisting in activated intracellular signal transduction pathways and phenotypic adaptations. The Hippo/YAP pathway is crucial in mechanotransduction, modulating gene expression in response to various mechanical stimuli to coordinate cell behavior and disease.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yuqi Qin, Daqi Yu, Dan Wu, Jiangqing Dong, William Thomas Li, Chang Ye, Kai Chit Cheung, Yingyi Zhang, Yun Xu, YongQiang Wang, Yun Stone Shi, Shangyu Dang
Summary: This study determines the cryo-EM structure of mouse TMEM63C and reveals its structural differences compared to other TMEM63 proteins. The coupling of TM0 and TM6 is shown to play important roles in channel activity. Additionally, it is discovered that TMEM63C exists primarily as a monomer, while TMEM63B can exist as a mixture of monomer and dimer, suggesting oligomerization as a regulatory mechanism.
NATURE COMMUNICATIONS
(2023)
Article
Cell & Tissue Engineering
Manuel A. Riquelme, Sumin Gu, Rui Hua, Jean X. Jiang
Summary: Mechanical loading activates integrin alpha 5 and alpha V, leading to the opening of connexin 43 (Cx43) hemichannels (HCs) and release of bone anabolic factors. Inhibition of integrin alpha V affects HCs opening and disrupts PI3K/AKT signaling pathway, while integrin alpha 5 activation is dependent on PI3K/AKT activation. Dendritic alpha V is a more sensitive mechanosensor than alpha 5 in activating HCs at low fluid shear stress levels.
Article
Multidisciplinary Sciences
Xing -Xing Dai, Shuai-Bo Pi, Long -Wen Zhao, Yun-Wen Wu, Jing-Ling Shen, Song-Ying Zhang, Qian-Qian Sha, Heng-Yu Fan
Summary: This study reveals the formation of non-membrane-bound nuclear poly(A) domains (NPAD) as the hub for newly transcribed mRNA in developing mouse oocytes. These NPAD are essential for the development of oocytes and female reproduction.
Article
Oncology
Rong Chen, Meng Zhao, Yanli An, Dongfang Liu, Qiusha Tang
Summary: This study identified potential therapeutic targets for hepatocellular carcinoma (HCC) through bioinformatics analysis and experimental validation, with the long noncoding RNA GBAP1 confirmed as a candidate prognostic biomarker and therapeutic target with pro-oncogenic function in HCC.
Review
Engineering, Biomedical
Sing Wan Wong, Stephen Lenzini, Regina Giovanni, Katherine Knowles, Jae-Won Shin
Summary: Hydrogels are used to design synthetic matrices that capture salient features of matrix microenvironments to study and control cellular functions. Recent advances in understanding extracellular matrix biology and biomaterial design have revealed the powerful role of biophysical cues in cell biology, particularly in mesenchymal stromal cells. This review discusses how biophysical cues in native microenvironments influence the ability of MSCs to regulate immune cell production and functions, and how these cues can be leveraged to optimize MSC therapy for immunomodulation.
ACTA BIOMATERIALIA
(2021)
Article
Oncology
Ming Chi, Jiao Liu, Chenxue Mei, Yaxing Shi, Nanqi Liu, Xuefeng Jiang, Chang Liu, Nan Xue, Hong Hong, Jisheng Xie, Xun Sun, Bo Yin, Xin Meng, Biao Wang
Summary: This study identified BLCA-related genes and found that TEAD4 plays an important role in EMT dysregulation. Overexpression of TEAD4 is associated with poor prognosis, invasion, and metastasis of BLCA, mediated through the regulation of the PI3K/AKT pathway. Therefore, TEAD4 is not only an effective biomarker for predicting prognosis but also a potential target for the treatment of metastatic BLCA.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Oncology
Yannick von Grabowiecki, Vinaya Phatak, Lydia Aschauer, Patricia A. J. Muller
Summary: Rab11-FIP1 is a Rab effector protein involved in endosomal recycling and trafficking, with its expression correlating with tumorigenic and metastatic cell behavior. Mutant p53 has been shown to promote Rab11-FIP1 function, leading to increased invasive behavior and chemoresistance by regulating various proteins.
FRONTIERS IN ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Pauline Andrieux, Christophe Chevillard, Edecio Cunha-Neto, Joao Paulo Silva Nunes
Summary: Mitochondria are the energy powerhouse of the cell, playing essential roles in inflammation, pathogen response, and oxidative stress. Pathogens can exploit mitochondria to disrupt cellular functions by affecting oxidative phosphorylation mechanisms. Targeting mitochondria could be a promising therapeutic strategy for infectious and inflammatory diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Xin Chen, Xinxin Song, Jingbo Li, Ruoxi Zhang, Chunhua Yu, Zhuan Zhou, Jiao Liu, Siyan Liao, Daniel J. Klionsky, Guido Kroemer, Jinbao Liu, Daolin Tang, Rui Kang
Summary: This study identifies HPCAL1 as a novel autophagy receptor for the selective degradation of CDH2 during ferroptosis. Depletion of CDH2 increases susceptibility to ferroptotic death. The phosphorylation of HPCAL1 and the non-classical LC3-interacting region motif play key roles in the autophagic degradation of CDH2. A ferroptosis inhibitor is found to suppress HPCAL1 expression. Inhibition of HPCAL1 prevents ferroptosis-induced tumor suppression and pancreatitis in mouse models.
Review
Plant Sciences
Maria E. Alvarez, Arnould Savoure, Laszlo Szabados
Summary: Proline is a multifunctional amino acid that accumulates in plants under various stress conditions, playing a role in regulating osmotic pressure, energy status, nutrient availability, redox balance, and defense against pathogens. Its biosynthesis and catabolism are linked to photosynthesis and mitochondrial respiration, and it can also function as a signal regulating gene expression and metabolic processes. This amino acid plays an important role in maintaining cellular homeostasis, regulating plant development, and promoting stress acclimation.
TRENDS IN PLANT SCIENCE
(2022)
Article
Astronomy & Astrophysics
Xiao-Lan Liu, Jin-Long Xu, Jun-Jie Wang, Nai-Ping Yu, Chuan-Peng Zhang, Nan Li, Guo-Yin Zhang
Summary: The study explores gas kinematics and physical properties in the W33 complex and its surrounding filaments. A new hub-filament system is identified within the W33 complex, with gas flows along the filaments likely leading to the clustering and formation of clumps. This research suggests a correlation between clump formation and star formation in the region.
ASTRONOMY & ASTROPHYSICS
(2021)
Review
Cell Biology
Xiaolan Fan, Tiantian Huang, Yingdong Tong, Ziqiang Fan, Ziyue Yang, Deying Yang, Xueping Mao, Mingyao Yang
Summary: p62, also known as SQSTM1, is a crucial predictor of autophagic flux and plays a role in maintaining protein homeostasis in cells. It has the ability to resist oxidative stress, and its multiple domains allow it to interact with vital signaling pathways, influencing cell proliferation, apoptosis, and survival. Recent studies have also shown its involvement in the aging process.
AGEING RESEARCH REVIEWS
(2022)
Review
Cell Biology
Gerhard Wiche
Summary: This essay explores the role of plectin and its various isoforms in mediating intermediate filament network functions. It discusses how plectin acts as an IF recruiter and its global role as a modulator of IF functionality. The essay also presents a working model that describes a unifying mechanism underlying how plectin-IF networks control actomyosin-generated forces and contribute to mechanotransduction.
Article
Gastroenterology & Hepatology
Mickael Di-Luoffo, Sophie Pirenne, Thoueiba Saandi, Axelle Loriot, Claude Gerard, Nicolas Dauguet, Fatima Manzano-Nunez, Natalia Alves Souza Carvalhais, Florence Lamoline, Sabine Cordi, Katarzyna Konobrocka, Vitaline De Greef, Mina Komuta, Georg Halder, Patrick Jacquemin, Frederic P. Lemaigre
Summary: A mouse model combining Kras(G12D) expression in cholangiocytes with DDC diet-induced inflammation accurately mimics the development of iCCA, showing similar histological features to human IPNBs and activating signaling pathways seen in human iCCA. TNS4, stimulated by KRAS(G12D), plays a crucial role in promoting tumor progression in this model.
Article
Medicine, Research & Experimental
Benoit Thibault, Fernanda Ramos-Delgado, Elvire Pons-Tostivint, Nicole Therville, Celia Cintas, Silvia Arcucci, Stephanie Cassant-Sourdy, Gabriela Reyes-Castellanos, Marie Tosolini, Amelie V. Villard, Coralie Cayron, Romain Baer, Justine Bertrand-Michel, Delphine Pagan, Dina Ferreira Da Mota, Hongkai Yan, Chiara Falcomata, Fabrice Muscari, Barbara Bournet, Jean-Pierre Delord, Ezra Aksoy, Alice Carrier, Pierre Cordelier, Dieter Saur, Celine Basset, Julie Guillermet-Guibert
Summary: Studies have shown that the PI3K pathway and PI3Kα activation signature can predict the aggressiveness and prognosis of PDAC. Pharmacological inhibition of PI3Kα can delay tumor cell migratory behavior, preventing macro-metastatic evolution.
EMBO MOLECULAR MEDICINE
(2021)
Editorial Material
Gastroenterology & Hepatology
C. Cayron, S. Rigal, J. Guillermet-Guibert
Summary: Pancreatic ductal adenocarcinoma is characterized by low immunogenicity and high autophagy flux, contributing to tumor progression. Understanding and targeting these mechanisms are crucial for improving treatment outcomes and patient survival.
CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY
(2021)
Article
Developmental Biology
Mickael Di-Luoffo, Kenley Joule Pierre, Nicholas M. Robert, Marie-Joelle Girard, Jacques J. Tremblay
Summary: This study reveals a new physical and functional cooperation between nuclear receptors SF1 and COUP-TFII in Insl3 gene expression, providing new insights into the regulation of Insl3 gene expression in Leydig cells.
Article
Cell Biology
Carole Nicco, Marine Thomas, Julie Guillermet, Maryline Havard, Fanny Laurent-Tchenio, Ludivine Doridot, Francois Dautry, Frederic Batteux, Thierry Tchenio
Summary: Self-sustained quiescence (SSQ) is a stable but reversible non-proliferative cellular state that limits the cloning of cultured cancer cells. It has been found that low cell density and the use of drugs can induce cells into SSQ state. In SSQ state, the tumor growth rate of pancreatic adenocarcinoma cells slows down, but eventually transitions to a rapid growth phase. Identifying new agents targeting cancer cells in SSQ may improve patient survival.
Article
Biochemistry & Molecular Biology
Carline Chaves-Almagro, Johanna Auriau, Alizee Dortignac, Pascal Clerc, Hubert Lulka, Simon Deleruyelle, Fabrice Projetti, Jessica Nakhle, Audrey Frances, Judit Berta, Veronique Gigoux, Daniel Fourmy, Marlene Dufresne, Anne Gomez-Brouchet, Julie Guillermet-Guibert, Pierre Cordelier, Bernard Knibiehler, Ralf Jockers, Philippe Valet, Yves Audigier, Bernard Masri
Summary: This study investigated the expression and protumoral function of apelin and its receptor APJ in human pancreatic adenocarcinoma. It was found that apelin and APJ were co-expressed by tumor cells in the invasive stage, and apelin promoted cancer cell proliferation, migration, and glucose uptake. Blocking the apelin receptor reduced cancer cell proliferation and pancreatic tumor burden.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Benoit Thibault, Fernanda Ramos-Delgado, Julie Guillermet-Guibert
Summary: Phosphatidylinositol-3-kinase (PI3K) enzymes play a key role in intracellular signaling and vesicular trafficking pathways by producing signaling phosphoinositides at plasma and intracellular membranes. There are eight isoforms of PI3K divided into three classes, which have various functions in physiology and are often deregulated in cancer. Recent evidence has shown that PI3K isoforms of class I, II, and III play important roles in tumor biology and that PI3K inhibitors have anti-tumoral action in preclinical cancer models. Understanding the functions of class II and III isoforms can lead to the development of combination treatment strategies targeting multiple PI3K isoforms simultaneously.
Review
Biology
Celine Schmitter, Mickael Di-Luoffo, Julie Guillermet-Guibert
Summary: In living organisms, cells can sense mechanical forces and respond to them through a process called mechanotransduction. Recent studies on human cells have shown that compressive forces can selectively regulate cell behavior, both in compressed and neighboring cells. This review summarizes the current knowledge of compression-induced cell signaling pathways and their effects in both physiological and pathological conditions, such as solid cancers.
LIFE SCIENCE ALLIANCE
(2023)
Article
Biochemical Research Methods
Zacchari Ben Meriem, Tiphaine Mateo, Julien Faccini, Celine Denais, Romane Dusfour-Castan, Catherine Guynet, Tatiana Merle, Magali Suzanne, Mickael Di-Luoffo, Julie Guillermet-Guibert, Baptiste Alric, Sylvain Landiech, Laurent Malaquin, Fabien Mesnilgrente, Adrian Laborde, Laurent Mazenq, Remi Courson, Morgan Delarue
Summary: Conventional culture conditions lack dynamic control over the microenvironment, hindering the study of tissues and organisms. In this study, a microfluidic device with sliding elements is presented, allowing for the control of both chemical and mechanical compressive forces. By confining micro-tissues, the impact of growth-induced pressure and macromolecular crowding was studied, shedding light on an understudied type of mechanical stress.
Article
Biochemical Research Methods
Zacchari Ben Meriem, Tiphaine Mateo, Julien Faccini, Celine Denais, Romane Dusfour-Castan, Catherine Guynet, Tatiana Merle, Magali Suzanne, Mickael Di-Luoffo, Julie Guillermet-Guibert, Baptiste Alric, Sylvain Landiech, Laurent Malaquin, Fabien Mesnilgrente, Adrian Laborde, Laurent Mazenq, Remi Courson, Morgan Delarue
Summary: Conventional culture conditions lack dynamic chemical and mechanical control over the microenvironment, making them insufficient for studying tissues, organisms, or 3D multicellular assemblies. This study presents a microfluidic device that allows control of both chemical and mechanical compressive forces, enabling the study of biological samples under specific conditions and the impact of mechanical compression. The device utilizes sliding elements to create reconfigurable closed culture chambers for the study of whole organisms or model micro-tissues.
Meeting Abstract
Gastroenterology & Hepatology
Mickael Di-Luoffo, Sophie Pirenne, Thoueiba Saandi, Axelle Loriot, Claude Gerard, Nicolas Dauguet, Fatima Manzano-Nunez, Natalia Alves Souza Carvalhais, Florence Lamoline, Sabine Cordi, Katarzyna Konobrocka, Vitaline De Greef, Mina Komuta, Georg Halder, Patrick Jacquemin, Frederic Lemaigre
JOURNAL OF HEPATOLOGY
(2021)