Article
Chemistry, Analytical
Daniel H. Lysak, William W. Wolff, Ronald Soong, Wolfgang Bermel, Eriks Kupce, Amy Jenne, Rajshree Ghosh Biswas, Daniel Lane, Genevieve Gasmi-Seabrook, Andre Simpson
Summary: Many important biological components contain nitrogen. However, N-15 nuclei are rarely used in NMR-based metabolite assignment due to their low abundance and the lack of comprehensive databases. In this study, a broadband N-15-edited H-1-C-13 HSQC NMR experiment was developed to identify nitrogen-containing metabolites based on their C-13, H-1, and N-15 chemical shifts.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Chemical
Neil Robinson, Razyq Nasharuddin, Ganhua Luo, Andy Fourie, Einar O. Fridjonsson, Michael L. Johns
Summary: The study used H-1 NMR T-1-T-2 correlation measurements to investigate the hydration behavior of CPB samples, revealing complex and rapidly evolving hierarchical pore networks. Relaxation peaks were assigned to a combination of pore water populations and pore-to-pore exchange dynamics with the aid of additional relaxation-exchange correlation measurements. The hydration behavior of hypersaline and tap-water-hydrated materials were further discussed in terms of observed exchange processes and evolving pore surface chemistry.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2021)
Article
Neurosciences
Qianyun Chen, Jill Abrigo, Wanting Liu, Elyia Yixun Han, David Ka Wai Yeung, Lin Shi, Lisa Wing Chi Au, Min Deng, Sirong Chen, Eric Yim Lung Leung, Chi Lai Ho, Vincent Chung Tong Mok, Winnie Chiu Wing Chu
Summary: This study aimed to determine the proton MR spectroscopic patterns of the posterior cingulate in individuals with Alzheimer's disease (AD). The findings showed that early AD patients had lower N-acetylaspartate (NAA)/creatine (Cr) levels compared to controls, while late AD patients had lower NAA/Cr and higher myoInositol (mI)/Cr levels. Lower NAA/Cr levels were associated with a greater global amyloid and tau load and allowed for the differentiation of AD patients from controls.
Article
Neurosciences
Fangyuan Liang, Lei Du, Xiaoping Rao, Ying Li, Wei Long, Jiaxuan Tian, Xuanai Zhu, Aijia Zou, Wei Lu, Bijiang Wan
Summary: This study investigates the effect of electroacupuncture (EA) at the Zusanli acupoint on a rat model of rheumatoid arthritis (RA). The results show that EA effectively alleviates RA symptoms and has an impact on the metabolic activity of the brain. The study suggests that GABAergic and glutamatergic neurons in the midbrain, along with astrocytes in the hippocampus, play important roles in the beneficial effects of EA on RA.
BRAIN RESEARCH BULLETIN
(2023)
Article
Medicine, General & Internal
Bo Yang, Chuan Zhang, Sheng Cheng, Gonghui Li, Jan Griebel, Jochen Neuhaus
Summary: This study identified guanidinoacetate, phenylacetylglycine, and glycine as potential candidate biomarkers for prostate cancer, with the ability to accurately differentiate patients and show potential differences among different patient subgroups. Pathway enrichment analysis also revealed the potential involvement of Glycine, Serine, and Threonine metabolism in prostate cancer.
Article
Chemistry, Analytical
Philipp Nitschke, Samantha Lodge, Drew Hall, Hartmut Schaefer, Manfred Spraul, Nieves Embade, Oscar Millet, Elaine Holmes, Julien Wist, Jeremy K. Nicholson
Summary: A JEDI NMR pulse experiment was conducted on a low field spectrometer system to quantitatively measure two plasma markers of SARS-CoV-2 infection and inflammation. The experiment successfully captured the unique biomarker signals and showed comparable results to high field machines. Simplification of sample preparation allowed for shorter testing time and high-throughput capability.
Article
Multidisciplinary Sciences
L. Nie, A. C. Nusantara, V. G. Damle, R. Sharmin, E. P. P. Evans, S. R. Hemelaar, K. J. van der Laan, R. Li, F. P. Perona Martinez, T. Vedelaar, M. Chipaux, R. Schirhagl
Summary: Relaxometry measurement inherited from diamond magnetometry can be used to detect free radicals in living cells with subcellular resolution. This quantum sensing technique based on defects in diamond allows nanoscale magnetic resonance measurements to detect metabolic activity in mitochondria.
Article
Agriculture, Dairy & Animal Science
G. Niero, G. Meoni, L. Tenori, C. Luchinat, G. Visentin, S. Callegaro, E. Visentin, M. Cassandro, M. De Marchi, M. Penasa
Summary: This study investigated the use of proton nuclear magnetic resonance spectroscopy to discriminate between milk produced before and after the grazing season, and to assess the effects of grazing on milk metabolites. The results showed that the H-1 NMR spectra of milk were promising for identifying milk produced under different conditions. Additionally, grazing was found to significantly impact the metabolic profile of milk, suggesting the potential use of several metabolites as indicators of farm management.
JOURNAL OF DAIRY SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Jonathan Chabert, Etienne Allauze, Bruno Pereira, Carine Chassain, Ingrid De Chazeron, Jean-Yves Rotge, Philippe Fossati, Pierre-Michel Llorca, Ludovic Samalin
Summary: The levels of neurometabolites such as Glutamate, Glutamine, Glx, and N-acetylaspartate in various brain regions differ in patients with bipolar disorder. The altered levels of NAA and Gln may be associated with the pathogenesis of bipolar disorder.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Clinical Neurology
J. N. Bissonnette, A. M. Francis, S. MacNeil, C. E. Crocker, P. G. Tibbo, D. J. Fisher
Summary: This systematic review provides an overview of the research on glutamate and N-acetylaspartate concentrations in multiple brain regions of individuals with early phase psychosis. Most studies suggest no significant alterations in glutamate and N-acetylaspartate concentrations in early phase psychosis. Future research should focus on less commonly reported brain regions and explore possible confounding factors.
PSYCHIATRY RESEARCH-NEUROIMAGING
(2022)
Article
Food Science & Technology
Poliana Cristina Spricigo, Luisa Souza Almeida, Gabriel Henrique Ribeiro, Banny Silva Barbosa Correia, Isabela Barroso Taver, Angelo Pedro Jacomino, Luiz Alberto Colnago
Summary: The metabolites of uvaia have been determined using nuclear magnetic resonance spectroscopy. A total of 26 metabolites, including sugars, acids, and amino acids, were identified. Uvaia exhibits rich nutritional composition, diverse physicochemical attributes, and has potential applications in various economic sectors such as food and pharmaceuticals.
Article
Psychiatry
Vera J. Erchinger, Alexander R. Craven, Lars Ersland, Ketil J. Oedegaard, Christoffer A. Bartz-Johannessen, Asa Hammar, Jan Haavik, Frank Riemer, Ute Kessler, Leif Oltedal
Summary: Based on previous research, we propose a model where disruption, potentiation, and rewiring of brain networks occur in sequence and serve as the therapeutic mechanism of electroconvulsive therapy (ECT). Our results suggest that tNAA levels decrease after ECT treatment but return to baseline after 6 months, supporting the disrupt, potentiate, and rewire model.
FRONTIERS IN PSYCHIATRY
(2023)
Article
Agriculture, Dairy & Animal Science
Yuxi Song, Hongyu Wang, Rui Sun, Jinshui Chang, Jipeng Tang, Yunlong Bai, Cheng Xia
Summary: Vitamin E deficiency (VED) in cows during the transition period can lead to oxidative stress, immunological dysfunction, and increased risk of peripartum disorders. This study used H-1 nuclear magnetic resonance (H-1 NMR) to analyze the alterations in serum metabolic activities of VED cows. The results showed significant differences in body condition scores, milk yield, and dry matter intake between VED cows and normal cows. Higher levels of certain substances and lower levels of others were found in the blood samples of VED cows. Furthermore, 24 upregulated serum metabolites were identified under VED conditions, indicating changes in 11 metabolic pathways. This study provides valuable insights into the metabolic changes associated with VED in cows.
Article
Physics, Multidisciplinary
Tomomi Kawaguchi
Summary: The search for rare decays of the Standard Model Higgs boson and additional Higgs boson was conducted using data recorded by the ATLAS experiment at LHC, showing no significant deviation from the Standard Model. These searches provide a deeper understanding of the Higgs sector.
Article
Chemistry, Multidisciplinary
Luca Nagel, Martin Gierse, Wolfgang Gottwald, Zumrud Ahmadova, Martin Grashei, Pascal Wolff, Felix Josten, Senay Karaali, Christoph A. Mueller, Sebastian Lucas, Jochen Scheuer, Christoph Mueller, John Blanchard, Geoffrey J. Topping, Andre Wendlinger, Nadine Setzer, Sandra Suehnel, Jonas Handwerker, Christophoros Vassiliou, Frits H. A. van Heijster, Stephan Knecht, Michael Keim, Franz Schilling, Ilai Schwartz
Summary: Hyperpolarization techniques significantly increase nuclear spin polarization, enabling metabolic MRI. However, the complexity, cost, and slowness of current equipment limit its widespread use. In this study, a polarization procedure using parahydrogen-induced polarization by side-arm hydrogenation (PHIP-SAH) in an automated polarizer is demonstrated, which produces purified, concentrated, and highly hyperpolarized pyruvate solutions within a short time. The results show that PHIP is a viable alternative to established hyperpolarization techniques, with equivalent safety profile, image quality, and quantitative measurements.
Article
Neurosciences
Yue Liu, Shusheng Yang, Erli Cai, Li Lin, Peng Zeng, Binbin Nie, Fuqiang Xu, Qing Tian, Jie Wang
CNS NEUROSCIENCE & THERAPEUTICS
(2020)
Article
Biochemistry & Molecular Biology
Liang Wu, Zhanfeng Niu, Xulei Hu, Huili Liu, Shuang Li, Lei Chen, Danhao Zheng, Zhuang Liu, Taotao Liu, Fuqiang Xu, Anne Manyande, Jie Wang, Hechun Xia
Article
Biochemistry & Molecular Biology
Lei Chen, Shuang Li, Ying Zhou, Taotao Liu, Aoling Cai, Zongze Zhang, Fuqiang Xu, Anne Manyande, Jie Wang, Mian Peng
Summary: The study demonstrates the important roles played by A(2A) receptor in the loss of consciousness induced by propofol, suggesting that the neural circuit between NAc-DR may be controlled by A(2A)R in the mechanism of anesthesia induced by propofol.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Qian Xiao, Xinyi Zhou, Pengfei Wei, Li Xie, Yaning Han, Jie Wang, Aoling Cai, Fuqiang Xu, Jie Tu, Liping Wang
Summary: Parvalbumin interneurons within the nucleus accumbens shell are crucial in modulating anxiety-like avoidance behavior, potentially via GABAergic pathways. Chronic stress increases the excitability of these neurons, leading to excessive maladaptive avoidance responses. The coordination between different types of neurons and GABAergic afferents plays a key role in regulating anxiety-related behaviors, providing a potential neurobiological basis for therapeutic interventions in pathological anxiety.
MOLECULAR PSYCHIATRY
(2021)
Article
Engineering, Environmental
Mojtaba Ehsanifar, Ahmad Jonidi Jafari, Zeinab Montazeri, Roshanak Rezaei Kalantari, Mitra Gholami, Azadeh Ashtarinezhad
Summary: Exposure to diesel exhaust particles has been shown to increase oxidative stress, neuroinflammation, anxiety, and impair learning and memory in mice. Additionally, DEP exposure may elevate anxiety levels in male mice.
JOURNAL OF ENVIRONMENTAL HEALTH SCIENCE AND ENGINEERING
(2021)
Article
Medicine, Research & Experimental
Yuanyuan Qin, Jinfeng Wu, Tao Chen, Jia Li, Guiling Zhang, Di Wu, Yiran Zhou, Ning Zheng, Aoling Cai, Qin Ning, Anne Manyande, Fuqiang Xu, Jie Wang, Wenzhen Zhu
Summary: The study found that COVID-19 patients showed severe changes in gray matter, white matter, and cerebral blood flow, especially in patients with severe disease. These changes were significantly correlated with inflammatory markers, suggesting that the inflammatory storm may cause indirect brain damage.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Chemistry, Analytical
Danhao Zheng, Zhao Li, Shuang Li, Xihai Li, Ghulam Mustafa Kamal, Chaoyang Liu, Anne Manyande, Fuqiang Xu, Qingjia Bao, Jie Wang
Summary: Metabolomics is used to identify metabolic patterns and functions of organs and biological systems, with neuronal activity highly correlated with cerebral energy metabolism. Discriminant analysis was used to analyze metabolic kinetics in different brain regions, showing that each region has its own metabolic pattern. Boost-decision tree method was found to be the best for classification in this study.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Mojtaba Ehsanifar, Zeinab Montazeri, Maryam Akhavan Taheri, Mehravar Rafati, Mohaddeseh Behjati, Mohammad Karimian
Summary: Exposure to diesel exhaust particles (DEPs) can lead to neuroinflammation and oxidative stress in both female and male mice, with males showing a higher susceptibility compared to females. Long-term exposure to DEPs alters the expression of hippocampal NMDA receptor subunits, suggesting that gender may play a significant role in modulating susceptibility to neurotoxicity induced by DEPs exposure.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Environmental Sciences
Mojtaba Ehsanifar
Summary: Researchers are actively investigating the transmission of the coronavirus, with aerosol transmission considered possible. Precautionary control strategies to effectively reduce virus transmission need to be considered.
ENVIRONMENTAL RESEARCH
(2021)
Article
Environmental Sciences
Mojtaba Ehsanifar, Zeinab Yavari, Mehravar Rafati
Summary: Air pollution is linked to neurodevelopmental and neurological disorders. Rodent models have shown similar neurotoxic effects of urban air pollution particles from different sources. Exposure to ultrafine particulate matter may negatively impact the central nervous system through inflammation and oxidative stress. This study found that prolonged exposure to diesel exhaust particles can alter neurobehavioral functions and impair cognition.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Mojtaba Ehsanifar, Zeinab Montazeri, Mahmoud Salami Zavareh, Mehravar Rafati, Jie Wang
Summary: Long-term exposure to diesel exhaust particles (DEPs) can impair emotional and cognitive responses, increase the expression of hippocampal inflammatory cytokines, and lead to the activation of microglia.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Ting Hu, Quanfei Zhu, Yuning Hu, Ghulam Mustafa Kamal, Yuqi Feng, Anne Manyande, Jie Wang, Fuqiang Xu
Article
Oncology
Wenchang Zhou, Aoling Cai, Binbin Nie, Wen Zhang, Ting Yang, Ning Zheng, Anne Manyande, Xuxia Wang, Fuqiang Xu, Xuebi Tian, Jie Wang
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2020)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)