Review
Biochemistry & Molecular Biology
Yan Qin Tan, Xi Zhang, Shuwei Zhang, Tao Zhu, Manoj Garg, Peter E. Lobie, Vijay Pandey
Summary: Mitochondria, known as the powerhouse of cells, play essential roles in various cellular functions and are associated with human malignancies. Cancer development involves abnormalities in cellular programs, and mitochondria are recognized as key metabolic switches for normal cells to acquire malignant characteristics.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2021)
Review
Endocrinology & Metabolism
Aakriti Jain, Roberto Zoncu
Summary: This review discusses how cells coordinate metabolism and communication between organelles using membrane transporters, membrane contact sites, and localized signaling pathways. It also highlights the disruption of inter-organelle communication as an emerging driver in various diseases.
MOLECULAR METABOLISM
(2022)
Review
Biochemistry & Molecular Biology
Tina Podinic, Geoff Werstuck, Sandeep Raha
Summary: The endocannabinoid system (ECS) is an integrated signaling network that regulates various physiological processes and plays a crucial role in cellular growth and differentiation. Cannabinoid receptors are present on cellular and mitochondrial membranes and can be activated by different types of cannabinoids, making the ECS highly versatile. Evidence suggests that disruptions in the ECS can affect cellular differentiation and disrupt essential energy-sensing metabolic signaling pathways. This review aims to explore the extent of these disruptions and their implications for cellular differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Franziska Bierbuesse, Anais C. Bourges, Vincent Gielen, Viola Monkemoller, Wim Vandenberg, Yi Shen, Johan Hofkens, Pieter Vanden Berghe, Robert E. Campbell, Benjamien Moeyaert, Peter Dedecker
Summary: The authors present a method, called photochromism-enabled absolute quantification (PEAQ) biosensing, that utilizes the photochromic properties of biosensors to provide an absolute measure of analyte concentration or activity. By developing photochromic variants of the popular GCaMP family of Ca2+ biosensors, they are able to resolve dynamic changes in the absolute Ca2+ concentration in live cells. They also demonstrate how this method can be applied to other biosensors with photochromic properties, expanding the possibilities for fully quantitative measurements in complex and dynamic systems.
NATURE COMMUNICATIONS
(2022)
Review
Medicine, General & Internal
Tapan Behl, Rashita Makkar, Md. Khalid Anwer, Rym Hassani, Gulrana Khuwaja, Asaad Khalid, Syam Mohan, Hassan A. Alhazmi, Monika Sachdeva, Mahesh Rachamalla
Summary: Mitochondria are semiautonomous intracellular components involved in metabolism and energy production. Defects in mitochondria lead to chronic cellular diseases. Dysfunctional mitochondria play a critical role in cancer, diabetes, infections, and obesity.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Environmental Sciences
Ruijia Zhang, Baowei Chen, Li Lin, Hui Zhang, Tiangang Luan
Summary: The study found that perfluorooctanoic acid (PFOA) primarily affects glucose metabolism by altering the tricarboxylic acid (TCA) cycle rather than glycolysis, leading to impaired mitochondrial respiratory function. Metabolic flux analysis (MFA) is a promising tool for investigating the toxicity mechanisms of contaminants associated with glucose metabolism.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Minggang Tian, Enxiang Ge, Baoli Dong, Yujing Zuo, Yuping Zhao, Weiying Lin
Summary: A novel fluorescent probe MT-LD was designed in this study to label lipid droplets and mitochondria, allowing for the visualization of their interaction during processes such as oleic acid treatment and starvation-induced autophagy. The probe revealed an increase in mitochondria-LD contact and peridroplet mitochondria amount in different tissues, such as brown adipose tissue and lung tissue. This work provides a valuable tool for investigating the interplay between mitochondria and lipid droplets, promoting research in fatty acid metabolism and autophagy.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Luling Wu, Jihong Liu, Xue Tian, Robin R. Groleau, Beidou Feng, Yonggang Yang, Adam C. Sedgwick, Hai-Hao Han, Yang Wang, Han-Min Wang, Fang Huang, Steven D. Bull, Hua Zhang, Chusen Huang, Yi Zang, Jia Li, Xiao-Peng He, Ping Li, Bo Tang, Tony D. James, Jonathan L. Sessler
Summary: The fluorescent probe ATP-LW was developed for simultaneous detection of ONOO- and ATP levels. By utilizing different emission properties of the products, ONOO- levels can be monitored in the green channel and ATP concentrations in the red channel. The probe was successfully used in hepatocytes to demonstrate changes in signal intensity in response to ATP synthase inhibition and exogenous ONOO- exposure.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Biochemistry & Molecular Biology
Beste Mutlu, Pere Puigserver
Summary: GCN5, encoded by the mammalian gene Kat2a, is a histone acetyltransferase that plays a crucial role in regulating gene expression programs in response to cellular metabolic and energetic states. It functions as a nutrient sensor, linking histone acetylation to transcriptional activation, and also plays a significant role in energy metabolism by acetylating proteins like PGC-1 alpha.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2021)
Article
Chemistry, Analytical
Yao-Jia Ma, Si Li, Ya-Ting Qin, Xi-Wen He, Wen-You Li, Yu-Kui Zhang
Summary: A novel turn-on fluorescent silicon nanoparticle (SiNP) was developed for monitoring the pH and GSH changes in cells. The SiNPs, synthesized via a one-pot hydrothermal method, showed increased fluorescence intensity with increasing acidity, indicating excellent pH and GSH sensing capability. Confocal imaging and flow cytometry experiments confirmed the pH and GSH sensing performance of the SiNPs in cells. The prepared SiNPs have the potential to serve as an intracellular pH and GSH multimode fluorescent sensing platform, capable of distinguishing normal cells from cancer cells.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Yao-Wen Wu
Summary: New red-green optogenetic dimerizers allow precise control of gene expression activation and deactivation with high spatiotemporal resolution.
Article
Chemistry, Multidisciplinary
Mengyu Chang, Zhiyao Hou, Man Wang, Ding Wen, Chunxia Li, Yuhui Liu, Yanli Zhao, Jun Lin
Summary: In this study, a polyporous Cu single atom nanozyme loaded with licogliflozin (LIK066) was designed to achieve efficient mild photothermal therapy (PTT) by inhibiting heat shock proteins (HSPs) synthesis and damaging existing HSPs in cancer cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Multidisciplinary Sciences
Elisa B. Randi, Karim Zuhra, Laszlo Pecze, Theodora Panagaki, Csaba Szabo
Summary: In mammalian cells, cyanide exhibits a biphasic effect on mitochondrial Complex IV activity, stimulating it at low concentrations and inhibiting it at higher concentrations. Low levels of cyanide lead to increased cell proliferation and ATP production, while higher levels result in inhibition of cellular bioenergetics. The presence of cyanide-producing enzymes in mammalian cells suggests a regulatory role for endogenous cyanide.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Wenjin Cai, Jinglun Zhang, Yiqian Yu, Yueqi Ni, Yan Wei, Yihong Cheng, Litian Han, Leyi Xiao, Xiaoxin Ma, Hongjiang Wei, Yaoting Ji, Yufeng Zhang
Summary: Mitochondrial transfer from macrophages to mesenchymal stem cells regulates bone homeostasis, and abnormal transfer under osteoporotic conditions leads to metabolic remodeling in stem cells.
Article
Multidisciplinary Sciences
Jacqueline E. Lebenzon, Peter W. Denezis, Lamees Mohammad, Katherine E. Mathers, Kurtis F. Turnbull, James F. Staples, Brent J. Sinclair
Summary: A study has found that dormant Colorado potato beetles achieve metabolic suppression through mitophagy, which leads to the breakdown of flight muscle mitochondria. The beetles reverse mitophagy and increase mitochondrial biogenesis at the end of diapause to replenish their flight muscle mitochondrial pools.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Linyan Nie, Anggrek C. Nusantara, Viraj G. Damle, Maxim Baranov, Mayeul Chipaux, Claudia Reyes-San-Martin, Thamir Hamoh, Chandra Prakash Epperla, Miroslava Guricova, Petr Cigler, Geert van den Bogaart, Romana Schirhagl
Summary: Free radicals are important indicators for stress and pathogenic conditions, and are difficult to detect due to their reactivity and low abundance. A novel relaxometry method using a fluorescent defect in a diamond has been developed for subcellular detection of radicals with nanoscale MRI capabilities. Variability in radical production was observed in primary dendritic cells from healthy donors, and their response to stimulation or inhibition was investigated.
Article
Chemistry, Physical
Neda Norouzi, Anggrek Citra Nusantara, Yori Ong, Thamir Hamoh, Linyan Nie, Aryan Morita, Yue Zhang, Aldona Mzyk, Romana Schirhagl
Summary: The generation of free radicals in Staphylococcus aureus is found to increase in the presence of UV irradiation and vancomycin, depending on the antibiotic dose. By using a new method, researchers were able to track the process of free radical generation near individual bacteria and observe the concentration of free radicals within nanoscale voxels.
Article
Nanoscience & Nanotechnology
Yuchen Tian, Anggrek C. Nusantara, Thamir Hamoh, Aldona Mzyk, Xiaobo Tian, Felipe Perona Martinez, Runrun Li, Hjalmar P. Permentier, Romana Schirhagl
Summary: This study presents multifunctional fluorescent nanodiamonds (FNDs) that can simultaneously deliver drugs and detect free radicals. The modified FNDs were found to have enhanced cellular uptake and drug delivery capabilities. The sustained release of the drug by the FNDs was observed over a longer period compared to the free drug. Additionally, the FNDs allowed for direct measurement of the drug's effect on free radical generation.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Alina Sigaeva, Hoda Shirzad, Felipe Perona Martinez, Anggrek Citra Nusantara, Nikos Mougios, Mayeul Chipaux, Romana Schirhagl
Summary: Diamond magnetometry is a sensitive technique that converts magnetic resonance signals into fluorescence, allowing for nanometer-scale detection of free radical production in individual living cells. This method holds several sensitivity world records for magnetic measurements at room temperature.
Article
Chemistry, Multidisciplinary
Romana Schirhagl, Kaiqi Wu, Linyan Nie, Anggrek C. Nusantara, Willem Woudstra, Thea Vedelaar, Alina Sigaeva
Summary: Free radicals have an important role in antimicrobial activities, but macrophages sometimes fail to kill Staphylococcus aureus due to the bacteria's ability to withstand oxidative stress. This study used diamond-based quantum sensing to investigate changes in the free radical response near S. aureus in macrophages.
Article
Chemistry, Multidisciplinary
Aryan Morita, Anggrek C. Nusantara, Aldona Myzk, Felipe P. Perona Martinez, Thamir Hamoh, Viraj G. Damle, Kiran J. van der Laan, Alina Sigaeva, Thea Vedelaar, Michael Chang, Mayeul Chipaux, Romana Schirhagl
Summary: Free radicals play a crucial role in ageing, but their measurement is challenging due to their small size and short lifespan. In this study, we utilize a new technique called diamond magnetometry, using nitrogen vacancy centers in nanodiamonds, to measure free radicals in living cells for the first time. With this powerful tool, we are able to track free radical generation and reduction, differentiate mutant strains with altered metabolism, and observe the ageing process in cells.
Article
Chemistry, Multidisciplinary
Romana Schirhagl, Rokshana Sharmin, Anggrek C. Nusantara, Linyan Nie, Kaiqi Wu, Arturo Elias Llumbet, Willem Woudstra, Aldona Mzyk
Summary: Acetaminophen overdoses cause liver cell injury mainly due to the damage of mitochondrial proteins and nuclear DNA by the reactive metabolite NAPQI. This study employed relaxometry technique to measure the free radical load in different cellular compartments, revealing organelle-specific production of free radicals induced by APAP for the first time, which helps predict and understand cellular toxicity.
Review
Pharmacology & Pharmacy
Linyan Nie, Yuanfeng Li, Yong Liu, Linqi Shi, Huiyun Chen
Summary: This review examines the current applications of contact lenses for anti-corneal keratitis, discussing the development of contact lenses and the role of functionalized contact lenses.
Article
Chemistry, Multidisciplinary
S. Fan, L. Nie, Y. Zhang, E. Ustyantseva, W. Woudstra, H. H. Kampinga, R. Schirhagl
Summary: Huntington's disease is a rare disease caused by a specific mutation that results in the expression of polyglutamine. The study found that free radicals are generated near PolyQ aggregates at autolysosomes, which may be either a cause or consequence of the Huntington disease phenotype. The method of relaxometry and nanoscale MRI measurements revealed this relationship.
ACS CENTRAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Charles Mignon, Ari R. Ortiz Moreno, Hoda Shirzad, Sandeep K. Padamati, Viraj G. Damle, Yori Ong, Romana Schirhagl, Mayeul Chipaux
Summary: We propose an alternative method to using bulky magnets and magnetron equipment in conventional Electron Paramagnetic Resonance (EPR) spectroscopy. Instead, we utilize the photoluminescence of an ensemble of Nitrogen-Vacancy (NV) centers on a diamond surface. By monitoring the relaxation time (T1) of these centers, we are able to detect their cross-relaxation with a specific compound. Additionally, we encode the EPR spectra through a localized magnetic field gradient, enabling faster data acquisition and improved sensitivity.
Article
Materials Science, Multidisciplinary
Baptiste Vindolet, Marie -Pierre Adam, Loic Toraille, Mayeul Chipaux, Antoine Hilberer, Geraud Dupuy, Lukas Razinkovas, Audrius Alkauskas, Gergo Thiering, Adam Gali, Mary De Feudis, Midrel Wilfried Ngandeu Ngambou, Jocelyn Achard, Alexandre Tallaire, Martin Schmidt, Christoph Becher, Jean-Francois Roch
Summary: In this study, we investigate the optical properties of silicon-vacancy (SiV) and germanium-vacancy (GeV) color centers in nanodiamonds under high pressures. We observe blueshifts in their spectra, and these shifts are in good agreement with ab initio calculations considering the lattice compression. This research provides guidance for using group-IV-vacancy centers as quantum sensors with specific optical and spin properties under extreme pressures.
