Article
Chemistry, Multidisciplinary
Sarah H. Klass, Laura E. Sofen, Zachary F. Hallberg, Tahoe A. Fiala, Alexandra Ramsey, Nicholas S. Dolan, Matthew B. Francis, Ariel L. Furst
Summary: Pathogenic E. coli strains are a significant threat to public health, causing both foodborne illnesses and urinary tract infections. By using a rapid bioconjugation reaction and electrochemical impedance spectroscopy, we can selectively capture and accurately quantify these pathogenic microbes on a disposable gold electrode.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yuqing Zhou, Ziyong Li, Jijie Huang, Yuxing Wu, Xinyu Mao, Yizhou Tan, Hui Liu, Dongxin Ma, Xun Li, Xiaoye Wang
Summary: In this study, an electrochemical biosensor using phage EP01 as the recognition agent for detection of E. coli O157:H7 GXEC-N07 was established. The biosensor showed good linearity in the concentration range of 10(2) CFU/mL to 10(2) CFU/mL with a low detection limit of 11.8 CFU/mL, and the whole process was completed in less than 30 min. The biosensor was successfully applied to the quantitative detection of GXEC-N07 in fresh milk and raw pork. The recovery values ranged from 60.8% to 114.2%. The biosensor provides a rapid, specific, low cost, and label free tool for E. coli O157:H7 GXEC-N07 detection, and is expected to become a powerful method for the detection of bacteria threatening food safety and public health security.
BIOELECTROCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
So-Seum Yong, Jae-Ik Lee, Dong-Hyun Kang
Summary: This study investigated the photocatalytic activity of TiO2-based photocatalysts in inactivating foodborne pathogens in various environments. The results showed that Degussa P25 under UVA exhibited the highest bactericidal effects.
Article
Agriculture, Dairy & Animal Science
Dan Mu, Donggen Zhou, Guoyang Xie, Ju Liu, Zhengzheng Wang, Qin Xiong, Hengyi Xu
Summary: In this study, a method for detecting viable E. coli O157:H7 in food products was established using an enhanced nucleic acid binding dye called improved propidium monoazide (PMAxx) coupled with rRAA assay. The PMAxx-rRAA assay showed high sensitivity and good specificity, and could resist interference from dead bacteria and nontarget bacteria.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Food Science & Technology
Chuyan Chen, Claudia P. Coronel-Aguilera, Bruce M. Applegate, Andrew G. Gehring, Arun K. Bhunia, George C. Paoli
Summary: The USDA-FSIS has zero tolerance for E. coli O157:H7 in raw ground beef and currently collects samples from beef processing facilities for testing. This study assessed the potential of using a phage-based luminescence detection assay during sample transportation, and the results suggest that this approach is feasible.
Article
Food Science & Technology
Ziba Salimnejhad, Hassan Hassanzadazar, Majid Aminzare
Summary: This study aimed to evaluate the effects of Epinecidin-1 (Epi-1) on the total viable count, sensory properties, and survival of Escherichia Coli O157:H7 and Staphylococcus aureus in pasteurized milk samples during cold storage. The results showed that a concentration of 0.01% Epi-1 had the best antibacterial and antioxidant power. Milk samples containing 0.01% Epi-1 had the lowest total viable count and total psychrotrophic count at the end of the storage period, and a significant reduction of E. coli O157:H7 and S. aureus was observed. No unpleasant sensory properties were detected in the treated samples.
FOOD SCIENCE & NUTRITION
(2023)
Article
Chemistry, Multidisciplinary
Qiao He, Zhehao Yang, Zhipeng Zou, Mengyan Qian, Xiaolei Wang, Xinhui Zhang, Zhongping Yin, Jinhai Wang, Xingqian Ye, Donghong Liu, Mingming Guo
Summary: A new class of antimicrobial peptides (AMPs) called functionalized chickpea-derived Leg2 antimicrobial peptides (FCLAPs) is designed by modifying the structural parameters. Among the FCLAPs, KTA and KTR show superior antibacterial efficacy against Escherichia coli (E. coli) O157:H7 and demonstrate feasibility in alleviating E. coli O157:H7-induced intestinal infection. Combining omics profiling and peptide-membrane interaction assays, a unique dual-targeting mode of action is characterized.
Article
Biotechnology & Applied Microbiology
Ji Hyun Yoo, Ki Ho Baek, Ye Seul Heo, Hae In Yong, Cheorun Jo
Summary: The study demonstrated that the combined treatment of clove oil and encapsulated atmospheric pressure plasma (EAP) significantly enhanced bactericidal effects against Escherichia coli and Staphylococcus aureus, potentially by increasing bacterial susceptibility to EAP treatment. The synergistic effects were observed in both disc-diffusion assays and inoculated beef jerky samples.
Article
Microbiology
Yan Zhou, Qiyang Wan, Hongduo Bao, Yonghao Guo, Shujiao Zhu, Hui Zhang, Maoda Pang, Ran Wang
Summary: Phage SQ17 is a broad-host range lytic phage capable of infecting various E. coli strains, with a short latent period, high burst size, and stability. Experimental results demonstrate that SQ17 can effectively control the counts of EHEC O157:H7 and ETEC in milk, raw beef, and fresh lettuce.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biophysics
Yong Zhao, Yanzhao Li, Pingping Zhang, Ziheng Yan, Yugui Zhou, Yipu Du, Chunyu Qu, Yajun Song, Ding Zhou, Songnan Qu, Ruifu Yang
Summary: The study developed a highly sensitive fluorescent immunosensor using CDs-microspheres for the specific detection of Escherichia coli O157:H7 in milk, showing strong fluorescence intensity, good stability, and high uniformity in fluorescence. The immunosensor had a detection limit of 2.4 x 10(2) CFU/mL and could detect spiked E. coli O157:H7 in milk within 30 minutes with good repeatability. Additionally, the method could detect trace amounts of E. coli O157:H7 in real milk samples with 100% accuracy after bacterial enrichment, demonstrating its potential as a rapid and sensitive tool for pathogen detection in milk and other foods.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Agriculture, Dairy & Animal Science
Tao Yang, Zhenghui Wang, Yang Song, Xinyan Yang, Sihan Chen, Shiqian Fu, Xue Qin, Wei Zhang, Chaoxin Man, Yujun Jiang
Summary: A smartphone-based colorimetric aptasensor was developed for the detection of E. coli O157:H7 in milk, showing good reproducibility with no cross-reaction for other bacteria. The sensor could detect a concentration of 8.43 x 10^3 cfu/mL of E. coli O157:H7 in pure culture and 5.24 x 10^2 cfu/mL in artificially contaminated milk after 1 hour of incubation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Analytical
Zhihua Li, Xue Zhang, Haishan Qi, Xiaowei Huang, Jiyong Shi, Xiaobo Zou
Summary: A renewable electrochemical biosensor based on mussel-inspired adhesive protein was developed for the detection of Escherichia coli O157:H7. The biosensor showed excellent repeatability and sensitivity, with a wide linear range and a low detection limit. It demonstrated good performance in detecting E. coli O157:H7 in seafood samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Veterinary Sciences
Delphine Bibbal, Philippe Ruiz, Panagiotis Sapountzis, Christine Mazuy-Cruchaudet, Estelle Loukiadis, Frederic Auvray, Evelyne Forano, Hubert Brugere
Summary: This study aimed to identify farms with persistent circulation of EHEC O157:H7 in cattle. The results showed that persistent circulation may be due to the presence of a few predominant strains or repeated exposure of cattle to various strains. In addition, differences were found in the fecal microbial communities of shedders and non-shedders, with different bacterial indicators identified.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Nanoscience & Nanotechnology
Wei-Lun Huang, Chia-Jung Hsu, Jun Hao Wang, Zi-Han Hsu, Kuan-Hung Lu, Wen-Der Wang, Chia-Chi Huang
Summary: This study demonstrates that carbon nanodots can inhibit bacterial growth even without photoactivation, N-doping, and surface charge, showing potential applications in antibacterial wound dressing and food packaging. The nanodots exhibit antibacterial activities against various bacteria and manageable acute toxicity. The study reveals that the characteristics required for carbon nanodots to be antibacterial are not fully understood, and the biological pathways leading to such activities are more diverse than previously thought.
ACS APPLIED NANO MATERIALS
(2023)
Article
Engineering, Electrical & Electronic
Cuiyue Xu, Shengqi Cheng, Fan Zhang, Qingjiang Wang
Summary: A novel self-calibrated potentiometric sensors array based on peptide recognition has been proposed for simultaneous detection of Staphylococcus aureus (SA) and E. coli O157:H7 (E. coli). The array consists of a four-channel screen printed carbon electrode (SPCE) modified with dendrimer gold (DenAu) and polythiophene acetic acid (PTAA). The sensors array exhibits high sensitivity, good specificity and repeatability, and has been successfully applied to the detection of milk samples, providing an effective strategy for on-site simultaneous detection of multiple targets.
IEEE SENSORS JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Jingjing Zhang, Feng Qu, Taobo Liang, Sidi Lv, Wen Li, Qixiu You, Bolu Chen, Hengyi Xu
Summary: Synthesized Ag NPs coated with ε-polylysine and poly-ethyleneimine showed strong anti-P. aeruginosa capacity at low concentrations, inhibiting drug-resistant clinical strains and biofilm formation. The antibacterial mechanism was found to be primarily through induction of oxidative stress by increasing levels of ROS, which was neutralized by the ROS scavenger N-acetyl-L-cysteine.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Environmental Sciences
Huijuan Kuang, Wanyi Zhang, Lin Yang, Zoraida P. Aguilar, Hengyi Xu
Summary: This study investigated the effects of ZnO nanoparticles on the female and male reproductive organs in mice, finding that ZnO NPs caused more severe reproductive toxicity in male mice. This included decreased testes weight, imbalance of hematological and serum biochemical parameters, and cell apoptosis. The study suggests that ZnO NPs could pose exposure-related risks to reproductive health, especially in occupational settings.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Chemistry, Analytical
Xiaoyan Feng, Xiangyu Meng, Fangbin Xiao, Zoraida P. Aguilar, Hengyi Xu
Summary: A simple, rapid, highly sensitive, and specific method for detecting bacteria has been developed in this study, utilizing the Bio-den-Van multivalent capture nanoplatform in combination with m-qPCR for simultaneous detection of different bacteria with good detection limits. This method shows great potential for future clinical applications.
Article
Food Science & Technology
Yu Zhao, Yizhou Tang, Shanji Liu, Tiantian Jia, Donggen Zhou, Hengyi Xu
Summary: This study evaluated the stronger biological adverse effects of oral exposure to TiO2 NPs in a metabolic syndrome mouse model, finding that high-dose TiO2 NPs may induce liver inflammation, fibrosis, apoptosis, and worsen intestinal leakage in the model.
Article
Food Science & Technology
Ju Liu, Guoyang Xie, Qin Xiong, Taobo Liang, Hengyi Xu
Summary: A sensitive and specific assay was developed for detecting Cronobacter spp. in powdered infant formula samples, showing the potential for rapid and efficient detection. The method could detect bacteria at low concentrations after enrichment with high recovery rates in both pure culture and spiked samples.
Article
Agriculture, Multidisciplinary
Yu Zhao, Shanji Liu, Yizhou Tang, Tao You, Hengyi Xu
Summary: The study indicates that titanium dioxide nanoparticles may lead to diet-induced metabolic syndrome and cause inflammation damage in the liver and colon. Oral supplementation of Lactobacillus rhamnosus GG can alleviate inflammation and metabolic abnormalities, protecting against damage induced by nanoparticles.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Dairy & Animal Science
Shuang Yu, Qian Xu, Jin Huang, Bo Yi, Zoraida P. Aguilar, Hengyi Xu
Summary: This study presents a quick and reliable method for Salmonella detection based on the HCR-GO platform, with detection completed in a short time frame. The assay showed specificity and reliability in detecting Salmonella in different matrices.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Analytical
Yutong Wang, Zhengzheng Wang, Zhongxu Zhan, Ju Liu, Tingting Deng, Hengyi Xu
Summary: A strategy for detecting Staphylococcus aureus using magnetic separation technology combined with rolling circle amplification was proposed in this study, where antibiotics were used to capture bacteria and RCA products served as signal output probes, resulting in the enrichment and specific detection of S. aureus by combining magnetic separation and RCA technology.
ANALYTICA CHIMICA ACTA
(2022)
Article
Agriculture, Dairy & Animal Science
Dan Mu, Donggen Zhou, Guoyang Xie, Ju Liu, Zhengzheng Wang, Qin Xiong, Hengyi Xu
Summary: In this study, a method for detecting viable E. coli O157:H7 in food products was established using an enhanced nucleic acid binding dye called improved propidium monoazide (PMAxx) coupled with rRAA assay. The PMAxx-rRAA assay showed high sensitivity and good specificity, and could resist interference from dead bacteria and nontarget bacteria.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Food Science & Technology
Zhengzheng Wang, Ju Liu, Guanhua Chen, Xiaoyan Feng, Mei Deng, Dan Mu, Qian Xu, Hengyi Xu
Summary: A strategy for the detection of S. aureus using magnetic separation technology combined with colorimetry was proposed in this study. By pre-enriching S. aureus and constructing an untargeted magnetic separation based on APBA-MBs, the traditional targeted magnetic separation was successfully replaced. The detection limit of S. aureus under optimal experimental conditions was 3.0 x 10(2) CFU/mL.
Article
Agriculture, Dairy & Animal Science
Xiaoyan Feng, Donggen Zhou, Guoyang Xie, Ju Liu, Qin Xiong, Hengyi Xu
Summary: A rapid and specific method based on a fluorescence probe was developed for detecting viable K. pneumoniae in powdered infant formula samples. The method combined recombinase-aided amplification with a novel photosensitive DNA-intercalating dye, resulting in fast and accurate detection of K. pneumoniae within 40 minutes.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Hui Li, Yi Zhao, Jingjing Zhang, Wen Li, Qixiu You, Xianxiang Zeng, Hengyi Xu
Summary: This study investigated the effects of silver nanoparticles (AgNP) on the environmental tolerance and biofilm formation of Cronobacter sakazakii. The results showed that AgNP significantly reduced the survival rate of C. sakazakii under environmental stress conditions, inhibited biofilm formation, and downregulated the expression of genes related to stress tolerance. AgNP also inhibited ATP production and biofilm formation in C. sakazakii cells.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Xiaowei Xu, Shanji Liu, Yu Zhao, Mengqi Wang, Liehai Hu, Wenjuan Li, Hengyi Xu
Summary: Polysaccharides and probiotics can be combined to regulate gut microbiota and alleviate liver injury. The study found that the combination of Houttuynia cordata polysaccharide (HCP) and Lactiplantibacillus plantarum P101 can reduce oxidative stress and inflammatory injury in the liver by activating Nrf2 signals and inhibiting NF-kappa B signals. Furthermore, the combination increased the abundance of beneficial bacteria and reduced the pro-inflammatory bacteria in the gut microbiota. The findings suggest that polysaccharides combined with probiotics can regulate gut microbiota and alleviate liver injury.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Environmental Sciences
Siyue Wen, Yu Zhao, Shanji Liu, Hongbin Yuan, Tao You, Hengyi Xu
Summary: This study reveals the critical role of gut microbiota in microplastics-induced reproductive toxicity and attempts to elucidate the underlying mechanism of gut microbial dysregulation-mediated IL-17A signaling pathway.
ENVIRONMENTAL POLLUTION
(2022)