Cryo-EM Structures of SARS-CoV-2 Spike without and with ACE2 Reveal a pH-Dependent Switch to Mediate Endosomal Positioning of Receptor-Binding Domains

A pneumonia outbreak associated with a new coronavirus of probable bat origin

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Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation

(2020) Daniel Wrapp et al.   SCIENCE

Structural and Functional Basis of SARS-CoV-2 Entry by Using Human ACE2

(2020) Qihui Wang et al.   CELL

Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein

(2020) Alexandra C. Walls et al.   CELL

SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor

(2020) Markus Hoffmann et al.   CELL

Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor

(2020) Jun Lan et al.   NATURE

Structural basis of receptor recognition by SARS-CoV-2

(2020) Jian Shang et al.   NATURE

A highly conserved cryptic epitope in the receptor binding domains of SARS-CoV-2 and SARS-CoV

(2020) Meng Yuan et al.   SCIENCE

Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV

(2020) Xiuyuan Ou et al.   Nature Communications

Structure of mouse coronavirus spike protein complexed with receptor reveals mechanism for viral entry

(2020) Jian Shang et al.   PLoS Pathogens

The Impact of Mutations in SARS-CoV-2 Spike on Viral Infectivity and Antigenicity

(2020) Qianqian Li et al.   CELL

Tracking Changes in SARS-CoV-2 Spike: Evidence that D614G Increases Infectivity of the COVID-19 Virus

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Neutralization of SARS-CoV-2 by Destruction of the Prefusion Spike

(2020) Jiandong Huo et al.   Cell Host & Microbe

Convergent antibody responses to SARS-CoV-2 in convalescent individuals

(2020) Davide F. Robbiani et al.   NATURE

Potent neutralizing antibodies against multiple epitopes on SARS-CoV-2 spike

(2020) Lihong Liu et al.   NATURE

SARS-CoV-2 and bat RaTG13 spike glycoprotein structures inform on virus evolution and furin-cleavage effects

(2020) Antoni G. Wrobel et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model

(2020) Thomas F. Rogers et al.   SCIENCE

Distinct conformational states of SARS-CoV-2 spike protein

(2020) Yongfei Cai et al.   SCIENCE

Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability

(2020) Philip J. M. Brouwer et al.   SCIENCE

Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant

(2020) Leonid Yurkovetskiy et al.   CELL

Receptor binding and priming of the spike protein of SARS-CoV-2 for membrane fusion

(2020) Donald J. Benton et al.   NATURE

SARS-CoV-2 neutralizing antibody structures inform therapeutic strategies

(2020) Christopher O. Barnes et al.   NATURE

Structures and distributions of SARS-CoV-2 spike proteins on intact virions

(2020) Zunlong Ke et al.   NATURE

Spike mutation D614G alters SARS-CoV-2 fitness

(2020) Jessica A. Plante et al.   NATURE

In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges

(2020) Beata Turoňová et al.   SCIENCE

Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes

(2020) Tongqing Zhou et al.   Cell Reports

Real-Time Conformational Dynamics of SARS-CoV-2 Spikes on Virus Particles

(2020) Maolin Lu et al.   Cell Host & Microbe

Positive-unlabeled convolutional neural networks for particle picking in cryo-electron micrographs

(2019) Tristan Bepler et al.   NATURE METHODS

Cryo-EM structure of infectious bronchitis coronavirus spike protein reveals structural and functional evolution of coronavirus spike proteins

(2018) Jian Shang et al.   PLoS Pathogens

Automated map sharpening by maximization of detail and connectivity

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Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2

(2018) Wenfei Song et al.   PLoS Pathogens

New tools for the analysis and validation of cryo-EM maps and atomic models

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MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen

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UCSF ChimeraX: Meeting modern challenges in visualization and analysis

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Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains

(2017) Yuan Yuan et al.   Nature Communications

Cryo-electron microscopy structures of the SARS-CoV spike glycoprotein reveal a prerequisite conformational state for receptor binding

(2016) Miao Gui et al.   CELL RESEARCH

Pre-fusion structure of a human coronavirus spike protein

(2016) Robert N. Kirchdoerfer et al.   NATURE

Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy

(2016) Alexandra C Walls et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy

(2016) Alexandra C Walls et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

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Quantifying the local resolution of cryo-EM density maps

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RELION: Implementation of a Bayesian approach to cryo-EM structure determination

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Outcome of the First Electron Microscopy Validation Task Force Meeting

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Evaluating Nanoparticle Sensor Design for Intracellular pH Measurements

(2011) Rikke V. Benjaminsen et al.   ACS Nano

Improved Treatment of Ligands and Coupling Effects in Empirical Calculation and Rationalization of pKaValues

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PROPKA3: Consistent Treatment of Internal and Surface Residues in Empirical pKaPredictions

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Focused Evolution of HIV-1 Neutralizing Antibodies Revealed by Structures and Deep Sequencing

(2011) X. Wu et al.   SCIENCE

PHENIX: a comprehensive Python-based system for macromolecular structure solution

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Lysosomes as “Suicide Bags” in Cell Death: Myth or Reality?

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