Article
Engineering, Manufacturing
Frederik Wulle, Oliver Gorke, Sarah Schmidt, Maximilian Nistler, Gunter E. Tover, Oliver Riedel, Alexanser Verl, Achim Weber, Alexanser Southan
Summary: This paper introduces the potential and advantages of multi-axis 3D printing of hydrogels, and demonstrates through experiments the relative flexibility and water absorbency of 3D printed hydrogels compared to conventional molded hydrogels. The research also highlights related research questions, such as cross-linking chemistry and formulation of hydrogel inks.
ADDITIVE MANUFACTURING
(2022)
Review
Biotechnology & Applied Microbiology
Earnest P. Chen, Zeren Toksoy, Bruce A. Davis, John P. Geibel
Summary: The aim of tissue engineering with 3D bioprinting is to construct fully functional tissue and organ replacements for clinical applications. One of the main challenges is maintaining tissue viability through the inclusion of complex vascular networks. Recent advancements have made great strides in incorporating vascular networks in 3D printed tissue and organs, offering new possibilities for creating biologically functional tissues and organs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Engineering, Environmental
Arkodip Mandal, Kaushik Chatterjee
Summary: 4D bioprinting using water as a stimulus shows great potential in tissue engineering by addressing the challenges of smart hydrogels and cytocompatible stimuli.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Engineering, Multidisciplinary
Zizhuo Zheng, David Eglin, Mauro Alini, Geoff R. Richards, Ling Qin, Yuxiao Lai
Summary: Three-dimensional (3D) bioprinting is an emerging technology that assembles biocompatible materials and cells to create tissue engineering solutions. Photopolymerized materials play a crucial role in the success of 3D bioprinting. Visible light is considered more biocompatible than UV light for bioprinting applications.
Review
Biochemistry & Molecular Biology
Sven Pantermehl, Steffen Emmert, Aenne Foth, Niels Grabow, Said Alkildani, Rainer Bader, Mike Barbeck, Ole Jung
Summary: The use of additive manufacturing technology in modern medicine is a relatively new field, offering fast and effective ways to produce personalized implants, tissues, and organs for patients. This review provides an overview of various 3D printing technologies, discussing their functions, strengths, and weaknesses in medical applications, as well as summarizing previous studies in tissue engineering.
Review
Polymer Science
Jianghong Huang, Jianyi Xiong, Daping Wang, Jun Zhang, Lei Yang, Shuqing Sun, Yujie Liang
Summary: 3D bioprinting is an emerging technology that allows for personalized therapy strategies in regenerative medicine. Over the last two decades, 3D bioprinting hydrogels have significantly advanced the field of cartilage and bone tissue engineering. This review discusses the development and application of 3D bioprinting, as well as future directions for optimization.
Article
Polymer Science
Alessio Bucciarelli, Mauro Petretta, Brunella Grigolo, Laura Gambari, Alessandra Maria Bossi, Francesco Grassi, Devid Maniglio
Summary: In this study, a crosslinked methacrylated silk (Sil-MA) grid structure was developed, which can recover its shape under UV light and has biocompatibility and osteogenic support. Sil-MA was chemically and physically characterized, and tested for its biocompatibility according to ISO protocols. Digital Light Processing and pneumatic extrusion techniques were used to manufacture the Sil-MA grid structure, demonstrating its shape memory property and potential application in bone regeneration.
Review
Engineering, Biomedical
Reza Noroozi, Zia Ullah Arif, Hadi Taghvaei, Muhammad Yasir Khalid, Hossein Sahbafar, Amin Hadi, Ali Sadeghianmaryan, Xiongbiao Chen
Summary: Bioprinting is a revolutionary technology in additive manufacturing that prints 3D cell-laden constructs for various clinical applications. It uses biomaterials and cells to regenerate tissues like heart, bone, cornea, cartilage, nerve, and skin, and also contributes to drug delivery, wound healing, bio-actuators, bio-robotics, and bio-sensors. The development of 4D bioprinting and stimuli-responsive materials has further enhanced its potential and led to a billion-dollar market. This study reviews the concepts, applications, and future research topics of 3D and 4D bioprinting, emphasizing the need for collaboration among clinicians, engineers, scientists, and regulatory bodies to translate this technology into biomedical systems.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Senthilkumar Muthusamy, Sathya Kannan, Marcus Lee, Vijayavenkataraman Sanjairaj, Wen Feng Lu, Jerry Y. H. Fuh, Gopu Sriram, Tong Cao
Summary: Bioprinting technology has made significant progress over the past decade, particularly in developing larger and more physiologic tissues. By precisely combining biological scaffold materials and cells in three-dimensional space, bioprinting has the potential to assist in locating endothelial cells in specific spatial positions to promote vessel formation in predefined areas.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Review
Biochemistry & Molecular Biology
Gabriel Alexander Salg, Andreas Blaeser, Jamina Sofie Gerhardus, Thilo Hackert, Hannes Goetz Kenngott
Summary: Among advanced therapy medicinal products, tissue-engineered products have the potential to address the current shortage of donor organs and provide alternative options in organ replacement therapy. One of the main challenges in developing bioartificial parenchymal tissue is the establishment of a sufficient blood vessel network. Three-dimensional bioprinting, through precise bioink deposition, has the potential to overcome this challenge. Bioinks can contain cells, matrix compounds, and signaling molecules to promote vascularization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Stefan Jentsch, Ramin Nasehi, Christoph Kuckelkorn, Benedikt Gundert, Sanja Aveic, Horst Fischer
Summary: Bioprinting faces challenges such as nozzle limitations and high shear stress, which can lead to cell damage. A novel 3D bioprinting method based on acoustic droplet ejection (ADE) is introduced to minimize critical shear stress and enable precise printing of cell-laden structures. This multiscale acoustic bioprinting technique shows promise for creating complex and individualized 3D hydrogel structures while preserving cell viability.
Review
Polymer Science
Alireza Ghofrani, Lozaalsadat Taghavi, Bahareh Khalilivavdareh, Anahita Rohani Shirvan, Alireza Nouri
Summary: Cardiac patches made of polymer scaffolds and heart muscle cells have the potential to repair damaged heart tissue and improve its function. Traditional methods have limitations in controlling the structure and reproducibility of the patches, while 3D bioprinting technology allows for flexible design and precise placement based on individual patient needs. Recent research has focused on enhancing various functionalities of cardiac patches and addressing the challenge of adhesion to heart tissue.
EUROPEAN POLYMER JOURNAL
(2022)
Review
Biochemistry & Molecular Biology
Jia Min Lee, Sean Kang Qiang Suen, Wei Long Ng, Wai Cheung Ma, Wai Yee Yeong
Summary: This study aims to provide a comprehensive overview of collagen printing, understanding collagen assembly in vivo and various sources of collagen available for tissue engineering applications. It also highlights current printing technologies and strategies for collagen-based materials, identifies considerations and key challenges faced in collagen printing, and presents key research areas to enhance the functionality of printed collagen.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Biochemical Research Methods
Sara Simorgh, Neda Alasvand, Mahboobe Khodadadi, Faezeh Ghobadi, Maziar Malekzadeh Kebria, Peiman Brouki Milan, Saeid Kargozar, Francesco Baino, Ali Mobasheri, Masoud Mozafari
Summary: Tissue engineering and regenerative medicine use additive manufacturing technology for 3D printing, with bioactive glasses being one of the most promising raw materials that can improve the bioactivity and antibacterial activity of constructs. This review provides an up-to-date report on the development of 3D printed scaffolds using BG-containing raw biomaterials for tissue regeneration applications, focusing on their advantages and challenges.
Review
Engineering, Biomedical
Silvia Santoni, Simone G. Gugliandolo, Mattia Sponchioni, Davide Moscatelli, Bianca M. Colosimo
Summary: Bioprinting is a multidisciplinary research field that combines additive manufacturing, biology, and material sciences to create bioconstructs resembling natural living tissues. This field has gained significant interest due to the possibility of reproducing biological tissues and organs, and has attracted extensive research efforts from companies, universities, and research institutes. This paper reviews and analyzes the scientific literature and patenting results from 2000-2020 to provide an overview of the scientific and industrial landscape of bioprinting.
BIO-DESIGN AND MANUFACTURING
(2022)
Review
Engineering, Biomedical
Berivan Cecen, Ayca Bal-Ozturk, Gokcen Yasayan, Emine Alarcin, Polen Kocak, Rumeysa Tutar, Leyla Didem Kozaci, Su Ryon Shin, Amir K. Miri
Summary: This article reviews the natural-based biomaterials used in micro-tissue models of organ-on-a-chip devices, and discusses their characteristics, preparation methods, modification methods, and characterization methods. The article also summarizes recent reports on the applications of these biomaterials in the design and fabrication of micro-tissue models. This information will be helpful for bioengineers in selecting the appropriate biomaterials to meet clinical expectations and improve patient outcomes.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Engineering, Biomedical
Anant Bhusal, Elvan Dogan, Hai-Anh Nguyen, Olga Labutina, Daniel Nieto, Ali Khademhosseini, Amir K. Miri
Summary: This study developed a multi-material DLP-based bioprinter for rapid prototyping of hydrogel-based microfluidic chips. The optimized composite hydrogel bioink allows for a wide range of mechanical properties. The biofabrication approach offers a useful tool for integrating micro-tissue models into organs-on-chips and high-throughput drug screening platforms.
Review
Biotechnology & Applied Microbiology
Amirhossein Moghanian, Berivan Cecen, Nazanin Nafisi, Zahra Miri, Derek H. Rosenzweig, Amir K. Miri
Summary: Recent dental repair strategies involve the use of various bioactive materials, such as synthetic polymers, natural polymers, bioceramics, and self-assembling peptides. These materials promote tissue growth and vascularization in the dental complex. This review summarizes the advantages and challenges of current dental biomaterials, and discusses the application of modern manufacturing techniques in delivering biomaterials into the dental complex.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Gizem Baysan, Oylum Colpankan Gunes, Pinar Akokay, R. Bugra Husemoglu, Pinar Ertugruloglu, Aylin Ziylan Albayrak, Berivan Cecen, Hasan Havitcioglu
Summary: This study aimed to develop a new type of meniscus scaffold made of chitosan, loofah mat, and PHBV nanofibers and evaluate its properties. The results showed that the scaffold supported cell proliferation and chondrogenesis, making it a promising material for engineering meniscus tissue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Materials Science, Multidisciplinary
Hossein Goodarzi Hosseinabadi, Daniel Nieto, Ali Yousefinejad, Hoda Fattel, Leonid Ionov, Amir K. Miri
Summary: Digital light processing (DLP) 3D printing is a powerful tool for manufacturing complex structures quickly, with research on optical design and ink selection being key to its rapid progress. This critical review addresses the challenges in printing photopolymerizable inks using DLP technology, solving the kinetics equations of the photopolymerization reaction and explaining the influence of optical parameters and ink chemical properties on curing depth. It summarizes developments in DLP platform design and ink selection, and presents experimental data showing the impact of monomer structure and molecular weight on printing resolution. A detailed guideline is provided for selecting inks and optical parameters for multi-material and 4D printing of functional structures.
APPLIED MATERIALS TODAY
(2023)
Article
Biophysics
Berivan Cecen, Ecem Saygili, Iman Zare, Omid Nejati, Danial Khorsandi, Atefeh Zarepour, Emine Alarcin, Ali Zarrabi, Seda Nur Topkaya, Ozlem Yesil-Celiktas, Ebrahim Mostafavi, Ayca Bal-Ozturk
Summary: Developing effective treatments for neurological disorders is extremely challenging due to the complexity of the brain. However, advances in in vitro systems, particularly the brain-on-a-chip (BoC), have allowed researchers to simulate critical components of the brain's structure and function. This review provides an overview of the practical concerns and potential applications of BoC systems, including integrating biosensing technologies, automating assays, and addressing key challenges for clinical translation.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Berivan Cecen
Summary: This study explores the design and replication methods for polylactide (PLA)/polyhydroxyalkanoate (PHA) scaffolds with a specific porosity using 3D printing-fused deposition modeling technique. The mechanical properties of triple periodic minimal surfaces (TPMS)-based lattices were examined, along with the effects of different lattice types and elastic modulus on the materials. PLA/PHA scaffolds are widely used for bone fixation due to their enhanced mechanical performance. 3D printing technologies allow for enhanced pore size controls and the creation of complex architectural scaffolds with biofunctions.
Review
Chemistry, Medicinal
Ayca Bal-Ozturk, Ece Ozcan-Bulbul, Hazal Ezgi Gultekin, Berivan Cecen, Ebru Demir, Atefeh Zarepour, Sibel Cetinel, Ali Zarrabi
Summary: Eyes are crucial for providing the brain with information about the environment. Ocular diseases can severely impact quality of life and finding effective treatment methods has become a priority. Recent innovations in contact lenses, micro and nanoneedles, and in situ gels have shown promise in overcoming barriers in delivering therapeutic drugs into the eyes. This review aims to summarize the evidence on the effectiveness of these new techniques, their progression in preclinical and clinical studies, current limitations, and future prospects.
Article
Engineering, Biomedical
Shabir Hassan, Ting Wang, Kun Shi, Yike Huang, Maria Elizabeth Urbina Lopez, Kaifeng Gan, Mo Chen, Niels Willemen, Haroon Kalam, Eder Luna-Ceron, Berivan Cecen, Gihan Daw Elbait, Jinghang Li, Luis Enrique Garcia-Rivera, Melvin Gurian, Mudassir Meraj Banday, Kisuk Yang, Myung Chul Lee, Weida Zhuang, Castro Johnbosco, Oju Jeon, Eben Alsberg, Jeroen Leijten, Su Ryon Shin
Summary: Oxygenating biomaterials have been found to have beneficial effects on tissue formation, but the impact of oxygen-generating materials on tissue formation is not well understood. This study investigates the effects of calcium peroxide-based oxygen-generating microparticles on the osteogenic fate of human mesenchymal stem cells (hMSCs) under severely oxygen-deficient conditions. The results show that hydrogels containing oxygen-generating microparticles improve osteogenic differentiation of hMSCs under both normal and oxygen-deficient conditions. The study also demonstrates that hydrogels with oxygen-generating microparticles can induce the formation of functional engineered tissues, holding potential for biomedical applications such as tissue regeneration and organ replacement therapy.
Review
Materials Science, Biomaterials
Nagavendra Kommineni, Ruchita Chaudhari, Joao Conde, Sedef Tamburaci, Berivan Cecen, Pranjal Chandra, Rajendra Prasad
Summary: Engineered liposomal nanoparticles have unique characteristics and have shown significant progress as cargo carriers in cancer care and therapeutics. Liposomal theranostics have been approved by the FDA and reached the market. Nanosized liposomes are proven systems for delivering therapeutics and imaging agents to target sites, but the integration of imaging and therapeutic probes within liposomes may compromise their overall performance.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Green & Sustainable Science & Technology
E. T. Aksun Tuemerkan, L. D. Kozaci, A. K. Miri, S. Maharjan, B. Cecen
Summary: The disposal of aquatic waste requires modifying it with sterile, non-hazardous, and ecological procedures, which is a significant challenge. Biomaterials from aquatic species and their waste or by-products, such as plants, fish, waste biomass, biopolymers, and stabilization agents, are considered renewable biosources. These biomaterials can be used in biomedicine, pharmacology, and tissue engineering. This review provides an overview of the different groups of aquatic biomaterials and their potential applications, helping bioengineers find more economical and eco-compatible options.
MATERIALS TODAY SUSTAINABILITY
(2023)
Article
Materials Science, Multidisciplinary
Gizem Baysan, Oylum Colpankan Gunes, Cihangir Turemis, Pinar Akokay Yilmaz, R. Bugra Husemoglu, Aylin Kara Ozenler, Merve Perpelek, Aylin Ziylan Albayrak, Hasan Havitcioglu, Berivan Cecen
Summary: This article reports on a new scaffold design for a loofah-reinforced chitosan-collagen hydrogel composite scaffold. The scaffold, crosslinked with 0.5% genipin, showed suitable properties for further in vitro and in vivo studies. In vitro analysis demonstrated the scaffold's support for rabbit mesenchymal stem cells (rMSCs) adhesion and proliferation. In vivo cartilage healing studies with cell-laden scaffolds showed better osteochondral healing.
Article
Nanoscience & Nanotechnology
Anant Bhusal, Elvan Dogan, Daniel Nieto, Seyed Ali Mousavi Shaegh, Berivan Cecen, Amir K. Miri
Summary: This study presents a manufacturing approach for bioprinted microfluidic chips, which can address the limitations of conventional high-throughput screening platforms. The chips exhibit predefined gradients of biochemical properties and are capable of generating drug/agent gradients.
ACS APPLIED BIO MATERIALS
(2022)
