Article
Biochemistry & Molecular Biology
Chun-Hsiung Wang, Dong-Hua Chen, Shih-Hsin Huang, Yi-Min Wu, Yi-Yun Chen, Yeukuang Hwu, David Bushnell, Roger Kornberg, Wei-Hau Chang
Summary: The study explored the ability of using multipurpose microscopes to determine the structures of infectious particles, revealing that resolution is ultimately limited by the depth of field, while high-end cryo-EMs can achieve virtually the same resolution when determining similar virus structures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Dimitry Tegunov, Liang Xue, Christian Dienemann, Patrick Cramer, Julia Mahamid
Summary: The software tool M establishes a reference-based multi-particle refinement framework for cryo-EM data and enables residue-level structure determination inside cells when combined with CTF correction and map denoising.
Review
Biochemistry & Molecular Biology
Nan Liu, Hong-Wei Wang
Summary: Cryogenic electron microscopy (cryo-EM) is a powerful and widely used method for determining high-resolution structures of macromolecules. The main challenge lies in preparing high-quality vitrified specimens, which face various practical difficulties. This review discusses the problems caused by the air-water interface (AWI) and proposes strategies to overcome them, including the use of additives, fast vitrification devices, and thin layer supporting films. These approaches have been proven successful in cryo-EM specimen preparation.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Physical
Radim Skoupy, Daan B. B. Boltje, Miroslav Slouf, Katerina Mrazova, Tomas Laznicka, Clemence M. Taisne, Vladislav Krzyzanek, Jacob P. P. Hoogenboom, Arjen J. J. Jakobi
Summary: This study presents a quantitative four-dimensional scanning transmission electron microscopy (4D-STEM) imaging technique (q4STEM) for local thickness estimation across amorphous specimens. The method measures spatially resolved diffraction patterns and evaluates the angular distribution of electron scattering or the ratio of integrated virtual dark and bright field STEM signals using Monte Carlo simulations. The method is independent of signal intensity calibrations and only requires knowledge of the detector geometry. The results demonstrate that the method yields robust thickness estimates for sub-micrometer amorphous specimens in different detectors and instruments.
Article
Multidisciplinary Sciences
Alastair T. Gardiner, Katerina Naydenova, Pablo Castro-Hartmann, Tu C. Nguyen-Phan, Christopher J. Russo, Kasim Sader, C. Neil Hunter, Richard J. Cogdell, Pu Qian
Summary: The study reports the structure of the LH2 complex from Marichromatium purpuratum with a resolution of 2.4 angstroms determined by cryogenic electron microscopy. It reveals a heptameric ring unique among all known LH2 structures, controlling the resonant coupling of the long-wavelength energy absorption band, and describes distinct carotenoids and energy transfer pathways within the complex. These details provide key insights into the assembly and oligomeric form of purple bacterial LH2 complexes that were previously inaccessible by any technique.
Article
Biochemical Research Methods
Di Liu, Francois A. Thelot, Joseph A. Piccirilli, Maofu Liao, Peng Yin
Summary: This study presents a nanoarchitectural engineering strategy, called ROCK, which enables efficient structural determination of RNA using cryo-EM. By installing kissing-loop sequences onto RNAs, ROCK allows for better self-assembly and higher resolution reconstruction. The application of ROCK to different RNA structures demonstrates its potential in enabling high-quality cryo-EM studies.
Article
Chemistry, Multidisciplinary
Jan-Philip Wieferig, Deryck J. Mills, Werner Kuehlbrandt
Summary: Cryo-EM technology is facing challenges in high-resolution structure determination of biological specimens due to beam-induced movement. Controlled devitrification of frozen samples reduces movement and enhances structure quality, contributing significantly to achieving high-resolution structure determination.
Article
Biology
Fumiya K. Sano, Hiroaki Akasaka, Wataru Shihoya, Osamu Nureki, David Drew
Summary: This study reports the structure of the complex between the endothelin ETB receptor and G-protein, revealing how endothelin activates the ETB receptor and expanding the diversity of G-protein binding modes.
Article
Cell Biology
Linhua Tai, Yun Zhu, He Ren, Xiaojun Huang, Chuanmao Zhang, Fei Sun
Summary: The nuclear pore complex (NPC) is a large protein complex in eukaryotes that regulates the transport of molecules between the nucleus and cytoplasm. By using cryo-electron microscopy and AlphaFold2, researchers determined the structure of the outer rings of the Xenopus laevis NPC and identified the composition and interactions of its components. This detailed structural information represents a significant advancement in understanding the assembly of the NPC.
Article
Biochemistry & Molecular Biology
Wim J. H. Hagen
Summary: Researchers have presented an energy filter-based plasmon imaging method for rapid screening of gold foil grids, in order to save expensive imaging time.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Virology
Alessio d'Acapito, Thomas Roret, Eleftherios Zarkadas, Pierre-Yves Mocaer, Florian Lelchat, Anne-Claire Baudoux, Guy Schoehn, Emmanuelle Neumann
Summary: Most studied bacteriophages are terrestrial viruses, but marine phages play a crucial role in oceanic regulation. However, they are often overlooked by the scientific community. By lysing bacterial population daily, marine phages have a significant impact on bacterial biomass, evolution, and global biogeochemical cycles.
JOURNAL OF VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Bing Rao, Shaobai Li, Deqiang Yao, Qian Wang, Ying Xia, Yi Jia, Yafeng Shen, Yu Cao
Summary: The study demonstrates that human Derlin-1 forms a protein channel for translocation of misfolded proteins, consisting of a large tunnel traversing the ER membrane and a lateral gate within the membrane. This structure differs from the previously reported monomeric yeast Derlin structure, which forms a semi-channel.
Article
Biochemistry & Molecular Biology
Huaxin Yu, Tasuku Hamaguchi, Yoshiki Nakajima, Koji Kato, Keisuke Kawakami, Fusamichi Akita, Koji Yonekura, Jian-Ren Shen
Summary: Photosystem II (PSII) functions mainly as a dimer to catalyze the light energy conversion and water oxidation reactions, but monomeric PSII also exists and functions in vivo. The structure of PSII monomer has been solved at a resolution of 2.78 angstrom using cryo-electron microscopy (cryo-EM), revealing differences in pigments and lipids between the monomer and dimer interfaces, highlighting the importance of beta-carotene, SQDG, and PsbO in the formation of PSII dimer.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Biochemical Research Methods
Grant Zane, Mark Silveria, Nancy Meyer, Tommi White, Rui Duan, Xiaoqin Zou, Michael Chapman
Summary: This study presents a high-resolution cryo-electron microscopy structure (2.2 angstrom) of AAV4, showing significant differences in variable regions I, III, IV, and V compared to AAV2, explaining its inability to bind with PKD2. Furthermore, differences between AAV4 and AAV5 in variable region VII are shown to be sufficient to exclude binding with PKD1.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tae Jin Jeon, Seong-Gyu Lee, Suk Hyun Yoo, Myeongbin Kim, Dabin Song, Joonghyun Ryu, Hwangseo Park, Deok-Soo Kim, Jaekyung Hyun, Ho Min Kim, Seong Eon Ryu
Summary: In this study, cryoelectron microscopy was used to investigate bovine respiratory supercomplexes, revealing large intercomplex motions and a dynamic substrate pool between complexes I and III. These findings provide insights into the regulation of metabolic flux and reactive oxygen species by supercomplexes.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Christopher J. Russo, Lori A. Passmore
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Christopher J. Russo, Lori A. Passmore
JOURNAL OF STRUCTURAL BIOLOGY
(2016)
Article
Multidisciplinary Sciences
Katerina Naydenova, Christopher J. Russo
NATURE COMMUNICATIONS
(2017)
Article
Microscopy
Christopher J. Russo, Richard Henderson
Article
Microscopy
Christopher J. Russo, Richard Henderson
Article
Microscopy
Christopher J. Russo, Richard Henderson
Article
Microscopy
Mathew J. Peet, Richard Henderson, Christopher J. Russo
Article
Multidisciplinary Sciences
Katerina Naydenova, Mathew J. Peet, Christopher J. Russo
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Multidisciplinary Sciences
Alastair T. Gardiner, Katerina Naydenova, Pablo Castro-Hartmann, Tu C. Nguyen-Phan, Christopher J. Russo, Kasim Sader, C. Neil Hunter, Richard J. Cogdell, Pu Qian
Summary: The study reports the structure of the LH2 complex from Marichromatium purpuratum with a resolution of 2.4 angstroms determined by cryogenic electron microscopy. It reveals a heptameric ring unique among all known LH2 structures, controlling the resonant coupling of the long-wavelength energy absorption band, and describes distinct carotenoids and energy transfer pathways within the complex. These details provide key insights into the assembly and oligomeric form of purple bacterial LH2 complexes that were previously inaccessible by any technique.
Article
Microscopy
Katerina Naydenova, Christopher J. Russo
Summary: The manufacturing process aims to create supports for cryoEM specimens with nanometre scale features and micron scale support structure, facilitating the location and identification of specific regions at various length scales.
Article
Microscopy
Joshua L. Dickerson, Peng-Han Lu, Dilyan Hristov, Rafal E. Dunin-Borkowski, Christopher J. Russo
Summary: This study investigates the potential improvements in using electron cryomicroscopy to image thick specimens with high-resolution phase contrast imaging. The research demonstrates that chromatic aberration correction can enhance the imaging of thick biological samples. The study also suggests that with advancements in transmission electron microscope technology, it may be possible to directly locate and identify sub-100 kDa proteins in vitrified specimens taken from cells without the need for labels.
Article
Microscopy
Joshua L. Dickerson, Christopher J. Russo
Summary: The study found that the phase-contrast signal of particles at different positions along the beam axis in a thick specimen is the same, contradicting depth-dependent phase contrast models in the literature.
Article
Microscopy
Katerina Naydenova, Akiko Kamegawa, Mathew J. Peet, Richard Henderson, Yoshinori Fujiyoshi, Christopher J. Russo
Summary: The fading of electron diffraction spots from 2D crystals of paraffin, purple membrane, and aquaporin 4 at different temperatures were studied. Results showed that the spots at resolutions between 3 Å and 20 Å fade slower at liquid helium temperatures compared to liquid nitrogen temperatures. This finding suggests that reducing the temperature of specimens during irradiation could improve electron cryomicroscopy of biological specimens.
Article
Chemistry, Physical
Christopher J. Russo, Joshua L. Dickerson, Katerina Naydenova
Summary: In the past decade, electron cryomicroscopy (cryoEM) has made significant progress and continues to advance rapidly. Within the next decade, there is the potential to determine atomic structures for thousands of protein and nucleic acid molecular complexes. Precisely identifying and quantifying the errors of these structures within cells could provide answers to many important biological questions.
FARADAY DISCUSSIONS
(2022)