Pyruvate-responsive genetic circuits for dynamic control of central metabolism

Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis

(2019) Wenlong Ma et al.   Microbial Cell Factories

Systems Metabolic Engineering Strategies: Integrating Systems and Synthetic Biology with Metabolic Engineering

(2019) Kyeong Rok Choi et al.   TRENDS IN BIOTECHNOLOGY

Developing a pyruvate-driven metabolic scenario for growth-coupled microbial production

(2019) Jian Wang et al.   METABOLIC ENGINEERING

Metabolic engineering of carbon overflow metabolism of Bacillus subtilis for improved N-acetyl-glucosamine production

(2018) Wenlong Ma et al.   BIORESOURCE TECHNOLOGY

Development of N -acetylneuraminic acid responsive biosensors based on the transcriptional regulator NanR

(2018) Gert Peters et al.   BIOTECHNOLOGY AND BIOENGINEERING

Advances and prospects of Bacillus subtilis cellular factories: From rational design to industrial applications

(2018) Yang Gu et al.   METABOLIC ENGINEERING

Optogenetic regulation of engineered cellular metabolism for microbial chemical production

(2018) Evan M. Zhao et al.   NATURE

Corrigendum: Progress in and promise of bacterial quorum sensing research

(2018) Marvin Whiteley et al.   NATURE

Layered dynamic regulation for improving metabolic pathway productivity inEscherichia coli

(2018) Stephanie J. Doong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis

(2018) Dingyu Liu et al.   ACS Synthetic Biology

Synthetic Repetitive Extragenic Palindromic (REP) sequence as an efficient mRNA stabilizer for protein production and metabolic engineering in prokaryotic cells

(2018) Chen Deng et al.   BIOTECHNOLOGY AND BIOENGINEERING

One-pot two-strain system based on glucaric acid biosensor for rapid screening of myo-inositol oxygenase mutations and glucaric acid production in recombinant cells

(2018) Shuang Zheng et al.   METABOLIC ENGINEERING

Synthetic redesign of central carbon and redox metabolism for high yield production of N-acetylglucosamine in Bacillus subtilis

(2018) Yang Gu et al.   METABOLIC ENGINEERING

Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors

(2018) Adam J. Meyer et al.   Nature Chemical Biology

Regulatory non-coding sRNAs in bacterial metabolic pathway engineering

(2018) Abigail N. Leistra et al.   METABOLIC ENGINEERING

Assessing glycolytic flux alterations resulting from genetic perturbations in E. coli using a biosensor

(2017) Christina E. Lehning et al.   METABOLIC ENGINEERING

Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit

(2017) Apoorv Gupta et al.   NATURE BIOTECHNOLOGY

A Glucose-Sensing Toggle Switch for Autonomous, High Productivity Genetic Control

(2017) William Bothfeld et al.   ACS Synthetic Biology

Antisense transcription as a tool to tune gene expression

(2016) J. A. Brophy et al.   Molecular Systems Biology

Arginine phosphorylation marks proteins for degradation by a Clp protease

(2016) Débora Broch Trentini et al.   NATURE

The role of repressor kinetics in relief of transcriptional interference between convergent promoters

(2016) Nan Hao et al.   NUCLEIC ACIDS RESEARCH

Stop that Noise and Turn Up the Antisense Transcription

(2016) Jan Steensels et al.   Cell Reports

Engineering an allosteric transcription factor to respond to new ligands

(2015) Noah D Taylor et al.   NATURE METHODS

Auto-inducible expression system based on the SigB-dependent ohrB promoter in Bacillus subtilis

(2014) R. Panahi et al.   MOLECULAR BIOLOGY

Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for Pyruvate

(2014) Alejandro San Martín et al.   PLoS One

Improving fatty acids production by engineering dynamic pathway regulation and metabolic control

(2014) P. Xu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Design and Kinetic Analysis of a Hybrid Promoter–Regulator System for Malonyl-CoA Sensing in Escherichia coli

(2013) Peng Xu et al.   ACS Chemical Biology

Computational design of ligand-binding proteins with high affinity and selectivity

(2013) Christine E. Tinberg et al.   NATURE

Gene regulation by antisense transcription

(2013) Vicent Pelechano et al.   NATURE REVIEWS GENETICS

Transcription Activation by NtcA in the Absence of Consensus NtcA-Binding Sites in an Anabaena Heterocyst Differentiation Gene Promoter

(2012) S. Camargo et al.   JOURNAL OF BACTERIOLOGY

Genetic programs constructed from layered logic gates in single cells

(2012) Tae Seok Moon et al.   NATURE

Rationally designed families of orthogonal RNA regulators of translation

(2012) Vivek K Mutalik et al.   Nature Chemical Biology

The excludon: a new concept in bacterial antisense RNA-mediated gene regulation

(2012) Nina Sesto et al.   NATURE REVIEWS MICROBIOLOGY

Antisense RNA protects mRNA from RNase E degradation by RNA–RNA duplex formation during phage infection

(2011) Damir Stazic et al.   NUCLEIC ACIDS RESEARCH

Engineering Static and Dynamic Control of Synthetic Pathways

(2010) William J. Holtz et al.   CELL

Potent Transcriptional Interference by Pausing of RNA Polymerases over a Downstream Promoter

(2009) Adam C. Palmer et al.   MOLECULAR CELL