期刊
BIORESOURCE TECHNOLOGY
卷 250, 期 -, 页码 642-649出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.10.007
关键词
Bacillus subtilis; N-acetyl-D-glucosamine; Overflow metabolism; Acetoin; Acetate
资金
- National Natural Science Foundation of China [31622001, 31671845, 21676119, 31600068]
- Natural Science Foundation of Jiangsu Province - China [BK20160176]
- Fundamental Research Funds for the Central Universities [JUSRP11725]
- 111 Project [111-2-06]
- China Postdoctoral Science Foundation - China [2016M600363, 2017T100327]
Bacillus subtilis is widely used as cell factories for the production of important industrial biochemicals. Although many studies have demonstrated the effects of organic acidic byproducts, such as acetate, on microbial fermentation, little is known about the effects of blocking the neutral byproduct overflow, such as acetoin, on bioproduction. In this study, we focused on the influences of modulating overflow metabolism on the production of N-acetyl-D-glucosamine (GlcNAc) in engineered B. subtilis. We found that acetoin overflow competes with GlcNAc production, and blocking acetoin overflow increased GlcNAc titer and yield by 1.38-and 1.39-fold, reaching 48.9 g/L and 0.32 g GlcNAc/g glucose, respectively. Further blocking acetate overflow inhibited cell growth and GlcNAc production may be induced by inhibiting glucose uptake. Taken together, our results show that blocking acetoin overflow is a promising strategy for enhancing GlcNAc production. The strategies developed in this work may be useful for engineering strains of B. subtilis for producing other important biochemicals.
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