Article
Plant Sciences
Yang Xu, Kun Zhang, Shuhao Li, Yuqi Zhou, Shengxiang Ran, Ru Xu, Yizhang Lin, Li Shen, Weiqun Huang, Fenglin Zhong
Summary: A hydroponic experiment was conducted to investigate the effects of different nitrogen supply levels on tomato leaf carbohydrate and nitrogen metabolism. The results showed that as nitrogen concentration increased, starch and sucrose content initially increased and then decreased, while glucose and fructose content decreased. Enzyme activity of Glutamate dehydrogenase tended to increase and then decrease, while NADH-Glutamate synthase showed the opposite trend. Transcriptome analysis identified 6383 differentially expressed genes involved in various metabolic pathways, providing insights into the mechanisms of nitrogen metabolism in tomato.
PLANT GROWTH REGULATION
(2023)
Article
Environmental Sciences
Mohd Asgher, Zebus Sehar, Abdul Rehaman, Shaista Rashid, Sajad Ahmed, Tasir S. Per, Mohammed Nasser Alyemeni, Nafees A. Khan
Summary: L-Glutamic acid has been found to be an effective bio-stimulant in reducing arsenic stress in rice plants. The supplementation of Glu at an appropriate concentration can enhance photosynthesis and growth by increasing antioxidant enzymes, promoting proline metabolism, and facilitating nitrogen assimilation. This study provides insights into the role of Glu as a signaling molecule in mitigating the adverse effects of arsenic stress.
ENVIRONMENTAL POLLUTION
(2022)
Article
Plant Sciences
Badar Jahan, Noushina Iqbal, Mehar Fatma, Zebus Sehar, Asim Masood, Adriano Sofo, Ilaria D'Ippolito, Nafees A. Khan
Summary: The study showed that ethylene as ethephon is effective in alleviating the damaging effects of salt stress on mustard plants, especially when used in combination with split doses of nitrogen and sulfur treatments. The application of ethylene significantly enhanced photosynthetic efficiency, promoted the assimilation of nitrogen and sulfur, and increased antioxidant activity in the plants.
Article
Environmental Sciences
Linglin Xu, Zhiheng Li, Biyan Zhuang, Fumin Zhou, Zejun Li, Xiaoru Pan, Hao Xi, Wenlu Zhao, Huijun Liu
Summary: The study found that enrofloxacin at high concentration inhibits rice seedling growth, increases nitrate uptake, and enhances nitrogen content. Additionally, enrofloxacin promotes the activity of enzymes related to nitrogen assimilation, leading to the production of more amino acids and proteins, thereby altering the plant's phenotype.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Plant Sciences
Tiara da Silva Coelho-Bortolo, Rogerio Marchiosi, Ana Paula Ferro, Rita de Cassia Siqueira-Soares, Rodrigo Polimeni Constantin, Wanderley Dantas dos Santos, Osvaldo Ferrarese-Filho
Summary: l-DOPA affects the ability of soybean plants to uptake and assimilate inorganic nitrogen into amino acids. It inhibits the uptake and conversion of nitrate and ammonium and leads to the accumulation of free amino acids in the roots and leaves.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Jiajia Li, Xinyu Liu, Lingqing Xu, Wangsheng Li, Qi Yao, Xilong Yin, Qiuhong Wang, Wenbo Tan, Wang Xing, Dali Liu
Summary: Low nitrogen-tolerant sugar beet reduces energy expenditure by lowering the synthesis of energy-consuming amino acids and promotes glutathione metabolism to protect cells from oxidative damage. The expression levels of nitrogen assimilation and amino acid transport genes play a decisive role in nitrogen utilization and may affect carbon metabolism. There may be corresponding remote regulation between flavonoid biosynthesis in roots and nitrogen metabolism in leaves.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Dandan Tang, Zixin Jiao, Qunfeng Zhang, Mei-Ya Liu, Jianyun Ruan
Summary: Glutamate dehydrogenase (GDH) plays a central role in nitrogen metabolism in tea plants, with three isogenes identified. The cooperation between CsGDHs and CsGSs in ammonium assimilation is more pronounced under high ammonium conditions. Additionally, CsGDHs are found to be crucial in ammonium assimilation in chlorotic tea leaves, suggesting their important functions in nitrogen metabolism.
Review
Biochemistry & Molecular Biology
Mei Han, Can Zhang, Peter Suglo, Shuyue Sun, Mingyao Wang, Tao Su
Summary: The review focuses on the importance of L-aspartate in plants, covering its biosynthesis, catabolism, and role in response to environmental changes, with an emphasis on its distribution in cell compartments and significance in the entire plant under stress conditions. It also discusses the interconnection between L-aspartate and phytohormones, which play crucial roles in plant growth, development, and defense.
Review
Biochemistry & Molecular Biology
Xiujie Liu, Bin Hu, Chengcai Chu
Summary: Nitrogen is essential for crop yields, but excessive application can lead to increased production costs and environmental problems. Understanding the molecular mechanisms of nitrogen use efficiency (NUE) and breeding crops with higher NUE is crucial. NUE is determined by nitrogen uptake, transport, assimilation, and remobilization, involving key enzymes like NR, NiR, GS, and GOGAT.
JOURNAL OF GENETICS AND GENOMICS
(2022)
Article
Plant Sciences
M. M. Aci, A. Lupini, A. Mauceri, F. Sunseri, M. R. Abenavoli
Summary: The study revealed that under short-term nitrate limiting supply, the genotype RO showed higher nitrate storage and remobilization abilities compared to UC82, while under long-term limiting conditions, the two genotypes exhibited differential gene expression levels related to nitrate assimilation and allocation. These results suggest that the Nitrogen Utilization Efficiency component plays a central role in enhancing Nitrogen Use Efficiency in tomato plants.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Jie Li, Tao Wu, Ke Huang, Yubing Liu, Mingyue Liu, Junwei Wang
Summary: Light quality optimization is an efficient method for improving the growth and quality of lettuce, with the addition of green, purple, and far-red light leading to increased vitamin C content and nitrogen metabolism conversion. The results suggest that light quality regulates nitrate reduction by influencing gene expression and enzyme activity related to nitrogen metabolism.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Liuqing Huo, Zijian Guo, Qi Wang, Li Cheng, Xin Jia, Ping Wang, Xiaoqing Gong, Cuiying Li, Fengwang Ma
Summary: Overexpression of MdATG10 enhances salt tolerance in apple plants by promoting autophagic activity in roots. MdATG10 expression is induced by nitrogen depletion, leading to increased nitrogen absorption and assimilation. These transgenic plants maintain higher photosynthetic ability and better growth under nitrogen starvation stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Dandan Li, Jianxiu Liu, Hailin Guo, Junqin Zong, Jianjian Li, Jingjing Wang, Ling Li, Jingbo Chen
Summary: This study found that low N tolerant wild bermudagrass accessions were able to adapt to low N conditions by improving root N uptake ability and increasing N remobilization in the shoots, thereby maintaining a better N status. These findings help elucidate the low N tolerance mechanisms in bermudagrass and can facilitate genetic improvement of N use efficiency for low-input turfgrass management.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Shahidul Islam, Jingjuan Zhang, Yun Zhao, Maoyun She, Wujun Ma
Summary: Increasing NUE is crucial for crop yield but faces challenges due to the complexity of nitrogen processes and genetic regulation. Recent research focuses on transcription factors and miRNAs regulating NUE-related genes. Integrating knowledge from model species and exploring genetic variations in wheat may lead to effectively improving NUE levels.
Article
Biochemistry & Molecular Biology
Yihao Wei, Lulu Wang, Butan Qin, Huiqiang Li, Xiaoran Wang, Zhiyong Zhang, Xiaobo Zhu, Xinming Ma, Xiaochun Wang
Summary: The study investigated the spatiotemporal expression of the TaGS gene family after anthesis, highlighting the potential role of TaGS1;2 in nitrogen remobilization by assimilating NH4+ released from ureide degradation in the phloem of peduncle and rachis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Kristin Surmann, Marius Stopp, Sebastian Woerner, Vishnu M. Dhople, Uwe Voelker, Gottfried Unden, Elke Hammer
JOURNAL OF PROTEOMICS
(2020)
Article
Biochemistry & Molecular Biology
Robin Klein, Ann-Katrin Kretzschmar, Gottfried Unden
MOLECULAR MICROBIOLOGY
(2020)
Article
Microbiology
Gottfried Unden, Robin Klein
Summary: Bacteria have evolved widely different strategies for coordinating the response to O-2 and nitrate, with many species using individual regulation for O-2 and nitrate, while others utilize joint sensing proteins to sense both oxygen and nitrate at the same time.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marius Stopp, Philipp Aloysius Steinmetz, Christopher Schubert, Christian Griesinger, Dirk Schneider, Gottfried Unden
Summary: The study demonstrates that the transmembrane helix TM2 of the DcuS sensor kinase forms a stable homodimer in both inactive and fumarate-activated states, with a conserved motif crucial for stability and function. TM2 helix extends into the PASP domain and PAS(C) domain, transmitting signals to the kinase domain. Structural rearrangements in a helical linker connecting TM2 with PAS(C) upon fumarate activation suggest piston-type transmembrane signaling.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Microbiology
Christopher Schubert, Maria Winter, Andrea Ebert-Jung, Sylwia Kierszniowska, Kerstin Nagel-Wolfrum, Thorben Schramm, Hannes Link, Sebastian Winter, Gottfried Unden
Summary: The study found that C4-dicarboxylates and fumarate respiration play important roles in the colonization of the mammalian intestinal tract by Escherichia coli, with L-aspartate being crucial for providing fumarate and nitrogen for bacterial growth in the mouse intestine. Mutants with defective anaerobic C4-dicarboxylate metabolism were impaired in colonizing the murine gut.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marius Stopp, Philipp A. Steinmetz, Gottfried Unden
Summary: The sensor kinase DcuS of Escherichia coli recognizes extracellular fumarate through a periplasmic PASP sensor domain. The TM1 helix of DcuS forms a homo-dimer with an α-helical arrangement in the central membrane region, and a non-α-helical arrangement in the connector to PASP. The structural adaptation in response to the shift of TM2 is suggested to occur in the V32-S42 region of TM1.
BIOLOGICAL CHEMISTRY
(2021)
Article
Microbiology
Marius Stopp, Christopher Schubert, Gottfried Unden
Summary: This study identified the interaction site in the membrane-bound C-4-dicarboxylate (C4DC) sensor kinase DcuS of Escherichia coli and the related structural and functional adaptations in DcuS. The stabilization of the linker-linker' homodimer in DcuS is essential for setting DcuS in the C4DC-responsive state in the DctA x DcuS complex, with DctA serving as a structural co-regulator of DcuS sensor kinase.
Review
Microbiology
Christopher Schubert, Gottfried Unden
Summary: C4-dicarboxylates play a significant role in the colonization of enteric bacteria in the mammalian intestine and in the adaptation of host physiology, with L-aspartate being crucial for fumarate respiration and nitrogen assimilation, and succinate functioning as a signaling molecule for host-enteric bacterium interaction.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Microbiology
Stefaniya Gencheva, Simon Dersch, Kristin Surmann, Mathias Wernet, Luis Antelo, Elke Hammer, Peter L. Graumann, Nadja Hellmann, Gottfried Unden
Summary: This study investigates the interaction between the C4-dicarboxylate-responsive and membrane-integral sensor kinase DcuS and the response regulator DcuR. The formation of stable complexes is influenced by the phosphorylation status of the proteins. Single-molecule tracking shows two populations of DcuR with different mobilities in bacterial cells, one at the membrane and the other in the cytosol, possibly representing DNA-bound DcuR. The findings suggest that the interaction between DcuS and DcuR plays a crucial role in cellular signaling.
Article
Microbiology
Christopher Schubert, Nam Yeun Kim, Gottfried Unden, Ok Bin Kim
Summary: The study revealed the presence of various metabolons in Escherichia coli, with different functions. Metabolons were observed for common C-4-dicarboxylate transporters with cytosolic enzymes, while no metabolons were observed for noncommon C-4-dicarboxylates.
FEMS MICROBIOLOGY LETTERS
(2022)