期刊
ENVIRONMENTAL MICROBIOLOGY
卷 23, 期 5, 页码 2564-2577出版社
WILEY
DOI: 10.1111/1462-2920.15478
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资金
- Deutsche Forschungsgemeinschaft [UN 49/19-1]
- Welch Foundation [I-1969-20180324]
- NIH [AI118807, AI128151]
- Burroughs Wellcome Fund [1017880]
- American Cancer Society [RSG-17-048-01-MPC]
The study found that C4-dicarboxylates and fumarate respiration play important roles in the colonization of the mammalian intestinal tract by Escherichia coli, with L-aspartate being crucial for providing fumarate and nitrogen for bacterial growth in the mouse intestine. Mutants with defective anaerobic C4-dicarboxylate metabolism were impaired in colonizing the murine gut.
C4-dicarboxylates, such as fumarate, l-malate and l-aspartate represent substrates for anaerobic growth of Escherichia coli by fumarate respiration. Here, we determined whether C4-dicarboxylate metabolism, as well as fumarate respiration, contribute to colonization of the mammalian intestinal tract. Metabolite profiling revealed that the murine small intestine contained high and low levels of l-aspartate and l-malate respectively, whereas fumarate was nearly absent. Under laboratory conditions, addition of C4-dicarboxylate at concentrations corresponding to the levels of the C4-dicarboxylates in the small intestine (2.6 mmol kg(-1) dry weight) induced the dcuBp-lacZ reporter gene (67% of maximal) in a DcuS-DcuR-dependent manner. In addition to its role as a precursor for fumarate respiration, l-aspartate was able to supply all the nitrogen required for anaerobically growing E. coli. DcuS-DcuR-dependent genes were transcribed in the murine intestine, and mutants with defective anaerobic C4-dicarboxylate metabolism (dcuSR, frdA, dcuB, dcuA and aspA genes) were impaired for colonizing the murine gut. We conclude that l-aspartate plays an important role in providing fumarate for fumarate respiration and supplying nitrogen for E. coli in the mouse intestine.
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