Article
Biochemistry & Molecular Biology
Zsolt Fejes, Marianna Pocsi, Jun Takai, Judit Erdei, Andrea Toth, Eniko Balogh, Agnes Rusznyak, Ferenc Fenyvesi, Andrea Nagy, Janos Kappelmayer, Viktoria Jeney, Bela Nagy
Summary: The study revealed a time-dependent increase in Hb derivatives, IL-8, and adhesion molecules in IVH-IV compared to IVH-III, with higher expression of miR-155 and miR-181b at days 41-60. Exposure of HCPEpiCs to IVH CSF samples resulted in increased levels of HMOX1, IL8, and ICAM1 mRNA, along with ROS production via NF-kappa B pathway activation, without causing cell death. Additionally, higher intracellular miR-155 levels and decreased miR-223 and miR-181b expression were observed in HCPEpiCs after treatment with CSF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Wendy C. Ziai, Adrian R. Parry-Jones, Carol B. Thompson, Lauren H. Sansing, Michael T. Mullen, Santosh B. Murthy, Andrew Mould, Saman Nekoovaght-Tak, Daniel F. Hanley
Summary: The study investigated the expression of inflammatory cytokines in cerebrospinal fluid and their relationship with disease severity in patients with acute IVH. Results showed that some inflammatory cytokine levels peaked earlier in IVH patients, with positive correlations with IVH and ICH volumes. Time trends of cytokines support the association of cerebral inflammatory responses with the severity of ICH/IVH.
Article
Immunology
Alex Adusei Agyemang, Suvi Vallius Kvist, Nathan Brinkman, Thomas Gentinetta, Miriam Illa, Niklas Ortenlof, Bo Holmqvist, David Ley, Magnus Gram
Summary: Cell-free hemoglobin plays a central role in the pathophysiology of GM-IVH in preterm infants, and early administration of the hemoglobin scavenger Hp may provide therapeutic benefits.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Clinical Neurology
Jennifer M. Strahle, Kelly B. Mahaney, Diego M. Morales, Chandana Buddhala, Chevis N. Shannon, John C. Wellons, Abhaya V. Kulkarni, Hailey Jensen, Ron W. Reeder, Richard Holubkov, Jay K. Riva-Cambrin, William E. Whitehead, Curtis J. Rozzelle, Mandeep Tamber, Ian F. Pollack, Robert P. Naftel, John R. W. Kestle, David D. Limbrick
Summary: In neonatal patients with PHH, longitudinal changes in CSF transferrin (increase) and ferritin (decrease) are associated with improved neurodevelopmental outcomes, providing insights into the pathogenesis of poor outcomes in PHH.
ANNALS OF NEUROLOGY
(2021)
Article
Neurosciences
Laura Ollegaard Johnsen, Kathrine Abildskov Friis, Helle Hasager Damkier
Summary: Intraventricular hemorrhage is a life-threatening condition with approximately 20% of patients developing posthemorrhagic hydrocephalus. Inflammatory components have been found to affect membrane transporters involved in cerebrospinal fluid secretion, primarily mediated by the activation of basolateral receptors in the choroid plexus, mainly facilitated by IL-6.
FLUIDS AND BARRIERS OF THE CNS
(2023)
Article
Immunology
Zhaoqi Zhang, Qiang Tan, Peiwen Guo, Suna Huang, Zhengcai Jia, Xin Liu, Hua Feng, Yujie Chen
Summary: The NLRP3 inflammasome plays a crucial role in the pathogenesis of hydrocephalus after intracerebral hemorrhage. Inhibition of NLRP3 decreases CSF secretion and attenuates ventricular dilation and neurological deficits.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Pediatrics
Luis Octavio Tierradentro-Garcia, Levy Onyango, Rebecca Dennis, Colbey W. Freeman, Sophie Haddad, Brandi Kozak, Misun Hwang
Summary: This study demonstrates that Microvascular Imaging Ultrasound (MVI) can be used to detect cerebrospinal fluid (CSF) flow dynamics in infants with a history of post-hemorrhagic hydrocephalus, with a high inter-rater reliability (IRR). The visualization of CSF flow using MVI was significantly associated with the presence of intraventricular hemorrhage (IVH) alone and IVH+hydrocephalus, but not with hydrocephalus alone.
Article
Neurosciences
Rieka M. Reger, Anton Meinicke, Wolfgang Haertig, Matthias Knuepfer, Ulrich Thome, Stefan Schob, Matthias Krause
Summary: This study aimed to evaluate changes in surface tension (ST) in children with intraventricular hemorrhage (IVH) in relation to surfactant proteins (SPs). The results showed significantly decreased ST and elevated SPs in IVH patients, suggesting that these changes are coping mechanisms to preserve cerebrospinal fluid flow. The increase in ST over time may serve as a prognostic marker for the healing process.
Article
Chemistry, Analytical
Hui-Tzung Luh, Yi-Wei Chung, Po-Yi Cho, Yu-Cheng Hsiao
Summary: A novel device for detecting HO-1 in cerebrospinal fluid (CSF) was invented using cholesteric liquid crystal (LC; CLC). The LC reorientation was influenced by the alignment layer and adjacent LCs, enabling the detection of HO-1. This label-free CLC immunoassay offers a low detection limit and a wide linear range for HO-1 concentration measurement in CSF.
Article
Immunology
Aaron M. Gusdon, Carol B. Thompson, Kendel Quirk, Yunis M. Mayasi, Radhika Avadhani, Issam A. Awad, Daniel F. Hanley, Wendy C. Ziai
Summary: The study found that in patients with ICH and IVH, treatment with alteplase compared to saline resulted in significantly enhanced inflammatory response in CSF leukocyte subtypes. Increased serum lymphocytes may be associated with better outcomes, while increased neutrophil-to-lymphocyte ratio (NLR) may be associated with worse outcomes.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Neurosciences
Sara Diana Lolansen, Nina Rostgaard, Dagne Barbuskaite, Tenna Capion, Markus Harboe Olsen, Nicolas H. Norager, Frederik Vilhardt, Soren Norge Andreassen, Trine L. Toft-Bertelsen, Fenghui Ye, Marianne Juhler, Richard F. Keep, Nanna MacAulay
Summary: This study aimed to elucidate the molecular coupling between hemorrhagic events and the development of posthemorrhagic hydrocephalus (PHH), as well as the inflammatory profile of PHH pathogenesis. The findings suggest that excessive cerebrospinal fluid (CSF) secretion may contribute to PHH development, likely due to hyperactivity of choroid plexus transporters. Hemorrhage-induced inflammation detected in CSF and choroid plexus tissue may represent the underlying pathology of PHH.
FLUIDS AND BARRIERS OF THE CNS
(2022)
Review
Pediatrics
William Dawes
Summary: Intraventricular hemorrhage is a major cause of hydrocephalus in developed countries and can significantly impact neurodevelopmental outcomes in premature infants. The underlying pathophysiology is believed to involve primary destructive and secondary developmental insults, but exact mechanisms are still unclear. Damage to the ependymal layer following IVH may adversely affect the brain microenvironment, further exacerbating disordered activity within the subventricular zone.
FRONTIERS IN PEDIATRICS
(2022)
Article
Clinical Neurology
Yi Cao, Chang Liu, Gaowei Li, Weina Gao, Hui Tang, Shuanmin Fan, Xin Tang, Long Zhao, Haoxiang Wang, Aijun Peng, Chao You, Aiping Tong, Liangxue Zhou
Summary: Metformin has been found to attenuate neuroinflammation and subsequent fibrosis after intraventricular hemorrhage, thereby reducing the occurrence of delayed hydrocephalus. It exerts its effects through regulating the AMPK/SIRT1/NF-kappa B pathways.
TRANSLATIONAL STROKE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Wang-Xia Wang, Joe E. Springer, Kevin Xie, David W. Fardo, Kevin W. Hatton
Summary: This study demonstrates the potential of a custom designed miRNA panel in predicting the risk of DCV in aSAH patients. The differential expression of selected miRNAs in CSF and plasma before the occurrence of DCV events highlights the early predictive capability of this panel. Additionally, the prediction accuracy reached 87% in a separate testing cohort, indicating the strong applications of this miRNA panel in clinical management of aSAH patients.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Multidisciplinary Sciences
Kornelia Laichinger, Paula Bombach, Jutta Duenschede, Christoph Ruschil, Maria-Ioanna Stefanou, Evelyn Dubois, Sven Poli, Katharina Feil, Ulf Ziemann, Markus Kowarik, Annerose Mengel
Summary: In this study, the prevalence of intrathecal IgG synthesis and B cell activation in AIS patients was determined. It was found that 5.7% of patients had intrathecal IgG synthesis, and one third of these patients had concurrent chronic inflammatory CNS disease. No significant correlation was found with stroke characteristics.
Article
Cell Biology
Judit Erdei, Andrea Toth, Eniko Balogh, Benard Bogonko Nyakundi, Emese Banyai, Bernhard Ryffel, Gyorgy Paragh, Mario D. Cordero, Viktoria Jeney
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2018)
Article
Cell Biology
Laszlo Potor, Peter Nagy, Gabor Mehes, Zoltan Hendrik, Viktoria Jeney, David Petho, Anita Vasas, Zoltan Palinkas, Eniko Balogh, Agnes Gyetvai, Matthew Whiteman, Roberta Torregrossa, Mark E. Wood, Sandor Olvaszto, Peter Nagy, Gyorgy Balla, Jozsef Balla
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2018)
Article
Multidisciplinary Sciences
Susana Ramos, Ana Rita Carlos, Balamurugan Sundaram, Viktoria Jeney, Ana Ribeiro, Raffaella Gozzelino, Claudia Bank, Erida Gjini, Faouzi Braza, Rui Martins, Temitope Wilson Ademolue, Birte Blankenhaus, Zelia Gouveia, Pedro Faisca, Damian Trujillo, Silvia Cardoso, Sofia Rebelo, Laura del Barrio, Abolfazl Zarjou, Subhashini Bolisetty, Anupam Agarwal, Miguel P. Soares
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biochemistry & Molecular Biology
Bernadett Szilagyi, Zsolt Fejes, Szilard Poliska, Marianna Pocsi, Zsolt Czimmerer, Andreas Patsalos, Ferenc Fenyvesi, Agnes Rusznyak, Gyorgy Nagy, Gyorgy Kerekes, Mariann Berhes, Ildiko Szucs, Satya P. Kunapuli, Janos Kappelmayer, Bela Nagy
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Pharmacology & Pharmacy
Agnes Rusznyak, Milo Malanga, Eva Fenyvesi, Lajos Szente, Judit Varadi, Ildiko Bacskay, Miklos Vecsernyes, Gabor Vasvari, Adam Haimhoffer, Palma Feher, Zoltan Ujhelyi, Bela Nagy, Zsolt Fejes, Ferenc Fenyvesi
Summary: This study compared the cellular internalization of fluorescein- and rhodamine-labeled cyclodextrin derivatives and investigated their intracellular effects on Caco-2 cells. Results showed that the type of fluorescent labeling did not affect the internalization of cyclodextrins, and these derivatives could be detected in lysosomes after internalization. Additionally, activation of NF-kappa B pathway or autophagy did not increase after treatment with cyclodextrins.
Article
Biochemistry & Molecular Biology
Harriet Ghansah, Ildiko Beke Debreceni, Zsolt Fejes, Bela Nagy, Janos Kappelmayer
Summary: The study revealed that Bortezomib (BTZ) induces a procoagulant platelet phenotype, leading to increased phosphatidylserine (PS) and P-selectin expression levels, as well as depolarization of mitochondrial inner membrane potential in gel-filtered platelets (GFPs) compared to platelet-rich plasma (PRP). The presence of plasma proteins in PRP may attenuate the procoagulant effect of BTZ on platelets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Zsolt Fejes, Marianna Pocsi, Jun Takai, Judit Erdei, Andrea Toth, Eniko Balogh, Agnes Rusznyak, Ferenc Fenyvesi, Andrea Nagy, Janos Kappelmayer, Viktoria Jeney, Bela Nagy
Summary: The study revealed a time-dependent increase in Hb derivatives, IL-8, and adhesion molecules in IVH-IV compared to IVH-III, with higher expression of miR-155 and miR-181b at days 41-60. Exposure of HCPEpiCs to IVH CSF samples resulted in increased levels of HMOX1, IL8, and ICAM1 mRNA, along with ROS production via NF-kappa B pathway activation, without causing cell death. Additionally, higher intracellular miR-155 levels and decreased miR-223 and miR-181b expression were observed in HCPEpiCs after treatment with CSF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Eniko Szekely, Gizella Csire, Bettina Diana Balogh, Judit Zsuzsa Erdei, Judit Maria Kiraly, Judit Kocsi, Julia Pinkoczy, Katalin Varnagy
Summary: The systematic studies of copper(II), nickel(II) and zinc(II) ion complexes of protected multihistidine peptides have provided insights into the metal ion and protein interaction. The stability of the complexes is influenced by the position of histidines and neighboring amino acids, with aspartic acid and phenylalanine having the most significant effect. The redox parameters of the complexes depend on the number and position of histidines, while other side chain donor atoms have minimal impact. However, the presence of aspartic acid side chains leads to a more distorted geometry and increased reducibility of the complexes.
Article
Cell Biology
Adam Feher, Marianna Pocsi, Ferenc Papp, Tibor G. Szanto, Agota Csoti, Zsolt Fejes, Bela Nagy, Balazs Nemes, Zoltan Varga
Summary: This study identified multiple voltage-gated sodium channels in the plasma membrane of human B cells using electrophysiological and molecular biology methods. The detected sodium currents showed sensitivity between TTX-sensitive and TTX-insensitive channels, suggesting the co-existence of multiple Na(V)1 subtypes. The results also supported the functional role of these channels in shaping and maintaining the resting membrane potential.
Article
Biochemistry & Molecular Biology
Jozsef Kiraly, Erzsebet Szabo, Petra Fodor, Zsolt Fejes, Bela Nagy Jr, Eva Juhasz, Anna Vass, Mahua Choudhury, Gabor Konya, Gabor Halmos, Zsuzsanna Szabo
Summary: Shikonin induces apoptosis of renal cancer cells by activating the Ras/MAPK and PI3K/AKT pathways, suggesting its potential therapeutic implications for the treatment of renal cancer.