One-step genome editing of porcine zygotes through the electroporation of a CRISPR/Cas9 system with two guide RNAs

Evaluation of multiple gene targeting in porcine embryos by the CRISPR/Cas9 system using electroporation

(2020) Maki Hirata et al.   MOLECULAR BIOLOGY REPORTS

Genome mutation after introduction of the gene editing by electroporation of Cas9 protein (GEEP) system in matured oocytes and putative zygotes

(2019) Maki Hirata et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

The Relationship between Embryonic Development and the Efficiency of Target Mutations in Porcine Endogenous Retroviruses (PERVs) Pol Genes in Porcine Embryos

(2019) Maki Hirata et al.   Animals

Electroporation-mediated genome editing in vitrified/warmed mouse zygotes created by IVF via ultra-superovulation

(2018) Yoshiko NAKAGAWA et al.   EXPERIMENTAL ANIMALS

Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer

(2018) Honghao Yu et al.   Journal of Translational Medicine

Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

(2018) Michael Kosicki et al.   NATURE BIOTECHNOLOGY

CRISPR off-target analysis in genetically engineered rats and mice

(2018) Keith R. Anderson et al.   NATURE METHODS

Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation

(2018) Fuminori Tanihara et al.   PLoS One

In situ immune response and mechanisms of cell damage in central nervous system of fatal cases microcephaly by Zika virus

(2018) Raimunda S. S. Azevedo et al.   Scientific Reports

Generation of PDX-1 mutant porcine blastocysts by introducing CRISPR/Cas9-system into porcine zygotes via electroporation

(2018) Fuminori Tanihara et al.   ANIMAL SCIENCE JOURNAL

Chlorogenic acid supplementation during in vitro maturation improves maturation, fertilization and developmental competence of porcine oocytes

(2017) T-V Nguyen et al.   REPRODUCTION IN DOMESTIC ANIMALS

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes

(2016) Sean Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Biallelic modification of IL2RG leads to severe combined immunodeficiency in pigs

(2016) Jung-Taek Kang et al.   Reproductive Biology and Endocrinology

Increased and prolonged human norovirus infection in RAG2/IL2RG deficient gnotobiotic pigs with severe combined immunodeficiency

(2016) Shaohua Lei et al.   Scientific Reports

Somatic cell reprogramming-free generation of genetically modified pigs

(2016) F. Tanihara et al.   Science Advances

Ultrastructural Characterization of the Lower Motor System in a Mouse Model of Krabbe Disease

(2016) Valentina Cappello et al.   Scientific Reports

CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

(2014) Yuki Naito et al.   BIOINFORMATICS

Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos

(2014) Yuyu Niu et al.   CELL

Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer

(2014) Xiaoqing Zhou et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

The multilayered complexity of ceRNA crosstalk and competition

(2014) Yvonne Tay et al.   NATURE

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons

(2014) Zoltán Ivics et al.   Nature Protocols

Easy quantitative assessment of genome editing by sequence trace decomposition

(2014) Eva K. Brinkman et al.   NUCLEIC ACIDS RESEARCH

Enhanced Specificity and Efficiency of the CRISPR/Cas9 System with Optimized sgRNA Parameters in Drosophila

(2014) Xingjie Ren et al.   Cell Reports

COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites

(2014) Thomas J Cradick et al.   Molecular Therapy-Nucleic Acids

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Generation of Interleukin-2 Receptor Gamma Gene Knockout Pigs from Somatic Cells Genetically Modified by Zinc Finger Nuclease-Encoding mRNA

(2013) Masahito Watanabe et al.   PLoS One

Pig transgenesis by piggyBac transposition in combination with somatic cell nuclear transfer

(2013) Zhenfang Wu et al.   TRANSGENIC RESEARCH

Il2rg Gene-Targeted Severe Combined Immunodeficiency Pigs

(2012) Shunichi Suzuki et al.   Cell Stem Cell

Development of Transgenic Cloned Pig Models of Skin Inflammation by DNA Transposon-Directed Ectopic Expression of Human β1 and α2 Integrin

(2012) Nicklas Heine Staunstrup et al.   PLoS One

Characterization of the ICSI-mediated gene transfer method in the production of transgenic pigs

(2011) Kazuhiro Umeyama et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

Gene silencing in bovine zygotes: siRNA transfection versus microinjection

(2011) Ciara M. O'Meara et al.   REPRODUCTION FERTILITY AND DEVELOPMENT

The pig: a model for human infectious diseases

(2011) François Meurens et al.   TRENDS IN MICROBIOLOGY

Cytoplasmic injection of circular plasmids allows targeted expression in mammalian embryos

(2009) Khursheed Iqbal et al.   BIOTECHNIQUES