Current problems and future avenues in proteoliposome research

New approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteins

(2020) Gabriella Angiulli et al.   eLife

Bifunctional DNA duplexes permit efficient incorporation of pH probes into liposomes

(2020) Nicolas Dolder et al.   CHEMBIOCHEM

Membrane Lipid Requirements of the Lysine Transporter Lyp1 from Saccharomyces cerevisiae

(2020) Joury S. van 't Klooster et al.   JOURNAL OF MOLECULAR BIOLOGY

Bottom-Up Construction of a Minimal System for Cellular Respiration and Energy Regeneration

(2020) Olivier Biner et al.   ACS Synthetic Biology

Constructing artificial respiratory chain in polymer compartments: Insights into the interplay betweenbo3oxidase and the membrane

(2020) Nika Marušič et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Phospholipid distribution in the cytoplasmic membrane of Gram-negative bacteria is highly asymmetric, dynamic, and cell shape-dependent

(2020) Mikhail Bogdanov et al.   Science Advances

Using Imaging Flow Cytometry to Quantify and Optimize Giant Vesicle Production by Water-in-oil Emulsion Transfer Methods

(2019) Yuka Matsushita-Ishiodori et al.   LANGMUIR

Insertion and folding pathways of single membrane proteins guided by translocases and insertases

(2019) Tetiana Serdiuk et al.   Science Advances

Using Fluorescence Quenching Titration to Determine the Orientation of a Model Transmembrane Protein in Mimic Membranes

(2019) Haihong Huang et al.   Materials

Artificial photosynthetic cell producing energy for protein synthesis

(2019) Samuel Berhanu et al.   Nature Communications

Dimers of mitochondrial ATP synthase induce membrane curvature and self-assemble into rows

(2019) Thorsten B. Blum et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering of Nucleic Acids and Synthetic Cofactors as Holo Sensors for Probing Signaling Molecules in the Cellular Membrane Microenvironment

(2019) Guangfu Feng et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

An Integrated Microfluidic Platform for Quantifying Drug Permeation across Biomimetic Vesicle Membranes

(2019) Michael Schaich et al.   MOLECULAR PHARMACEUTICS

Lipid-Assisted Membrane Protein Folding and Topogenesis

(2019) William Dowhan et al.   PROTEIN JOURNAL

One-Pot Assembly of Complex Giant Unilamellar Vesicle-Based Synthetic Cells

(2019) Kerstin Göpfrich et al.   ACS Synthetic Biology

Fabrics of Diverse Chemistries Promote the Formation of Giant Vesicles from Phospholipids and Amphiphilic Block Copolymers

(2019) Vaishnavi Girish et al.   LANGMUIR

Building a synthetic mechanosensitive signaling pathway in compartmentalized artificial cells

(2019) James W. Hindley et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell-Surface-Anchored Ratiometric DNA Nanoswitch for Extracellular ATP Imaging

(2019) Jing Yuan et al.   ACS Sensors

Expression and detergent free purification and reconstitution of the plant plasma membrane Na+/H+ antiporter SOS1 overexpressed in Pichia pastoris

(2019) Debajyoti Dutta et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Stairway to Asymmetry: Five Steps to Lipid-Asymmetric Proteoliposomes

(2019) Marie Markones et al.   BIOPHYSICAL JOURNAL

Structure and Dynamics of the Rhomboid Protease GlpG in Liposomes Studied by Solid-State NMR

(2019) Chaowei Shi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A synthetic metabolic network for physicochemical homeostasis

(2019) Tjeerd Pols et al.   Nature Communications

The lipid-dependent structure and function of LacY can be recapitulated and analyzed in phospholipid-containing detergent micelles

(2019) Heidi Vitrac et al.   Scientific Reports

Electrophysiology, Unplugged: Imaging Membrane Potential with Fluorescent Indicators

(2019) Pei Liu et al.   ACCOUNTS OF CHEMICAL RESEARCH

New Structures Reveal Interaction Dynamics in Respiratory Supercomplexes

(2019) Peter Brzezinski   TRENDS IN BIOCHEMICAL SCIENCES

Towards a Synthetic Mitochondrion

(2018) Olivier Biner et al.   CHIMIA

Tailoring the appearance: what will synthetic cells look like?

(2018) Willem Kasper Spoelstra et al.   CURRENT OPINION IN BIOTECHNOLOGY

Methods of reconstitution to investigate membrane protein function

(2018) Ruth Skrzypek et al.   METHODS

Photosynthetic artificial organelles sustain and control ATP-dependent reactions in a protocellular system

(2018) Keel Yong Lee et al.   NATURE BIOTECHNOLOGY

Solution NMR structure of yeast Rcf1, a protein involved in respiratory supercomplex formation

(2018) Shu Zhou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cholesterol-Bearing Fluorescent G-Quadruplex Potassium Probes for Anchoring at the Langmuir Monolayer and Cell Membrane

(2018) Angelika Świtalska et al.   SENSORS

Current Challenges in Elucidating Respiratory Supercomplexes in Mitochondria: Methodological Obstacles

(2018) Sehwan Jang et al.   Frontiers in Physiology

Cell Surface-Anchored DNA Nanomachine for Dynamically Tunable Sensing and Imaging of Extracellular pH

(2018) Lan Liu et al.   ANALYTICAL CHEMISTRY

Lipids modulate the insertion and folding of the nascent chains of alpha helical membrane proteins

(2018) Nicola J. Harris et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Liposomes and polymersomes: a comparative review towards cell mimicking

(2018) Emeline Rideau et al.   CHEMICAL SOCIETY REVIEWS

The lipid environment determines the activity of the Escherichia coli ammonium transporter AmtB

(2018) Gaëtan Dias Mirandela et al.   FASEB JOURNAL

Membrane functionalization of polymersomes: alleviating mass transport limitations by integrating multiple selective membrane transporters for the diffusion of chemically diverse molecules

(2018) Tom S Schwarzer et al.   NANOTECHNOLOGY

Preparation of asymmetric phospholipid vesicles for use as cell membrane models

(2018) Milka Doktorova et al.   Nature Protocols

Extraction and liposome reconstitution of membrane proteins with their native lipids without the use of detergents

(2018) Irina A. Smirnova et al.   Scientific Reports

The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution

(2018) Michael Luke Carlson et al.   eLife

Cell-Surface-Anchored Ratiometric DNA Tweezer for Real-Time Monitoring of Extracellular and Apoplastic pH

(2018) Shu Zeng et al.   ANALYTICAL CHEMISTRY

Direct protein-lipid interactions shape the conformational landscape of secondary transporters

(2018) Chloe Martens et al.   Nature Communications

Single Proteoliposomes with E. coli Quinol Oxidase: Proton Pumping without Transmembrane Leaks

(2017) Johan Berg et al.   ISRAEL JOURNAL OF CHEMISTRY

Proteo-lipobeads to encapsulate cytochrome c oxidase from Paracoccus denitrificans

(2017) Andreas F. Geiss et al.   JOURNAL OF COLLOID AND INTERFACE SCIENCE

Oriented Reconstitution of the Full-Length KcsA Potassium Channel in a Lipid Bilayer for AFM Imaging

(2017) Ayumi Sumino et al.   Journal of Physical Chemistry Letters

Structure-Function Dissection of Pseudorabies Virus Glycoprotein B Fusion Loops

(2017) Melina Vallbracht et al.   JOURNAL OF VIROLOGY

The interactome of intact mitochondria by cross-linking mass spectrometry provides evidence for coexisting respiratory supercomplexes

(2017) Fan Liu et al.   MOLECULAR & CELLULAR PROTEOMICS

Toward Artificial Mitochondrion: Mimicking Oxidative Phosphorylation in Polymer and Hybrid Membranes

(2017) Lado Otrin et al.   NANO LETTERS

Synthetic beta cells for fusion-mediated dynamic insulin secretion

(2017) Zhaowei Chen et al.   Nature Chemical Biology

Stable membrane orientations of small dual-topology membrane proteins

(2017) Nir Fluman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dissecting the proton transport pathway in electrogenic Na+/H+antiporters

(2017) Povilas Uzdavinys et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

What can be learned about the enzyme ATPase from single-molecule studies of its subunit F1?

(2017) Sándor Volkán-Kacso et al.   QUARTERLY REVIEWS OF BIOPHYSICS

Crystallogenesis of Membrane Proteins Mediated by Polymer-Bounded Lipid Nanodiscs

(2017) Jana Broecker et al.   STRUCTURE

Stimuli-responsive liposomes for drug delivery

(2017) Y. Lee et al.   Wiley Interdisciplinary Reviews-Nanomedicine and Nanobiotechnology

Biocatalysis in Polymersomes: Improving Multienzyme Cascades with Incompatible Reaction Steps by Compartmentalization

(2017) Ludwig Klermund et al.   ACS Catalysis

Production of Isolated Giant Unilamellar Vesicles under High Salt Concentrations

(2017) Hannah Stein et al.   Frontiers in Physiology

Ratiometric fluorescence imaging of cell surface pH by poly(ethylene glycol)-phospholipid conjugated with fluorescein isothiocyanate

(2017) Ryuichi Ohgaki et al.   Scientific Reports

An Amphiphilic Fluorescent Probe Designed for Extracellular Visualization of Nitric Oxide Released from Living Cells

(2016) Hui-Wen Yao et al.   ANALYTICAL CHEMISTRY

Supramolecular Organization of Respiratory Complexes

(2016) José Antonio Enríquez   Annual Review of Physiology

Artificial membranes for membrane protein purification, functionality and structure studies

(2016) M. J. Parmar et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Three-Dimensional Distribution of Phospholipids in Gram Negative Bacteria

(2016) Samuel Furse et al.   BIOCHEMISTRY

Mimicking respiratory phosphorylation using purified enzymes

(2016) Christoph von Ballmoos et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Isolation of yeast complex IV in native lipid nanodiscs

(2016) Irina A. Smirnova et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Oriented Membrane Protein Reconstitution into Tethered Lipid Membranes for AFM Force Spectroscopy

(2016) Anna M. Bronder et al.   BIOPHYSICAL JOURNAL

Effect of Polymer Composition and pH on Membrane Solubilization by Styrene-Maleic Acid Copolymers

(2016) Stefan Scheidelaar et al.   BIOPHYSICAL JOURNAL

A membrane-anchored fluorescent probe for detecting K+ in the cell microenvironment

(2016) Mengyi Xiong et al.   CHEMICAL COMMUNICATIONS

Durable proteo-hybrid vesicles for the extended functional lifetime of membrane proteins in bionanotechnology

(2016) Sanobar Khan et al.   CHEMICAL COMMUNICATIONS

A cell-surface-anchored ratiometric i-motif sensor for extracellular pH detection

(2016) Le Ying et al.   CHEMICAL COMMUNICATIONS

Small molecule fluorescent voltage indicators for studying membrane potential

(2016) Evan W Miller   CURRENT OPINION IN CHEMICAL BIOLOGY

Pharmaceutical liposomal drug delivery: a review of new delivery systems and a look at the regulatory landscape

(2016) Claudia Zylberberg et al.   DRUG DELIVERY

Membrane protein reconstitution into giant unilamellar vesicles: a review on current techniques

(2016) Ida Louise Jørgensen et al.   EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Delivery of membrane proteins into small and giant unilamellar vesicles by charge-mediated fusion

(2016) Olivier Biner et al.   FEBS LETTERS

Nano carriers for drug transport across the blood–brain barrier

(2016) Xinming Li et al.   JOURNAL OF DRUG TARGETING

Formation of stable nanodiscs by bihelical apolipoprotein A-I mimetic peptide

(2016) Hirokazu Kariyazono et al.   JOURNAL OF PEPTIDE SCIENCE

YidC assists the stepwise and stochastic folding of membrane proteins

(2016) Tetiana Serdiuk et al.   Nature Chemical Biology

Direct observation of proton pumping by a eukaryotic P-type ATPase

(2016) S. Veshaguri et al.   SCIENCE

Molecular mechanism of cardiolipin-mediated assembly of respiratory chain supercomplexes

(2016) C. Arnarez et al.   Chemical Science

A modular platform for one-step assembly of multi-component membrane systems by fusion of charged proteoliposomes

(2016) Robert R. Ishmukhametov et al.   Nature Communications

Octanol-assisted liposome assembly on chip

(2016) Siddharth Deshpande et al.   Nature Communications

Ultrastructural Characterization of the Lower Motor System in a Mouse Model of Krabbe Disease

(2016) Valentina Cappello et al.   Scientific Reports

Controlled Co-reconstitution of Multiple Membrane Proteins in Lipid Bilayer Nanodiscs Using DNA as a Scaffold

(2015) Thomas Raschle et al.   ACS Chemical Biology

Lipid-conjugated fluorescent pH sensors for monitoring pH changes in reconstituted membrane systems

(2015) Gerdi Christine Kemmer et al.   ANALYST

Glucose transport machinery reconstituted in cell models

(2015) Jesper S. Hansen et al.   CHEMICAL COMMUNICATIONS

The styrene–maleic acid copolymer: a versatile tool in membrane research

(2015) Jonas M. Dörr et al.   EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Unidirectional incorporation of a bacterial mechanosensitive channel into liposomal membranes

(2015) Takeshi Nomura et al.   FASEB JOURNAL

bSUM: A bead-supported unilamellar membrane system facilitating unidirectional insertion of membrane proteins into giant vesicles

(2015) Hui Zheng et al.   JOURNAL OF GENERAL PHYSIOLOGY

Single Enzyme Experiments Reveal a Long-Lifetime Proton Leak State in a Heme-Copper Oxidase

(2015) Mengqiu Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Silica Nanoparticles for the Oriented Encapsulation of Membrane Proteins into Artificial Bilayer Lipid Membranes

(2015) Florian Schadauer et al.   LANGMUIR

A Cell-Surface-Anchored Ratiometric Fluorescent Probe for Extracellular pH Sensing

(2014) Guoliang Ke et al.   ACS Applied Materials & Interfaces

Liposomes as carriers of hydrophilic small molecule drugs: Strategies to enhance encapsulation and delivery

(2014) Josimar Oliveira Eloy et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

Cell Membrane-Anchored Biosensors for Real-Time Monitoring of the Cellular Microenvironment

(2014) Liping Qiu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Membrane Interactions of Fusogenic Coiled-Coil Peptides: Implications for Lipopeptide Mediated Vesicle Fusion

(2014) Martin Rabe et al.   LANGMUIR

Detergent-free isolation, characterization, and functional reconstitution of a tetrameric K+channel: The power of native nanodiscs

(2014) Jonas M. Dörr et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

SNARE-fusion mediated insertion of membrane proteins into native and artificial membranes

(2014) Gustav Nordlund et al.   Nature Communications

Identification of giant unilamellar vesicles with permeability to small charged molecules

(2014) Koji Nishimura et al.   RSC Advances

Functional role of cardiolipin in mitochondrial bioenergetics

(2013) Giuseppe Paradies et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Lipid Bilayer Composition Can Influence the Orientation of Proteorhodopsin in Artificial Membranes

(2013) Ramya Tunuguntla et al.   BIOPHYSICAL JOURNAL

The Mechanism of Detergent Solubilization of Lipid Bilayers

(2013) Dov Lichtenberg et al.   BIOPHYSICAL JOURNAL

Reconstitution of Membrane Proteins into Model Membranes: Seeking Better Ways to Retain Protein Activities

(2013) Hsin-Hui Shen et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

A two-domain elevator mechanism for sodium/proton antiport

(2013) Chiara Lee et al.   NATURE

Layer-by-layer cell membrane assembly

(2013) Sandro Matosevic et al.   Nature Chemistry

Detergent-mediated incorporation of transmembrane proteins in giant unilamellar vesicles with controlled physiological contents

(2013) M. Dezi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Substrate selectivity in arginine-dependent acid resistance in enteric bacteria

(2013) M.-F. Tsai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

GRecon: A Method for the Lipid Reconstitution of Membrane Proteins

(2012) Thorsten Althoff et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The Membrane Modulates Internal Proton Transfer in Cytochrome c Oxidase

(2012) Linda Näsvik Öjemyr et al.   BIOCHEMISTRY

A novel leaflet-selective fluorescence labeling technique reveals differences between inner and outer leaflets at high bilayer curvature

(2012) Salvatore Chiantia et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Predicting hydrophilic drug encapsulation inside unilamellar liposomes

(2011) Xiaoming Xu et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Tail-anchored membrane protein insertion into the endoplasmic reticulum

(2011) Ramanujan S. Hegde et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Giant Vesicles: Preparations and Applications

(2010) Peter Walde et al.   CHEMBIOCHEM

How to Catch a Membrane Protein in Action: A Review of Functional Membrane Protein Immobilization Strategies and Their Applications

(2010) Virginie Früh et al.   CHEMICAL REVIEWS

Model systems for membrane fusion

(2010) Hana Robson Marsden et al.   CHEMICAL SOCIETY REVIEWS

Single-molecule dynamics of gating in a neurotransmitter transporter homologue

(2010) Yongfang Zhao et al.   NATURE

Thermal Stability of the Human Immunodeficiency Virus Type 1 (HIV-1) Receptors, CD4 and CXCR4, Reconstituted in Proteoliposomes

(2010) Mikhail A. Zhukovsky et al.   PLoS One

Precise detection of pH inside large unilamellar vesicles using membrane-impermeable dendritic porphyrin-based nanoprobes

(2009) Thom Leiding et al.   ANALYTICAL BIOCHEMISTRY

Solid-state NMR and functional studies on proteorhodopsin

(2009) Nicole Pfleger et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Lipid-anchored DNA mediates vesicle fusion as observed by lipid and content mixing

(2009) Yee-Hung M. Chan et al.   Biointerphases

Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy

(2009) Liguo Wang et al.   NATURE

Δψ and ΔpH are equivalent driving forces for proton transport through isolated F0 complexes of ATP synthases

(2008) Alexander Wiedenmann et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

SSM-based electrophysiology

(2008) Patrick Schulz et al.   METHODS

Membrane reconstitution of ABC transporters and assays of translocator function

(2008) Eric R Geertsma et al.   Nature Protocols