Review
Biochemistry & Molecular Biology
Pavel E. Z. Klier, Ryan Roo, Evan W. Miller
Summary: This article surveys recent advances in developing and deploying voltage-sensitive fluorescent indicators to interrogate organelle membrane potential in intact cells.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shunsuke Sawada, Masaru Yoshikawa, Keita Tsutsui, Tomoki Miyazaki, Keiko Kano, Emi Mishiro-Sato, Shinya Tsukiji
Summary: Small-molecule fluorescent probes based on the myrGC3Me motif were developed for simple and rapid staining of the Golgi in living cells with high specificity and no cytotoxicity. The probes allowed visualization of dynamic changes of the Golgi morphology induced by drug treatments and during cell division. These probes are useful tools for cell biological and diagnostic applications.
ACS CHEMICAL BIOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Junling Yin, Ling Huang, Luling Wu, Jiangfeng Li, Tony D. James, Weiying Lin
Summary: The microenvironment within specific organelles plays a vital role in cellular processes and physiological functions. Monitoring and understanding the variations in the microenvironment is essential for identifying disease development. Progress has been made in developing fluorescent probes to study the microenvironment within specific cellular regions, but comprehensive research on small-molecule fluorescent chemosensors for cellular microenvironments is still needed.
CHEMICAL SOCIETY REVIEWS
(2021)
Review
Chemistry, Multidisciplinary
Jamal Ghanam, Venkatesh Kumar Chetty, Xingfu Zhu, Xiaomin Liu, Marton Gelleri, Lennart Barthel, Dirk Reinhardt, Christoph Cremer, Basant Kumar Thakur
Summary: sEVs are nanovesicles with a size of 30-200 nm enriched with unique cargoes of nucleic acids, lipids, and proteins. They play a critical role in cell-to-cell communication but the mechanism of sEVs biogenesis and uptake is still unknown due to the lack of suitable imaging technologies. This review highlights the application of single-molecule localization microscopy (SMLM) in sEV biology and discusses different labeling strategies to study sEV biogenesis and their biomolecular interaction with recipient cells.
Review
Chemistry, Multidisciplinary
Ya-Lin Qi, Hai-Rong Wang, Li-Li Chen, Yong-Tao Duan, Sheng-Yu Yang, Hai-Liang Zhu
Summary: Ferroptosis is an iron-dependent, non-apoptotic form of programmed cell death involved in the development and progression of various diseases. Small-molecule fluorescent probes have been used to study ferroptosis by tracking biomolecules or microenvironment-related parameters in vitro and in vivo.
CHEMICAL SOCIETY REVIEWS
(2022)
Review
Cell Biology
Sheng-Yi Wu, Yi Shen, Irene Shkolnikov, Robert E. Campbell
Summary: This review provides an overview of the current landscape of genetically encodable and synthetic indicators for visualizing and detecting monatomic ion concentration in biological systems, serving as a valuable guide for researchers and tool developers.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Tianyi Qin, Bin Liu, Zhongyong Xu, Guangkai Yao, Hanhong Xu, Chen Zhao
Summary: Flavonols, as important natural botanical functional pigments, exhibit unique environmental-sensitive dual emissions as fluorophore skeleton for probe design. This review summarizes recent progress of flavonol-based fluorescent probes for diverse sensing applications, including detection of anions, cations, proteins, DNA, etc.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Brittany R. Benlian, Pavel E. Z. Klier, Kayli N. Martinez, Marie K. Schwinn, Thomas A. Kirkland, Evan W. Miller
Summary: Q-BOLT is a small-molecule enzyme pair for optical voltage sensing that utilizes quenching of bioluminescence to visualize changes in membrane potential in live cells. It combines three distinct readouts - QRET, BRET, and the ratio between bioluminescence emission and BRET - to accurately report membrane potential oscillations in HEK 293T cells and hiPSCs, without the need for excitation light.
Article
Chemistry, Medicinal
Sebastian Dekkers, Birgit Caspar, Joelle Goulding, Nicholas D. Kindon, Laura E. Kilpatrick, Leigh A. Stoddart, Stephen J. Briddon, Barrie Kellam, Stephen J. Hill, Michael J. Stocks
Summary: In this study, fluorescent probes based on previously reported small-molecule antagonists were designed and synthesized using classic medicinal chemistry approaches to investigate the pharmacology and cellular distribution of the CXCR4 receptor. Three distinct chemical classes of fluorescent probes were developed and shown to specifically bind to the CXCR4 receptor in a fluorescence-based NanoBRET binding assay (pKD ranging 6.6-7.1). These probes were also used in competition binding experiments and confocal microscopy to further explore the pharmacology and cellular distribution of CXCR4.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yilin Chang, Xiangwei Zhu, Yanan Shi, Yanan Liu, Ke Meng, Yanxun Li, Jingwei Xue, Lingyun Zhu, Jianqi Zhang, Huiqiong Zhou, Wei Ma, Zhixiang Wei, Kun Lu
Summary: Siloxane-based intermolecular compatibility is demonstrated as an efficient method for achieving ordered phase separation morphology and improving the photovoltaic performance of all-small-molecule organic solar cells. The introduction of siloxane in small-molecule donors leads to a nanostructured bicontinuous interpenetrating network with improved exciton dissociation and charge transport. The resulting device exhibits a power conversion efficiency of 16.4%.
ENERGY & ENVIRONMENTAL SCIENCE
(2022)
Review
Chemistry, Analytical
Haonan Li, Yuxi Fang, Junjie Yan, Xiangyu Ren, Chao Zheng, Bo Wu, Siyuan Wang, Zhanlin Li, Huiming Hua, Peng Wang, Dahong Li
Summary: Hydrogen sulfide (H2S) is considered as another gasotransmitter involved in important physiological and pathological processes. To real-time detect H2S in biological systems, numerous small-molecule fluorescent probes have been developed, with improvements in selectivity, response capability, and optical performance.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Rouba Nasreddine, Reine Nehme
Summary: Microscale thermophoresis (MST) is a sensitive biophysical tool that allows rapid and accurate determination of the dissociation constant (K-d) between enzymes and small molecules, aiding in the study of binding affinity between biomolecules. The study demonstrated that hyaluronidase maintained enzymatic activity after fluorescent labelling, but showed no affinity with flavonoid compounds under the tested conditions.
MICROCHEMICAL JOURNAL
(2021)
Review
Chemistry, Medicinal
Ting Li, Xuyang Chen, Kai Wang, Zhigang Hu
Summary: Sulfite is commonly used in food and beverages as an antioxidant and preservative, with abnormal levels in the body linked to various diseases. There is a need for a rapid, accurate, sensitive, and non-invasive method to monitor sulfite levels. Fluorescent probes offer advantages such as real-time imaging, ease of operation, cost-effectiveness, rapid response, and non-invasiveness for sulfite detection.
Article
Chemistry, Multidisciplinary
Yuan Zhu, Yuanmei Hu, Juanmei Zeng, Chaoxiang Chen, Shunhua Li, Yunbao Jiang
Summary: This study reports the development of high-performance SO2-responsive nanovesicles that undergo structural evolution upon stimulation, allowing for fluorescent tracking and quantitation of the stimuli.
CHINESE CHEMICAL LETTERS
(2022)
Article
Pharmacology & Pharmacy
Xiaohang Zheng, Jianxin Qiu, Wenjun Pan, Yuhang Gong, Weikang Zhang, Ting Jiang, Lihua Chen, Weifu Chen, Zhenghua Hong
Summary: The study found that selumetinib can protect chondrocytes by regulating necroptosis, preventing the progression of osteoarthritis and reducing osteoclast formation.
FRONTIERS IN PHARMACOLOGY
(2022)
